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The map label for this gene is gatA [H]

Identifier: 16126850

GI number: 16126850

Start: 2823692

End: 2825086

Strand: Direct

Name: gatA [H]

Synonym: CC_2613

Alternate gene names: 16126850

Gene position: 2823692-2825086 (Clockwise)

Preceding gene: 16126849

Following gene: 16126851

Centisome position: 70.29

GC content: 72.4

Gene sequence:

>1395_bases
GTGAGCTTCTGCAGCGTCGTCGAGATCGCCGGCGAGGTCCGCGCCGGCCGCGTCACCGCACGCGCGGTGACCGAGGCCAC
CCTCTCGCGCATCCAACGCCTGGACGGCGGCATCAACGCCTTCACCGCCGTCACCGCCGAGCGCGCGCTCGCCGCCGCCG
AAGCCGTCGACGCCGATCTCGCGGCGGGACGTCCTGTCGGCCCCTTGGCCGGCGTGCCCTTCGCGGTGAAGAACCTGTTC
GACCTCGAGGGCCTGCCCACCCTGGCCGGATCGAAGATCCGCCGCGCCGCCGCGCCGCCCGCGCATGACGCGACCCTGGT
CCAGCGTCTGACCGCCGCCGGCGCCGTTCTGGTCGGCGCGCTGAACATGGACGAGTTCGCCTACGGCTTCGTCACCGAGA
ACGCCCACGATGGGCCCGTGCGCAATCCGCACGATCCGACGCGGATCGCCGGCGGCTCGTCGGGCGGCTCGGCGGCGGCC
GTCGCGGCGGGCCTCGTGCCCTTGACCCTGGGCTCGGACACTAACGGCTCGATCCGCATTCCCGCCGGCCTGTGCGGCGT
GTTCGGCCTCAAGCCCACCTATGGCCGCCTGTCGCGCCAGGGGGTTTTCCCCTTCGTCGAGAGCCTGGACCATGTCGGCC
CCTTCGCGCGCTCGGTCGAGGATCTGGCCCTGGCCTACGATGTCCTGCAAGGGCCAGACCCGTCGGGCGACCCGATTTGC
ATCCGCGACGCCGAACCGCTGGCGGGTCGCCTCGACGCCCTGGCCGAGCAGCCCTTGCGTGTCGGCGTGCTGGGCGGCTG
GTTCCAGCAAGGCGCCTTCCCCGAGGTGCTGGCGGCGCTAGGCCGGGTCGCCGACGCGCTGGAGGCGCGGGATGAGGTCA
CCCTTCAAAACGCCCAGGCCGCCCGCGCCGCCGCCTTCTGCCTGACGGCGTTCGAGGGTGGCGAGCTGCATCACGAGGAT
CTGGCCAGGCGGGCCATGGACTACGATCCGGCCGTCCGCGACCGTCTGCTGGCCGGCGCCCTGCTGCCCGCCGGCGTGGC
TGAGGCCGCCCAGCGCTTCCGCACGATCTTCCGCGACGAGGTTCGCGAGGTCTTCCAGCGCTACGATATCCTGTTGGCTC
CAGCGTCAGTGTGCCCCGCCCCGCCGATCGGCCAGGCGACCATGGAGATGGACGGGGTTCCGGTATCCGTGCGCAAGAAC
CTCGGGGCTTTCACCCAGCCCATCAGCTATGTGGGCCTGCCGGTGTTGGCCGCGCCGGTGAACCGTCCGGGCCAGTTGCC
GATCGGCGTGCAGATCATCGCCCCGCCCTGGCGCGAGGACCTGGCCTTCGCCGCCGCACTTCGCCTGCAACGCGCCGGCG
TGGTCGCCGCCCATCCGCCGATGGGGGCGCTATGA

Upstream 100 bases:

>100_bases
CGGGCGTAATCGACAACCTCGTCCTGCTGAGGAGCCAGACCGCCCTGTTTGTCGCCGCGCTCGGCGAGGCGGCCGACGAG
GCGCCGGAGACGTTCCAACC

Downstream 100 bases:

>100_bases
TCATCAACGATCCCGCCGTGCTGGCCGAGGTCACCGCCCTCGTCGACGCCTATGAGGTCGCGCTGATGGCCAACGACGTG
GAAGCGCTGGACGCCGCGTT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSFCSVVEIAGEVRAGRVTARAVTEATLSRIQRLDGGINAFTAVTAERALAAAEAVDADLAAGRPVGPLAGVPFAVKNLF
DLEGLPTLAGSKIRRAAAPPAHDATLVQRLTAAGAVLVGALNMDEFAYGFVTENAHDGPVRNPHDPTRIAGGSSGGSAAA
VAAGLVPLTLGSDTNGSIRIPAGLCGVFGLKPTYGRLSRQGVFPFVESLDHVGPFARSVEDLALAYDVLQGPDPSGDPIC
IRDAEPLAGRLDALAEQPLRVGVLGGWFQQGAFPEVLAALGRVADALEARDEVTLQNAQAARAAAFCLTAFEGGELHHED
LARRAMDYDPAVRDRLLAGALLPAGVAEAAQRFRTIFRDEVREVFQRYDILLAPASVCPAPPIGQATMEMDGVPVSVRKN
LGAFTQPISYVGLPVLAAPVNRPGQLPIGVQIIAPPWREDLAFAAALRLQRAGVVAAHPPMGAL

Sequences:

>Translated_464_residues
MSFCSVVEIAGEVRAGRVTARAVTEATLSRIQRLDGGINAFTAVTAERALAAAEAVDADLAAGRPVGPLAGVPFAVKNLF
DLEGLPTLAGSKIRRAAAPPAHDATLVQRLTAAGAVLVGALNMDEFAYGFVTENAHDGPVRNPHDPTRIAGGSSGGSAAA
VAAGLVPLTLGSDTNGSIRIPAGLCGVFGLKPTYGRLSRQGVFPFVESLDHVGPFARSVEDLALAYDVLQGPDPSGDPIC
IRDAEPLAGRLDALAEQPLRVGVLGGWFQQGAFPEVLAALGRVADALEARDEVTLQNAQAARAAAFCLTAFEGGELHHED
LARRAMDYDPAVRDRLLAGALLPAGVAEAAQRFRTIFRDEVREVFQRYDILLAPASVCPAPPIGQATMEMDGVPVSVRKN
LGAFTQPISYVGLPVLAAPVNRPGQLPIGVQIIAPPWREDLAFAAALRLQRAGVVAAHPPMGAL
>Mature_463_residues
SFCSVVEIAGEVRAGRVTARAVTEATLSRIQRLDGGINAFTAVTAERALAAAEAVDADLAAGRPVGPLAGVPFAVKNLFD
LEGLPTLAGSKIRRAAAPPAHDATLVQRLTAAGAVLVGALNMDEFAYGFVTENAHDGPVRNPHDPTRIAGGSSGGSAAAV
AAGLVPLTLGSDTNGSIRIPAGLCGVFGLKPTYGRLSRQGVFPFVESLDHVGPFARSVEDLALAYDVLQGPDPSGDPICI
RDAEPLAGRLDALAEQPLRVGVLGGWFQQGAFPEVLAALGRVADALEARDEVTLQNAQAARAAAFCLTAFEGGELHHEDL
ARRAMDYDPAVRDRLLAGALLPAGVAEAAQRFRTIFRDEVREVFQRYDILLAPASVCPAPPIGQATMEMDGVPVSVRKNL
GAFTQPISYVGLPVLAAPVNRPGQLPIGVQIIAPPWREDLAFAAALRLQRAGVVAAHPPMGAL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=480, Percent_Identity=31.6666666666667, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI195972892, Length=507, Percent_Identity=26.232741617357, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=250, Percent_Identity=31.6, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=400, Percent_Identity=30.25, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=26.4112903225806, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=215, Percent_Identity=33.4883720930233, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=522, Percent_Identity=24.7126436781609, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=35.4166666666667, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=35.4166666666667, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=462, Percent_Identity=26.4069264069264, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=222, Percent_Identity=30.1801801801802, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=373, Percent_Identity=30.8310991957105, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=465, Percent_Identity=26.6666666666667, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=296, Percent_Identity=26.0135135135135, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=492, Percent_Identity=32.7235772357724, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24644968, Length=495, Percent_Identity=26.8686868686869, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=25.1533742331288, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=25.1533742331288, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=25.1533742331288, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=25.1533742331288, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=26.8686868686869, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=23.9350912778905, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=23.9350912778905, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=23.9350912778905, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356731, Length=243, Percent_Identity=33.7448559670782, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648441, Length=425, Percent_Identity=23.7647058823529, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648439, Length=425, Percent_Identity=23.7647058823529, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48285; Mature: 48154

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFCSVVEIAGEVRAGRVTARAVTEATLSRIQRLDGGINAFTAVTAERALAAAEAVDADL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AAGRPVGPLAGVPFAVKNLFDLEGLPTLAGSKIRRAAAPPAHDATLVQRLTAAGAVLVGA
HCCCCCCCCCCCCHHHHHHHHCCCCCCHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHEEE
LNMDEFAYGFVTENAHDGPVRNPHDPTRIAGGSSGGSAAAVAAGLVPLTLGSDTNGSIRI
CCHHHHHEEEEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHCCEEEEECCCCCCCEEC
PAGLCGVFGLKPTYGRLSRQGVFPFVESLDHVGPFARSVEDLALAYDVLQGPDPSGDPIC
CHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEE
IRDAEPLAGRLDALAEQPLRVGVLGGWFQQGAFPEVLAALGRVADALEARDEVTLQNAQA
EECCCCHHHHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAAAFCLTAFEGGELHHEDLARRAMDYDPAVRDRLLAGALLPAGVAEAAQRFRTIFRDE
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VREVFQRYDILLAPASVCPAPPIGQATMEMDGVPVSVRKNLGAFTQPISYVGLPVLAAPV
HHHHHHHHCEEEECHHHCCCCCCCCCCEECCCCCHHHHHHCCHHHCCHHHHCCCCEECCC
NRPGQLPIGVQIIAPPWREDLAFAAALRLQRAGVVAAHPPMGAL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
SFCSVVEIAGEVRAGRVTARAVTEATLSRIQRLDGGINAFTAVTAERALAAAEAVDADL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AAGRPVGPLAGVPFAVKNLFDLEGLPTLAGSKIRRAAAPPAHDATLVQRLTAAGAVLVGA
HCCCCCCCCCCCCHHHHHHHHCCCCCCHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHEEE
LNMDEFAYGFVTENAHDGPVRNPHDPTRIAGGSSGGSAAAVAAGLVPLTLGSDTNGSIRI
CCHHHHHEEEEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHCCEEEEECCCCCCCEEC
PAGLCGVFGLKPTYGRLSRQGVFPFVESLDHVGPFARSVEDLALAYDVLQGPDPSGDPIC
CHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEE
IRDAEPLAGRLDALAEQPLRVGVLGGWFQQGAFPEVLAALGRVADALEARDEVTLQNAQA
EECCCCHHHHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAAAFCLTAFEGGELHHEDLARRAMDYDPAVRDRLLAGALLPAGVAEAAQRFRTIFRDE
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VREVFQRYDILLAPASVCPAPPIGQATMEMDGVPVSVRKNLGAFTQPISYVGLPVLAAPV
HHHHHHHHCEEEECHHHCCCCCCCCCCEECCCCCHHHHHHCCHHHCCHHHHCCCCEECCC
NRPGQLPIGVQIIAPPWREDLAFAAALRLQRAGVVAAHPPMGAL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA