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The map label for this gene is ntH-1 [H]

Identifier: 15897075

GI number: 15897075

Start: 95139

End: 95849

Strand: Reverse

Name: ntH-1 [H]

Synonym: SSO0116

Alternate gene names: 15897075

Gene position: 95849-95139 (Counterclockwise)

Preceding gene: 15897077

Following gene: 15897074

Centisome position: 3.2

GC content: 34.04

Gene sequence:

>711_bases
ATGAAGTGCACTGCTGAAACGATTTTCCATAAACTTTCTGCTACCTATATTATAAAAGAGGAGGATTTTATTGCGTATTA
TGTCTGGCTTAAGACCAAAGATTGTTTTAAAGTACTTGTAGCAACTATTTTATCTCAAAATTCTACAGATAAATCAGCCA
TTAAAGCCTATCTAGAGTTAGAAAGAAAAGTTGGTGTTACTCCAGAGAAATTATCTAACGCCAATTTGGCTGACATAGAA
AGCGCGTTAAAGATTTCCGGTCTTTATAGAACTAAGGCTAAAAGATTAAAGGAAATTTCAAGGATAATTCTTGAGAGGTA
TAATGGATTAATAGATAGTTTGTTAAATACTAGCAATGCGAGAGATGAATTATTAAAACTTGAGGGAATTGGAGAGAAGA
CTGCGGATGTAGTGCTATTGACTTGTTATGGATATTATGGATATAAGGTCTTTCCGGTTGATACGCACATAACTAGAGTC
AGCAAAAGGTTAGGGATAGTCCCCACTAACGCTAAATATAGTTTAATTTCATCTACTTTAAAAGAACTTTTTTCAGCCTA
TGATCTCCTCCATCTTCATCACATGTTAATTGCACATGGTAGACAAACTTGTAAGGCTAGGAAACCTTTATGTAATTCTT
GTATAATTAAAGAATGTTGTGAGTACTATTCGCATAGAGATGGAGAAGCTTGGAGATCTAATACCTCATGA

Upstream 100 bases:

>100_bases
AGCACCTTCCTTTATTGCGTTAAGGACCATCTCTATTTCGTACATCAAATAGTAAGTTAGTTTAAGAATTCCTTATAAGC
CAAAGTGCCTAATCAAATTC

Downstream 100 bases:

>100_bases
GGATATATTAATAGATAAAGTTGTCAATATCTCTTTTGAAGTTCAGAAAATAAGGGCTATAAAACCAATAATAGTAGATG
AAAACACACATGTCATCTTA

Product: DNA endonuclease III, (ntH-1)

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKCTAETIFHKLSATYIIKEEDFIAYYVWLKTKDCFKVLVATILSQNSTDKSAIKAYLELERKVGVTPEKLSNANLADIE
SALKISGLYRTKAKRLKEISRIILERYNGLIDSLLNTSNARDELLKLEGIGEKTADVVLLTCYGYYGYKVFPVDTHITRV
SKRLGIVPTNAKYSLISSTLKELFSAYDLLHLHHMLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEAWRSNTS

Sequences:

>Translated_236_residues
MKCTAETIFHKLSATYIIKEEDFIAYYVWLKTKDCFKVLVATILSQNSTDKSAIKAYLELERKVGVTPEKLSNANLADIE
SALKISGLYRTKAKRLKEISRIILERYNGLIDSLLNTSNARDELLKLEGIGEKTADVVLLTCYGYYGYKVFPVDTHITRV
SKRLGIVPTNAKYSLISSTLKELFSAYDLLHLHHMLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEAWRSNTS
>Mature_236_residues
MKCTAETIFHKLSATYIIKEEDFIAYYVWLKTKDCFKVLVATILSQNSTDKSAIKAYLELERKVGVTPEKLSNANLADIE
SALKISGLYRTKAKRLKEISRIILERYNGLIDSLLNTSNARDELLKLEGIGEKTADVVLLTCYGYYGYKVFPVDTHITRV
SKRLGIVPTNAKYSLISSTLKELFSAYDLLHLHHMLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEAWRSNTS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=186, Percent_Identity=26.3440860215054, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787920, Length=192, Percent_Identity=37.5, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17554540, Length=203, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45550361, Length=186, Percent_Identity=29.0322580645161, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26843; Mature: 26843

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCTAETIFHKLSATYIIKEEDFIAYYVWLKTKDCFKVLVATILSQNSTDKSAIKAYLEL
CCCCHHHHHHHHHHEEEEECCCEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ERKVGVTPEKLSNANLADIESALKISGLYRTKAKRLKEISRIILERYNGLIDSLLNTSNA
HHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RDELLKLEGIGEKTADVVLLTCYGYYGYKVFPVDTHITRVSKRLGIVPTNAKYSLISSTL
HHHHHHHHCCCCHHHHEEEEEEECCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHH
KELFSAYDLLHLHHMLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEAWRSNTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKCTAETIFHKLSATYIIKEEDFIAYYVWLKTKDCFKVLVATILSQNSTDKSAIKAYLEL
CCCCHHHHHHHHHHEEEEECCCEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ERKVGVTPEKLSNANLADIESALKISGLYRTKAKRLKEISRIILERYNGLIDSLLNTSNA
HHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RDELLKLEGIGEKTADVVLLTCYGYYGYKVFPVDTHITRVSKRLGIVPTNAKYSLISSTL
HHHHHHHHCCCCHHHHEEEEEEECCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHH
KELFSAYDLLHLHHMLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEAWRSNTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]