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The map label for this gene is thiI

Identifier: 15897271

GI number: 15897271

Start: 285759

End: 286874

Strand: Reverse

Name: thiI

Synonym: SSO0333

Alternate gene names: 15897271

Gene position: 286874-285759 (Counterclockwise)

Preceding gene: 15897272

Following gene: 15897260

Centisome position: 9.59

GC content: 35.13

Gene sequence:

>1116_bases
ATGTTAGTAATTATAAGACCATCAGGTGAAATTGCACTTAAATCGCCAAGGTCTAGGAGGAATTTTGAGTATACATTAAT
CAATAATATAAGAAATACTATAGGAGGAGGGAAAATATGGAGATCACAAGGAGTAATCCTATTAGAAGTTAACGATAATA
ACGCGAACATAGAGGCACTTAGCGAGGTATTCGGTATATCGTCTTTCTCGCCAGTAATAGCAATTAAATCAAATAATTTG
GAAGATATAGTTAACAAAGCTAAAGAAATTTTTGCGGAGATAGTTAAAGGCAAAATTTTCGCAGTAAGAGCAAAAAGAAT
AGGATCTCACAGTTTTACTAGCCTAGATGTTGAAAGAAGAGCTGGTGAAGCTTTATATCCATATTCTAAAGGAGTAGATC
TAGAGAACCCTGAAGTTGAGATATTTATAGAAATTAGAAATGAGATGACGTACTTCTATCATAAGGTTATTAAAGGACCG
AAGGGTTTACCGGTCGGTGTTGCAGGGAAGACAGTTGTGTTGTTTTCTGGTGGCATTGATTCACCTGTAGCCACATGGAT
GATGATGAAAAGAGGGTCAGTTCCCATTATTTTAAACTTCAATTTGGGCGGAGACCTACATAAGGAGTTAGTATTAAAAG
AATTAAATATGCTTAAAAGATGGAGTGGAGGACATAAATTAAAAATATTTATCGTAAAGGGTACAGATGTGTTTATTAAG
TTATCGCAAGTTGAGAGAAGAAGCCGTGTAGTCATGTTAAAGAGAGTGATGTATAAAACGGCTGAAAAGCTGTGCGAAAA
AACGAATGCTAAATCGATTACCACCGGGGAATCACTTTCGCAAGTATCGTCACAAACTATGGCTAATTTATACGTAACTG
AATATGGGATAAAGTATCCTATTTTTAGACCTCTAATTGGATTTGATAAGGAAGAAATCGTTGACATCGCAAGGAAAATT
GGAACTTACAAACACTCAATAAAACTTCCAGAATATTGCGCAATCTCAACTAAAGCTAGGACCTCTGAGGATCTTAATGA
AATATTAAAGAATGAAGAGAGGCTAAATGTTGATTACGAGAAGATACTTGAAAACTCGGAGGTAATTGAATTATGA

Upstream 100 bases:

>100_bases
ATTGATGGCTTTAAAATAATTTATTCTGAGTGCGAATGCGGACAAAAATTTTGTAGGCACGCAATACAACTATTTATGCA
CTACATGAGAGTGAGGGATC

Downstream 100 bases:

>100_bases
TCAGTCCTCACACGCGCACATCATTTATCAGTCCGGGGTTCACACATCATCTAAACCCCTTAAAGGATGAGATTCGTTCT
AAACTTTTATCATTAATAAA

Product: thiamine biosynthesis protein thiI (thiI)

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLVIIRPSGEIALKSPRSRRNFEYTLINNIRNTIGGGKIWRSQGVILLEVNDNNANIEALSEVFGISSFSPVIAIKSNNL
EDIVNKAKEIFAEIVKGKIFAVRAKRIGSHSFTSLDVERRAGEALYPYSKGVDLENPEVEIFIEIRNEMTYFYHKVIKGP
KGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLKIFIVKGTDVFIK
LSQVERRSRVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQVSSQTMANLYVTEYGIKYPIFRPLIGFDKEEIVDIARKI
GTYKHSIKLPEYCAISTKARTSEDLNEILKNEERLNVDYEKILENSEVIEL

Sequences:

>Translated_371_residues
MLVIIRPSGEIALKSPRSRRNFEYTLINNIRNTIGGGKIWRSQGVILLEVNDNNANIEALSEVFGISSFSPVIAIKSNNL
EDIVNKAKEIFAEIVKGKIFAVRAKRIGSHSFTSLDVERRAGEALYPYSKGVDLENPEVEIFIEIRNEMTYFYHKVIKGP
KGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLKIFIVKGTDVFIK
LSQVERRSRVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQVSSQTMANLYVTEYGIKYPIFRPLIGFDKEEIVDIARKI
GTYKHSIKLPEYCAISTKARTSEDLNEILKNEERLNVDYEKILENSEVIEL
>Mature_371_residues
MLVIIRPSGEIALKSPRSRRNFEYTLINNIRNTIGGGKIWRSQGVILLEVNDNNANIEALSEVFGISSFSPVIAIKSNNL
EDIVNKAKEIFAEIVKGKIFAVRAKRIGSHSFTSLDVERRAGEALYPYSKGVDLENPEVEIFIEIRNEMTYFYHKVIKGP
KGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLKIFIVKGTDVFIK
LSQVERRSRVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQVSSQTMANLYVTEYGIKYPIFRPLIGFDKEEIVDIARKI
GTYKHSIKLPEYCAISTKARTSEDLNEILKNEERLNVDYEKILENSEVIEL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=364, Percent_Identity=33.2417582417582, Blast_Score=184, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_SULSO (Q980G6)

Other databases:

- EMBL:   AE006641
- PIR:   C90176
- RefSeq:   NP_341876.1
- ProteinModelPortal:   Q980G6
- SMR:   Q980G6
- GeneID:   1455476
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0333
- NMPDR:   fig|273057.1.peg.299
- HOGENOM:   HBG646072
- OMA:   RREWGRI
- ProtClustDB:   CLSK267757
- BioCyc:   SSOL273057:SSO0333-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 41848; Mature: 41848

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS51165 THUMP

Important sites: BINDING 254-254 BINDING 276-276 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIIRPSGEIALKSPRSRRNFEYTLINNIRNTIGGGKIWRSQGVILLEVNDNNANIEAL
CEEEEECCCCEEECCCCCCCCEEEEEHHHHHHHCCCCEEEECCCEEEEEECCCCCCHHHH
SEVFGISSFSPVIAIKSNNLEDIVNKAKEIFAEIVKGKIFAVRAKRIGSHSFTSLDVERR
HHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCEEEEHHHH
AGEALYPYSKGVDLENPEVEIFIEIRNEMTYFYHKVIKGPKGLPVGVAGKTVVLFSGGID
CCCEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCC
SPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLKIFIVKGTDVFIK
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEE
LSQVERRSRVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQVSSQTMANLYVTEYGIKYP
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHEEEEECCCCCC
IFRPLIGFDKEEIVDIARKIGTYKHSIKLPEYCAISTKARTSEDLNEILKNEERLNVDYE
HHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCEEECCCCCCHHHHHHHHCCCHHCCCCHH
KILENSEVIEL
HHCCCCCEECC
>Mature Secondary Structure
MLVIIRPSGEIALKSPRSRRNFEYTLINNIRNTIGGGKIWRSQGVILLEVNDNNANIEAL
CEEEEECCCCEEECCCCCCCCEEEEEHHHHHHHCCCCEEEECCCEEEEEECCCCCCHHHH
SEVFGISSFSPVIAIKSNNLEDIVNKAKEIFAEIVKGKIFAVRAKRIGSHSFTSLDVERR
HHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCEEEEHHHH
AGEALYPYSKGVDLENPEVEIFIEIRNEMTYFYHKVIKGPKGLPVGVAGKTVVLFSGGID
CCCEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCC
SPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLKIFIVKGTDVFIK
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEE
LSQVERRSRVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQVSSQTMANLYVTEYGIKYP
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHEEEEECCCCCC
IFRPLIGFDKEEIVDIARKIGTYKHSIKLPEYCAISTKARTSEDLNEILKNEERLNVDYE
HHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCEEECCCCCCHHHHHHHHCCCHHCCCCHH
KILENSEVIEL
HHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726