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The map label for this gene is truD

Identifier: 15897125

GI number: 15897125

Start: 145235

End: 146368

Strand: Reverse

Name: truD

Synonym: SSO0174

Alternate gene names: 15897125

Gene position: 146368-145235 (Counterclockwise)

Preceding gene: 15897126

Following gene: 15897122

Centisome position: 4.89

GC content: 31.92

Gene sequence:

>1134_bases
GTGACTCCTCATGAAATAGACATTGCGTTAGGTATGGAAAAATATTATTATGAAGACTGGAACGAGTTAGACGGAACAAT
TGAAAGACCAAACGGGTTTAAAGTTATAGAAGAGATTGACTTTAAGCCAGCTCAAGATTGGAAAGGCGAGACAAAAGGTA
AATATGCGGTATTCTTACTTACAAAATGGGGAATAGATCATTTTTCCGCTATATCCGAAGTTCAGAAAGTACTACATTCT
AAAGTAAATTATATAGGTATTAAGGATGCTAATGCGATAACATCTCAGTTAGTATATATACCGTTAAACGAAAAACAAGA
ACTGATTGAGAAATATCAATCAAGGAGTTTTATTCTAAAGTTCCTCGGATTTTCTTCAAAAAAACTAAATCATACTGGAA
ATATATTTGAAATATCCCTCTCAATATCCGACTTCGATATAGTCATAGAGAGAATAGAACAAATTAAGAAAAATCCATAT
CTCCCAGCTTTTATAGGCTATCAACGTTTTGGTACCCGGAGGCCTATTACACATTTAATAGGGAAATATCTTTTGAGAAG
AGACTGGGAGAAGGCATTTTATCTGATTTTGACATATCCTTTTCTTTCCGAGTCAAAAGAAACCATTGACATAAGAAAGT
TAATAATGGAAGGCGATTTTAAAGAAGCAGTTAGATCGATTCCGTCCAAGTTTAAGCAAGAGAAATTGTTACTGAAGAAC
TATATGAGATTTAATTCTTATTATCTAGCCCTTAAAAGTAGTTTCATTCCAATCTCGTTATATTTAGACGCATATCAAAG
TTATTTATTTAATCTGTATTTATCCAGAAAGCTAGACGAATATAAGAACCTTAATGATAAGGTTAACTTGCTTATAAGAA
TACCTATCTATTTTAATAACTGTGATGATGTTTGCAAGGAAATTTATCTGGATGAGGGAATAGAACGAAATTTTTTCAAA
TTACAAGAATTCAAAATCTCCCTAAGAGACCTCGTGAGGAAGGCGTTTATGAATATAAGAGATTTAAAAGTTAACGAGGA
AACTAAAACAATATCTTTTGTATTAGAAAGAGGAATGTACGCTACAATATTACTTAGGGAAATCTTAAGAGGAGATCCAA
GAAAATTCACTTAA

Upstream 100 bases:

>100_bases
CAGGAAAGACTCAATTAGAACCCGGCACTATAACGTGTTTAGGTATCGGGCCTGCACCAGAAAATTTAGTAGATTCTATC
ACGGGTGATCTAAAATTACT

Downstream 100 bases:

>100_bases
CTTTTATGGTGTTAGTCTCTTCTTATCTCTAGGGAATGGTACTACTTCCTTCACGCTCTGAAGACCAGTTAACATTAACA
TTACTCTTGCTAATCCCATT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTPHEIDIALGMEKYYYEDWNELDGTIERPNGFKVIEEIDFKPAQDWKGETKGKYAVFLLTKWGIDHFSAISEVQKVLHS
KVNYIGIKDANAITSQLVYIPLNEKQELIEKYQSRSFILKFLGFSSKKLNHTGNIFEISLSISDFDIVIERIEQIKKNPY
LPAFIGYQRFGTRRPITHLIGKYLLRRDWEKAFYLILTYPFLSESKETIDIRKLIMEGDFKEAVRSIPSKFKQEKLLLKN
YMRFNSYYLALKSSFIPISLYLDAYQSYLFNLYLSRKLDEYKNLNDKVNLLIRIPIYFNNCDDVCKEIYLDEGIERNFFK
LQEFKISLRDLVRKAFMNIRDLKVNEETKTISFVLERGMYATILLREILRGDPRKFT

Sequences:

>Translated_377_residues
MTPHEIDIALGMEKYYYEDWNELDGTIERPNGFKVIEEIDFKPAQDWKGETKGKYAVFLLTKWGIDHFSAISEVQKVLHS
KVNYIGIKDANAITSQLVYIPLNEKQELIEKYQSRSFILKFLGFSSKKLNHTGNIFEISLSISDFDIVIERIEQIKKNPY
LPAFIGYQRFGTRRPITHLIGKYLLRRDWEKAFYLILTYPFLSESKETIDIRKLIMEGDFKEAVRSIPSKFKQEKLLLKN
YMRFNSYYLALKSSFIPISLYLDAYQSYLFNLYLSRKLDEYKNLNDKVNLLIRIPIYFNNCDDVCKEIYLDEGIERNFFK
LQEFKISLRDLVRKAFMNIRDLKVNEETKTISFVLERGMYATILLREILRGDPRKFT
>Mature_376_residues
TPHEIDIALGMEKYYYEDWNELDGTIERPNGFKVIEEIDFKPAQDWKGETKGKYAVFLLTKWGIDHFSAISEVQKVLHSK
VNYIGIKDANAITSQLVYIPLNEKQELIEKYQSRSFILKFLGFSSKKLNHTGNIFEISLSISDFDIVIERIEQIKKNPYL
PAFIGYQRFGTRRPITHLIGKYLLRRDWEKAFYLILTYPFLSESKETIDIRKLIMEGDFKEAVRSIPSKFKQEKLLLKNY
MRFNSYYLALKSSFIPISLYLDAYQSYLFNLYLSRKLDEYKNLNDKVNLLIRIPIYFNNCDDVCKEIYLDEGIERNFFKL
QEFKISLRDLVRKAFMNIRDLKVNEETKTISFVLERGMYATILLREILRGDPRKFT

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI50727002, Length=244, Percent_Identity=26.6393442622951, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324817, Length=282, Percent_Identity=28.7234042553192, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_SULSO (Q980V2)

Other databases:

- EMBL:   AE006641
- PIR:   A90158
- RefSeq:   NP_341730.1
- ProteinModelPortal:   Q980V2
- SMR:   Q980V2
- GeneID:   1453347
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0174
- NMPDR:   fig|273057.1.peg.153
- HOGENOM:   HBG553495
- OMA:   AYIGYQR
- ProtClustDB:   CLSK802596
- BioCyc:   SSOL273057:SSO0174-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 44738; Mature: 44607

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 89-89

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPHEIDIALGMEKYYYEDWNELDGTIERPNGFKVIEEIDFKPAQDWKGETKGKYAVFLL
CCCCEEEEEECHHHHHHCCHHHCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEE
TKWGIDHFSAISEVQKVLHSKVNYIGIKDANAITSQLVYIPLNEKQELIEKYQSRSFILK
EECCCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHEEEEEECCCHHHHHHHHHHHHHHHH
FLGFSSKKLNHTGNIFEISLSISDFDIVIERIEQIKKNPYLPAFIGYQRFGTRRPITHLI
HHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHH
GKYLLRRDWEKAFYLILTYPFLSESKETIDIRKLIMEGDFKEAVRSIPSKFKQEKLLLKN
HHHHHHHCHHHEEEEEEECCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YMRFNSYYLALKSSFIPISLYLDAYQSYLFNLYLSRKLDEYKNLNDKVNLLIRIPIYFNN
HHHHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECC
CDDVCKEIYLDEGIERNFFKLQEFKISLRDLVRKAFMNIRDLKVNEETKTISFVLERGMY
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCEEECCHHHHHHHHHHCCHH
ATILLREILRGDPRKFT
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TPHEIDIALGMEKYYYEDWNELDGTIERPNGFKVIEEIDFKPAQDWKGETKGKYAVFLL
CCCEEEEEECHHHHHHCCHHHCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEE
TKWGIDHFSAISEVQKVLHSKVNYIGIKDANAITSQLVYIPLNEKQELIEKYQSRSFILK
EECCCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHEEEEEECCCHHHHHHHHHHHHHHHH
FLGFSSKKLNHTGNIFEISLSISDFDIVIERIEQIKKNPYLPAFIGYQRFGTRRPITHLI
HHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHH
GKYLLRRDWEKAFYLILTYPFLSESKETIDIRKLIMEGDFKEAVRSIPSKFKQEKLLLKN
HHHHHHHCHHHEEEEEEECCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YMRFNSYYLALKSSFIPISLYLDAYQSYLFNLYLSRKLDEYKNLNDKVNLLIRIPIYFNN
HHHHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECC
CDDVCKEIYLDEGIERNFFKLQEFKISLRDLVRKAFMNIRDLKVNEETKTISFVLERGMY
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCEEECCHHHHHHHHHHCCHH
ATILLREILRGDPRKFT
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726