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The map label for this gene is dosC [H]

Identifier: 16126093

GI number: 16126093

Start: 2046644

End: 2047705

Strand: Direct

Name: dosC [H]

Synonym: CC_1850

Alternate gene names: 16126093

Gene position: 2046644-2047705 (Clockwise)

Preceding gene: 16126090

Following gene: 16126094

Centisome position: 50.95

GC content: 67.33

Gene sequence:

>1062_bases
ATGTCGGACGTCGAAACCACGCTGCGCGGTCCAGAGGCCTACAAGATCGCCTCGCGCGCTCTGGAGCTTATGGAACGGCA
TCAAGTCTGGCCGACCGCGCTGAACTTCGAGCTCTGGACGCACTATGTGGCCGACCCGAATGGCGCACTGGCGCGTGAGC
TGACCCGACTGATCTCGCTGGGCGAGCCGATGACCGAACTGGTCAGCGAAGAGCTTGCCGCGACGTTCCTGCCCAAGGCG
CGCCTGAACGAACAGATCCGCGATGCGGGCGACATTCTGTCCAAGGAACTGGAGGCGGTTTCCAAGGCGATCCAGAACGC
CCAGAAGTCCAACGCCGCCTTCGGCCAGGAACTGGCCGGCGCGAGCAAGAGCCTCGACACCCATACCGACGTCGCCTCGA
TCAAGGCGGTGGTCGGCAGCATCGCCGAAGCCACCCGCCGGGTGCACAAGGAAAACCAGTCGCTGGAGGCGCGTCTCGCC
GACTCCACCGCCGAAGTCGAACGCCTGCGCGAACATCTGGAGCAGGTGCGACGCGACGCCACGACCGACGGCCTGACCAA
TCTGGCCAACCGCAAGGCCTTCGACGACGAACTCGACCGCGCCTGCGCCGAGGCCGAGGAAGGTGGCACGACCATCTGCC
TGGCGGTGCTCGATATCGACCACTTCAAGGGCTTCAACGATACCTGGGGCCACCAGACGGGCGACCAGGTCATCCGCTAT
GTCGCCTCAGTCATCGGCCGTGTCGCCGCCCCGCCCCGCTTCGCCGCCCGCTACGGCGGCGAGGAGTTCGCGATGATCTT
CCCCCGCGAAGCCGCCTCGGTGGTGGCGACCACGCTGGAGGAAATCCGCGTCGAGGTGTCGTCGCGCATGCTCAAGCGCC
GCTCGACCAACGAGGACCTGGGCGCGATCACGGTGTCCTCGGGGTTCGCCGAGCGTAAGCCGGGCGAGTCGGGCCACTCG
GTGATGGAACGGGCGGACGCCGCGCTCTACGCCTCCAAGCGTGGTGGCCGCAATCGCGTGACGGCCGCGGAGTCCATGCC
CGGCGCCGCCAACGCCGCCTGA

Upstream 100 bases:

>100_bases
CGTCGTTCGTTCTTAACGCCTCGTAAGAACTCTACAGCTTAGGCTGCAAACCCAAGCGATCGGCATTGCCGCAAGGGCAA
CGTCATCGAGGGAAGAGAAC

Downstream 100 bases:

>100_bases
CCTTATTCAGCAGAAAACCTACCTTTGCGCCGCCGGTCCCTGATCGGCGGCGTTTCTGTTTCTGAAGATTTCGCTAGCCT
TCCCAAGGGGCAAAGGCGCC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSDVETTLRGPEAYKIASRALELMERHQVWPTALNFELWTHYVADPNGALARELTRLISLGEPMTELVSEELAATFLPKA
RLNEQIRDAGDILSKELEAVSKAIQNAQKSNAAFGQELAGASKSLDTHTDVASIKAVVGSIAEATRRVHKENQSLEARLA
DSTAEVERLREHLEQVRRDATTDGLTNLANRKAFDDELDRACAEAEEGGTTICLAVLDIDHFKGFNDTWGHQTGDQVIRY
VASVIGRVAAPPRFAARYGGEEFAMIFPREAASVVATTLEEIRVEVSSRMLKRRSTNEDLGAITVSSGFAERKPGESGHS
VMERADAALYASKRGGRNRVTAAESMPGAANAA

Sequences:

>Translated_353_residues
MSDVETTLRGPEAYKIASRALELMERHQVWPTALNFELWTHYVADPNGALARELTRLISLGEPMTELVSEELAATFLPKA
RLNEQIRDAGDILSKELEAVSKAIQNAQKSNAAFGQELAGASKSLDTHTDVASIKAVVGSIAEATRRVHKENQSLEARLA
DSTAEVERLREHLEQVRRDATTDGLTNLANRKAFDDELDRACAEAEEGGTTICLAVLDIDHFKGFNDTWGHQTGDQVIRY
VASVIGRVAAPPRFAARYGGEEFAMIFPREAASVVATTLEEIRVEVSSRMLKRRSTNEDLGAITVSSGFAERKPGESGHS
VMERADAALYASKRGGRNRVTAAESMPGAANAA
>Mature_352_residues
SDVETTLRGPEAYKIASRALELMERHQVWPTALNFELWTHYVADPNGALARELTRLISLGEPMTELVSEELAATFLPKAR
LNEQIRDAGDILSKELEAVSKAIQNAQKSNAAFGQELAGASKSLDTHTDVASIKAVVGSIAEATRRVHKENQSLEARLAD
STAEVERLREHLEQVRRDATTDGLTNLANRKAFDDELDRACAEAEEGGTTICLAVLDIDHFKGFNDTWGHQTGDQVIRYV
ASVIGRVAAPPRFAARYGGEEFAMIFPREAASVVATTLEEIRVEVSSRMLKRRSTNEDLGAITVSSGFAERKPGESGHSV
MERADAALYASKRGGRNRVTAAESMPGAANAA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=36.4161849710983, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=37.8048780487805, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=32.972972972973, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788381, Length=258, Percent_Identity=28.6821705426357, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=31.0734463276836, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=33.9080459770115, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=200, Percent_Identity=31.5, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787816, Length=184, Percent_Identity=32.6086956521739, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=166, Percent_Identity=28.3132530120482, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787056, Length=202, Percent_Identity=29.7029702970297, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787802, Length=199, Percent_Identity=30.1507537688442, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=31.9767441860465, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788085, Length=188, Percent_Identity=26.063829787234, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081977, Length=181, Percent_Identity=27.6243093922652, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38405; Mature: 38274

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVETTLRGPEAYKIASRALELMERHQVWPTALNFELWTHYVADPNGALARELTRLISL
CCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
GEPMTELVSEELAATFLPKARLNEQIRDAGDILSKELEAVSKAIQNAQKSNAAFGQELAG
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASKSLDTHTDVASIKAVVGSIAEATRRVHKENQSLEARLADSTAEVERLREHLEQVRRDA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TTDGLTNLANRKAFDDELDRACAEAEEGGTTICLAVLDIDHFKGFNDTWGHQTGDQVIRY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCCCCCHHHHHHH
VASVIGRVAAPPRFAARYGGEEFAMIFPREAASVVATTLEEIRVEVSSRMLKRRSTNEDL
HHHHHHHHCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAITVSSGFAERKPGESGHSVMERADAALYASKRGGRNRVTAAESMPGAANAA
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
SDVETTLRGPEAYKIASRALELMERHQVWPTALNFELWTHYVADPNGALARELTRLISL
CCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
GEPMTELVSEELAATFLPKARLNEQIRDAGDILSKELEAVSKAIQNAQKSNAAFGQELAG
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASKSLDTHTDVASIKAVVGSIAEATRRVHKENQSLEARLADSTAEVERLREHLEQVRRDA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TTDGLTNLANRKAFDDELDRACAEAEEGGTTICLAVLDIDHFKGFNDTWGHQTGDQVIRY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCCCCCHHHHHHH
VASVIGRVAAPPRFAARYGGEEFAMIFPREAASVVATTLEEIRVEVSSRMLKRRSTNEDL
HHHHHHHHCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAITVSSGFAERKPGESGHSVMERADAALYASKRGGRNRVTAAESMPGAANAA
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]