Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 16127515

GI number: 16127515

Start: 3539584

End: 3540297

Strand: Direct

Name: dosC [H]

Synonym: CC_3285

Alternate gene names: 16127515

Gene position: 3539584-3540297 (Clockwise)

Preceding gene: 16127512

Following gene: 16127516

Centisome position: 88.12

GC content: 67.23

Gene sequence:

>714_bases
ATGAAAATCTCAGGCGCCCGGACCGAACCCTTCGCCGCCATCCGCAAGCGTGCGTTGGCCCGCGCCGGCGCGCAGGTCGC
CGCGCGCGAAGTGTCCGCGCCTGATAGCGCCGCCTTCCTGGGCCTGACCGAGGCCGACCTGACGCCCAAGGTCGTTGAAG
CCCTTCAGACGCTCATGGGCGAGATCGAGGACCTGCGAAACGAGGTCTCGGTGCTGAAGCTGCGGCTGAACGAGGCGCAG
GGACTGGCGGATATGGACGTCCTGACGCCGGTTCTGAACCGGCGGGCGTTCTTGCGCGAGCTGAAGCGCGTCGCCGCCTT
CGCCCAACGCTACGGTTCGCCGGCCAGCGTGGTCTTCTTCGACCTGGACGGCTTCAAGAGCGTCAACGACCGCTTCGGCC
ACGCCGCCGGCGACGAGGCCCTCAAGGCCGTCGCCAAGCGGTTGCAGGCCAATGTGCGCGAAAGCGACATCGTCGGCCGC
ATGGGTGGTGACGAGTTCGCCGTGCTGCTGGCGCAGGCTGACAAGGAAACGGCCTTGGCCAAGGCTCAGAGTCTGGCCGA
AGCTGTCCGAGCAGAACCCGTGGAGTTCGGCGAATGGTCGGCTCCGCTGCACATCTCGTTCGGCGTGCGCGAAATTGAGC
CTGGGGCCGATCCGGAAGCAGCCCTCGCCGAAGCGGATGCGGCGATGTTCCTGCGCAAGCGCAGGAGCGCTTGA

Upstream 100 bases:

>100_bases
CAGGCATCCATCCCCTGCGACTCTTCGGCGCCCCGATTGCGCCGAACGCCGTTCCCGTTAACCATGGTAAAGCTGTGCAA
TTCGATTCCCCCGTAAGGCC

Downstream 100 bases:

>100_bases
TCGCCCCAGGCCGCCACCCATGGCGCCCAACCATGGGCACGACAACCGCGAGTTATGCGACCTTATCGCACGGGGACTCA
TTCTGCGTCGCCGCGCTAAT

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKISGARTEPFAAIRKRALARAGAQVAAREVSAPDSAAFLGLTEADLTPKVVEALQTLMGEIEDLRNEVSVLKLRLNEAQ
GLADMDVLTPVLNRRAFLRELKRVAAFAQRYGSPASVVFFDLDGFKSVNDRFGHAAGDEALKAVAKRLQANVRESDIVGR
MGGDEFAVLLAQADKETALAKAQSLAEAVRAEPVEFGEWSAPLHISFGVREIEPGADPEAALAEADAAMFLRKRRSA

Sequences:

>Translated_237_residues
MKISGARTEPFAAIRKRALARAGAQVAAREVSAPDSAAFLGLTEADLTPKVVEALQTLMGEIEDLRNEVSVLKLRLNEAQ
GLADMDVLTPVLNRRAFLRELKRVAAFAQRYGSPASVVFFDLDGFKSVNDRFGHAAGDEALKAVAKRLQANVRESDIVGR
MGGDEFAVLLAQADKETALAKAQSLAEAVRAEPVEFGEWSAPLHISFGVREIEPGADPEAALAEADAAMFLRKRRSA
>Mature_237_residues
MKISGARTEPFAAIRKRALARAGAQVAAREVSAPDSAAFLGLTEADLTPKVVEALQTLMGEIEDLRNEVSVLKLRLNEAQ
GLADMDVLTPVLNRRAFLRELKRVAAFAQRYGSPASVVFFDLDGFKSVNDRFGHAAGDEALKAVAKRLQANVRESDIVGR
MGGDEFAVLLAQADKETALAKAQSLAEAVRAEPVEFGEWSAPLHISFGVREIEPGADPEAALAEADAAMFLRKRRSA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=178, Percent_Identity=37.6404494382022, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787262, Length=155, Percent_Identity=35.4838709677419, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786584, Length=156, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081977, Length=237, Percent_Identity=29.535864978903, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI87082007, Length=126, Percent_Identity=38.0952380952381, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787802, Length=157, Percent_Identity=29.9363057324841, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081881, Length=154, Percent_Identity=34.4155844155844, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=108, Percent_Identity=38.8888888888889, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=153, Percent_Identity=31.3725490196078, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=150, Percent_Identity=28.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=94, Percent_Identity=34.0425531914894, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787056, Length=136, Percent_Identity=34.5588235294118, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 25515; Mature: 25515

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISGARTEPFAAIRKRALARAGAQVAAREVSAPDSAAFLGLTEADLTPKVVEALQTLMG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCHHHHHHHHHHHH
EIEDLRNEVSVLKLRLNEAQGLADMDVLTPVLNRRAFLRELKRVAAFAQRYGSPASVVFF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DLDGFKSVNDRFGHAAGDEALKAVAKRLQANVRESDIVGRMGGDEFAVLLAQADKETALA
ECCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCHHHHHH
KAQSLAEAVRAEPVEFGEWSAPLHISFGVREIEPGADPEAALAEADAAMFLRKRRSA
HHHHHHHHHHCCCCCCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKISGARTEPFAAIRKRALARAGAQVAAREVSAPDSAAFLGLTEADLTPKVVEALQTLMG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCHHHHHHHHHHHH
EIEDLRNEVSVLKLRLNEAQGLADMDVLTPVLNRRAFLRELKRVAAFAQRYGSPASVVFF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DLDGFKSVNDRFGHAAGDEALKAVAKRLQANVRESDIVGRMGGDEFAVLLAQADKETALA
ECCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCHHHHHH
KAQSLAEAVRAEPVEFGEWSAPLHISFGVREIEPGADPEAALAEADAAMFLRKRRSA
HHHHHHHHHHCCCCCCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]