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The map label for this gene is pbpE [H]

Identifier: 16127719

GI number: 16127719

Start: 3731080

End: 3732807

Strand: Direct

Name: pbpE [H]

Synonym: CC_3489

Alternate gene names: 16127719

Gene position: 3731080-3732807 (Clockwise)

Preceding gene: 16127718

Following gene: 16127728

Centisome position: 92.88

GC content: 69.27

Gene sequence:

>1728_bases
ATGCGCCGTCTTTTTGTCGCTCTAGCCGCAACCGCCCTCGTTGGCGTAGCCCCCTTGGGCGCCTTCGCCCAGACGGCGGC
GGGGCCGGCGAAGCCTGCCACGGTTTCGACCGATCGGTTGTCGGACGCGGCTATCCGCCAGATCCTGGCCCAGCGGGTCG
ACGTCGATCATCAGAGCCGGGGTATGGTGGTCGGGGTGATCGACGCCCGAGGCCGGCGCGTGATCGCCCACGGCCTCATG
GGCGCCGGTGGCAAGCCGGTCGACGGTCAGACCCTGTTCGAGATCGGCTCGGTCACCAAGGCGCTGACCAGCCTCTTGCT
GGCCGACATGGTCCGGCGCGGTGAGGTCAGGCTGGAGGATCCGGTGGCTGACCATCTGCCGCCGGGAACGGTCGTGCCGA
CCCGGAACGGCAAGGCCATCACCCTGATCGACCTCGCCACCCACACCTCGGGCCTGCCGCGCCTGCCGACCAACATGGCG
ATGAAGGATCCGCTCAACCCCTACGCCGACTATACCGAGGCTCAGCTGGATGCGTTCCTGCGCGACTATGTCCTGACCCG
CGACATCGGCGGGATCTACGAATACTCCAACCTTGGGGTGGGATTGCTGGGTCGGGCCCTGGCCTATCGCGCGGGCGGCG
ACTACGAGACCGTATTGCGCCAGCGTGTGCTGACGCCGCTGGGCATGAGCGACACCGCCATCGTCCTGTCACCGGCCCAG
GCCGCGCGCTTCTCCAGCGGCCATAACGCCGCGCTGGAGGCCACGACGCATTGGGACCTGCCCAGCCTGGCCGGCGCGGG
CGCCCTGCGCTCGACCGCCGACGATCTGCTGAAGCTGACGGCTGCGGAGCTTGGCCTTGTCGACACGCCGCTGAAGGCCG
CTATGGCCGACCAGCTGGTTCCACGTCGCCCCGTCGGCGGCGGCGTGGAGGTCGCGCTAGGCTGGCACGTCTGGTCAACA
CCCGAGGGCGGCATCGTCACCCATAGCGGCGGCACCATGGGCTTCCAAAGCTTCGTCGGTTTCAATCGTAAGACCGGACT
GGGTGTCGTGGTGCTGAGCAACACGGCCGGCGTCATGGGCGTGGACGATATCGGGCTGCACCTGATGACCGGGCAGCCAC
TGAAGACCCCGCCCAAGACGCGCGTGGCGGTTCCCCTCGCCCCCGCCGCCTTCGACAAGCTGGTCGGCCGCTACGCCATG
GCGCCGGGGGCGGTGATGACGATCCGCCGCGATGGTGAGCGCATGCTGGGCCAGCTGACCGGGCAGCCGACGGTCGAGTT
GTTCGCCGAAAGCCCGACAACCTTCTTTCTCAAGGTGGTCGACGCCCAACTGACCTTCGCCGTCGACGCCGAGGGGCGCG
GGACGGCGGTGACGCTGCACCAGAACGGCCAGAACACGACGGCCCCGCGCTTGGCCGAAGGGGCTGAGGTGCCCGCGCCG
CCGCGCCCGGCCAAGGTGGCGGCACTGTCCGTCGCCGAGCTGGACGCCCTGACGGGCCGATACGCCCTGGCTCCCGGATT
CGTCGTCACCGTGACCCGCAAGGGCCAGAGCCTGTTCGCCCAGCTCACCGGCCAGCCGGAGTTCGAGGTGTTTCCCGAAA
GCGCGACCCGCGTCTTCTGGACCGTCGTTCCCGCCGCCGCCAGTTTTACGCTGGGTCCGGATGGGAAGGCCGTGAGCCTG
ACCTTGCATCAGGGCGGCCGCGACATGCCCGCGCCGCGACAGCCTTAG

Upstream 100 bases:

>100_bases
GGACGCCGCGCCCCAGCAGTCGCCTCAGGGCGTCTAGCGCCGCTTGCTATCTGGGGCGGCGTCGACAAGACTGCTCAACC
TCTGAGGCGGGAGGGCCCTG

Downstream 100 bases:

>100_bases
ACGCCGCCTCGGTCAGATGGAAACATCCAACCTAGATAGAAAGACGTAAAATCATACGCTTGGCGCGCTACGGGCCTCAG
CCCTACGCCTTCTTCAGCCA

Product: penicillin-binding protein AmpH

Products: NA

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MRRLFVALAATALVGVAPLGAFAQTAAGPAKPATVSTDRLSDAAIRQILAQRVDVDHQSRGMVVGVIDARGRRVIAHGLM
GAGGKPVDGQTLFEIGSVTKALTSLLLADMVRRGEVRLEDPVADHLPPGTVVPTRNGKAITLIDLATHTSGLPRLPTNMA
MKDPLNPYADYTEAQLDAFLRDYVLTRDIGGIYEYSNLGVGLLGRALAYRAGGDYETVLRQRVLTPLGMSDTAIVLSPAQ
AARFSSGHNAALEATTHWDLPSLAGAGALRSTADDLLKLTAAELGLVDTPLKAAMADQLVPRRPVGGGVEVALGWHVWST
PEGGIVTHSGGTMGFQSFVGFNRKTGLGVVVLSNTAGVMGVDDIGLHLMTGQPLKTPPKTRVAVPLAPAAFDKLVGRYAM
APGAVMTIRRDGERMLGQLTGQPTVELFAESPTTFFLKVVDAQLTFAVDAEGRGTAVTLHQNGQNTTAPRLAEGAEVPAP
PRPAKVAALSVAELDALTGRYALAPGFVVTVTRKGQSLFAQLTGQPEFEVFPESATRVFWTVVPAAASFTLGPDGKAVSL
TLHQGGRDMPAPRQP

Sequences:

>Translated_575_residues
MRRLFVALAATALVGVAPLGAFAQTAAGPAKPATVSTDRLSDAAIRQILAQRVDVDHQSRGMVVGVIDARGRRVIAHGLM
GAGGKPVDGQTLFEIGSVTKALTSLLLADMVRRGEVRLEDPVADHLPPGTVVPTRNGKAITLIDLATHTSGLPRLPTNMA
MKDPLNPYADYTEAQLDAFLRDYVLTRDIGGIYEYSNLGVGLLGRALAYRAGGDYETVLRQRVLTPLGMSDTAIVLSPAQ
AARFSSGHNAALEATTHWDLPSLAGAGALRSTADDLLKLTAAELGLVDTPLKAAMADQLVPRRPVGGGVEVALGWHVWST
PEGGIVTHSGGTMGFQSFVGFNRKTGLGVVVLSNTAGVMGVDDIGLHLMTGQPLKTPPKTRVAVPLAPAAFDKLVGRYAM
APGAVMTIRRDGERMLGQLTGQPTVELFAESPTTFFLKVVDAQLTFAVDAEGRGTAVTLHQNGQNTTAPRLAEGAEVPAP
PRPAKVAALSVAELDALTGRYALAPGFVVTVTRKGQSLFAQLTGQPEFEVFPESATRVFWTVVPAAASFTLGPDGKAVSL
TLHQGGRDMPAPRQP
>Mature_575_residues
MRRLFVALAATALVGVAPLGAFAQTAAGPAKPATVSTDRLSDAAIRQILAQRVDVDHQSRGMVVGVIDARGRRVIAHGLM
GAGGKPVDGQTLFEIGSVTKALTSLLLADMVRRGEVRLEDPVADHLPPGTVVPTRNGKAITLIDLATHTSGLPRLPTNMA
MKDPLNPYADYTEAQLDAFLRDYVLTRDIGGIYEYSNLGVGLLGRALAYRAGGDYETVLRQRVLTPLGMSDTAIVLSPAQ
AARFSSGHNAALEATTHWDLPSLAGAGALRSTADDLLKLTAAELGLVDTPLKAAMADQLVPRRPVGGGVEVALGWHVWST
PEGGIVTHSGGTMGFQSFVGFNRKTGLGVVVLSNTAGVMGVDDIGLHLMTGQPLKTPPKTRVAVPLAPAAFDKLVGRYAM
APGAVMTIRRDGERMLGQLTGQPTVELFAESPTTFFLKVVDAQLTFAVDAEGRGTAVTLHQNGQNTTAPRLAEGAEVPAP
PRPAKVAALSVAELDALTGRYALAPGFVVTVTRKGQSLFAQLTGQPEFEVFPESATRVFWTVVPAAASFTLGPDGKAVSL
TLHQGGRDMPAPRQP

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI239747261, Length=227, Percent_Identity=30.3964757709251, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI239752721, Length=227, Percent_Identity=30.3964757709251, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI239741085, Length=227, Percent_Identity=30.3964757709251, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786574, Length=341, Percent_Identity=29.9120234604106, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790593, Length=331, Percent_Identity=28.3987915407855, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: NA

Molecular weight: Translated: 60304; Mature: 60304

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLFVALAATALVGVAPLGAFAQTAAGPAKPATVSTDRLSDAAIRQILAQRVDVDHQSR
CCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GMVVGVIDARGRRVIAHGLMGAGGKPVDGQTLFEIGSVTKALTSLLLADMVRRGEVRLED
CEEEEEECCCCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
PVADHLPPGTVVPTRNGKAITLIDLATHTSGLPRLPTNMAMKDPLNPYADYTEAQLDAFL
CHHHCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHCHHHHHHHHHH
RDYVLTRDIGGIYEYSNLGVGLLGRALAYRAGGDYETVLRQRVLTPLGMSDTAIVLSPAQ
HHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCHH
AARFSSGHNAALEATTHWDLPSLAGAGALRSTADDLLKLTAAELGLVDTPLKAAMADQLV
HHHCCCCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PRRPVGGGVEVALGWHVWSTPEGGIVTHSGGTMGFQSFVGFNRKTGLGVVVLSNTAGVMG
CCCCCCCCEEEEEEEEEECCCCCCEEEECCCCCCHHHHHCCCCCCCCEEEEEECCCCEEE
VDDIGLHLMTGQPLKTPPKTRVAVPLAPAAFDKLVGRYAMAPGAVMTIRRDGERMLGQLT
ECCCCEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHC
GQPTVELFAESPTTFFLKVVDAQLTFAVDAEGRGTAVTLHQNGQNTTAPRLAEGAEVPAP
CCCEEEEEECCCCEEEEEEEECEEEEEEECCCCCCEEEEEECCCCCCCCHHHCCCCCCCC
PRPAKVAALSVAELDALTGRYALAPGFVVTVTRKGQSLFAQLTGQPEFEVFPESATRVFW
CCCCCEEEEEHHHHHHHCCCEEECCCEEEEEEECCCEEHHHHCCCCCEEECCCCCCEEEE
TVVPAAASFTLGPDGKAVSLTLHQGGRDMPAPRQP
EEECCCEEEEECCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRRLFVALAATALVGVAPLGAFAQTAAGPAKPATVSTDRLSDAAIRQILAQRVDVDHQSR
CCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GMVVGVIDARGRRVIAHGLMGAGGKPVDGQTLFEIGSVTKALTSLLLADMVRRGEVRLED
CEEEEEECCCCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
PVADHLPPGTVVPTRNGKAITLIDLATHTSGLPRLPTNMAMKDPLNPYADYTEAQLDAFL
CHHHCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHCHHHHHHHHHH
RDYVLTRDIGGIYEYSNLGVGLLGRALAYRAGGDYETVLRQRVLTPLGMSDTAIVLSPAQ
HHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCHH
AARFSSGHNAALEATTHWDLPSLAGAGALRSTADDLLKLTAAELGLVDTPLKAAMADQLV
HHHCCCCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PRRPVGGGVEVALGWHVWSTPEGGIVTHSGGTMGFQSFVGFNRKTGLGVVVLSNTAGVMG
CCCCCCCCEEEEEEEEEECCCCCCEEEECCCCCCHHHHHCCCCCCCCEEEEEECCCCEEE
VDDIGLHLMTGQPLKTPPKTRVAVPLAPAAFDKLVGRYAMAPGAVMTIRRDGERMLGQLT
ECCCCEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHC
GQPTVELFAESPTTFFLKVVDAQLTFAVDAEGRGTAVTLHQNGQNTTAPRLAEGAEVPAP
CCCEEEEEECCCCEEEEEEEECEEEEEEECCCCCCEEEEEECCCCCCCCHHHCCCCCCCC
PRPAKVAALSVAELDALTGRYALAPGFVVTVTRKGQSLFAQLTGQPEFEVFPESATRVFW
CCCCCEEEEEHHHHHHHCCCEEECCCEEEEEEECCCEEHHHHCCCCCEEECCCCCCEEEE
TVVPAAASFTLGPDGKAVSLTLHQGGRDMPAPRQP
EEECCCEEEEECCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]