Click here to switch to the map view.

The map label for this gene is pepN [H]

Identifier: 16126437

GI number: 16126437

Start: 2404994

End: 2406760

Strand: Direct

Name: pepN [H]

Synonym: CC_2198

Alternate gene names: 16126437

Gene position: 2404994-2406760 (Clockwise)

Preceding gene: 16126436

Following gene: 16126438

Centisome position: 59.87

GC content: 65.93

Gene sequence:

>1767_bases
TTGTCGCGCCGTATCCGCGTTCTCGCCGCCTGCGCGGCTTCCGCCTTGGCGCTCGTCGCCACCGGAGCCTCTGCGCAGAG
CCTCAAGACCAGCCCGCTGAGCCTCGAGACCCGCGGCGCCATGCCCGTCGAGCAACAGGCGCTGGCGTTCGATCACGCCG
ATCTGAAGCTGAAGATCCTGCCCGAGAAGAAGGCCATCGAGGGCGAGGCCACCCTCACCTTCACGGCCCGATCCAGGCTC
GACAAGCTGGTGGTCGACTTCGACCGAGTCTTCACGATCCGCAGGCTGACGATCGACGGCAAGGCGCTCAAGCCGGGCGC
CTGGAGCAACCCTGAGGGCCGGCTCACTGTCACCCTGCCCCGGAAGGTGGCCAAGGGTCGGTCGGTGACCCTGGCGATCA
CCTATGACGGCGTTCCCCACGAGGCCAAGCGCGCGCCCTGGGACGGCGGCTTTGTCTGGAGCAAGACCGAGACCGGCGAG
CCGTGGATCGCCACGGCGGTGCAGGGCGACGGCTGCGACCTCTTCTGGCCCTGCATCGACTATCCGACCGGCGAGCCCAA
GCTGATTGACCTGCACATCACCGTGCCCGCCCCGCTGGTCGCTCCCGCCAACGGCGCCTTCAAGGGCATGACCGGGCAGG
ACGGCTGGCGCACCTATCACTGGCGGGCGCGCAACCCCAACACCTACGCGATCGCGCTGACCGTCGGCCCCTACGAGGAA
CTGACCGGCGTCTACAAGAGCCGGTTCGGCGACAGCATTCCCATGTCGTACTGGCACCTGAAGGGCCGCGCCGAGAAGGC
CAAGGGCCTGTTCGCCGAGTTCACGCCGATGGTCGACTTCTTCGAGCAGATGATCGGCCCCTACCCCTTCCGGGACGAGA
AGATCGGCGTCGTCGAGACCCCGCACCTGGGCATGGAGCACCAGACGATCAACGCCTACGGCAACGAGTACCGGCTCGAC
GGCTATGGCTTTGACTGGCTGCTGCAGCATGAGTTCGCGCACGAGTGGTTCGGCAACCAACTCACCAACGCCGACAACGA
CGACATGTGGCTGCACGAGGGGTTTGGCACCTACATGCAGCCGCTCTACTCGCAGTGGCTGCACGGCGACATGGAGTATA
TGAGCCGGCTGAACACCCAGCGCCTGGCCGTGCGCAACAAGTTCCCGATCGTCTCGGGCCGTCCGCTCAAGGAGGACGCC
GTCTACAATGGCGATCGCGGACCGGGGAACGACATCTACTACAAGGCCTCGAACGTCCTTCACACGCTGCGCACGCTGAT
CGGCGACGAGGCGTTCTTCAGGATCACGCGGATCGCCGTCTATGGCCGCGATGATCCAAAGCCCGGAAACTTCAAGCCGC
GCCACCTGGGCACGAAAGACTTCGTGAAGATCGTCAATCAGGTGACCGGCAAGGACTATGGCTGGTTCTTCGACGTCTAT
CTCTACGACGCCGCAGCCCCCGAACTGGTCGAGACCCGCGACGGCGTCGATCTGGTGCTGACCTGGAAGACCGCCGGCGA
CAAGCCCTTCCCGATGCCGATGGAGGTGAAGGTCGGCGATCGGACCGTCACGCTCGCCATGACGGGCGGAACGGATCGGG
TGAGCGTGGGCGACGCCACGCCGGTGATCATCGATCCGGCGTCCAAGGTCCTGCGGCGACAGCCCTATGTCGAGGCCTAT
CAGGCCTGGAGAGCCGCCGCCGACAAGGCGGCCGCCGAGGCCCGCAAGAAGGCCGCAGAGGAGAAGGCGGCGGCGGAGAA
GAAGTAG

Upstream 100 bases:

>100_bases
CCGCGCGCGTTTCGACTCCATTTTCCTGGCCGGATGCTTGGCGTCTCGGGCGCGCGCGCCTACCGATACCCCCTGATCGT
TCGGGTTGGGGAGTCGTTCA

Downstream 100 bases:

>100_bases
TCTTCAGCGCAGGCGGACGGGTCTGGTGATCTCTCGCTAACCCTGTCGCTTTACCGAATGGCGCGATCTTTCCCCTAGGG
TCAGCTCGCCCCGAGGAGAT

Product: peptidase M1 family protein

Products: NA

Alternate protein names: Alanine aminopeptidase; Lysyl aminopeptidase; Lys-AP [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSRRIRVLAACAASALALVATGASAQSLKTSPLSLETRGAMPVEQQALAFDHADLKLKILPEKKAIEGEATLTFTARSRL
DKLVVDFDRVFTIRRLTIDGKALKPGAWSNPEGRLTVTLPRKVAKGRSVTLAITYDGVPHEAKRAPWDGGFVWSKTETGE
PWIATAVQGDGCDLFWPCIDYPTGEPKLIDLHITVPAPLVAPANGAFKGMTGQDGWRTYHWRARNPNTYAIALTVGPYEE
LTGVYKSRFGDSIPMSYWHLKGRAEKAKGLFAEFTPMVDFFEQMIGPYPFRDEKIGVVETPHLGMEHQTINAYGNEYRLD
GYGFDWLLQHEFAHEWFGNQLTNADNDDMWLHEGFGTYMQPLYSQWLHGDMEYMSRLNTQRLAVRNKFPIVSGRPLKEDA
VYNGDRGPGNDIYYKASNVLHTLRTLIGDEAFFRITRIAVYGRDDPKPGNFKPRHLGTKDFVKIVNQVTGKDYGWFFDVY
LYDAAAPELVETRDGVDLVLTWKTAGDKPFPMPMEVKVGDRTVTLAMTGGTDRVSVGDATPVIIDPASKVLRRQPYVEAY
QAWRAAADKAAAEARKKAAEEKAAAEKK

Sequences:

>Translated_588_residues
MSRRIRVLAACAASALALVATGASAQSLKTSPLSLETRGAMPVEQQALAFDHADLKLKILPEKKAIEGEATLTFTARSRL
DKLVVDFDRVFTIRRLTIDGKALKPGAWSNPEGRLTVTLPRKVAKGRSVTLAITYDGVPHEAKRAPWDGGFVWSKTETGE
PWIATAVQGDGCDLFWPCIDYPTGEPKLIDLHITVPAPLVAPANGAFKGMTGQDGWRTYHWRARNPNTYAIALTVGPYEE
LTGVYKSRFGDSIPMSYWHLKGRAEKAKGLFAEFTPMVDFFEQMIGPYPFRDEKIGVVETPHLGMEHQTINAYGNEYRLD
GYGFDWLLQHEFAHEWFGNQLTNADNDDMWLHEGFGTYMQPLYSQWLHGDMEYMSRLNTQRLAVRNKFPIVSGRPLKEDA
VYNGDRGPGNDIYYKASNVLHTLRTLIGDEAFFRITRIAVYGRDDPKPGNFKPRHLGTKDFVKIVNQVTGKDYGWFFDVY
LYDAAAPELVETRDGVDLVLTWKTAGDKPFPMPMEVKVGDRTVTLAMTGGTDRVSVGDATPVIIDPASKVLRRQPYVEAY
QAWRAAADKAAAEARKKAAEEKAAAEKK
>Mature_587_residues
SRRIRVLAACAASALALVATGASAQSLKTSPLSLETRGAMPVEQQALAFDHADLKLKILPEKKAIEGEATLTFTARSRLD
KLVVDFDRVFTIRRLTIDGKALKPGAWSNPEGRLTVTLPRKVAKGRSVTLAITYDGVPHEAKRAPWDGGFVWSKTETGEP
WIATAVQGDGCDLFWPCIDYPTGEPKLIDLHITVPAPLVAPANGAFKGMTGQDGWRTYHWRARNPNTYAIALTVGPYEEL
TGVYKSRFGDSIPMSYWHLKGRAEKAKGLFAEFTPMVDFFEQMIGPYPFRDEKIGVVETPHLGMEHQTINAYGNEYRLDG
YGFDWLLQHEFAHEWFGNQLTNADNDDMWLHEGFGTYMQPLYSQWLHGDMEYMSRLNTQRLAVRNKFPIVSGRPLKEDAV
YNGDRGPGNDIYYKASNVLHTLRTLIGDEAFFRITRIAVYGRDDPKPGNFKPRHLGTKDFVKIVNQVTGKDYGWFFDVYL
YDAAAPELVETRDGVDLVLTWKTAGDKPFPMPMEVKVGDRTVTLAMTGGTDRVSVGDATPVIIDPASKVLRRQPYVEAYQ
AWRAAADKAAAEARKKAAEEKAAAEKK

Specific function: Aminopeptidase with broad substrate specificity to several peptides. Shows strong preference for leucine but cleaves also next to Arg and lysine in peptide-bond-containing substrates [H]

COG id: COG0308

COG function: function code E; Aminopeptidase N

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M1 family [H]

Homologues:

Organism=Homo sapiens, GI4505029, Length=323, Percent_Identity=24.7678018575851, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17544504, Length=323, Percent_Identity=23.5294117647059, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71984353, Length=406, Percent_Identity=21.9211822660099, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71984339, Length=408, Percent_Identity=22.0588235294118, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565628, Length=291, Percent_Identity=26.1168384879725, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012778
- InterPro:   IPR001930
- InterPro:   IPR014782 [H]

Pfam domain/function: PF01433 Peptidase_M1 [H]

EC number: =3.4.11.2 [H]

Molecular weight: Translated: 65663; Mature: 65532

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRIRVLAACAASALALVATGASAQSLKTSPLSLETRGAMPVEQQALAFDHADLKLKIL
CCCCCHHHHHHHHHHHEEEECCCCHHHCCCCCCEEECCCCCCCHHHHHEECCCCEEEEEC
PEKKAIEGEATLTFTARSRLDKLVVDFDRVFTIRRLTIDGKALKPGAWSNPEGRLTVTLP
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHEEEEEEEEECCCEECCCCCCCCCCCEEEEEC
RKVAKGRSVTLAITYDGVPHEAKRAPWDGGFVWSKTETGEPWIATAVQGDGCDLFWPCID
HHHHCCCEEEEEEEECCCCCHHHCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECC
YPTGEPKLIDLHITVPAPLVAPANGAFKGMTGQDGWRTYHWRARNPNTYAIALTVGPYEE
CCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCHHH
LTGVYKSRFGDSIPMSYWHLKGRAEKAKGLFAEFTPMVDFFEQMIGPYPFRDEKIGVVET
HHHHHHHHCCCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEC
PHLGMEHQTINAYGNEYRLDGYGFDWLLQHEFAHEWFGNQLTNADNDDMWLHEGFGTYMQ
CCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
PLYSQWLHGDMEYMSRLNTQRLAVRNKFPIVSGRPLKEDAVYNGDRGPGNDIYYKASNVL
HHHHHHHCCCHHHHHHCCHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHH
HTLRTLIGDEAFFRITRIAVYGRDDPKPGNFKPRHLGTKDFVKIVNQVTGKDYGWFFDVY
HHHHHHHCCHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
LYDAAAPELVETRDGVDLVLTWKTAGDKPFPMPMEVKVGDRTVTLAMTGGTDRVSVGDAT
EECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCEEEEECCEEEEEEEECCCCEEECCCCC
PVIIDPASKVLRRQPYVEAYQAWRAAADKAAAEARKKAAEEKAAAEKK
CEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRRIRVLAACAASALALVATGASAQSLKTSPLSLETRGAMPVEQQALAFDHADLKLKIL
CCCCHHHHHHHHHHHEEEECCCCHHHCCCCCCEEECCCCCCCHHHHHEECCCCEEEEEC
PEKKAIEGEATLTFTARSRLDKLVVDFDRVFTIRRLTIDGKALKPGAWSNPEGRLTVTLP
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHEEEEEEEEECCCEECCCCCCCCCCCEEEEEC
RKVAKGRSVTLAITYDGVPHEAKRAPWDGGFVWSKTETGEPWIATAVQGDGCDLFWPCID
HHHHCCCEEEEEEEECCCCCHHHCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECC
YPTGEPKLIDLHITVPAPLVAPANGAFKGMTGQDGWRTYHWRARNPNTYAIALTVGPYEE
CCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCHHH
LTGVYKSRFGDSIPMSYWHLKGRAEKAKGLFAEFTPMVDFFEQMIGPYPFRDEKIGVVET
HHHHHHHHCCCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEC
PHLGMEHQTINAYGNEYRLDGYGFDWLLQHEFAHEWFGNQLTNADNDDMWLHEGFGTYMQ
CCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
PLYSQWLHGDMEYMSRLNTQRLAVRNKFPIVSGRPLKEDAVYNGDRGPGNDIYYKASNVL
HHHHHHHCCCHHHHHHCCHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHH
HTLRTLIGDEAFFRITRIAVYGRDDPKPGNFKPRHLGTKDFVKIVNQVTGKDYGWFFDVY
HHHHHHHCCHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
LYDAAAPELVETRDGVDLVLTWKTAGDKPFPMPMEVKVGDRTVTLAMTGGTDRVSVGDAT
EECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCEEEEECCEEEEEEEECCCCEEECCCCC
PVIIDPASKVLRRQPYVEAYQAWRAAADKAAAEARKKAAEEKAAAEKK
CEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7909302; 7765336 [H]