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The map label for this gene is dus [H]

Identifier: 16125983

GI number: 16125983

Start: 1911788

End: 1912735

Strand: Direct

Name: dus [H]

Synonym: CC_1739

Alternate gene names: 16125983

Gene position: 1911788-1912735 (Clockwise)

Preceding gene: 16125980

Following gene: 16125984

Centisome position: 47.59

GC content: 68.04

Gene sequence:

>948_bases
ATGACCGGCGTGTCGGATCTGCCCTTCAGAGAAACGGCCTCGGCGCTTGGCGCGCCCTATGTGGCCACCGAAATGGTTGC
GAGCGCCGAGTTCGCCAAAGGGCGTCCTGACGTCGTGCGCCGCGCCGCCGTCGGCGAGGGCTTGCCGCTCACGGTCATCC
AGTTGGTGGGGCGCGACGTCGACTTTATGGCGCAGGGCGCACGCATGGCCCAGGAGGCCGGCGCGGAGATCATCGACCTG
AACTTTGGTTGCCCGGCCAAGGAAGTCGCCGCTGGCGCGGCCTGCGGCTCGGCTCTGATGCGTGAGCCTGACCTCGCCGA
AGCCCTCGTCGCGGCGGCCGTAGAGGCCGTGGATGTTCCGGTGACGGTCAAGATGCGGCTGGGCTGGGATATCGATTGCC
TGAACGCCCCTGACATCGCGCGGCGCGCCGAAGCCGCTGGCGCCAAGGCCGTCACGGTGCATGGGCGCACGCGCAACCAG
TTCTACAAGGGCGTCGCGGATTGGACGGCGGTGGCGGCGGTCAAGCAGGCTGTCTCTGTTCCGGTCCTTGTGAATGGCGA
TATCGTCGACGGTGAAACCGCGCGTCAGGCGCTGGAGCAGTCCGGCGCCGACGGCGTGATGATCGGCCGTGGCGTCTATG
GCCGCCCCTGGATCGCGGGCGCGATCGAGGCGGCGCTGGAGGGACGCGGCTTCCAGGAGCCGGAGGCGGAAGAGCGTCTG
GCCATCGCCATCACCCACTTCCGACGGAGCCTGTCGTTCTATGGCGAGCGGCTGGGCTTGAAGATGTTCCGCAAGCATCT
GGCGTCGTACATCGAGGCTGCGCCTTGGCCGGAAACCGCCGAAGCGCGAAGGGCTGCGCGCGCCAGTCTCTGTCGCCTTG
AAGATCCCGCCGCTGTGGAAAGCGCCCTCCGTACGCTTTGGCTAGCCGAGGGGAGGCAAGCCGCATGA

Upstream 100 bases:

>100_bases
ACAATATGCCTAAAAACGTAGCTGTGGGGATGATTACAAAATGAGCAAAGCGCTCGATATCGGTGGCGTCGAGGTTCCGG
GCCGGGTGTGGATAGCGCCC

Downstream 100 bases:

>100_bases
CCGACCGCGCCCGCGTTCTGGGTGGTGTGGCCACCGAAGCTCTGAAAGCTGCAGCTTTCGACTTGAGCCCCGAGCCTGCA
TTGGTCGTTGATCACGACGG

Product: nitrogen regulation protein Nifr3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTGVSDLPFRETASALGAPYVATEMVASAEFAKGRPDVVRRAAVGEGLPLTVIQLVGRDVDFMAQGARMAQEAGAEIIDL
NFGCPAKEVAAGAACGSALMREPDLAEALVAAAVEAVDVPVTVKMRLGWDIDCLNAPDIARRAEAAGAKAVTVHGRTRNQ
FYKGVADWTAVAAVKQAVSVPVLVNGDIVDGETARQALEQSGADGVMIGRGVYGRPWIAGAIEAALEGRGFQEPEAEERL
AIAITHFRRSLSFYGERLGLKMFRKHLASYIEAAPWPETAEARRAARASLCRLEDPAAVESALRTLWLAEGRQAA

Sequences:

>Translated_315_residues
MTGVSDLPFRETASALGAPYVATEMVASAEFAKGRPDVVRRAAVGEGLPLTVIQLVGRDVDFMAQGARMAQEAGAEIIDL
NFGCPAKEVAAGAACGSALMREPDLAEALVAAAVEAVDVPVTVKMRLGWDIDCLNAPDIARRAEAAGAKAVTVHGRTRNQ
FYKGVADWTAVAAVKQAVSVPVLVNGDIVDGETARQALEQSGADGVMIGRGVYGRPWIAGAIEAALEGRGFQEPEAEERL
AIAITHFRRSLSFYGERLGLKMFRKHLASYIEAAPWPETAEARRAARASLCRLEDPAAVESALRTLWLAEGRQAA
>Mature_314_residues
TGVSDLPFRETASALGAPYVATEMVASAEFAKGRPDVVRRAAVGEGLPLTVIQLVGRDVDFMAQGARMAQEAGAEIIDLN
FGCPAKEVAAGAACGSALMREPDLAEALVAAAVEAVDVPVTVKMRLGWDIDCLNAPDIARRAEAAGAKAVTVHGRTRNQF
YKGVADWTAVAAVKQAVSVPVLVNGDIVDGETARQALEQSGADGVMIGRGVYGRPWIAGAIEAALEGRGFQEPEAEERLA
IAITHFRRSLSFYGERLGLKMFRKHLASYIEAAPWPETAEARRAARASLCRLEDPAAVESALRTLWLAEGRQAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=277, Percent_Identity=29.2418772563177, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI40807366, Length=313, Percent_Identity=31.3099041533546, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI239788462, Length=277, Percent_Identity=29.2418772563177, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=29.004329004329, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923374, Length=205, Percent_Identity=28.2926829268293, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1789660, Length=281, Percent_Identity=40.9252669039146, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1788462, Length=170, Percent_Identity=40.5882352941176, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=32.4200913242009, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=230, Percent_Identity=31.304347826087, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=225, Percent_Identity=32, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=224, Percent_Identity=29.9107142857143, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=25.2427184466019, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=304, Percent_Identity=25.6578947368421, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=32.5991189427313, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=32.5991189427313, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585320, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19921524, Length=144, Percent_Identity=38.1944444444444, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45549423, Length=231, Percent_Identity=29.004329004329, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 33382; Mature: 33251

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVSDLPFRETASALGAPYVATEMVASAEFAKGRPDVVRRAAVGEGLPLTVIQLVGRDV
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCH
DFMAQGARMAQEAGAEIIDLNFGCPAKEVAAGAACGSALMREPDLAEALVAAAVEAVDVP
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
VTVKMRLGWDIDCLNAPDIARRAEAAGAKAVTVHGRTRNQFYKGVADWTAVAAVKQAVSV
EEEEEEECCCEECCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
PVLVNGDIVDGETARQALEQSGADGVMIGRGVYGRPWIAGAIEAALEGRGFQEPEAEERL
CEEECCCEECCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
AIAITHFRRSLSFYGERLGLKMFRKHLASYIEAAPWPETAEARRAARASLCRLEDPAAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
SALRTLWLAEGRQAA
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
TGVSDLPFRETASALGAPYVATEMVASAEFAKGRPDVVRRAAVGEGLPLTVIQLVGRDV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCH
DFMAQGARMAQEAGAEIIDLNFGCPAKEVAAGAACGSALMREPDLAEALVAAAVEAVDVP
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
VTVKMRLGWDIDCLNAPDIARRAEAAGAKAVTVHGRTRNQFYKGVADWTAVAAVKQAVSV
EEEEEEECCCEECCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
PVLVNGDIVDGETARQALEQSGADGVMIGRGVYGRPWIAGAIEAALEGRGFQEPEAEERL
CEEECCCEECCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
AIAITHFRRSLSFYGERLGLKMFRKHLASYIEAAPWPETAEARRAARASLCRLEDPAAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
SALRTLWLAEGRQAA
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]