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The map label for this gene is rne [H]

Identifier: 16126120

GI number: 16126120

Start: 2071504

End: 2074200

Strand: Direct

Name: rne [H]

Synonym: CC_1877

Alternate gene names: 16126120

Gene position: 2071504-2074200 (Clockwise)

Preceding gene: 16126116

Following gene: 16126121

Centisome position: 51.57

GC content: 67.07

Gene sequence:

>2697_bases
ATGTCGAAGAAGATGCTGATCGACGCAGCACACGCCGAAGAGACGCGTGTGGTCGTCGTGGACGGTACCCGGGTTGAAGA
GTTCGATTTCGAGAGCCAAACCCGCAAACAGCTTCGTGGAAACATCTATCTCGCCAAGGTGACGCGCGTTGAGCCCAGCC
TCCAGGCTGCGTTCATCGAGTACGGCGGCAACCGTCACGGTTTCCTGGCGTTCAACGAGATCCACCCCGACTACTACCAG
ATCCCGGTCGCCGACCGCGAAGCGCTGATGCGCGACGACTCCGGTGACGACGAGGACGACACCCCGATCTCGCGTCGCGC
CTCCGGCGGCGACGACGAAGACGACGTCAATGGCGGCGACCGCGCGGTCGACGATGATGACGATGACGTCGAAGAAGAAC
TGGCGCGCCGCAAGCGCCGCCTGATGCGCAAGTACAAGATCCAGGAAGTGATCCGCCGCCGGCAGATCATGCTGGTTCAG
GTGGTCAAGGAAGAGCGTGGCAACAAGGGCGCGGCCCTGACCACCTATCTGTCGCTGGCCGGCCGCTACGGCGTCCTGAT
GCCCAACACCGCCCGTGGCGGCGGCATCAGCCGCAAGATCACGGCGGTGACTGACCGCAAGCGCCTGAAGAGCGTCGTCC
AAAGCCTGGACGTGCCGCAAGGCATGGGTCTGATTGTCCGCACAGCCGGCGCCAAGCGCACCAAGGCCGAGATCAAGCGC
GACTATGAGTACCTGCTGCGTCTGTGGGAGAACATCCGCGAGAACACGCTGCACTCGATCGCGCCGGCGCTGATCTACGA
GGAAGAAGACCTCGTCAAACGCGCCATCCGCGACATGTACGACAAGGACCTGGACGGCATCTGGGTCGAGGGCGACGCCG
GCTACAAGGAAGCGCGCGACTTCATGCGCATGCTAATGCCGAGCCAGGCCAAGAAGGTCTTCAACTACCGCGACCCGACC
CCGCTGTTCGTGAAGAACAAGATCGAGGACCATCTGGCCCAGATCTATTCGCCGGTCGTTCCGCTGCGCTCGGGCGGCTA
TCTGGTGATCAACCAGACCGAGGCCCTGGTCGCCATCGACGTCAACTCGGGTAAGGCCACGCGCGAGCGCAACATCGAGG
CCACCGCGCTGAAGACCAACTGCGAAGCGGCCGAGGAAGCCGCCCGTCAGCTGCGTCTGCGCGACCTGGCCGGCCTGATC
GTCATCGACTTCATCGACATGGATGAAGCCAAGAACAACCGCACGGTCGAGAAGGTCCTGAAGGACGCGCTCAAGGACGA
CCGCGCGCGCATCCAGATGGGCAAGATCTCGGGCTTTGGCCTGATGGAGATCAGCCGTCAGCGTCGCCGCACCGGCGTGC
TGGAAGGCACCACCCATGTCTGCGAACACTGCGAAGGCACCGGCCGTGTCCGTTCGGTGGAATCCAGCGCCCTGGCCGCC
CTGCGCGCCGTCGAGGCCGAGGCCCTGAAGGGCTCGGGCAGCGTGATCCTGAAGGTCTCGCGCTCGGTCGGCCTCTATAT
CCTCAACGAAAAGCGCGATTATCTGCAGCGTCTGCTGACCACGCACGGCCTGTTCGTGTCGGTCGTGGTCGATGACAGCC
TGCACGCGGGCGACCAGGAGATCGAGCGCACCGAGCTGGGCGAACGCATCGCCGTGGCCCCGCCGCCCTTCGTCGAGGAA
GACGACGACTTCGATCCGAACGCCTACGACGACGAGGAAGAAGAAGACGACGTCATTCTCGATGACGAGGACGACACCGA
CCGCGAGGACACCGACGACGACGATGCGACGACGCGCAAGTCGGCGCGTGATGACGAGCGCGGCGACCGCAAGGGCCGTC
GCGGGCGTCGCGACCGCAACCGCGGCCGCGGGCGTCGCGACGAGCGGGATGGCGAGACCGAGTCCGAGGACGAGGACGTC
GTGGCCGAAGGCGCGGACGAGGATCGCGGCGAGTTTGGCGATGATGATGAAGGCGGTCGTCGCCGCCGTCGCCGGGGTCG
TCGTGGCGGCCGTCGTGGCGGGCGCGAGGACGGCGATCGTCCGACCGACGCCTTCGTCTGGATCCGTCCGCGGGTGCCCT
TCGGCGAGAACGTCTTCACCTGGCATGATCCGGCTGCGCTGGTCGGCGGTGGCGAGTCGCGTCGTCAGGCGCCCGAGCCG
CGCGTCGATGCCGCTACCGAGGCCGCGCCGCGTCCCGAGCGGGCCGAGCGCGAAGAGCGCCCTGGCCGTGAACGTGGCCG
TCGGGGTCGTGACCGGGGCCGTCGCCAGCGCGACGAGGCGCCGGTCGCCGAGATGACCTCGGTGGAAAGCGCGACTGTCG
AGGCTGCGGAGCCGTTCGAGGCCCCCATCCTGGCGCCGCCGGTAATCGCCGGGCCGCCGGCCGACGTTTGGGTCGAACTG
CCGGAAGTCGAGGAAGCGCCCAAGAAGCCCAAGCGCTCAAGGGCGCGCGGCAAGAAAGCGACTGAAACGTCCGTCGAAGC
GATCGACACCGTCACCGAAGTCGCGGCGGAGGCTCCCGCCCCCGAGACCGCTGAACCCGAAGCCGTCGAGGTCGCTCCGC
CGGCCCCCACGGTCGAGGCTGCGCCTGAGCCGGGACCGGTCGTCGAAGCCGTCGAGGAGGCCCAACCGGCCGAGCCGGAT
CCGAACGAGATCACCGCGCCGCCCGAAAAGCCCCGTCGGGGCTGGTGGCGCCGGTAA

Upstream 100 bases:

>100_bases
CGCAACCGCGCTGACGGTGCGACGGTCGGCGACGCGCGCGCCCTTCATTCATATCGGCGACCGGGCCAAGCGCCCGCAGC
GCGCCGCGGAGACTTCCTTT

Downstream 100 bases:

>100_bases
GGGCGAATGACAACGGCCCGCGCGTCTCCAGATGCGCGGGCCGAATTCTTTCGCGGCGACGGTGCGAACCCTTGTTCCCT
CGCCGTCACGATCCAAGTTA

Product: ribonuclease, Rne/Rng family protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 898; Mature: 897

Protein sequence:

>898_residues
MSKKMLIDAAHAEETRVVVVDGTRVEEFDFESQTRKQLRGNIYLAKVTRVEPSLQAAFIEYGGNRHGFLAFNEIHPDYYQ
IPVADREALMRDDSGDDEDDTPISRRASGGDDEDDVNGGDRAVDDDDDDVEEELARRKRRLMRKYKIQEVIRRRQIMLVQ
VVKEERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITAVTDRKRLKSVVQSLDVPQGMGLIVRTAGAKRTKAEIKR
DYEYLLRLWENIRENTLHSIAPALIYEEEDLVKRAIRDMYDKDLDGIWVEGDAGYKEARDFMRMLMPSQAKKVFNYRDPT
PLFVKNKIEDHLAQIYSPVVPLRSGGYLVINQTEALVAIDVNSGKATRERNIEATALKTNCEAAEEAARQLRLRDLAGLI
VIDFIDMDEAKNNRTVEKVLKDALKDDRARIQMGKISGFGLMEISRQRRRTGVLEGTTHVCEHCEGTGRVRSVESSALAA
LRAVEAEALKGSGSVILKVSRSVGLYILNEKRDYLQRLLTTHGLFVSVVVDDSLHAGDQEIERTELGERIAVAPPPFVEE
DDDFDPNAYDDEEEEDDVILDDEDDTDREDTDDDDATTRKSARDDERGDRKGRRGRRDRNRGRGRRDERDGETESEDEDV
VAEGADEDRGEFGDDDEGGRRRRRRGRRGGRRGGREDGDRPTDAFVWIRPRVPFGENVFTWHDPAALVGGGESRRQAPEP
RVDAATEAAPRPERAEREERPGRERGRRGRDRGRRQRDEAPVAEMTSVESATVEAAEPFEAPILAPPVIAGPPADVWVEL
PEVEEAPKKPKRSRARGKKATETSVEAIDTVTEVAAEAPAPETAEPEAVEVAPPAPTVEAAPEPGPVVEAVEEAQPAEPD
PNEITAPPEKPRRGWWRR

Sequences:

>Translated_898_residues
MSKKMLIDAAHAEETRVVVVDGTRVEEFDFESQTRKQLRGNIYLAKVTRVEPSLQAAFIEYGGNRHGFLAFNEIHPDYYQ
IPVADREALMRDDSGDDEDDTPISRRASGGDDEDDVNGGDRAVDDDDDDVEEELARRKRRLMRKYKIQEVIRRRQIMLVQ
VVKEERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITAVTDRKRLKSVVQSLDVPQGMGLIVRTAGAKRTKAEIKR
DYEYLLRLWENIRENTLHSIAPALIYEEEDLVKRAIRDMYDKDLDGIWVEGDAGYKEARDFMRMLMPSQAKKVFNYRDPT
PLFVKNKIEDHLAQIYSPVVPLRSGGYLVINQTEALVAIDVNSGKATRERNIEATALKTNCEAAEEAARQLRLRDLAGLI
VIDFIDMDEAKNNRTVEKVLKDALKDDRARIQMGKISGFGLMEISRQRRRTGVLEGTTHVCEHCEGTGRVRSVESSALAA
LRAVEAEALKGSGSVILKVSRSVGLYILNEKRDYLQRLLTTHGLFVSVVVDDSLHAGDQEIERTELGERIAVAPPPFVEE
DDDFDPNAYDDEEEEDDVILDDEDDTDREDTDDDDATTRKSARDDERGDRKGRRGRRDRNRGRGRRDERDGETESEDEDV
VAEGADEDRGEFGDDDEGGRRRRRRGRRGGRRGGREDGDRPTDAFVWIRPRVPFGENVFTWHDPAALVGGGESRRQAPEP
RVDAATEAAPRPERAEREERPGRERGRRGRDRGRRQRDEAPVAEMTSVESATVEAAEPFEAPILAPPVIAGPPADVWVEL
PEVEEAPKKPKRSRARGKKATETSVEAIDTVTEVAAEAPAPETAEPEAVEVAPPAPTVEAAPEPGPVVEAVEEAQPAEPD
PNEITAPPEKPRRGWWRR
>Mature_897_residues
SKKMLIDAAHAEETRVVVVDGTRVEEFDFESQTRKQLRGNIYLAKVTRVEPSLQAAFIEYGGNRHGFLAFNEIHPDYYQI
PVADREALMRDDSGDDEDDTPISRRASGGDDEDDVNGGDRAVDDDDDDVEEELARRKRRLMRKYKIQEVIRRRQIMLVQV
VKEERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITAVTDRKRLKSVVQSLDVPQGMGLIVRTAGAKRTKAEIKRD
YEYLLRLWENIRENTLHSIAPALIYEEEDLVKRAIRDMYDKDLDGIWVEGDAGYKEARDFMRMLMPSQAKKVFNYRDPTP
LFVKNKIEDHLAQIYSPVVPLRSGGYLVINQTEALVAIDVNSGKATRERNIEATALKTNCEAAEEAARQLRLRDLAGLIV
IDFIDMDEAKNNRTVEKVLKDALKDDRARIQMGKISGFGLMEISRQRRRTGVLEGTTHVCEHCEGTGRVRSVESSALAAL
RAVEAEALKGSGSVILKVSRSVGLYILNEKRDYLQRLLTTHGLFVSVVVDDSLHAGDQEIERTELGERIAVAPPPFVEED
DDFDPNAYDDEEEEDDVILDDEDDTDREDTDDDDATTRKSARDDERGDRKGRRGRRDRNRGRGRRDERDGETESEDEDVV
AEGADEDRGEFGDDDEGGRRRRRRGRRGGRRGGREDGDRPTDAFVWIRPRVPFGENVFTWHDPAALVGGGESRRQAPEPR
VDAATEAAPRPERAEREERPGRERGRRGRDRGRRQRDEAPVAEMTSVESATVEAAEPFEAPILAPPVIAGPPADVWVELP
EVEEAPKKPKRSRARGKKATETSVEAIDTVTEVAAEAPAPETAEPEAVEVAPPAPTVEAAPEPGPVVEAVEEAQPAEPDP
NEITAPPEKPRRGWWRR

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=549, Percent_Identity=41.3479052823315, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI87082235, Length=338, Percent_Identity=37.2781065088757, Blast_Score=196, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 100694; Mature: 100563

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKMLIDAAHAEETRVVVVDGTRVEEFDFESQTRKQLRGNIYLAKVTRVEPSLQAAFIE
CCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEEECCCHHHHHHHH
YGGNRHGFLAFNEIHPDYYQIPVADREALMRDDSGDDEDDTPISRRASGGDDEDDVNGGD
HCCCCCCEEEEECCCCCEEECCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
RAVDDDDDDVEEELARRKRRLMRKYKIQEVIRRRQIMLVQVVKEERGNKGAALTTYLSLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GRYGVLMPNTARGGGISRKITAVTDRKRLKSVVQSLDVPQGMGLIVRTAGAKRTKAEIKR
CCCCEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHH
DYEYLLRLWENIRENTLHSIAPALIYEEEDLVKRAIRDMYDKDLDGIWVEGDAGYKEARD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
FMRMLMPSQAKKVFNYRDPTPLFVKNKIEDHLAQIYSPVVPLRSGGYLVINQTEALVAID
HHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEE
VNSGKATRERNIEATALKTNCEAAEEAARQLRLRDLAGLIVIDFIDMDEAKNNRTVEKVL
CCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHH
KDALKDDRARIQMGKISGFGLMEISRQRRRTGVLEGTTHVCEHCEGTGRVRSVESSALAA
HHHHHCCHHHEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEHHHHHHHHH
LRAVEAEALKGSGSVILKVSRSVGLYILNEKRDYLQRLLTTHGLFVSVVVDDSLHAGDQE
HHHHHHHHHCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCCHHH
IERTELGERIAVAPPPFVEEDDDFDPNAYDDEEEEDDVILDDEDDTDREDTDDDDATTRK
HHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHH
SARDDERGDRKGRRGRRDRNRGRGRRDERDGETESEDEDVVAEGADEDRGEFGDDDEGGR
HCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHH
RRRRRGRRGGRRGGREDGDRPTDAFVWIRPRVPFGENVFTWHDPAALVGGGESRRQAPEP
HHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCHHEECCCHHHCCCCCC
RVDAATEAAPRPERAEREERPGRERGRRGRDRGRRQRDEAPVAEMTSVESATVEAAEPFE
CCCCHHCCCCCCHHHHHHCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
APILAPPVIAGPPADVWVELPEVEEAPKKPKRSRARGKKATETSVEAIDTVTEVAAEAPA
CCCCCCCCCCCCCHHHEEECCCHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
PETAEPEAVEVAPPAPTVEAAPEPGPVVEAVEEAQPAEPDPNEITAPPEKPRRGWWRR
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKKMLIDAAHAEETRVVVVDGTRVEEFDFESQTRKQLRGNIYLAKVTRVEPSLQAAFIE
CCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEEECCCHHHHHHHH
YGGNRHGFLAFNEIHPDYYQIPVADREALMRDDSGDDEDDTPISRRASGGDDEDDVNGGD
HCCCCCCEEEEECCCCCEEECCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
RAVDDDDDDVEEELARRKRRLMRKYKIQEVIRRRQIMLVQVVKEERGNKGAALTTYLSLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GRYGVLMPNTARGGGISRKITAVTDRKRLKSVVQSLDVPQGMGLIVRTAGAKRTKAEIKR
CCCCEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHH
DYEYLLRLWENIRENTLHSIAPALIYEEEDLVKRAIRDMYDKDLDGIWVEGDAGYKEARD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
FMRMLMPSQAKKVFNYRDPTPLFVKNKIEDHLAQIYSPVVPLRSGGYLVINQTEALVAID
HHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEE
VNSGKATRERNIEATALKTNCEAAEEAARQLRLRDLAGLIVIDFIDMDEAKNNRTVEKVL
CCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHH
KDALKDDRARIQMGKISGFGLMEISRQRRRTGVLEGTTHVCEHCEGTGRVRSVESSALAA
HHHHHCCHHHEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEHHHHHHHHH
LRAVEAEALKGSGSVILKVSRSVGLYILNEKRDYLQRLLTTHGLFVSVVVDDSLHAGDQE
HHHHHHHHHCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCCHHH
IERTELGERIAVAPPPFVEEDDDFDPNAYDDEEEEDDVILDDEDDTDREDTDDDDATTRK
HHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHH
SARDDERGDRKGRRGRRDRNRGRGRRDERDGETESEDEDVVAEGADEDRGEFGDDDEGGR
HCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHH
RRRRRGRRGGRRGGREDGDRPTDAFVWIRPRVPFGENVFTWHDPAALVGGGESRRQAPEP
HHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCHHEECCCHHHCCCCCC
RVDAATEAAPRPERAEREERPGRERGRRGRDRGRRQRDEAPVAEMTSVESATVEAAEPFE
CCCCHHCCCCCCHHHHHHCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
APILAPPVIAGPPADVWVELPEVEEAPKKPKRSRARGKKATETSVEAIDTVTEVAAEAPA
CCCCCCCCCCCCCHHHEEECCCHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
PETAEPEAVEVAPPAPTVEAAPEPGPVVEAVEEAQPAEPDPNEITAPPEKPRRGWWRR
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]