Click here to switch to the map view.

The map label for this gene is hfsE

Identifier: 16126664

GI number: 16126664

Start: 2630635

End: 2632173

Strand: Direct

Name: hfsE

Synonym: CC_2425

Alternate gene names: 16126664

Gene position: 2630635-2632173 (Clockwise)

Preceding gene: 16126662

Following gene: 16126665

Centisome position: 65.49

GC content: 70.18

Gene sequence:

>1539_bases
GTGGTTTTCCCAACGACGAGCATCCTGCGTCCCGTGTCTCCTCCCCTCTCCCAGGCTGAACCCCTCGCCTTCGCCCCCAC
GCCGGAGACTTCTTCTCCTCGCTCGGGGGACGGAGGAGGCGATCCGTCGGGCGCGGCGAAGCGAGGGCCTTTCCGGCCGG
GCCGACTGGTGCCTTCGCGCGCACGGTTGGAAACACGGCTGCTGGCCCGCACCTTTCGCGCCGTCGACATCCTGATCGCC
GCCGCCGTCGCGGTTGCGGTCACCGCTCTGGGCGCGCCCATTGTGGCGGGTCTTTGGGCCATAGCCGCCTTGATCGGCCT
TCGCCTTGTCGGCGCCTACGCCTTCCCGAGGAAGCAGCCTCTGGCCGCGCACATGACGCGCGCGGGGCTGGCCCTCGGCG
TCAGCCTACTGATGGGGATCGGGGCCAGCCTGGCGACCGGCGGGACCCAAACGCCTGTCGTGTGGACACTTTTGGCGGCG
TCCGGCGCCGTGGCCGGAGCGCATGTCGCCTGGTGGACGGTGATCGCACGCGGCCGCGCCCAAGGGCGACTGACGCCCAA
TATCGTCGTGGTCGGCGCGACCAAGAACGCCGAACGCCTGATCGAGGCGGCCATGCGGACCGGCGAGGTCAATGTGTTGG
GCGTCTTCGACGACCGCCTGGACCGCGCGCCGGCCAACATCCTCGGCGTGCCAGTGTTGGGCGACACCGGCGCCCTGCTC
GACCACCGCATCATGCCCTATGTCGACCGCGTCGTGATCGCGGTGGCCTCGACCGCGCAGACCCGCGTGCGGCAGCTGGT
CGAGAAGCTGGACGTCCTGCCCAACCCGCTGCACCTGTTCATCGATGTCGGCGGCGCGGCCGAGGACGATGCGGCGCTGG
TCCGTATCGCCGCCGGAAGCGAGGCGCCGCTCTCGGGTTCGCCCGCCTCGACCCGTCGCGCCCTGCTCAAGCGCGCCCAG
GATCTGGTGGTCGCGGGCCTGGGCCTCTTGGTCGCCGGACCGGTGATGCTGCTGGTCGCCCTGGCGGTGAAGCTGGACAG
CCCGGGCCCGGTCTTCTTCCGCCAGCGCCGGCACGGCTTCAACAACGAGGCGATTCTCGTCTGGAAGTTCCGCTCGATGC
GGCACGAGATGGCCGACGCCAGCGCCGCCCGCCAGGTCAGCGCCGATGACGACCGCGTCACGCGCGTGGGCAAGTTCATC
CGCAAGACCAGCCTTGATGAGCTGCCCCAGCTGTTCAACGTGCTGACGGGCGAGATGTCGATCGTCGGGCCCCGCCCCCA
CGCCATCGGCATGAAGACCGCCGGCGTGGAGTCGGCCAAGCTGGTCGCCCAGTACGCCCACCGCCATCGCATGAAGCCCG
GCGTCACGGGCTGGGCCGCCATCAAGGGCTCGCGCGGTCCGGTCGACACGCCCGAAAGCGTCCGCCTGCGGGTGGCGCTG
GACGTCGAGTACATCGAGCGTCAGTCGTTCTGGCTGGACCTCTACATCATCGCCATGACCATCCCCTGCCTGTTGGGGGA
TCGGTCCGCCGTGCGCTAG

Upstream 100 bases:

>100_bases
AGGGAGCGGTTCGATAGCGTGTTCGCTTCGCGATGACAACGGCGGATGTCGGAATTTTCCGGTGTTAACGTGACGCTCAG
ACGGGATCGCTAGGCTGAAC

Downstream 100 bases:

>100_bases
GCCGCGCATGTTCTGGCGCGGCGTCCTCGGCTATCTGCCGGTCAACATCGTCCAAGGCGTGGTGGGCCTGCTCACCATCG
TCCTCTTCACGCGCGTGCTG

Product: HfsE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MVFPTTSILRPVSPPLSQAEPLAFAPTPETSSPRSGDGGGDPSGAAKRGPFRPGRLVPSRARLETRLLARTFRAVDILIA
AAVAVAVTALGAPIVAGLWAIAALIGLRLVGAYAFPRKQPLAAHMTRAGLALGVSLLMGIGASLATGGTQTPVVWTLLAA
SGAVAGAHVAWWTVIARGRAQGRLTPNIVVVGATKNAERLIEAAMRTGEVNVLGVFDDRLDRAPANILGVPVLGDTGALL
DHRIMPYVDRVVIAVASTAQTRVRQLVEKLDVLPNPLHLFIDVGGAAEDDAALVRIAAGSEAPLSGSPASTRRALLKRAQ
DLVVAGLGLLVAGPVMLLVALAVKLDSPGPVFFRQRRHGFNNEAILVWKFRSMRHEMADASAARQVSADDDRVTRVGKFI
RKTSLDELPQLFNVLTGEMSIVGPRPHAIGMKTAGVESAKLVAQYAHRHRMKPGVTGWAAIKGSRGPVDTPESVRLRVAL
DVEYIERQSFWLDLYIIAMTIPCLLGDRSAVR

Sequences:

>Translated_512_residues
MVFPTTSILRPVSPPLSQAEPLAFAPTPETSSPRSGDGGGDPSGAAKRGPFRPGRLVPSRARLETRLLARTFRAVDILIA
AAVAVAVTALGAPIVAGLWAIAALIGLRLVGAYAFPRKQPLAAHMTRAGLALGVSLLMGIGASLATGGTQTPVVWTLLAA
SGAVAGAHVAWWTVIARGRAQGRLTPNIVVVGATKNAERLIEAAMRTGEVNVLGVFDDRLDRAPANILGVPVLGDTGALL
DHRIMPYVDRVVIAVASTAQTRVRQLVEKLDVLPNPLHLFIDVGGAAEDDAALVRIAAGSEAPLSGSPASTRRALLKRAQ
DLVVAGLGLLVAGPVMLLVALAVKLDSPGPVFFRQRRHGFNNEAILVWKFRSMRHEMADASAARQVSADDDRVTRVGKFI
RKTSLDELPQLFNVLTGEMSIVGPRPHAIGMKTAGVESAKLVAQYAHRHRMKPGVTGWAAIKGSRGPVDTPESVRLRVAL
DVEYIERQSFWLDLYIIAMTIPCLLGDRSAVR
>Mature_512_residues
MVFPTTSILRPVSPPLSQAEPLAFAPTPETSSPRSGDGGGDPSGAAKRGPFRPGRLVPSRARLETRLLARTFRAVDILIA
AAVAVAVTALGAPIVAGLWAIAALIGLRLVGAYAFPRKQPLAAHMTRAGLALGVSLLMGIGASLATGGTQTPVVWTLLAA
SGAVAGAHVAWWTVIARGRAQGRLTPNIVVVGATKNAERLIEAAMRTGEVNVLGVFDDRLDRAPANILGVPVLGDTGALL
DHRIMPYVDRVVIAVASTAQTRVRQLVEKLDVLPNPLHLFIDVGGAAEDDAALVRIAAGSEAPLSGSPASTRRALLKRAQ
DLVVAGLGLLVAGPVMLLVALAVKLDSPGPVFFRQRRHGFNNEAILVWKFRSMRHEMADASAARQVSADDDRVTRVGKFI
RKTSLDELPQLFNVLTGEMSIVGPRPHAIGMKTAGVESAKLVAQYAHRHRMKPGVTGWAAIKGSRGPVDTPESVRLRVAL
DVEYIERQSFWLDLYIIAMTIPCLLGDRSAVR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=254, Percent_Identity=45.2755905511811, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54240; Mature: 54240

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFPTTSILRPVSPPLSQAEPLAFAPTPETSSPRSGDGGGDPSGAAKRGPFRPGRLVPSR
CCCCCCHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ARLETRLLARTFRAVDILIAAAVAVAVTALGAPIVAGLWAIAALIGLRLVGAYAFPRKQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAAHMTRAGLALGVSLLMGIGASLATGGTQTPVVWTLLAASGAVAGAHVAWWTVIARGRA
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCC
QGRLTPNIVVVGATKNAERLIEAAMRTGEVNVLGVFDDRLDRAPANILGVPVLGDTGALL
CCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCEEEEEEECCCCHHH
DHRIMPYVDRVVIAVASTAQTRVRQLVEKLDVLPNPLHLFIDVGGAAEDDAALVRIAAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCC
EAPLSGSPASTRRALLKRAQDLVVAGLGLLVAGPVMLLVALAVKLDSPGPVFFRQRRHGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
NNEAILVWKFRSMRHEMADASAARQVSADDDRVTRVGKFIRKTSLDELPQLFNVLTGEMS
CCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEE
IVGPRPHAIGMKTAGVESAKLVAQYAHRHRMKPGVTGWAAIKGSRGPVDTPESVRLRVAL
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEE
DVEYIERQSFWLDLYIIAMTIPCLLGDRSAVR
EHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MVFPTTSILRPVSPPLSQAEPLAFAPTPETSSPRSGDGGGDPSGAAKRGPFRPGRLVPSR
CCCCCCHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ARLETRLLARTFRAVDILIAAAVAVAVTALGAPIVAGLWAIAALIGLRLVGAYAFPRKQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAAHMTRAGLALGVSLLMGIGASLATGGTQTPVVWTLLAASGAVAGAHVAWWTVIARGRA
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCC
QGRLTPNIVVVGATKNAERLIEAAMRTGEVNVLGVFDDRLDRAPANILGVPVLGDTGALL
CCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCEEEEEEECCCCHHH
DHRIMPYVDRVVIAVASTAQTRVRQLVEKLDVLPNPLHLFIDVGGAAEDDAALVRIAAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCC
EAPLSGSPASTRRALLKRAQDLVVAGLGLLVAGPVMLLVALAVKLDSPGPVFFRQRRHGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
NNEAILVWKFRSMRHEMADASAARQVSADDDRVTRVGKFIRKTSLDELPQLFNVLTGEMS
CCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEE
IVGPRPHAIGMKTAGVESAKLVAQYAHRHRMKPGVTGWAAIKGSRGPVDTPESVRLRVAL
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEE
DVEYIERQSFWLDLYIIAMTIPCLLGDRSAVR
EHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]