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The map label for this gene is yogA [H]

Identifier: 16126592

GI number: 16126592

Start: 2552828

End: 2553802

Strand: Direct

Name: yogA [H]

Synonym: CC_2353

Alternate gene names: 16126592

Gene position: 2552828-2553802 (Clockwise)

Preceding gene: 16126590

Following gene: 16126599

Centisome position: 63.55

GC content: 68.0

Gene sequence:

>975_bases
ATGAAGGCCGTTACCTGCAGACGCTACGGATCGCCCGATATGCTCGCGATCGAGACGATTGAACGCCCAAAGCCGAGGCG
GGACGAGGTTCTGGTGCGCGTCACGAACAGCACCGTGACATCCGGAGACACCCGACTGCGCGGGTTCCGCGGTCCGCTGC
TGTTCTGGCTCCCCTTGCGTTTGGCGTTCGGCGTCTTCCGACCTCGCCAACCGATCACCGGCATGGAGTTCGCCGGAGAC
GTCGTTGCCCTAGGCTCGGCTGTGAAGGGGCTGCGCCCGGGCGATCGCGTCTTCGGCATGAAGCTGGGAGGCGCCAACGC
CGAGTATGTCACGGTGAAGGCGACGGCCGCCTTGGCGCGCTGTCCTGACCGGCTCAGCGACGACCAATGCGCAGCCACCC
CGTTCGGCGCGCTGTCGGCCCTCACCTTCGTCCGGGATACGGCGGAGCTGCGACCGGGCGAACGGATCCTGATCTACGGC
GCCAGCGGCGCGGTCGGGGTCTTCGCCATCCAGCTCGCGAAGATCCTTGGAGCCCACGTCACGGCCGTCAGCAGCGCGCG
CAACCTTCAGATGGTGCTCGACCTGGGGGCCGACCGCGCCCTGGACTACGCCGCCCCGGACTTCCGCCTGGAGGCCGAGG
CCTATGACGTCATCCTGGACACCGTGGGCGCGACGAGGTTCAGCCAGTGCCGCCACGCCCTCGGGCCCACCGGACGACAT
GTCTTCGCCGAGGTCGACGGCGGTCGCCTGCTGCAGTCGCTCTGGACGAGCTTCTGCTCGGGCCCGCGCGTCCTTTGCGG
CTTCTCCGCAGGCGACTCCCCGGAGGACATGACCCTGATCGCCCGACATCTGGATCGCGGCGTGCTGCGGCCGGTTATTG
ATCGCCGCTACACCATGGCCCAGATCATCGAGGCCCATCGCTATGTCGAGCTTGGCCGAAAGCGCGGCGCCGTCGTGATC
CGTGTCGCCGACTAG

Upstream 100 bases:

>100_bases
CGCCAATCCATACGTTTTTGAATATCTGAAATACGATTTTATCGAATTCATATGCGATTTTGCTTCGCGTAGCTACGGGC
TCCGAAACCCGGAGAGCGCC

Downstream 100 bases:

>100_bases
AGCATTTTCCGATCTGTTTGGATCGGAGAAATGCTCTAGCCCTTTGTATTAACGCATTTTCTGACGACGAACCGGATCCA
CTTCGTCGGAAAATGCTCTA

Product: zinc-containing alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKAVTCRRYGSPDMLAIETIERPKPRRDEVLVRVTNSTVTSGDTRLRGFRGPLLFWLPLRLAFGVFRPRQPITGMEFAGD
VVALGSAVKGLRPGDRVFGMKLGGANAEYVTVKATAALARCPDRLSDDQCAATPFGALSALTFVRDTAELRPGERILIYG
ASGAVGVFAIQLAKILGAHVTAVSSARNLQMVLDLGADRALDYAAPDFRLEAEAYDVILDTVGATRFSQCRHALGPTGRH
VFAEVDGGRLLQSLWTSFCSGPRVLCGFSAGDSPEDMTLIARHLDRGVLRPVIDRRYTMAQIIEAHRYVELGRKRGAVVI
RVAD

Sequences:

>Translated_324_residues
MKAVTCRRYGSPDMLAIETIERPKPRRDEVLVRVTNSTVTSGDTRLRGFRGPLLFWLPLRLAFGVFRPRQPITGMEFAGD
VVALGSAVKGLRPGDRVFGMKLGGANAEYVTVKATAALARCPDRLSDDQCAATPFGALSALTFVRDTAELRPGERILIYG
ASGAVGVFAIQLAKILGAHVTAVSSARNLQMVLDLGADRALDYAAPDFRLEAEAYDVILDTVGATRFSQCRHALGPTGRH
VFAEVDGGRLLQSLWTSFCSGPRVLCGFSAGDSPEDMTLIARHLDRGVLRPVIDRRYTMAQIIEAHRYVELGRKRGAVVI
RVAD
>Mature_324_residues
MKAVTCRRYGSPDMLAIETIERPKPRRDEVLVRVTNSTVTSGDTRLRGFRGPLLFWLPLRLAFGVFRPRQPITGMEFAGD
VVALGSAVKGLRPGDRVFGMKLGGANAEYVTVKATAALARCPDRLSDDQCAATPFGALSALTFVRDTAELRPGERILIYG
ASGAVGVFAIQLAKILGAHVTAVSSARNLQMVLDLGADRALDYAAPDFRLEAEAYDVILDTVGATRFSQCRHALGPTGRH
VFAEVDGGRLLQSLWTSFCSGPRVLCGFSAGDSPEDMTLIARHLDRGVLRPVIDRRYTMAQIIEAHRYVELGRKRGAVVI
RVAD

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=346, Percent_Identity=26.878612716763, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI22538444, Length=346, Percent_Identity=26.878612716763, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI47519420, Length=360, Percent_Identity=29.1666666666667, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI18379349, Length=283, Percent_Identity=25.4416961130742, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI194239674, Length=325, Percent_Identity=24.9230769230769, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI13236495, Length=325, Percent_Identity=24.9230769230769, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI194239676, Length=318, Percent_Identity=24.8427672955975, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI24308257, Length=363, Percent_Identity=24.5179063360882, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790485, Length=229, Percent_Identity=29.2576419213974, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507255, Length=331, Percent_Identity=27.4924471299094, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=296, Percent_Identity=23.3108108108108, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24581341, Length=361, Percent_Identity=27.4238227146814, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581339, Length=361, Percent_Identity=27.4238227146814, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35186; Mature: 35186

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVTCRRYGSPDMLAIETIERPKPRRDEVLVRVTNSTVTSGDTRLRGFRGPLLFWLPLR
CCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEECCCCHHCCCCCCHHHHHHHH
LAFGVFRPRQPITGMEFAGDVVALGSAVKGLRPGDRVFGMKLGGANAEYVTVKATAALAR
HHHHHCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEHHHHHH
CPDRLSDDQCAATPFGALSALTFVRDTAELRPGERILIYGASGAVGVFAIQLAKILGAHV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
TAVSSARNLQMVLDLGADRALDYAAPDFRLEAEAYDVILDTVGATRFSQCRHALGPTGRH
HHHHCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCE
VFAEVDGGRLLQSLWTSFCSGPRVLCGFSAGDSPEDMTLIARHLDRGVLRPVIDRRYTMA
EEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIEAHRYVELGRKRGAVVIRVAD
HHHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MKAVTCRRYGSPDMLAIETIERPKPRRDEVLVRVTNSTVTSGDTRLRGFRGPLLFWLPLR
CCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEECCCCHHCCCCCCHHHHHHHH
LAFGVFRPRQPITGMEFAGDVVALGSAVKGLRPGDRVFGMKLGGANAEYVTVKATAALAR
HHHHHCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEHHHHHH
CPDRLSDDQCAATPFGALSALTFVRDTAELRPGERILIYGASGAVGVFAIQLAKILGAHV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
TAVSSARNLQMVLDLGADRALDYAAPDFRLEAEAYDVILDTVGATRFSQCRHALGPTGRH
HHHHCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCE
VFAEVDGGRLLQSLWTSFCSGPRVLCGFSAGDSPEDMTLIARHLDRGVLRPVIDRRYTMA
EEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIEAHRYVELGRKRGAVVIRVAD
HHHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]