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The map label for this gene is purR [H]

Identifier: 16126523

GI number: 16126523

Start: 2483331

End: 2484419

Strand: Direct

Name: purR [H]

Synonym: CC_2284

Alternate gene names: 16126523

Gene position: 2483331-2484419 (Clockwise)

Preceding gene: 16126519

Following gene: 16126526

Centisome position: 61.82

GC content: 67.03

Gene sequence:

>1089_bases
TTGCAAAGCCGGACAAACCGCGCCTTATGGGGCTCTGAAAAAATTTGCAGAAATCTTTGCATTAGGGGCGAGTTCGGGGT
GAGCAAGGGCGCGACGCGACTGGAAGACCTGGCCAAGTTGGCGGGCGTTTCGATCTCGACCGCCTCGCGCGCCCTCAACG
ACAGTCCGGCGGTCAACAAGCGCACCAAGCAGCTGATCTGGAAGCTGGCGCGCGAGATGGACTACCCGTTCCGTCGCTAC
ATGCCCGCCGGCCCGATCGGCGCGGAGGCGACCATCGCCATCGTCACCCCGCGCCCCCAGGGCCGGGGCAGCAGCATGTC
GGACCCGTTCTTCCTGGAACTGTTGGCCGGCGTCGGCGAGGCGGCCCGCGAGCGTGGCTGCGACGTGATCCTGTCGCACA
TCGCGCCGACCAACTATGACGACCTGTCCGAGGCCATGACCACCAGCCGGGCCGAGGGGGTGATCTTCCTGGGCCAAAGC
TCGCTGCACGCCGCGTTCAATCGTCTGGCCGAGACCGAGAACCGCTTCGTGGTCTGGGGCGCGCAATTGCCGGACCAGAA
CTACTGCTCGGTGGGCTCGGACAACGTCATGGGCGGCCGTCGCGCCACGCTGCATCTGCAGCGCCTGGGCCGCAAGCGGA
TCGTCTTCCTGGGCGACACCGAGGCGCCCGAAGCCATGCAGCGCCACCGCGGCTATCTCGACGCCCTGGAGCACGCCAAG
CTGGGCGTTGATCCAGACCTGATCGTGCCCGCCCACTTCGAGGTCGAGTCCGCCGAGGCGGCGGTCGACAGCCTGATCCA
TAACGGCATCACCTTCGATGGCGTCGTCGCCGCCTCGGACCTGATCGCCCTGGGCGCGATCCGCGCGCTGAAGCATGCGG
GCCTCTCGGTTCCGGGTGACGTCTCGGTGATGGGCTTCGACAATGTGCCCTTCAGCCGCTACGCCAGCCCCGCCCTGTCG
ACCATCGCTCAGGACACGACCAAGGCCGGCAAGCTGATGGTCTCCAAGCTGCTCGACAGCGGCGAGCGCGCCAGCCGGTC
CGAGCGCGTGCCGACGGATCTGATCATCCGGGAAAGCTGCGGGGGCTAA

Upstream 100 bases:

>100_bases
TGACTGACCCAGTGATCCGACTTAGCGCCACAAGGGAGAACGGCGACTCTTTGCAAGAGTTTTCAAAGATGAATTTCGAT
CCGAAGCCGCCGCCCTGCTC

Downstream 100 bases:

>100_bases
CCCCGCCGCGCGATCAGCCCCGCGCACACCGGCATCTGCCCCTCACCGCCCAGGTTGACGCCGTCGATCAGCGTCCAGCC
ATCGGGCAGCGACCATGTCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQSRTNRALWGSEKICRNLCIRGEFGVSKGATRLEDLAKLAGVSISTASRALNDSPAVNKRTKQLIWKLAREMDYPFRRY
MPAGPIGAEATIAIVTPRPQGRGSSMSDPFFLELLAGVGEAARERGCDVILSHIAPTNYDDLSEAMTTSRAEGVIFLGQS
SLHAAFNRLAETENRFVVWGAQLPDQNYCSVGSDNVMGGRRATLHLQRLGRKRIVFLGDTEAPEAMQRHRGYLDALEHAK
LGVDPDLIVPAHFEVESAEAAVDSLIHNGITFDGVVAASDLIALGAIRALKHAGLSVPGDVSVMGFDNVPFSRYASPALS
TIAQDTTKAGKLMVSKLLDSGERASRSERVPTDLIIRESCGG

Sequences:

>Translated_362_residues
MQSRTNRALWGSEKICRNLCIRGEFGVSKGATRLEDLAKLAGVSISTASRALNDSPAVNKRTKQLIWKLAREMDYPFRRY
MPAGPIGAEATIAIVTPRPQGRGSSMSDPFFLELLAGVGEAARERGCDVILSHIAPTNYDDLSEAMTTSRAEGVIFLGQS
SLHAAFNRLAETENRFVVWGAQLPDQNYCSVGSDNVMGGRRATLHLQRLGRKRIVFLGDTEAPEAMQRHRGYLDALEHAK
LGVDPDLIVPAHFEVESAEAAVDSLIHNGITFDGVVAASDLIALGAIRALKHAGLSVPGDVSVMGFDNVPFSRYASPALS
TIAQDTTKAGKLMVSKLLDSGERASRSERVPTDLIIRESCGG
>Mature_362_residues
MQSRTNRALWGSEKICRNLCIRGEFGVSKGATRLEDLAKLAGVSISTASRALNDSPAVNKRTKQLIWKLAREMDYPFRRY
MPAGPIGAEATIAIVTPRPQGRGSSMSDPFFLELLAGVGEAARERGCDVILSHIAPTNYDDLSEAMTTSRAEGVIFLGQS
SLHAAFNRLAETENRFVVWGAQLPDQNYCSVGSDNVMGGRRATLHLQRLGRKRIVFLGDTEAPEAMQRHRGYLDALEHAK
LGVDPDLIVPAHFEVESAEAAVDSLIHNGITFDGVVAASDLIALGAIRALKHAGLSVPGDVSVMGFDNVPFSRYASPALS
TIAQDTTKAGKLMVSKLLDSGERASRSERVPTDLIIRESCGG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=344, Percent_Identity=29.9418604651163, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787948, Length=318, Percent_Identity=27.0440251572327, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788474, Length=309, Percent_Identity=27.5080906148867, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=26.6211604095563, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=27.1875, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=24.8407643312102, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789456, Length=295, Percent_Identity=25.0847457627119, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=24.7761194029851, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787906, Length=346, Percent_Identity=24.5664739884393, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39062; Mature: 39062

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSRTNRALWGSEKICRNLCIRGEFGVSKGATRLEDLAKLAGVSISTASRALNDSPAVNK
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHH
RTKQLIWKLAREMDYPFRRYMPAGPIGAEATIAIVTPRPQGRGSSMSDPFFLELLAGVGE
HHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
AARERGCDVILSHIAPTNYDDLSEAMTTSRAEGVIFLGQSSLHAAFNRLAETENRFVVWG
HHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCEEEEE
AQLPDQNYCSVGSDNVMGGRRATLHLQRLGRKRIVFLGDTEAPEAMQRHRGYLDALEHAK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
LGVDPDLIVPAHFEVESAEAAVDSLIHNGITFDGVVAASDLIALGAIRALKHAGLSVPGD
CCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSVMGFDNVPFSRYASPALSTIAQDTTKAGKLMVSKLLDSGERASRSERVPTDLIIRESC
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHEEEECCC
GG
CC
>Mature Secondary Structure
MQSRTNRALWGSEKICRNLCIRGEFGVSKGATRLEDLAKLAGVSISTASRALNDSPAVNK
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHH
RTKQLIWKLAREMDYPFRRYMPAGPIGAEATIAIVTPRPQGRGSSMSDPFFLELLAGVGE
HHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
AARERGCDVILSHIAPTNYDDLSEAMTTSRAEGVIFLGQSSLHAAFNRLAETENRFVVWG
HHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCEEEEE
AQLPDQNYCSVGSDNVMGGRRATLHLQRLGRKRIVFLGDTEAPEAMQRHRGYLDALEHAK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
LGVDPDLIVPAHFEVESAEAAVDSLIHNGITFDGVVAASDLIALGAIRALKHAGLSVPGD
CCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSVMGFDNVPFSRYASPALSTIAQDTTKAGKLMVSKLLDSGERASRSERVPTDLIIRESC
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHEEEECCC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA