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The map label for this gene is ycgF [H]

Identifier: 16127378

GI number: 16127378

Start: 3395369

End: 3396283

Strand: Direct

Name: ycgF [H]

Synonym: CC_3148

Alternate gene names: 16127378

Gene position: 3395369-3396283 (Clockwise)

Preceding gene: 16127377

Following gene: 16127380

Centisome position: 84.53

GC content: 63.28

Gene sequence:

>915_bases
TTGGAGGCGGGCGTTGCGGGCGCGGCAGGCTGTCGCAGGCGAGCTAAGCCTCGCTTCATCTTTCGCGACGATCCCTGGTG
TCTCGTCCAGGTTCGGGGAATGACCATGTCTAAGGCTTGCAGCGGCTGCCGGGATGGCCAGGACTTCGAGACGCCCTTCT
CGATGGCCTTTCAGCCGATCGTCAAACTGTCGTCAGGCGAGGTCTTCGCGCATGAAGCCCTGGCGCGCGGCGCCGAGGGT
CAGGGCGCGGGCACGGTCCTGTCGACGATTTCGGACCAGAACCGCTATGTGTTCGACCAGCAGTGCCGCGTGAAGGCCAT
CGAGCTGGCCACCACGCTGGACCTTCCCCAAGGCGGCGCGAACCTGTCGATCAACTTCATGCCGAACGCGGTCTATGAGC
CCCGCGCTTGCATTCGCCTGACGCTGGCGACCGCCATGCGGACAGGCTTTCCGCTCAACCGCCTGATCTTCGAGTTCACC
GAGAACGAGCAGCTGGATATCCAGCATGTGCTCAACATCCTGCGCACCTACCGCGCCATGGGCTTCAAGACCGCCATCGA
CGATTTCGGCGCGGGCTATGCGGGCCTTAACCTGTTCGCCAAGTATCAGCCGGACATCGTCAAACTCGACATGGACCTGA
TCCGCGACATCGACAGCGATCGCGGCAAGCGGGTGATCGTCTCGAACCTCCTGAGGATGCTCACCGAACTGGAGATCTTC
GCGGTCTGCGAAGGCGTCGAGACGATGGATGAGCTCTCGGCGCTGCGTGATCTGGGCGTCGACTACGTCCAGGGCTACGT
GATCGCAAAGCCGGTGTTCGAGGGCCTGGCGGCGCCGGACGTGGGCGCGATCATCGCGGCCGGTACGACATCGCCACATC
ACAGCGTTGATAGCGCGCCCCGAATTTCCGCATGA

Upstream 100 bases:

>100_bases
TCTAAGACGACCTCAAGACGGCGGTCGGGACGCTCACGCGTCTCGGCCGCAGCCTTTCTACGACGCCGGCGCGGGCTGTC
CTGCGCCGGCGTCGGCGCGT

Downstream 100 bases:

>100_bases
TCGTCTCGTCGTGGACGAACCCCAGCTTCTCGTAGAGGTGGATGGCGGCTTCGCACTTGCGGTTGGTCAGCAGATAGATC
TTGTCCATCCCAAGGCTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MEAGVAGAAGCRRRAKPRFIFRDDPWCLVQVRGMTMSKACSGCRDGQDFETPFSMAFQPIVKLSSGEVFAHEALARGAEG
QGAGTVLSTISDQNRYVFDQQCRVKAIELATTLDLPQGGANLSINFMPNAVYEPRACIRLTLATAMRTGFPLNRLIFEFT
ENEQLDIQHVLNILRTYRAMGFKTAIDDFGAGYAGLNLFAKYQPDIVKLDMDLIRDIDSDRGKRVIVSNLLRMLTELEIF
AVCEGVETMDELSALRDLGVDYVQGYVIAKPVFEGLAAPDVGAIIAAGTTSPHHSVDSAPRISA

Sequences:

>Translated_304_residues
MEAGVAGAAGCRRRAKPRFIFRDDPWCLVQVRGMTMSKACSGCRDGQDFETPFSMAFQPIVKLSSGEVFAHEALARGAEG
QGAGTVLSTISDQNRYVFDQQCRVKAIELATTLDLPQGGANLSINFMPNAVYEPRACIRLTLATAMRTGFPLNRLIFEFT
ENEQLDIQHVLNILRTYRAMGFKTAIDDFGAGYAGLNLFAKYQPDIVKLDMDLIRDIDSDRGKRVIVSNLLRMLTELEIF
AVCEGVETMDELSALRDLGVDYVQGYVIAKPVFEGLAAPDVGAIIAAGTTSPHHSVDSAPRISA
>Mature_304_residues
MEAGVAGAAGCRRRAKPRFIFRDDPWCLVQVRGMTMSKACSGCRDGQDFETPFSMAFQPIVKLSSGEVFAHEALARGAEG
QGAGTVLSTISDQNRYVFDQQCRVKAIELATTLDLPQGGANLSINFMPNAVYEPRACIRLTLATAMRTGFPLNRLIFEFT
ENEQLDIQHVLNILRTYRAMGFKTAIDDFGAGYAGLNLFAKYQPDIVKLDMDLIRDIDSDRGKRVIVSNLLRMLTELEIF
AVCEGVETMDELSALRDLGVDYVQGYVIAKPVFEGLAAPDVGAIIAAGTTSPHHSVDSAPRISA

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=226, Percent_Identity=30.0884955752212, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790496, Length=229, Percent_Identity=28.3842794759825, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI226510982, Length=234, Percent_Identity=26.4957264957265, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082096, Length=227, Percent_Identity=25.5506607929515, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788502, Length=238, Percent_Identity=26.0504201680672, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788849, Length=151, Percent_Identity=31.7880794701987, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081980, Length=225, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081845, Length=236, Percent_Identity=27.9661016949153, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081743, Length=230, Percent_Identity=26.9565217391304, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081921, Length=237, Percent_Identity=23.2067510548523, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787055, Length=229, Percent_Identity=29.2576419213974, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787541, Length=112, Percent_Identity=30.3571428571429, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 33238; Mature: 33238

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAGVAGAAGCRRRAKPRFIFRDDPWCLVQVRGMTMSKACSGCRDGQDFETPFSMAFQPI
CCCCCCCCHHHHHCCCCCEEEECCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHH
VKLSSGEVFAHEALARGAEGQGAGTVLSTISDQNRYVFDQQCRVKAIELATTLDLPQGGA
HHCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCEEECCCHHEEEEHHHHEECCCCCCC
NLSINFMPNAVYEPRACIRLTLATAMRTGFPLNRLIFEFTENEQLDIQHVLNILRTYRAM
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
GFKTAIDDFGAGYAGLNLFAKYQPDIVKLDMDLIRDIDSDRGKRVIVSNLLRMLTELEIF
CHHHHHHHHCCCHHHHHHHCCCCCCEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AVCEGVETMDELSALRDLGVDYVQGYVIAKPVFEGLAAPDVGAIIAAGTTSPHHSVDSAP
HHHHCHHHHHHHHHHHHCCHHHHHCCCEEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
RISA
CCCC
>Mature Secondary Structure
MEAGVAGAAGCRRRAKPRFIFRDDPWCLVQVRGMTMSKACSGCRDGQDFETPFSMAFQPI
CCCCCCCCHHHHHCCCCCEEEECCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHH
VKLSSGEVFAHEALARGAEGQGAGTVLSTISDQNRYVFDQQCRVKAIELATTLDLPQGGA
HHCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCEEECCCHHEEEEHHHHEECCCCCCC
NLSINFMPNAVYEPRACIRLTLATAMRTGFPLNRLIFEFTENEQLDIQHVLNILRTYRAM
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
GFKTAIDDFGAGYAGLNLFAKYQPDIVKLDMDLIRDIDSDRGKRVIVSNLLRMLTELEIF
CHHHHHHHHCCCHHHHHHHCCCCCCEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AVCEGVETMDELSALRDLGVDYVQGYVIAKPVFEGLAAPDVGAIIAAGTTSPHHSVDSAP
HHHHCHHHHHHHHHHHHCCHHHHHCCCEEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
RISA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]