Click here to switch to the map view.

The map label for this gene is nrdE

Identifier: 15926408

GI number: 15926408

Start: 783420

End: 785576

Strand: Direct

Name: nrdE

Synonym: SA0686

Alternate gene names: 15926408

Gene position: 783420-785576 (Clockwise)

Preceding gene: 15926407

Following gene: 15926409

Centisome position: 27.83

GC content: 32.64

Gene sequence:

>2157_bases
ATGGAAAAAACAAAGACGTTATTGAATTTAAGAACAAGGTGGGTAATTTTAATGAAAACCATGGAAGAGAAAAAGTACAA
TCATATTGAATTAAATAATGAGGTCACTAAACGAAGAGAAGATGGATTCTTTAGTTTAGAAAAAGACCAAGAAGCTTTAG
TAGCTTATTTAGAAGAAGTAAAAGACAAAACAATCTTCTTCGATACTGAAATCGAGCGTTTACGTTATTTAGTAGACAAC
GATTTTTATTTCAATGTGTTTGATATTTATAGTGAAGCGGATCTAATTGAAATCACTGATTATGCAAAATCAATCTCGTT
TAATTTTGCAAGTTATATGTCAGCTAGTAAATTTTTCAAAGATTACGCTTTGAAAACAAATGATAAAAGTCAATACTTAG
AAGACTATAATCAACACGTTGCCATTGTTGCTTTATACCTAGCAAATGGTAATAAAGCACAAGCTAAACAATTTATTTCT
GCTATGGTTGAACAAAGATATCAACCAGCGACACCAACATTTTTAAACGCAGGCCGTGCGCGTCGTGGTGAGCTAGTGTC
ATGTTTCTTATTAGAAGTGGATGACAGCTTAAATTCAATTAACTTTATTGATTCAACTGCAAAACAATTAAGTAAAATTG
GGGGCGGCGTTGCAATTAACTTATCTAAATTGCGTGCACGTGGTGAAGCAATTAAAGGAATTAAAGGCGTAGCGAAAGGC
GTTTTACCTATTGCTAAGTCACTTGAAGGTGGCTTTAGCTATGCAGATCAACTTGGTCAACGCCCTGGTGCTGGTGCTGT
GTACTTAAATATCTTCCATTATGATGTAGAAGAATTTTTAGATACTAAAAAAGTAAATGCGGATGAAGATTTACGTTTAT
CTACAATATCAACTGGTTTAATTGTTCCATCTAAATTCTTCGATTTAGCTAAAGAAGGTAAGGACTTTTATATGTTTGCA
CCTCATACAGTTAAAGAAGAATATGGTGTGACATTAGACGATATCGATTTAGAAAAATATTATGATGACATGGTTGCAAA
CCCAAATGTTGAGAAAAAGAAAAAGAATGCGCGTGAAATGTTGAATTTAATTGCGCAAACACAATTACAATCAGGTTATC
CATATTTAATGTTTAAAGATAATGCTAACAGAGTGCATCCGAATTCAAACATTGGACAAATTAAAATGAGTAACTTATGT
ACGGAAATTTTCCAACTACAAGAAACTTCAATTATTAATGACTATGGTATTGAAGACGAAATTAAACGTGATATTTCTTG
TAACTTGGGCTCATTAAATATTGTTAATGTAATGGAAAGCGGAAAATTCAGAGATTCAGTTCACTCTGGTATGGACGCAT
TAACTGTTGTGAGTGATGTAGCAAATATTCAAAATGCACCAGGAGTTAGAAAAGCTAACAGTGAATTACATTCAGTTGGT
CTTGGTGTGATGAATTTACACGGTTACCTAGCAAAAAATAAAATTGGTTATGAGTCAGAAGAAGCAAAAGATTTTGCAAA
TATCTTCTTTATGATGATGAATTTCTACTCAATCGAACGTTCAATGGAAATCGCTAAAGAGCGTGGTATCAAATATCAAG
ACTTTGAAAAGTCTGATTATGCTAATGGCAAATATTTCGAGTTCTATACAACTCAAGAATTTGAACCTCAATTCGAAAAA
GTACGTGAATTATTCGATGGTATGGCTATTCCTACTTCTGAGGATTGGAAGAAACTACAACAAGATGTTGAACAATATGG
TTTATATCATGCATATAGATTAGCAATTGCTCCAACACAAAGTATTTCTTATGTTCAAAATGCAACAAGTTCTGTAATGC
CAATCGTTGACCAAATTGAACGTCGTACTTATGGTAATGCGGAAACATTTTACCCTATGCCATTCTTATCACCACAAACA
ATGTGGTACTACAAATCAGCATTCAATACTGATCAGATGAAATTAATCGATTTAATTGCGACAATTCAAACGCATATTGA
CCAAGGTATCTCAACGATCCTTTATGTTAATTCTGAAATTTCTACACGTGAGTTAGCAAGATTATATGTATATGCGCACT
ATAAAGGATTAAAATCACTTTACTATACTAGAAATAAATTATTAAGTGTAGAAGAATGTACAAGTTGTTCTATCTAA

Upstream 100 bases:

>100_bases
GCAGCTAGCGGTAATCGAAATTGGGGACTAAATTTCGCAAAAGCGGGTCGCACGATATCAGAAGAGTATAATGTCCCTTT
ATTAATGAAGTTTGAGTTAC

Downstream 100 bases:

>100_bases
CAATTAAATGTTGAAAATGACAAACAGCTAATCATCTGGTCTGAATTAGCAGATGATTAGACTGCTATGTCTGTATTTGT
CAATTATTGAGTAACATTAC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MEKTKTLLNLRTRWVILMKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDN
DFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFIS
AMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKG
VLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFA
PHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLC
TEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVG
LGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEK
VRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQT
MWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI

Sequences:

>Translated_718_residues
MEKTKTLLNLRTRWVILMKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDN
DFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFIS
AMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKG
VLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFA
PHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLC
TEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVG
LGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEK
VRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQT
MWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
>Mature_718_residues
MEKTKTLLNLRTRWVILMKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDN
DFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFIS
AMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKG
VLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFA
PHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLC
TEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVG
LGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEK
VRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQT
MWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=655, Percent_Identity=26.5648854961832, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1789030, Length=687, Percent_Identity=41.6302765647744, Blast_Score=570, Evalue=1e-163,
Organism=Escherichia coli, GI2367133, Length=572, Percent_Identity=23.9510489510489, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=642, Percent_Identity=26.01246105919, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=649, Percent_Identity=25.7318952234206, Blast_Score=186, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=646, Percent_Identity=24.3034055727554, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17352481, Length=650, Percent_Identity=26.7692307692308, Blast_Score=189, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 82214; Mature: 82214

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTKTLLNLRTRWVILMKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEV
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCCEEECCHHHHHHHHHHHH
KDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFFK
CCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
DYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNAGRA
HHHCCCCCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCC
RRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKG
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
VLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGL
HHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCCC
IVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREM
CCCHHHHHHHHCCCCEEEECCCHHHHHHCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
LNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGIEDE
HHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHH
IKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVG
HHHCCCCCCCCEEEEEHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHC
LGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDY
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQ
CCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEEEEECCCH
SISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLIDLIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHH
TIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MEKTKTLLNLRTRWVILMKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEV
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCCEEECCHHHHHHHHHHHH
KDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFFK
CCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
DYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNAGRA
HHHCCCCCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCC
RRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKG
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
VLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGL
HHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCCC
IVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREM
CCCHHHHHHHHCCCCEEEECCCHHHHHHCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHH
LNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGIEDE
HHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHH
IKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVG
HHHCCCCCCCCEEEEEHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHC
LGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDY
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQ
CCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEEEEECCCH
SISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLIDLIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHH
TIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]