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The map label for this gene is gudB

Identifier: 15926547

GI number: 15926547

Start: 923189

End: 924433

Strand: Direct

Name: gudB

Synonym: SA0819

Alternate gene names: 15926547

Gene position: 923189-924433 (Clockwise)

Preceding gene: 15926546

Following gene: 15926551

Centisome position: 32.8

GC content: 36.87

Gene sequence:

>1245_bases
ATGACTGAGAACAATAATTTAGTAACTTCTACTCAAGGAATTATTAAAGAAGCATTGCATAAATTGGGATTTGACGAAGG
AATGTACGATTTAATTAAAGAACCTTTAAGAATGTTACAAGTGCGTATCCCTGTACGAATGGATGATGGCACAGTTAAAA
CATTCACAGGTTACCGTGCGCAACATAATGATGCTGTTGGACCAACAAAAGGGGGCGTGCGTTTCCACCCAGATGTTGAT
GAAGAAGAAGTAAAAGCATTATCAATGTGGATGACTTTGAAATGTGGCATTGTAAACTTACCATACGGTGGTGGTAAGGG
TGGTATCGTTTGTGATCCACGTCAAATGAGCATTCATGAAGTTGAACGTTTATCACGCGGATATGTAAGAGCAATTTCAC
AATTCGTAGGTCCGAACAAAGATATTCCAGCACCAGATGTATTTACAAACTCACAAATTATGGCTTGGATGATGGATGAA
TATAGTGCATTAGATAAATTTAATTCACCAGGTTTCATCACAGGTAAACCAATTGTATTGGGTGGTTCTCATGGACGCGA
CAGATCAACTGCACTAGGTGTAGTTATTGCAATTGAACAAGCTGCAAAACGTCGTAATATGCAAATTGAAGGTGCCAAGG
TTGTTATTCAAGGTTTCGGTAATGCCGGAAGTTTCTTAGCTAAATTCTTATATGATTTAGGTGCAAAAATTGTAGGTATC
TCTGATGCTTACGGTGCATTACACGATCCAAATGGCTTAGATATAGATTATTTATTAGACCGTCGTGATAGTTTTGGTAC
GGTAACAAATTTATTTGAAGAAACAATCTCAAATAAAGAATTGTTTGAATTAGATTGTGACATTTTAGTACCAGCGGCTA
TTTCAAACCAAATTACAGAAGACAATGCACATGATATTAAAGCTAGTATCGTTGTTGAAGCTGCTAATGGACCTACAACA
CCAGAAGCAACACGTATTTTAACTGAACGTGGTATATTATTAGTTCCAGACGTATTAGCAAGTGCTGGTGGTGTAACGGT
TTCTTACTTCGAATGGGTACAAAATAATCAAGGTTATTATTGGTCTGAAGAAGAAGTAAATGAAAAGCTACGTGAAAAAT
TAGAAGCGGCATTTGACACGATTTACGAATTGTCTCAAAATCGAAAAATAGATATGAGACTTGCAGCATATATCATAGGT
ATTAAACGTACAGCAGAAGCAGCTAGATATCGTGGTTGGGCATAA

Upstream 100 bases:

>100_bases
AGTTGTAACAATCAGTTTGTAAGGGCTTTAAAAAGGAATACGTTATGTTACAATATGTTTGAAAGCGAAATCATTAGGTA
AAGAAGAGGCGAAAAGGATC

Downstream 100 bases:

>100_bases
TTAATTATCATATGTGATTTAACGAGCTTGGGACAGAAAACAAAGCCCTAAGCTCGTTAATTTTATTTTAGCAGCAGTTG
ACTGTAAAACAATGCCCGTG

Product: NAD-specific glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVD
EEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGI
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTT
PEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIG
IKRTAEAARYRGWA

Sequences:

>Translated_414_residues
MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVD
EEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGI
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTT
PEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIG
IKRTAEAARYRGWA
>Mature_413_residues
TENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDE
EEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEY
SALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGIS
DAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTP
EATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGI
KRTAEAARYRGWA

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=342, Percent_Identity=39.766081871345, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI4885281, Length=342, Percent_Identity=39.4736842105263, Blast_Score=268, Evalue=7e-72,
Organism=Escherichia coli, GI1788059, Length=402, Percent_Identity=30.8457711442786, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=346, Percent_Identity=40.7514450867052, Blast_Score=262, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6319256, Length=386, Percent_Identity=29.0155440414508, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324951, Length=399, Percent_Identity=27.5689223057644, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553475, Length=382, Percent_Identity=39.0052356020942, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI45549226, Length=346, Percent_Identity=40.1734104046243, Blast_Score=267, Evalue=9e-72,
Organism=Drosophila melanogaster, GI24649283, Length=330, Percent_Identity=40.6060606060606, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649460, Length=281, Percent_Identity=39.5017793594306, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE2_STAAC (Q5HHC7)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185830.1
- ProteinModelPortal:   Q5HHC7
- SMR:   Q5HHC7
- STRING:   Q5HHC7
- EnsemblBacteria:   EBSTAT00000009499
- GeneID:   3237698
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL0961
- TIGR:   SACOL0961
- eggNOG:   COG0334
- GeneTree:   EBGT00050000024253
- HOGENOM:   HBG590661
- OMA:   RIEAHYL
- ProtClustDB:   CLSK885029
- BioCyc:   SAUR93062:SACOL0961-MONOMER
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.2

Molecular weight: Translated: 45761; Mature: 45629

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRA
CCCCCCEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCEEECCCCEEEEECCCC
QHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHE
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHEEECCEEECCCCCCCCCEEECCCCCCHHH
VERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEYSALDKFNSPGFITGKPIVL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCEEE
GGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGI
ECCCCCCCHHHEEEEEEEHHHHHHCCCEECCEEEEEEECCCHHHHHHHHHHHCCCEEEEE
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITE
CCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEECHHHCCCCCC
DNAHDIKASIVVEAANGPTTPEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYY
CCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEHHHHHCCCCEE
WSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGIKRTAEAARYRGWA
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRA
CCCCCEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCEEECCCCEEEEECCCC
QHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHE
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHEEECCEEECCCCCCCCCEEECCCCCCHHH
VERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEYSALDKFNSPGFITGKPIVL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCEEE
GGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGI
ECCCCCCCHHHEEEEEEEHHHHHHCCCEECCEEEEEEECCCHHHHHHHHHHHCCCEEEEE
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITE
CCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEECHHHCCCCCC
DNAHDIKASIVVEAANGPTTPEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYY
CCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEHHHHHCCCCEE
WSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGIKRTAEAARYRGWA
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA