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The map label for this gene is carA

Identifier: 16127066

GI number: 16127066

Start: 3060951

End: 3062117

Strand: Direct

Name: carA

Synonym: CC_2834

Alternate gene names: 16127066

Gene position: 3060951-3062117 (Clockwise)

Preceding gene: 16127062

Following gene: 16127067

Centisome position: 76.2

GC content: 69.24

Gene sequence:

>1167_bases
ATGTCCCAAGACCTTCTCCCCGGCGTCACTGGCGTTCTGGCCCTGGCCGATGGCGCCATCCTGCAAGGCGTGGGCTGCGG
CGCGGTCGGCGACGCGGTCGGCGAGGTGTGCTTCAACACCGCCATGACCGGCTATCAGGAGATCCTGACCGACCCGTCCT
ACATGGCCCAGATCGTGGCCTTCACCTTCCCGCACGTCGGCAATGTCGGGGTGAACGGCGAAGACGTCGAGCAGATCACC
GGCGTGGCCGAGACGGCCGCCCGCGGCGCCCTGTTCCGCGACGTGCCCACCGTCCAGGCCAACTGGCGCGCCGATGGCGA
CTTCGACGCCTGGATGAAGGCGCGCGGCGTGGTGGGTCTGGCGGGTGTCGACACCCGCGCCCTGACCCGCAAGATCCGCG
AAACCGGCATGCCGCACGGCGTCATCGCCCATTCGCCCGACGGCCAGTTCGACCTCGCCGCCCTGGTCGCCAAGGCCAAG
GCCTGGGCGGGCCTCGAAGGCCTCGACCTGGCCAAGGACGCCTCGACGACCCAGACCTTCACCTGGGACGAGGGTCTGTG
GTCGTGGCCGGAAGGCTACGCCAAGCTGGACAAGCCCAAGTACGAGGTCGTGGTCATCGACTACGGCGTCAAGCGCAACA
TCCTGCGCGCCCTGGCCCATGTCGGCGCCCGCGCCACGGTGGTGCCGGCCTCGACCAGCGCCGAAGACATCCTGGCCCGC
AACCCTGACGGTGTGATGCTGAGCAACGGCCCGGGCGACCCGGCCGCCACCGGCCAGTACGCCGTGCCCGAGATCCAGAA
GCTGGTCGCCAGCGGCAAGCCGGTGTTCGGCATTTGCCTGGGCCACCAGATGCTGGCCCTGGCGCTGGGCGCCAAGACCG
TGAAGATGGAACAGGGCCACCACGGCGCCAACCACCCGGTCAAGGACCTGACCACCGGCAAGGTCGAGATCGTCTCGATG
AACCACGGCTTCACCGTCGACACCGCCTCCCTGCCCGCCCCGGTCAAGGAGACCCACGTCTCGCTGTTCGACGGCACCAA
CGCCGGCATCGCCCTGGACGGCAAGCCGGTGTTCTCGGTGCAGCACCACCCCGAAGCGTCTCCGGGGCCGACGGACAGCC
TGTACCTGTTCGAGCGGTTCGCGGGCCTGATGGACGCGGCGAAGTAA

Upstream 100 bases:

>100_bases
CCGACGCGCGGGGGCCGGACGGGGGTTACTCCGGGTCCGTCTTTTTGCGAAAATGACCGCCCAATTCCGGGCAAGGCCGC
GTTCAGGAGCGCTCCATAGA

Downstream 100 bases:

>100_bases
GGCTAAACCCATGAAATCCCTCTCTCTTCGAGAGAGGGAGGGGCCCGCCGCGCAGCGGCGGGAGGGTGAGTGGTTACGCC
GACTGCCGGTGTGGCCGGGC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSQDLLPGVTGVLALADGAILQGVGCGAVGDAVGEVCFNTAMTGYQEILTDPSYMAQIVAFTFPHVGNVGVNGEDVEQIT
GVAETAARGALFRDVPTVQANWRADGDFDAWMKARGVVGLAGVDTRALTRKIRETGMPHGVIAHSPDGQFDLAALVAKAK
AWAGLEGLDLAKDASTTQTFTWDEGLWSWPEGYAKLDKPKYEVVVIDYGVKRNILRALAHVGARATVVPASTSAEDILAR
NPDGVMLSNGPGDPAATGQYAVPEIQKLVASGKPVFGICLGHQMLALALGAKTVKMEQGHHGANHPVKDLTTGKVEIVSM
NHGFTVDTASLPAPVKETHVSLFDGTNAGIALDGKPVFSVQHHPEASPGPTDSLYLFERFAGLMDAAK

Sequences:

>Translated_388_residues
MSQDLLPGVTGVLALADGAILQGVGCGAVGDAVGEVCFNTAMTGYQEILTDPSYMAQIVAFTFPHVGNVGVNGEDVEQIT
GVAETAARGALFRDVPTVQANWRADGDFDAWMKARGVVGLAGVDTRALTRKIRETGMPHGVIAHSPDGQFDLAALVAKAK
AWAGLEGLDLAKDASTTQTFTWDEGLWSWPEGYAKLDKPKYEVVVIDYGVKRNILRALAHVGARATVVPASTSAEDILAR
NPDGVMLSNGPGDPAATGQYAVPEIQKLVASGKPVFGICLGHQMLALALGAKTVKMEQGHHGANHPVKDLTTGKVEIVSM
NHGFTVDTASLPAPVKETHVSLFDGTNAGIALDGKPVFSVQHHPEASPGPTDSLYLFERFAGLMDAAK
>Mature_387_residues
SQDLLPGVTGVLALADGAILQGVGCGAVGDAVGEVCFNTAMTGYQEILTDPSYMAQIVAFTFPHVGNVGVNGEDVEQITG
VAETAARGALFRDVPTVQANWRADGDFDAWMKARGVVGLAGVDTRALTRKIRETGMPHGVIAHSPDGQFDLAALVAKAKA
WAGLEGLDLAKDASTTQTFTWDEGLWSWPEGYAKLDKPKYEVVVIDYGVKRNILRALAHVGARATVVPASTSAEDILARN
PDGVMLSNGPGDPAATGQYAVPEIQKLVASGKPVFGICLGHQMLALALGAKTVKMEQGHHGANHPVKDLTTGKVEIVSMN
HGFTVDTASLPAPVKETHVSLFDGTNAGIALDGKPVFSVQHHPEASPGPTDSLYLFERFAGLMDAAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=375, Percent_Identity=36, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=32.8165374677003, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=32.8165374677003, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=51.0416666666667, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI193204318, Length=387, Percent_Identity=37.2093023255814, Blast_Score=224, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=36.4116094986807, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=386, Percent_Identity=33.160621761658, Blast_Score=203, Evalue=4e-53,
Organism=Drosophila melanogaster, GI45555749, Length=385, Percent_Identity=37.4025974025974, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24642586, Length=385, Percent_Identity=37.4025974025974, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_CAUCR (Q9A4J7)

Other databases:

- EMBL:   AE005673
- PIR:   B87600
- RefSeq:   NP_421630.1
- ProteinModelPortal:   Q9A4J7
- SMR:   Q9A4J7
- GeneID:   943889
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_2834
- NMPDR:   fig|190650.1.peg.2810
- TIGR:   CC_2834
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40702; Mature: 40570

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 279-279 ACT_SITE 363-363 ACT_SITE 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDLLPGVTGVLALADGAILQGVGCGAVGDAVGEVCFNTAMTGYQEILTDPSYMAQIVA
CCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FTFPHVGNVGVNGEDVEQITGVAETAARGALFRDVPTVQANWRADGDFDAWMKARGVVGL
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCCCCHHHHHHHCCCEEE
AGVDTRALTRKIRETGMPHGVIAHSPDGQFDLAALVAKAKAWAGLEGLDLAKDASTTQTF
CCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TWDEGLWSWPEGYAKLDKPKYEVVVIDYGVKRNILRALAHVGARATVVPASTSAEDILAR
EECCCCCCCCCCHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHC
NPDGVMLSNGPGDPAATGQYAVPEIQKLVASGKPVFGICLGHQMLALALGAKTVKMEQGH
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEEECCC
HGANHPVKDLTTGKVEIVSMNHGFTVDTASLPAPVKETHVSLFDGTNAGIALDGKPVFSV
CCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHEEEEEECCCCCEEECCCEEEEE
QHHPEASPGPTDSLYLFERFAGLMDAAK
ECCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQDLLPGVTGVLALADGAILQGVGCGAVGDAVGEVCFNTAMTGYQEILTDPSYMAQIVA
CCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FTFPHVGNVGVNGEDVEQITGVAETAARGALFRDVPTVQANWRADGDFDAWMKARGVVGL
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCCCCHHHHHHHCCCEEE
AGVDTRALTRKIRETGMPHGVIAHSPDGQFDLAALVAKAKAWAGLEGLDLAKDASTTQTF
CCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TWDEGLWSWPEGYAKLDKPKYEVVVIDYGVKRNILRALAHVGARATVVPASTSAEDILAR
EECCCCCCCCCCHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHC
NPDGVMLSNGPGDPAATGQYAVPEIQKLVASGKPVFGICLGHQMLALALGAKTVKMEQGH
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEEECCC
HGANHPVKDLTTGKVEIVSMNHGFTVDTASLPAPVKETHVSLFDGTNAGIALDGKPVFSV
CCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHEEEEEECCCCCEEECCCEEEEE
QHHPEASPGPTDSLYLFERFAGLMDAAK
ECCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647