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The map label for this gene is trmFO

Identifier: 16126835

GI number: 16126835

Start: 2807799

End: 2809223

Strand: Direct

Name: trmFO

Synonym: CC_2598

Alternate gene names: 16126835

Gene position: 2807799-2809223 (Clockwise)

Preceding gene: 16126834

Following gene: 16126838

Centisome position: 69.9

GC content: 67.3

Gene sequence:

>1425_bases
ATGACCACGAACTCGACCTATCAGCCTGTCCACGTCATTGGCGGGGGCCTCGCCGGTTCCGAAGCCGCCTGGCAGATCGC
CCAGAGCGGCGTGCCCGTCATCCTGCACGAGATGCGCAAGGACGACGCCACCGGCAAAGTCATCACCGACGCGCACCAGA
CCGACGGCCTGGCCGAGATGGTGTGCTCCAACTCGTTCCGCTCGGACGACTGGCAGTTCAACGCCGTGGGCCTGCTGCAC
GCCGAGATGCGCAAGCTAGGCAGCCTGATCATGTCTTGCGCCGACCAGCACCAGGTGCCGGCCGGCGGCGCCCTGGCCGT
CGACCGCGACGGCTTCTCCACCGAGGTCACTAAGCGCCTGTCGCAGCACCCGCTGGTCACGATCGTCCGCGAGGAGATCG
CGGGCCTGCCGCCTGCCCCTGATGGAAAGAATGGGTGGGACAACGTCATCGTCGCCACCGGCCCCCTCACCTCGCCGGCC
CTGGCCCAGGCGGTCCTGGACCTGACCGGCGAAGGCCAGCTGTCGTTCTTCGACGCCATCGCGCCGATCATCCATTTTGA
GTCCATCAACATGGACATCGCCTGGCGCCAGTCGCGCTACGACAAGGAAGGCCCGGGCGGCGATGCGGCCGCCTATATCA
ACTGCCCGATGAACAAGGAGCAGTACGAGGCTTTCATCGACGCCCTGCTGGCCGGCCCCAAGTCCGAGTTCAAGGAGTGG
GAGAACGTCCCCTATTTCGACGGCTGCCTGCCGATCGAGGTGATGGCCGAGCGCGGCCGCGAGACCCTGCGTCACGGTCC
GATGAAGCCTGTGGGCCTGACCAATCCGCGCGATCCGCTGGTCAAGGCCTACGCCATCGTCCAGCTGCGCCAGGACAACG
CCCTGGGCACGCTGTGGAACATGGTCGGCTTCCAGACCAAGCTGAAGCACGGCGTCCAGGCCGAGACCTTCCGGATGATT
CCGGGCCTGGAAGACGCCCAGTTCGCCCGCCTGGGCGGCCTGCACCGCAACACCTTCATCAACTCGCCCAAGCTCCTGGA
TAAGTCGCTGCGCATGAAGGCCCAGCCGCGCCTGCGTTTCGCCGGCCAGGTCACCGGCGTCGAAGGCTATGTCGAGAGCG
CGGCCATGGGCCTTCTGACCGGCCGGTTCGCCGCCGCCGATCGCAAGGGCGCGCCGATCGACGCCCCGCCGCCGACCACC
GCCCTGGGGGCTCTGGTCGAGCACATCACCGGCGGCCATCTGGAGGCCGGCAACGGTCCTGGCAGCTTCCAGCCGATGAA
CATCAACTATGGCCTGCTGCCGCCGCTCGAAGCGCCGAAGGTCGACGAGGACGGCAAGAAGATCCCGCTGAAGGAACGCG
GCCGCGCCAAGAAGCGGCTGATGAGCCTGCGGGCGCTGAAGGACCTGGACGCCTGGATGGCCTGA

Upstream 100 bases:

>100_bases
ATGTCCCCTCCCCATGCTCGCCAGGCGCGCAGGGCCCGGCCCAGGATCGTGAGCCTACACGAGGTTTCGCCAAACGACAG
CCGGGGGTATAGGGCGCGCC

Downstream 100 bases:

>100_bases
GGCCTCGTCGTCAGGAGGCGGCGGGCCGGCGCAGGCCGCGCGTGGCCTTGTCCTGCGGATAGTAGCGGAAGCCCTTGAGG
ATAATCTTGATGGCTTCCCA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTTNSTYQPVHVIGGGLAGSEAAWQIAQSGVPVILHEMRKDDATGKVITDAHQTDGLAEMVCSNSFRSDDWQFNAVGLLH
AEMRKLGSLIMSCADQHQVPAGGALAVDRDGFSTEVTKRLSQHPLVTIVREEIAGLPPAPDGKNGWDNVIVATGPLTSPA
LAQAVLDLTGEGQLSFFDAIAPIIHFESINMDIAWRQSRYDKEGPGGDAAAYINCPMNKEQYEAFIDALLAGPKSEFKEW
ENVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPLVKAYAIVQLRQDNALGTLWNMVGFQTKLKHGVQAETFRMI
PGLEDAQFARLGGLHRNTFINSPKLLDKSLRMKAQPRLRFAGQVTGVEGYVESAAMGLLTGRFAAADRKGAPIDAPPPTT
ALGALVEHITGGHLEAGNGPGSFQPMNINYGLLPPLEAPKVDEDGKKIPLKERGRAKKRLMSLRALKDLDAWMA

Sequences:

>Translated_474_residues
MTTNSTYQPVHVIGGGLAGSEAAWQIAQSGVPVILHEMRKDDATGKVITDAHQTDGLAEMVCSNSFRSDDWQFNAVGLLH
AEMRKLGSLIMSCADQHQVPAGGALAVDRDGFSTEVTKRLSQHPLVTIVREEIAGLPPAPDGKNGWDNVIVATGPLTSPA
LAQAVLDLTGEGQLSFFDAIAPIIHFESINMDIAWRQSRYDKEGPGGDAAAYINCPMNKEQYEAFIDALLAGPKSEFKEW
ENVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPLVKAYAIVQLRQDNALGTLWNMVGFQTKLKHGVQAETFRMI
PGLEDAQFARLGGLHRNTFINSPKLLDKSLRMKAQPRLRFAGQVTGVEGYVESAAMGLLTGRFAAADRKGAPIDAPPPTT
ALGALVEHITGGHLEAGNGPGSFQPMNINYGLLPPLEAPKVDEDGKKIPLKERGRAKKRLMSLRALKDLDAWMA
>Mature_473_residues
TTNSTYQPVHVIGGGLAGSEAAWQIAQSGVPVILHEMRKDDATGKVITDAHQTDGLAEMVCSNSFRSDDWQFNAVGLLHA
EMRKLGSLIMSCADQHQVPAGGALAVDRDGFSTEVTKRLSQHPLVTIVREEIAGLPPAPDGKNGWDNVIVATGPLTSPAL
AQAVLDLTGEGQLSFFDAIAPIIHFESINMDIAWRQSRYDKEGPGGDAAAYINCPMNKEQYEAFIDALLAGPKSEFKEWE
NVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPLVKAYAIVQLRQDNALGTLWNMVGFQTKLKHGVQAETFRMIP
GLEDAQFARLGGLHRNTFINSPKLLDKSLRMKAQPRLRFAGQVTGVEGYVESAAMGLLTGRFAAADRKGAPIDAPPPTTA
LGALVEHITGGHLEAGNGPGSFQPMNINYGLLPPLEAPKVDEDGKKIPLKERGRAKKRLMSLRALKDLDAWMA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_CAUCR (Q9A566)

Other databases:

- EMBL:   AE005673
- PIR:   C87571
- RefSeq:   NP_421399.1
- ProteinModelPortal:   Q9A566
- GeneID:   943599
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_2598
- NMPDR:   fig|190650.1.peg.2579
- TIGR:   CC_2598
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51368; Mature: 51237

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNSTYQPVHVIGGGLAGSEAAWQIAQSGVPVILHEMRKDDATGKVITDAHQTDGLAEM
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCHHCCHHHHH
VCSNSFRSDDWQFNAVGLLHAEMRKLGSLIMSCADQHQVPAGGALAVDRDGFSTEVTKRL
HHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
SQHPLVTIVREEIAGLPPAPDGKNGWDNVIVATGPLTSPALAQAVLDLTGEGQLSFFDAI
HCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHH
APIIHFESINMDIAWRQSRYDKEGPGGDAAAYINCPMNKEQYEAFIDALLAGPKSEFKEW
HHHHHHHCCCCEEEEHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHH
ENVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPLVKAYAIVQLRQDNALGTLWN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHH
MVGFQTKLKHGVQAETFRMIPGLEDAQFARLGGLHRNTFINSPKLLDKSLRMKAQPRLRF
HHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCCEEE
AGQVTGVEGYVESAAMGLLTGRFAAADRKGAPIDAPPPTTALGALVEHITGGHLEAGNGP
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCC
GSFQPMNINYGLLPPLEAPKVDEDGKKIPLKERGRAKKRLMSLRALKDLDAWMA
CCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTNSTYQPVHVIGGGLAGSEAAWQIAQSGVPVILHEMRKDDATGKVITDAHQTDGLAEM
CCCCCCCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCHHCCHHHHH
VCSNSFRSDDWQFNAVGLLHAEMRKLGSLIMSCADQHQVPAGGALAVDRDGFSTEVTKRL
HHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
SQHPLVTIVREEIAGLPPAPDGKNGWDNVIVATGPLTSPALAQAVLDLTGEGQLSFFDAI
HCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHH
APIIHFESINMDIAWRQSRYDKEGPGGDAAAYINCPMNKEQYEAFIDALLAGPKSEFKEW
HHHHHHHCCCCEEEEHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHH
ENVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPLVKAYAIVQLRQDNALGTLWN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHH
MVGFQTKLKHGVQAETFRMIPGLEDAQFARLGGLHRNTFINSPKLLDKSLRMKAQPRLRF
HHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCCEEE
AGQVTGVEGYVESAAMGLLTGRFAAADRKGAPIDAPPPTTALGALVEHITGGHLEAGNGP
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCC
GSFQPMNINYGLLPPLEAPKVDEDGKKIPLKERGRAKKRLMSLRALKDLDAWMA
CCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647