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The map label for this gene is ilvB-1 [H]

Identifier: 15897120

GI number: 15897120

Start: 140783

End: 142498

Strand: Reverse

Name: ilvB-1 [H]

Synonym: SSO0170

Alternate gene names: 15897120

Gene position: 142498-140783 (Counterclockwise)

Preceding gene: 15897122

Following gene: 15897119

Centisome position: 4.76

GC content: 35.78

Gene sequence:

>1716_bases
TTGACACCCGTTATTTCGCGGGTTCGAATCCCGCCCCCGGCTTTTAGAAGAGATTTAAAAAGTAGTTCCAATTATAATGT
TAGTATGAGCCAGCCCAAAAGAAAAGAGGAGACTGTAGGCGTAGAAATGAAAGGCGAAGAAGCCCTTGCATACGTTTTAA
ATGATATTGGCTTAACTAAGGTATTTACAACTTACTCCCTACCAAATATTGTTAAGGAAATGTTAAAGAAGTACAATATT
GAAGTTGATTTTTCTATTTCAGTAAAAGACGCAAGCTGGCTAGCTTATGCTTATGCAATGGAAAATAATTCAGTAGGGAC
TATAATTCAGATACCGGGCTCGAAGTTAACTGATGCAGTAGATGTTATAGCTCAAGCCTATATGGAGTCAGTTCCCCTTC
TTATAATATCTAGCGTAAGATCACATAAAGACACAGGAAGGGCCAGAATTGGTGAATTTAGAACGATCGATGATTTATCA
AACATTTTATCTCCAATAATTAAGACTAAAGAAAGAGTTATCAGTATAGAAGAAATAACTGTTACAATAGAGAAAGCCTA
TAAGGAAGCAGTTAGTAATAGACCAAGACCTTCTTATGTTGAAATATCTGAAGACTTATTCAGGGCAAAAGCTTATCCGT
TATCCACTGCGGGGCAAAAGCCAGAGAAGAGGACTCCAGATAAGAATAGTGTAGCCAAAGTAGCTGAACTTTTAACTAAT
GCTAAATTACCAGTTATAATTGCGGGATATGGAGTAGTTTTAAGCGACGCAGAAGACATGTTAGTCGAACTGGCTGAATT
AATAGATTCACCAGTAGTTACTACATTTAAGGCTAAAGGTTCAATACCATCTAACCATAAATTATTTGCTGGAGAAGGAT
TAGGTGCCTTTAGCACTAGTGCCGCAAACTATCTAATTGAGAATGCTGATGTAATACTAGCGTTAGGCACTAGGTTTACT
CAGTTAAGTACAGCTGGCTGGTCATTAAAGTATAAAGGCATCCTAGTGCATAACAATGTTGATGGTGAGGATATAGGCAA
AGTTTTCATGCCTCATGTTCCAATAGTAGCTGATACCGGGTTATTCTTAAGAGAACTATTAACTCAATTAAAGGCTAAGA
TAAAGGAGAAGATAAATAGAGGGGCTAGTGATATTATTTATAAAACTCAACGTCAAGCATACCCAATTACATCTCATAAT
GATATATGGCCTATAGATGTGGTGAAAATGCTAAGCAGTATAGGTGGCTTTGAGAAAGTCTATGTGGATATTTCGGCTAC
AACAATAGATTTAGTTAGATTACCGATAAATGCTAAGAAGACGTGGTATACTGCAGAATCTTTACTAGAGAGAGGTATCG
CGGTAGGTGGTATTATAGCTTCTAAATACGTTGCATATGGAATTACTGATATAGAGGGAATATTACCTCATTTATCATTA
CTAAAATATAAGATGGATAAGATTAAAGGAAAGTTAATTATATTAAATGATGGGGGCGCAAATTACATTGAAGTTTCTAA
CTCTGATTTACCTACTATTGCTAGATCACAGACTAGTTTCAATGCCAATTTTGATGAGATTGCAGAAAAAGCCTTAGGTG
GTGTAACTGTTAATACATTAACTGAGTTAGAAGAGGCTTTGAAATCTGTCGATAAGAAAATCATAAATGTAAAGATTGAT
CCTAACTTCGAGTCGGTAATACTTTCAAGAATTTAA

Upstream 100 bases:

>100_bases
AAGTAGTTTTTAATAAGTTATTATTGTTGCCGGGGTGGCCGAGTGGTCTAAGGCGGCACTACCAGCCCATCGGTAGCGCT
AGGGGCCGCAGTGGTACTAG

Downstream 100 bases:

>100_bases
TTAAGTGAAGAGTATTAATTAAGGCTAATTTTATTTTGTCTTATTCTGAAGTTTAAAATATGAACGATAAGTCTTCAAGT
CAAATTTCAATCAGAAGGGC

Product: acetolactate synthase large subunit IlvB

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTPVISRVRIPPPAFRRDLKSSSNYNVSMSQPKRKEETVGVEMKGEEALAYVLNDIGLTKVFTTYSLPNIVKEMLKKYNI
EVDFSISVKDASWLAYAYAMENNSVGTIIQIPGSKLTDAVDVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLS
NILSPIIKTKERVISIEEITVTIEKAYKEAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNSVAKVAELLTN
AKLPVIIAGYGVVLSDAEDMLVELAELIDSPVVTTFKAKGSIPSNHKLFAGEGLGAFSTSAANYLIENADVILALGTRFT
QLSTAGWSLKYKGILVHNNVDGEDIGKVFMPHVPIVADTGLFLRELLTQLKAKIKEKINRGASDIIYKTQRQAYPITSHN
DIWPIDVVKMLSSIGGFEKVYVDISATTIDLVRLPINAKKTWYTAESLLERGIAVGGIIASKYVAYGITDIEGILPHLSL
LKYKMDKIKGKLIILNDGGANYIEVSNSDLPTIARSQTSFNANFDEIAEKALGGVTVNTLTELEEALKSVDKKIINVKID
PNFESVILSRI

Sequences:

>Translated_571_residues
MTPVISRVRIPPPAFRRDLKSSSNYNVSMSQPKRKEETVGVEMKGEEALAYVLNDIGLTKVFTTYSLPNIVKEMLKKYNI
EVDFSISVKDASWLAYAYAMENNSVGTIIQIPGSKLTDAVDVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLS
NILSPIIKTKERVISIEEITVTIEKAYKEAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNSVAKVAELLTN
AKLPVIIAGYGVVLSDAEDMLVELAELIDSPVVTTFKAKGSIPSNHKLFAGEGLGAFSTSAANYLIENADVILALGTRFT
QLSTAGWSLKYKGILVHNNVDGEDIGKVFMPHVPIVADTGLFLRELLTQLKAKIKEKINRGASDIIYKTQRQAYPITSHN
DIWPIDVVKMLSSIGGFEKVYVDISATTIDLVRLPINAKKTWYTAESLLERGIAVGGIIASKYVAYGITDIEGILPHLSL
LKYKMDKIKGKLIILNDGGANYIEVSNSDLPTIARSQTSFNANFDEIAEKALGGVTVNTLTELEEALKSVDKKIINVKID
PNFESVILSRI
>Mature_570_residues
TPVISRVRIPPPAFRRDLKSSSNYNVSMSQPKRKEETVGVEMKGEEALAYVLNDIGLTKVFTTYSLPNIVKEMLKKYNIE
VDFSISVKDASWLAYAYAMENNSVGTIIQIPGSKLTDAVDVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLSN
ILSPIIKTKERVISIEEITVTIEKAYKEAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNSVAKVAELLTNA
KLPVIIAGYGVVLSDAEDMLVELAELIDSPVVTTFKAKGSIPSNHKLFAGEGLGAFSTSAANYLIENADVILALGTRFTQ
LSTAGWSLKYKGILVHNNVDGEDIGKVFMPHVPIVADTGLFLRELLTQLKAKIKEKINRGASDIIYKTQRQAYPITSHND
IWPIDVVKMLSSIGGFEKVYVDISATTIDLVRLPINAKKTWYTAESLLERGIAVGGIIASKYVAYGITDIEGILPHLSLL
KYKMDKIKGKLIILNDGGANYIEVSNSDLPTIARSQTSFNANFDEIAEKALGGVTVNTLTELEEALKSVDKKIINVKIDP
NFESVILSRI

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=299, Percent_Identity=26.4214046822742, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI87081685, Length=285, Percent_Identity=29.1228070175439, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786717, Length=333, Percent_Identity=26.7267267267267, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790104, Length=389, Percent_Identity=25.1928020565553, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788716, Length=496, Percent_Identity=22.7822580645161, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=391, Percent_Identity=23.7851662404092, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320816, Length=513, Percent_Identity=23.0019493177388, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=338, Percent_Identity=23.6686390532544, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62805; Mature: 62674

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVISRVRIPPPAFRRDLKSSSNYNVSMSQPKRKEETVGVEMKGEEALAYVLNDIGLTK
CCCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCHHHHCCEEECCCHHHHHHHHHCCHHH
VFTTYSLPNIVKEMLKKYNIEVDFSISVKDASWLAYAYAMENNSVGTIIQIPGSKLTDAV
HHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCCEEEEECCCCHHHHHH
DVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLSNILSPIIKTKERVISIEEIT
HHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHE
VTIEKAYKEAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNSVAKVAELLTN
EHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
AKLPVIIAGYGVVLSDAEDMLVELAELIDSPVVTTFKAKGSIPSNHKLFAGEGLGAFSTS
CCCCEEEEECCEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCHHH
AANYLIENADVILALGTRFTQLSTAGWSLKYKGILVHNNVDGEDIGKVFMPHVPIVADTG
HHHHHEECCCEEEEECCCHHEEECCCCEEEEEEEEEECCCCHHHHHHHHCCCCCEEECCC
LFLRELLTQLKAKIKEKINRGASDIIYKTQRQAYPITSHNDIWPIDVVKMLSSIGGFEKV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
YVDISATTIDLVRLPINAKKTWYTAESLLERGIAVGGIIASKYVAYGITDIEGILPHLSL
EEEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKYKMDKIKGKLIILNDGGANYIEVSNSDLPTIARSQTSFNANFDEIAEKALGGVTVNTL
HHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
TELEEALKSVDKKIINVKIDPNFESVILSRI
HHHHHHHHHHHHEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure 
TPVISRVRIPPPAFRRDLKSSSNYNVSMSQPKRKEETVGVEMKGEEALAYVLNDIGLTK
CCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCHHHHCCEEECCCHHHHHHHHHCCHHH
VFTTYSLPNIVKEMLKKYNIEVDFSISVKDASWLAYAYAMENNSVGTIIQIPGSKLTDAV
HHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCCEEEEECCCCHHHHHH
DVIAQAYMESVPLLIISSVRSHKDTGRARIGEFRTIDDLSNILSPIIKTKERVISIEEIT
HHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHE
VTIEKAYKEAVSNRPRPSYVEISEDLFRAKAYPLSTAGQKPEKRTPDKNSVAKVAELLTN
EHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
AKLPVIIAGYGVVLSDAEDMLVELAELIDSPVVTTFKAKGSIPSNHKLFAGEGLGAFSTS
CCCCEEEEECCEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCHHH
AANYLIENADVILALGTRFTQLSTAGWSLKYKGILVHNNVDGEDIGKVFMPHVPIVADTG
HHHHHEECCCEEEEECCCHHEEECCCCEEEEEEEEEECCCCHHHHHHHHCCCCCEEECCC
LFLRELLTQLKAKIKEKINRGASDIIYKTQRQAYPITSHNDIWPIDVVKMLSSIGGFEKV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
YVDISATTIDLVRLPINAKKTWYTAESLLERGIAVGGIIASKYVAYGITDIEGILPHLSL
EEEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKYKMDKIKGKLIILNDGGANYIEVSNSDLPTIARSQTSFNANFDEIAEKALGGVTVNTL
HHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
TELEEALKSVDKKIINVKIDPNFESVILSRI
HHHHHHHHHHHHEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]