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The map label for this gene is phoB

Identifier: 15928213

GI number: 15928213

Start: 2715011

End: 2716435

Strand: Direct

Name: phoB

Synonym: SA2420

Alternate gene names: 15928213

Gene position: 2715011-2716435 (Clockwise)

Preceding gene: 15928200

Following gene: 15928225

Centisome position: 96.45

GC content: 35.16

Gene sequence:

>1425_bases
ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACAGTTTTCAGTTCTGTCACACC
TGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGTTTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCT
TTTTAGTTGGCGATGGCATGGGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATTCGCATGCCAAAGAAATG
ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCAAAACAGAATATTACTGATTC
TGCAGCTGGCGGAACAGCCTTTAGTTCTGGCCATAAGACATATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAG
TAAAGACAGTGCTTGAACGTGCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAA
CGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAAT
TCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGATTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGATATGCAACAAAGTGCACTTAG
TAAGCTTGAGCGTAATAAAAAAGGTTTCTTTTTAATGGTCGAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATA
TTACAGGCGTTATGTCTGAAATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAACATAAAGAT
ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAAGGATACGAATGGAATCCTCA
ACCGATTAAATCGATGAAACACTCTGGATCATATATGACAGAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTA
ATGAAGGTTATGGCTTTGAATTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGTTGCAAAAATTACTT
AAAAAAGAAAAAGACGAAAAATCTGCAGATGTAGAAAAACAAACAAAAGCCTTACAACACGCAATTCAGAAGCCAGTAAA
TGATAAATCTTATACAGGGTGGACTTCCGATGGACATACAGGCGAGGATGTTAATACCTACGCTTACGGACCTAGATCAG
AATCATTTAGTGGTAATATCGAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA

Upstream 100 bases:

>100_bases
CTAAATCACTCTGTTTACATATTTTTAACTAAAACTACATAATTGCTCAAAATTCCTCTTTTACACTGAAGACATAGTCA
TATACTCAGGAGGATGTATT

Downstream 100 bases:

>100_bases
AGCATAAAAAAATTCCCGAGCACTATAACAAGGTGTTCGGGAATTTTTTATGGTTTAATTTAGTACTCAATGTTAGGTGC
TTGGTTAACACGTTCTTGGT

Product: alkaline phosphatase III precursor

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEM
TPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLF
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKD
TLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLL
KKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

Sequences:

>Translated_474_residues
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEM
TPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLF
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKD
TLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLL
KKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK
>Mature_474_residues
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEM
TPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLF
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKD
TLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLL
KKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=476, Percent_Identity=28.1512605042017, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI116734717, Length=338, Percent_Identity=30.7692307692308, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI94721246, Length=482, Percent_Identity=27.5933609958506, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI157266296, Length=482, Percent_Identity=27.3858921161826, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI294660770, Length=329, Percent_Identity=29.7872340425532, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI294660772, Length=302, Percent_Identity=28.8079470198675, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI48994877, Length=349, Percent_Identity=36.9627507163324, Blast_Score=177, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320689, Length=526, Percent_Identity=32.8897338403042, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI18859923, Length=492, Percent_Identity=29.0650406504065, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651554, Length=494, Percent_Identity=29.7570850202429, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355149, Length=476, Percent_Identity=29.4117647058824, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19921912, Length=473, Percent_Identity=28.1183932346723, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21355981, Length=473, Percent_Identity=27.4841437632135, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24651556, Length=481, Percent_Identity=29.3139293139293, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI85725200, Length=339, Percent_Identity=30.0884955752212, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI85815833, Length=339, Percent_Identity=30.0884955752212, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21355151, Length=337, Percent_Identity=29.9703264094956, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24585213, Length=348, Percent_Identity=30.4597701149425, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21358067, Length=475, Percent_Identity=25.8947368421053, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24657835, Length=478, Percent_Identity=26.5690376569038, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24657842, Length=339, Percent_Identity=31.2684365781711, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24657827, Length=467, Percent_Identity=27.1948608137045, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51922; Mature: 51922

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGM
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCEECCCCCCCCEEEEEEECCC
GPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKT
CCCHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCHHHHHHHCCCCCHHHHHHH
FYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLF
HHHHHCCCCEEEEEEECCCHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHEEEEE
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSE
ECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHH
MEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHCCCHHH
EKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLLKKEKDEKSADVEKQTKALQH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
AIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK
HHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGM
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCEECCCCCCCCEEEEEEECCC
GPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKT
CCCHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCHHHHHHHCCCCCHHHHHHH
FYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLF
HHHHHCCCCEEEEEEECCCHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHEEEEE
ADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSE
ECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHH
MEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHCCCHHH
EKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLLKKEKDEKSADVEKQTKALQH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
AIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK
HHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]