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The map label for this gene is pcaF [H]

Identifier: 16126646

GI number: 16126646

Start: 2611784

End: 2612983

Strand: Direct

Name: pcaF [H]

Synonym: CC_2407

Alternate gene names: 16126646

Gene position: 2611784-2612983 (Clockwise)

Preceding gene: 16126645

Following gene: 16126647

Centisome position: 65.02

GC content: 71.25

Gene sequence:

>1200_bases
ATGACCGCCGCCTATATCTGTGACGGGATCCGCACCCCGATCGGTCGCTATGGTGGGTCGCTCTCAAAGGTGCGCGCCGA
CGATCTGGCGGCGATCCCGCTGAAGGCCTTGGTCGCGCGGAACCCGAGTCTGGACCTGGCTGCGATCGACGAGATCGTCC
TGGGCAGCGCCAACCAGGCGGGCGAGGACAACCGCAACGTCGCCCGCATGGCCCTGCTGCTGGCCGGCTATCCCGTGTCG
GTTCCGGGCGTGACCGTGAACCGCCTCTGCGCCTCGGGTCTGGAGGCCGTGGGCTACGCCGCCCGCGCCATCGCCTCGGG
CCACAACGACCTCGTCATCGCCGGCGGCGTCGAGAGCATGAGCCGCGCGCCGTTCGTGATGGGCAAGGCCGACAGCGCCT
TCTCCCGCAGCGCCGAGATCTTCGACACCACCATCGGCTGGCGCTTCGTGAACCCGGCCATGCGCAAGCTGTACGGCGTC
GACTCCATGCCCGAGACGGCCGAGAACGTCGCCACGGACTATGGCGTCAACCGCGAAGACCAGGACGCTTTCGCCCTGCG
TAGCCAAGCCCGCACCGCCGCCGCCCAGGCCAACGGCTTCCTGGCCGGTGAGATCACGCCTGTCGAGATCCCCGGCAAGG
CGGGTCCGACGATCGTCGACCGCGACGAGCACCCGCGCGAAACCACCATGGAGGCCCTGGCCAAGCTGAAGCCGATCGTC
CGCGAGGGCGGCACGGTCACGGCCGGCAACGCCTCGGGCGTCAATGACGGCGCGGTGGCCCTGGTTATCGCCTCCGAGGA
CGCGGTGAAGCGCCACGGCCTGACGCCACGCGCCCGGATCACCGGCTACGCCAGCGCCGGGGTCGAGCCGCGCGTGATGG
GGATCGGGCCGGTTCCCGCCGTCCGCAAGCTGATGGCCAAGACGGGTCTCGCCATCGGCGACTTCGACGTCGTCGAGTTG
AACGAGGCCTTCGCCGCCCAGGGGCTGGCCGTTCTGCGCCAGCTGGGCCTGCCCGACGATGGCGCGCATGTGAACGCCAA
CGGCGGCGCCATCGCGCTGGGCCATCCGCTCGGCGCGTCCGGCGCGCGCCTGGTCCTCACCGCCCTGCGCCAGTTGGAAG
CCAGCGGCGGCCAGCGCGGCCTGGCCACCCTCTGCATCGGCGTCGGCCAGGGCGCGGCCCTGGCCTTCGAACGCGTCTAG

Upstream 100 bases:

>100_bases
GCAAGGGGGGCGGCCTCACTTTGATCGAGCTGGCTCCTGAGGTCACCCTCGACGAAGTTAAGGCCAAGACGGCAGCCGAT
TTCGATGCGGGAGCCTTCGC

Downstream 100 bases:

>100_bases
GAGCCGCCGATGAACCCGAACTTCAAGCGCGAGGACGCAGGCCAACCGCCGTCGCTGCACGCTCCCTACGCCTCGACCGT
CCCGCGCTCGCCGCGCCAGC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTAAYICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNPSLDLAAIDEIVLGSANQAGEDNRNVARMALLLAGYPVS
VPGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESMSRAPFVMGKADSAFSRSAEIFDTTIGWRFVNPAMRKLYGV
DSMPETAENVATDYGVNREDQDAFALRSQARTAAAQANGFLAGEITPVEIPGKAGPTIVDRDEHPRETTMEALAKLKPIV
REGGTVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVEPRVMGIGPVPAVRKLMAKTGLAIGDFDVVEL
NEAFAAQGLAVLRQLGLPDDGAHVNANGGAIALGHPLGASGARLVLTALRQLEASGGQRGLATLCIGVGQGAALAFERV

Sequences:

>Translated_399_residues
MTAAYICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNPSLDLAAIDEIVLGSANQAGEDNRNVARMALLLAGYPVS
VPGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESMSRAPFVMGKADSAFSRSAEIFDTTIGWRFVNPAMRKLYGV
DSMPETAENVATDYGVNREDQDAFALRSQARTAAAQANGFLAGEITPVEIPGKAGPTIVDRDEHPRETTMEALAKLKPIV
REGGTVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVEPRVMGIGPVPAVRKLMAKTGLAIGDFDVVEL
NEAFAAQGLAVLRQLGLPDDGAHVNANGGAIALGHPLGASGARLVLTALRQLEASGGQRGLATLCIGVGQGAALAFERV
>Mature_398_residues
TAAYICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNPSLDLAAIDEIVLGSANQAGEDNRNVARMALLLAGYPVSV
PGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESMSRAPFVMGKADSAFSRSAEIFDTTIGWRFVNPAMRKLYGVD
SMPETAENVATDYGVNREDQDAFALRSQARTAAAQANGFLAGEITPVEIPGKAGPTIVDRDEHPRETTMEALAKLKPIVR
EGGTVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVEPRVMGIGPVPAVRKLMAKTGLAIGDFDVVELN
EAFAAQGLAVLRQLGLPDDGAHVNANGGAIALGHPLGASGARLVLTALRQLEASGGQRGLATLCIGVGQGAALAFERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=42.0103092783505, Blast_Score=294, Evalue=8e-80,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=41.5816326530612, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI4557237, Length=399, Percent_Identity=37.844611528822, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4501853, Length=404, Percent_Identity=39.3564356435644, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=33.7264150943396, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI194353979, Length=395, Percent_Identity=30.379746835443, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=67.8304239401496, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=46.5174129353234, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=44.7174447174447, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI87082165, Length=399, Percent_Identity=41.3533834586466, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788683, Length=408, Percent_Identity=31.3725490196078, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=397, Percent_Identity=42.8211586901763, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=396, Percent_Identity=35.8585858585859, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=35.264483627204, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=31.4420803782506, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=399, Percent_Identity=30.8270676691729, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17537653, Length=337, Percent_Identity=24.3323442136499, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=38.8613861386139, Blast_Score=233, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=36.7758186397985, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=46, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=37.8109452736318, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=35.5498721227622, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=33.6492890995261, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI19921506, Length=332, Percent_Identity=28.0120481927711, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585051, Length=335, Percent_Identity=28.6567164179104, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41037; Mature: 40906

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAYICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNPSLDLAAIDEIVLGSANQA
CCHHHHHCCHHCCHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
GEDNRNVARMALLLAGYPVSVPGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVMGKADSAFSRSAEIFDTTIGWRFVNPAMRKLYGVDSMPETAENVATDYGVNRED
CCCCEEEECCCHHHHCCHHHHHHHCCCEECCHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
QDAFALRSQARTAAAQANGFLAGEITPVEIPGKAGPTIVDRDEHPRETTMEALAKLKPIV
HHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHH
REGGTVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVEPRVMGIGPVPA
HCCCEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCCEEEEEECCCCCCEEEECCCHHH
VRKLMAKTGLAIGDFDVVELNEAFAAQGLAVLRQLGLPDDGAHVNANGGAIALGHPLGAS
HHHHHHHCCCEECCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCCCCC
GARLVLTALRQLEASGGQRGLATLCIGVGQGAALAFERV
CHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECC
>Mature Secondary Structure 
TAAYICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNPSLDLAAIDEIVLGSANQA
CHHHHHCCHHCCHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
GEDNRNVARMALLLAGYPVSVPGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVMGKADSAFSRSAEIFDTTIGWRFVNPAMRKLYGVDSMPETAENVATDYGVNRED
CCCCEEEECCCHHHHCCHHHHHHHCCCEECCHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
QDAFALRSQARTAAAQANGFLAGEITPVEIPGKAGPTIVDRDEHPRETTMEALAKLKPIV
HHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHH
REGGTVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVEPRVMGIGPVPA
HCCCEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCCEEEEEECCCCCCEEEECCCHHH
VRKLMAKTGLAIGDFDVVELNEAFAAQGLAVLRQLGLPDDGAHVNANGGAIALGHPLGAS
HHHHHHHCCCEECCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCCCCC
GARLVLTALRQLEASGGQRGLATLCIGVGQGAALAFERV
CHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]