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The map label for this gene is lipB

Identifier: 16126415

GI number: 16126415

Start: 2383462

End: 2384142

Strand: Direct

Name: lipB

Synonym: CC_2176

Alternate gene names: 16126415

Gene position: 2383462-2384142 (Clockwise)

Preceding gene: 16126410

Following gene: 16126416

Centisome position: 59.34

GC content: 70.93

Gene sequence:

>681_bases
ATGAGGCGCGATGACGCCGCGCCCGTGGGGTGGGCGGTTTCGACCCAGCCCGTCCCCTACCCGGCAGCCGTTGCCGCCAT
GGAGGCCCGCGCGGCGGCCATCGCGGACGGAACGGCCGGCGAGCTGATCTGGCTTTTGGAGCATCCGCCGCTCTACACGG
CCGGGGTCTCGGCCAAGGCCGGCGACCTGATCCAGCCCGACCGCTTTCCGGTGTTCGAGAGCGGGCGCGGCGGCCAGTTC
ACCTATCACGGCCCCGGCCAGCGCGTGGCCTATGTGATGCTGGACCTGACCCAGCGCGGCCGCGACGTCCGCGCCTTCGT
GGCCGCCCTGGAGGCCTGGATCATCGACGCCCTGGCCGCCTTCAACGTCACGGGCGAACTGCGTGAGGGGCGTGTCGGGG
TCTGGGTCGAGCGCAAGGGCGCGGGCTGGAGCCGCGAGGACAAGATCGCCGCCATCGGGGTGAAGCTGCGGCGCTGGGTC
AGCTTTCACGGGATCAGCCTGAACGTGGAGCCGGATCTCTCGCACTTCTCGGGCATCGTGCCCTGCGGCCAGACCGAGCA
CGGCGTCACCAGCCTGGTGGATCTGGGCCTGCCGGTAACCCTGGATGATGCGGACGCGGCGCTGCGGGCGAGCTTTTCGA
AGGTGTTCGGGCCGGTCGAGGACGCTGAGGCGCCGGTTTAG

Upstream 100 bases:

>100_bases
CTGACACGCACGACCTGACTGGTCGCGGCGTCCAGGATTGCAAGAATAAGGCCGCATGCTTTCGCTCCCGTTAAACCCTG
AAAACACTGGCCTTCCGCGC

Downstream 100 bases:

>100_bases
GGGCATGGGGCCTCCGCTTCGCGGCTCCCTTCCCCTTGATGGGGAAGGGCTGGGGATGGGGTGACCTGCTGAACTGGCGG
CTGTGTGCTGCATTGAGCGT

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRRDDAAPVGWAVSTQPVPYPAAVAAMEARAAAIADGTAGELIWLLEHPPLYTAGVSAKAGDLIQPDRFPVFESGRGGQF
TYHGPGQRVAYVMLDLTQRGRDVRAFVAALEAWIIDALAAFNVTGELREGRVGVWVERKGAGWSREDKIAAIGVKLRRWV
SFHGISLNVEPDLSHFSGIVPCGQTEHGVTSLVDLGLPVTLDDADAALRASFSKVFGPVEDAEAPV

Sequences:

>Translated_226_residues
MRRDDAAPVGWAVSTQPVPYPAAVAAMEARAAAIADGTAGELIWLLEHPPLYTAGVSAKAGDLIQPDRFPVFESGRGGQF
TYHGPGQRVAYVMLDLTQRGRDVRAFVAALEAWIIDALAAFNVTGELREGRVGVWVERKGAGWSREDKIAAIGVKLRRWV
SFHGISLNVEPDLSHFSGIVPCGQTEHGVTSLVDLGLPVTLDDADAALRASFSKVFGPVEDAEAPV
>Mature_226_residues
MRRDDAAPVGWAVSTQPVPYPAAVAAMEARAAAIADGTAGELIWLLEHPPLYTAGVSAKAGDLIQPDRFPVFESGRGGQF
TYHGPGQRVAYVMLDLTQRGRDVRAFVAALEAWIIDALAAFNVTGELREGRVGVWVERKGAGWSREDKIAAIGVKLRRWV
SFHGISLNVEPDLSHFSGIVPCGQTEHGVTSLVDLGLPVTLDDADAALRASFSKVFGPVEDAEAPV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=189, Percent_Identity=33.8624338624339, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI87081767, Length=165, Percent_Identity=42.4242424242424, Blast_Score=130, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6323268, Length=180, Percent_Identity=28.8888888888889, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_CAUCR (Q9A6B8)

Other databases:

- EMBL:   AE005673
- PIR:   G87518
- RefSeq:   NP_420979.1
- ProteinModelPortal:   Q9A6B8
- SMR:   Q9A6B8
- GeneID:   943099
- GenomeReviews:   AE005673_GR
- KEGG:   ccr:CC_2176
- NMPDR:   fig|190650.1.peg.2159
- TIGR:   CC_2176
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   PRK14341
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24157; Mature: 24157

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRDDAAPVGWAVSTQPVPYPAAVAAMEARAAAIADGTAGELIWLLEHPPLYTAGVSAKA
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCC
GDLIQPDRFPVFESGRGGQFTYHGPGQRVAYVMLDLTQRGRDVRAFVAALEAWIIDALAA
CCCCCCCCCCCEECCCCCEEEEECCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHH
FNVTGELREGRVGVWVERKGAGWSREDKIAAIGVKLRRWVSFHGISLNVEPDLSHFSGIV
HCCCCCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHHHHEECCEEEEECCCHHHHCCCC
PCGQTEHGVTSLVDLGLPVTLDDADAALRASFSKVFGPVEDAEAPV
CCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRRDDAAPVGWAVSTQPVPYPAAVAAMEARAAAIADGTAGELIWLLEHPPLYTAGVSAKA
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCC
GDLIQPDRFPVFESGRGGQFTYHGPGQRVAYVMLDLTQRGRDVRAFVAALEAWIIDALAA
CCCCCCCCCCCEECCCCCEEEEECCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHH
FNVTGELREGRVGVWVERKGAGWSREDKIAAIGVKLRRWVSFHGISLNVEPDLSHFSGIV
HCCCCCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHHHHEECCEEEEECCCHHHHCCCC
PCGQTEHGVTSLVDLGLPVTLDDADAALRASFSKVFGPVEDAEAPV
CCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647