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The map label for this gene is fadE25 [H]

Identifier: 16126290

GI number: 16126290

Start: 2255477

End: 2256682

Strand: Direct

Name: fadE25 [H]

Synonym: CC_2049

Alternate gene names: 16126290

Gene position: 2255477-2256682 (Clockwise)

Preceding gene: 16126289

Following gene: 16126291

Centisome position: 56.15

GC content: 66.75

Gene sequence:

>1206_bases
ATGGCCGATTTCGGGGCAACCGATCTCGACGCCTTCCGCGCCGACACCCGCGCCTGGCTCGAAGCCAACTATCCAAAATC
CCTGAACGCGCCGATGGGCGAAGACGAGGCCCCGTGGGGCGGCCGCAAGATGGTGTGGAAGAACCCCGACGCCAAGCTGT
GGCTCGACCGCATGGCCGAACGCGGCTGGACCGCCCCGATGTGGCCCAAGGAATACGGCGGCGGCGGCCTCTCCAAGGCC
CAGAACAAGGTGCTGGAACAGGAGCTTCGCCGCCTCAAGGCGCGCCCGGCCCTGATGAGCTTCGGCGTCTGGATGCTGGG
CCCGGTGCTGCTGGAATACGCCACCGAGGAGCAGAAGCAGCGTTTCCTGCCCGCGATCGTCCGCGGCGAAACCCGCTGGT
GCCAGGGCTACAGCGAACCCGGTGCGGGCTCTGACCTCGCCTCGCTGCAGACCAAGTGCGAGGACAAGGGTGACCACTAT
CTGATCAACGGCCAGAAGGTCTGGACCAGCTACGCCGACCAGGCCGACTGGATCTTCTGTCTGGTGCGCACCGACACCAG
CAAGAAGCACGAGGGCATCTCGTTCGTGCTGTTCGACATGACCTCGCCCGGCGTCGAGGCCCGCCCGATCAAGCTGATCA
GCGGCTCCTCGCCCTTCTGCGAGACCTTCTTCGACAATGTGAAGGTCCCGAAGGAACAGCTCGTCGGCAAGCTGAACGGC
GGCTGGGACATCGCCAAGCGCTTGCTGCAGTACGAACGCCAGAACATCAGCGCCTCGGGCTTTGGCGGCGGCGGCGGCCT
GTCGGTTGTCGAAGCGGCCAAGAAGGAGCTCGGCGTCGACAGCGAGGGCCGCATCGCTGATGGCGATTTCCGCGCGCGTC
TGGCGGCTCACTCGATGGACGCCCACGCCTTCATGCTGACCGTGCGCCGCGCCGAGGCGGAGTCCAAGCAGGGCTCGGGC
CCCAGCGCCGCCGTCTCGATCATCAAGTACGCCGCCGCCAAGATGAACCAGGAGCGCACCGAGCTGCTGGTCGAGGCCCT
GGGACTTCAGGGCCTGGGCTGGTCGGGCGAAGACTTCAGCGCCGAGGAACTGGCCGCACCCCGCGCCATGCTGCGCGCCA
AGGGCAACTCGATCGAGGGCGGCACCAGCGAGGTCAACCTCAACGTCGTCGCCAAGCGGGTCCTGGGCCTTCTGGATCAC
CAGTAA

Upstream 100 bases:

>100_bases
CGCGCGCCAAGATGCGAACGATCGTTTGAATCAGCTTGCCGCCGCCGTGGCGAGGGGTAGTGTGTCGGCCAAACACCGGT
CCGCTTAGGGAGATGAGACC

Downstream 100 bases:

>100_bases
CCCTCCGAACCGTAAGCCGCGCGCGCCGTCGGAACGGCGCGCCAAGACCAAGACTTTTCGAGGAGCCTGAACATGGCCGT
CCTGACCGAAGAACAGACGA

Product: acyl-CoA dehydrogenase family protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MADFGATDLDAFRADTRAWLEANYPKSLNAPMGEDEAPWGGRKMVWKNPDAKLWLDRMAERGWTAPMWPKEYGGGGLSKA
QNKVLEQELRRLKARPALMSFGVWMLGPVLLEYATEEQKQRFLPAIVRGETRWCQGYSEPGAGSDLASLQTKCEDKGDHY
LINGQKVWTSYADQADWIFCLVRTDTSKKHEGISFVLFDMTSPGVEARPIKLISGSSPFCETFFDNVKVPKEQLVGKLNG
GWDIAKRLLQYERQNISASGFGGGGGLSVVEAAKKELGVDSEGRIADGDFRARLAAHSMDAHAFMLTVRRAEAESKQGSG
PSAAVSIIKYAAAKMNQERTELLVEALGLQGLGWSGEDFSAEELAAPRAMLRAKGNSIEGGTSEVNLNVVAKRVLGLLDH
Q

Sequences:

>Translated_401_residues
MADFGATDLDAFRADTRAWLEANYPKSLNAPMGEDEAPWGGRKMVWKNPDAKLWLDRMAERGWTAPMWPKEYGGGGLSKA
QNKVLEQELRRLKARPALMSFGVWMLGPVLLEYATEEQKQRFLPAIVRGETRWCQGYSEPGAGSDLASLQTKCEDKGDHY
LINGQKVWTSYADQADWIFCLVRTDTSKKHEGISFVLFDMTSPGVEARPIKLISGSSPFCETFFDNVKVPKEQLVGKLNG
GWDIAKRLLQYERQNISASGFGGGGGLSVVEAAKKELGVDSEGRIADGDFRARLAAHSMDAHAFMLTVRRAEAESKQGSG
PSAAVSIIKYAAAKMNQERTELLVEALGLQGLGWSGEDFSAEELAAPRAMLRAKGNSIEGGTSEVNLNVVAKRVLGLLDH
Q
>Mature_400_residues
ADFGATDLDAFRADTRAWLEANYPKSLNAPMGEDEAPWGGRKMVWKNPDAKLWLDRMAERGWTAPMWPKEYGGGGLSKAQ
NKVLEQELRRLKARPALMSFGVWMLGPVLLEYATEEQKQRFLPAIVRGETRWCQGYSEPGAGSDLASLQTKCEDKGDHYL
INGQKVWTSYADQADWIFCLVRTDTSKKHEGISFVLFDMTSPGVEARPIKLISGSSPFCETFFDNVKVPKEQLVGKLNGG
WDIAKRLLQYERQNISASGFGGGGGLSVVEAAKKELGVDSEGRIADGDFRARLAAHSMDAHAFMLTVRRAEAESKQGSGP
SAAVSIIKYAAAKMNQERTELLVEALGLQGLGWSGEDFSAEELAAPRAMLRAKGNSIEGGTSEVNLNVVAKRVLGLLDHQ

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=329, Percent_Identity=27.9635258358663, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI226958414, Length=367, Percent_Identity=27.2479564032698, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI226958412, Length=354, Percent_Identity=27.4011299435028, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI4557233, Length=359, Percent_Identity=28.4122562674095, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4557231, Length=292, Percent_Identity=27.7397260273973, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI187960098, Length=292, Percent_Identity=27.7397260273973, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI7656849, Length=214, Percent_Identity=28.5046728971963, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21361497, Length=282, Percent_Identity=27.6595744680851, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI4557235, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI76496475, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI38505218, Length=305, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503943, Length=187, Percent_Identity=28.8770053475936, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4501857, Length=296, Percent_Identity=25.6756756756757, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI211971008, Length=256, Percent_Identity=26.5625, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI48976061, Length=256, Percent_Identity=26.5625, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI7669494, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=346, Percent_Identity=21.6763005780347, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081958, Length=353, Percent_Identity=22.6628895184136, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538396, Length=351, Percent_Identity=28.7749287749288, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17508101, Length=348, Percent_Identity=28.448275862069, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17570075, Length=353, Percent_Identity=28.328611898017, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17506239, Length=347, Percent_Identity=28.5302593659942, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17569725, Length=348, Percent_Identity=28.735632183908, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32563615, Length=199, Percent_Identity=32.6633165829146, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17534899, Length=206, Percent_Identity=30.0970873786408, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71990804, Length=206, Percent_Identity=29.6116504854369, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551932, Length=216, Percent_Identity=29.6296296296296, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505929, Length=216, Percent_Identity=28.7037037037037, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985184, Length=225, Percent_Identity=30.6666666666667, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71985192, Length=225, Percent_Identity=30.6666666666667, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533517, Length=217, Percent_Identity=29.4930875576037, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71982178, Length=201, Percent_Identity=27.363184079602, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356377, Length=354, Percent_Identity=29.6610169491525, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24666513, Length=341, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24646207, Length=242, Percent_Identity=32.2314049586777, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355753, Length=357, Percent_Identity=27.1708683473389, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24660351, Length=297, Percent_Identity=28.2828282828283, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281363737, Length=226, Percent_Identity=27.4336283185841, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920834, Length=179, Percent_Identity=31.2849162011173, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 43978; Mature: 43847

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFGATDLDAFRADTRAWLEANYPKSLNAPMGEDEAPWGGRKMVWKNPDAKLWLDRMAE
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
RGWTAPMWPKEYGGGGLSKAQNKVLEQELRRLKARPALMSFGVWMLGPVLLEYATEEQKQ
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RFLPAIVRGETRWCQGYSEPGAGSDLASLQTKCEDKGDHYLINGQKVWTSYADQADWIFC
HHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCEEEE
LVRTDTSKKHEGISFVLFDMTSPGVEARPIKLISGSSPFCETFFDNVKVPKEQLVGKLNG
EEECCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHCCCCCHHHHHHCCCC
GWDIAKRLLQYERQNISASGFGGGGGLSVVEAAKKELGVDSEGRIADGDFRARLAAHSMD
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCC
AHAFMLTVRRAEAESKQGSGPSAAVSIIKYAAAKMNQERTELLVEALGLQGLGWSGEDFS
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
AEELAAPRAMLRAKGNSIEGGTSEVNLNVVAKRVLGLLDHQ
HHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADFGATDLDAFRADTRAWLEANYPKSLNAPMGEDEAPWGGRKMVWKNPDAKLWLDRMAE
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
RGWTAPMWPKEYGGGGLSKAQNKVLEQELRRLKARPALMSFGVWMLGPVLLEYATEEQKQ
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RFLPAIVRGETRWCQGYSEPGAGSDLASLQTKCEDKGDHYLINGQKVWTSYADQADWIFC
HHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCEEEE
LVRTDTSKKHEGISFVLFDMTSPGVEARPIKLISGSSPFCETFFDNVKVPKEQLVGKLNG
EEECCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHCCCCCHHHHHHCCCC
GWDIAKRLLQYERQNISASGFGGGGGLSVVEAAKKELGVDSEGRIADGDFRARLAAHSMD
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCC
AHAFMLTVRRAEAESKQGSGPSAAVSIIKYAAAKMNQERTELLVEALGLQGLGWSGEDFS
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
AEELAAPRAMLRAKGNSIEGGTSEVNLNVVAKRVLGLLDHQ
HHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]