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The map label for this gene is purT [H]

Identifier: 15896998

GI number: 15896998

Start: 24484

End: 25713

Strand: Direct

Name: purT [H]

Synonym: SSO0026

Alternate gene names: 15896998

Gene position: 24484-25713 (Clockwise)

Preceding gene: 15896997

Following gene: 15897000

Centisome position: 0.82

GC content: 37.89

Gene sequence:

>1230_bases
TTGAAGATTTATTATCTAGAGTTAAGTAATTGCCATATGGAAATTGGCACTCCCTTATTTGAAGGTTCGAAGAAAATCCT
TCTACTTGGAAGTGGAGAGTTAGGAAAGGAAATGGCTATTGAGGCACAAAGAATGGGATTAGAAGTTGTTGCTTTAGATA
GATACGATCTAGCTCCTGCCATGCACGTTGCTCACAGAAAGTATGTAGTAGATATGATGAATCCAAACGCTATTAAGGCA
GTAGTGAAAAGGGAAAGACCAGATGCGATTATAGCTGAAATAGAGGCAATTAATACAGACGCATTAATTGAACTTGAAAG
CAACGGCTTTAGAGTGGTTCCTAATGCAAATGCAGTAAAAGCTTGCATGAATAGAATTGAATTAAGAAGATTTGCTGCAG
AAAAACTTAAACTTCCAACTACGAAATACGCATTTGCCGAAAATGAAGAAGAGGTAAAACGAGCTTGTAAGGATATTGGT
TTTCCTTGTCTGATTAAACCGGAGATGAGCTCTAGTGGGCATGGCCATGTATTAGTCAATAAAATCGAGAATGTAGAGGA
AGCTTATAGAGAGTCTATATCTCACGCTAGAGGTAAGAGTAGAAGAGTCATTGTAGAAGAGTTCGTAAAAATAGATACTG
AGCTAACAGTATTAACGTATAGATATCATTCGAATTCTGATAGCATAATAACTAAGACCATTGAACCAATAGAACATAAG
AGACCAAGCTATTATTATGTTGAGTCATGGCATCCTTCTAATGTAAGCCAAGGAGTGAAAGAGACTGCTAGGGGAATCGC
ACAAAAGGTTGCAGAAGAACTAGGAGGTCTAGGAATATATGGTGTTGAAATAATAGTTAGTGGAAATAGAATTCTCTTTA
GCGAAGTAGCGCCCAGACCCCATGATACTGGATTAGTTACATTAGCTAGTAGCGATATAAATGAATTCCAAATTCATGTT
AGAAGTGCAATAGGCTTGCCGACTCCAGAAGTTAAGTTAGTTTCCCCAGCGGCCTCTCATGTGATTTTAGCACAAACAGA
AAATGTTTGGGGACCTAAATTTCTAAACATAGAGAAGGCAATGGAGATTCCGGGCGTACAAGTTAGGTTGTTCGGAAAAC
CTGTTACATACGAGAAGAGAAGAATGGGTGTAGTATTGGCAACTGGAAATAGTGTAGAAGAGGCGTTAGAAAAAGTTAGA
AAGGCATCTTCAATAATATTAGTTAAGTGA

Upstream 100 bases:

>100_bases
GGTATGGCAGTATTTCCATTTACTGGAGAGTGTAGAGTACCTAAAGGTTGGATTTGTATATTTAACACCACAAAGGATCA
GTCTAGATTCTACTCTCTTC

Downstream 100 bases:

>100_bases
ATGTATCTTCTTTATTAGACTTATAGTGTTTCTTATTTTCTCCATAACAAACTGCGACGATTTAGTTGAGAAGGAGTCAT
GAAGAGTTTCATATTCTAAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKIYYLELSNCHMEIGTPLFEGSKKILLLGSGELGKEMAIEAQRMGLEVVALDRYDLAPAMHVAHRKYVVDMMNPNAIKA
VVKRERPDAIIAEIEAINTDALIELESNGFRVVPNANAVKACMNRIELRRFAAEKLKLPTTKYAFAENEEEVKRACKDIG
FPCLIKPEMSSSGHGHVLVNKIENVEEAYRESISHARGKSRRVIVEEFVKIDTELTVLTYRYHSNSDSIITKTIEPIEHK
RPSYYYVESWHPSNVSQGVKETARGIAQKVAEELGGLGIYGVEIIVSGNRILFSEVAPRPHDTGLVTLASSDINEFQIHV
RSAIGLPTPEVKLVSPAASHVILAQTENVWGPKFLNIEKAMEIPGVQVRLFGKPVTYEKRRMGVVLATGNSVEEALEKVR
KASSIILVK

Sequences:

>Translated_409_residues
MKIYYLELSNCHMEIGTPLFEGSKKILLLGSGELGKEMAIEAQRMGLEVVALDRYDLAPAMHVAHRKYVVDMMNPNAIKA
VVKRERPDAIIAEIEAINTDALIELESNGFRVVPNANAVKACMNRIELRRFAAEKLKLPTTKYAFAENEEEVKRACKDIG
FPCLIKPEMSSSGHGHVLVNKIENVEEAYRESISHARGKSRRVIVEEFVKIDTELTVLTYRYHSNSDSIITKTIEPIEHK
RPSYYYVESWHPSNVSQGVKETARGIAQKVAEELGGLGIYGVEIIVSGNRILFSEVAPRPHDTGLVTLASSDINEFQIHV
RSAIGLPTPEVKLVSPAASHVILAQTENVWGPKFLNIEKAMEIPGVQVRLFGKPVTYEKRRMGVVLATGNSVEEALEKVR
KASSIILVK
>Mature_409_residues
MKIYYLELSNCHMEIGTPLFEGSKKILLLGSGELGKEMAIEAQRMGLEVVALDRYDLAPAMHVAHRKYVVDMMNPNAIKA
VVKRERPDAIIAEIEAINTDALIELESNGFRVVPNANAVKACMNRIELRRFAAEKLKLPTTKYAFAENEEEVKRACKDIG
FPCLIKPEMSSSGHGHVLVNKIENVEEAYRESISHARGKSRRVIVEEFVKIDTELTVLTYRYHSNSDSIITKTIEPIEHK
RPSYYYVESWHPSNVSQGVKETARGIAQKVAEELGGLGIYGVEIIVSGNRILFSEVAPRPHDTGLVTLASSDINEFQIHV
RSAIGLPTPEVKLVSPAASHVILAQTENVWGPKFLNIEKAMEIPGVQVRLFGKPVTYEKRRMGVVLATGNSVEEALEKVR
KASSIILVK

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=397, Percent_Identity=47.103274559194, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324702, Length=389, Percent_Identity=23.3933161953728, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 45539; Mature: 45539

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYYLELSNCHMEIGTPLFEGSKKILLLGSGELGKEMAIEAQRMGLEVVALDRYDLAPA
CEEEEEEECCCEEEECCCEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHH
MHVAHRKYVVDMMNPNAIKAVVKRERPDAIIAEIEAINTDALIELESNGFRVVPNANAVK
HHHHHHHEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCEEEECCCHHHH
ACMNRIELRRFAAEKLKLPTTKYAFAENEEEVKRACKDIGFPCLIKPEMSSSGHGHVLVN
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEH
KIENVEEAYRESISHARGKSRRVIVEEFVKIDTELTVLTYRYHSNSDSIITKTIEPIEHK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCEEEEECCHHHHC
RPSYYYVESWHPSNVSQGVKETARGIAQKVAEELGGLGIYGVEIIVSGNRILFSEVAPRP
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCCCC
HDTGLVTLASSDINEFQIHVRSAIGLPTPEVKLVSPAASHVILAQTENVWGPKFLNIEKA
CCCCEEEEECCCCCEEEEEEEHHCCCCCCCEEEECCCCCEEEEEECCCCCCCEEECHHHH
MEIPGVQVRLFGKPVTYEKRRMGVVLATGNSVEEALEKVRKASSIILVK
HCCCCEEEEEECCCCCCCCHHEEEEEEECCHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKIYYLELSNCHMEIGTPLFEGSKKILLLGSGELGKEMAIEAQRMGLEVVALDRYDLAPA
CEEEEEEECCCEEEECCCEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHH
MHVAHRKYVVDMMNPNAIKAVVKRERPDAIIAEIEAINTDALIELESNGFRVVPNANAVK
HHHHHHHEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCEEEECCCHHHH
ACMNRIELRRFAAEKLKLPTTKYAFAENEEEVKRACKDIGFPCLIKPEMSSSGHGHVLVN
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEH
KIENVEEAYRESISHARGKSRRVIVEEFVKIDTELTVLTYRYHSNSDSIITKTIEPIEHK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCEEEEECCHHHHC
RPSYYYVESWHPSNVSQGVKETARGIAQKVAEELGGLGIYGVEIIVSGNRILFSEVAPRP
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCCCC
HDTGLVTLASSDINEFQIHVRSAIGLPTPEVKLVSPAASHVILAQTENVWGPKFLNIEKA
CCCCEEEEECCCCCEEEEEEEHHCCCCCCCEEEECCCCCEEEEEECCCCCCCEEECHHHH
MEIPGVQVRLFGKPVTYEKRRMGVVLATGNSVEEALEKVRKASSIILVK
HCCCCEEEEEECCCCCCCCHHEEEEEEECCHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]