Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 15897185

GI number: 15897185

Start: 206480

End: 207904

Strand: Reverse

Name: purB [H]

Synonym: SSO0240

Alternate gene names: 15897185

Gene position: 207904-206480 (Counterclockwise)

Preceding gene: 15897191

Following gene: 15897184

Centisome position: 6.95

GC content: 34.39

Gene sequence:

>1425_bases
GTGAGATTACAGTATAAGTTAATAAATGTTGAACATACTTTACAATTAGCCATGGAGGATAGTATATGTCCTTTAGAATG
GAGATACGGTAGTAATGAGATGAGAAAGTTTTTCAGTAGAGAAGAAATCTTGAAAAGGAGAATTCAAGTAGAAATAGCGT
TATTAAAAGCATTAACCAAATATGGTTTAGTAAAAGAGGAAGACGTTCAAAAAGTTGAGAAAGCTTCATTAACAATAAGA
CCAGAGGAAGTAGATGAACTTGAATCAAAAATTGGTCATGATGTAATGGCTATGGTAGTCTTAATGGCAGAAAGAAGTGG
AGATTCTGGAAGATACGTTCATTTTGGGGCAACAAGTTATGATATTGTAGATACAGCTTATGCGTTAGTATTTAGAGACG
CAATAAGATTAGTAAAGACAAAAGTAGTAGAAATATTGGAAATGATGGTTAGTTTAGCAATTAAGTATAAAGATTTAGCA
ATGGTAGGAAGAACTCATGGGCAGCATGCATTGCCTATAACATTAGGCTTTAAAATTGCAAATTATATCTATGAATTTTC
TAGATCACTAGAGAGGTTAAATGAGGTTGAGAAAAGAATAGTAAAAGGTAAAATGGCAGGTGCAGTTGGCACGATGGCAG
CTTGGGGCAATATAGGGTTAGAAATAGAAGAGAATGTTATGAAATTTTTAGGATTAAGACCTCATGAGATTTCAACTCAA
GTTGCTCCACGAGACGCATTTGCAGAATTAACTTCTGATCTTGCAATTTTGGCATCTCAATGTGATCGATTCGCATTAGA
AGTTAGGGAACTAATGAGACCGGAAATAAATGAAATAAGTGAAGGCGTAGAAGGTCGTGTAGGAAGTAGTACTATGCCTC
AGAAGGAAAATCCAGTCACGTCAGAAAAGATTGGGGGTTTAGCAAAAGTTTTACGCTCTCTTGTCGTAGCGTCACTAGAA
AATATTCCATTATGGCACGAAAGAGATCTGACTAACAGTTCGTCAGAGAGGATAATAATATCACATGAATTTCTAGTTAT
TGACGAAATATTAGATAGTATGATAAGTCTTTTGAGGAATTTAAGGGTTAACTTCGAGAATATTGAAAAGAATTTGAACT
TAACTAAAGGACAAATAATGGCAGAAAGTCTGATGATAAACTTAACCTTAAAGGGCATGAAAAGGCATGAAGCTCATCAA
TACGTTAACAAACTGACGTCAAAAGTAAGACAAACTGGAAAAACGTTATTAGAGGTTTGCCTAGAGGATAGTTTAATTTT
AAGTTATTTCTCTAAGCAAGAACTTGAGAACGTACTTAATCCTAAAAACTACTTGGGTTCCTATGATAAATTAATAGAGA
GAGCTATTAGCTATGCAAAAGGCGTAATCAAAGGTTCAGAAATACGAGAAGCTCAAGATAGATGA

Upstream 100 bases:

>100_bases
TTTATTATCAGTTATTACCATAGTTTGAAAACCTTCCTTCTTGGCTCCATGTAAGATCTGTAATGCAGAATGACTACCGA
TTGTTGCGATTATCATTGTT

Downstream 100 bases:

>100_bases
TATAAGGTTCGCATTTTAAGCTTTAAATAATCCATTGAATATATAGTAAAATGATGCCGCCGTAGCTCAGCCTGGTTAGA
GCGCCGGCCTCGTACCCAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRLQYKLINVEHTLQLAMEDSICPLEWRYGSNEMRKFFSREEILKRRIQVEIALLKALTKYGLVKEEDVQKVEKASLTIR
PEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLA
MVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKMAGAVGTMAAWGNIGLEIEENVMKFLGLRPHEISTQ
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLE
NIPLWHERDLTNSSSERIIISHEFLVIDEILDSMISLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQ
YVNKLTSKVRQTGKTLLEVCLEDSLILSYFSKQELENVLNPKNYLGSYDKLIERAISYAKGVIKGSEIREAQDR

Sequences:

>Translated_474_residues
MRLQYKLINVEHTLQLAMEDSICPLEWRYGSNEMRKFFSREEILKRRIQVEIALLKALTKYGLVKEEDVQKVEKASLTIR
PEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLA
MVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKMAGAVGTMAAWGNIGLEIEENVMKFLGLRPHEISTQ
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLE
NIPLWHERDLTNSSSERIIISHEFLVIDEILDSMISLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQ
YVNKLTSKVRQTGKTLLEVCLEDSLILSYFSKQELENVLNPKNYLGSYDKLIERAISYAKGVIKGSEIREAQDR
>Mature_474_residues
MRLQYKLINVEHTLQLAMEDSICPLEWRYGSNEMRKFFSREEILKRRIQVEIALLKALTKYGLVKEEDVQKVEKASLTIR
PEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLA
MVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKMAGAVGTMAAWGNIGLEIEENVMKFLGLRPHEISTQ
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLE
NIPLWHERDLTNSSSERIIISHEFLVIDEILDSMISLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQ
YVNKLTSKVRQTGKTLLEVCLEDSLILSYFSKQELENVLNPKNYLGSYDKLIERAISYAKGVIKGSEIREAQDR

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=450, Percent_Identity=26.6666666666667, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=26.8229166666667, Blast_Score=122, Evalue=9e-28,
Organism=Escherichia coli, GI1787376, Length=464, Percent_Identity=26.5086206896552, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1790398, Length=433, Percent_Identity=24.9422632794457, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=375, Percent_Identity=25.0666666666667, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=306, Percent_Identity=25.8169934640523, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17553882, Length=349, Percent_Identity=24.0687679083095, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI32565146, Length=302, Percent_Identity=24.8344370860927, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=459, Percent_Identity=26.3616557734205, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24647570, Length=449, Percent_Identity=27.1714922048998, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640177, Length=312, Percent_Identity=25.3205128205128, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640179, Length=312, Percent_Identity=25.3205128205128, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221473854, Length=411, Percent_Identity=21.8978102189781, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI78706858, Length=411, Percent_Identity=21.8978102189781, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI78710009, Length=339, Percent_Identity=25.0737463126844, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53849; Mature: 53849

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQYKLINVEHTLQLAMEDSICPLEWRYGSNEMRKFFSREEILKRRIQVEIALLKALTK
CCEEEEEECHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLVKEEDVQKVEKASLTIRPEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSY
HCCCCHHHHHHHHHHCCEECHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEEEECCCCH
DIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLAMVGRTHGQHALPITLGFKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
NYIYEFSRSLERLNEVEKRIVKGKMAGAVGTMAAWGNIGLEIEENVMKFLGLRPHEISTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCC
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCC
SEKIGGLAKVLRSLVVASLENIPLWHERDLTNSSSERIIISHEFLVIDEILDSMISLLRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQYVNKLTSKVRQTGKTLLEVC
HCCCHHHHHHCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDSLILSYFSKQELENVLNPKNYLGSYDKLIERAISYAKGVIKGSEIREAQDR
HHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MRLQYKLINVEHTLQLAMEDSICPLEWRYGSNEMRKFFSREEILKRRIQVEIALLKALTK
CCEEEEEECHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLVKEEDVQKVEKASLTIRPEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSY
HCCCCHHHHHHHHHHCCEECHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEEEECCCCH
DIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLAMVGRTHGQHALPITLGFKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
NYIYEFSRSLERLNEVEKRIVKGKMAGAVGTMAAWGNIGLEIEENVMKFLGLRPHEISTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCC
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCC
SEKIGGLAKVLRSLVVASLENIPLWHERDLTNSSSERIIISHEFLVIDEILDSMISLLRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQYVNKLTSKVRQTGKTLLEVC
HCCCHHHHHHCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDSLILSYFSKQELENVLNPKNYLGSYDKLIERAISYAKGVIKGSEIREAQDR
HHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]