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The map label for this gene is pdhB [H]

Identifier: 16125971

GI number: 16125971

Start: 1899009

End: 1900361

Strand: Direct

Name: pdhB [H]

Synonym: CC_1727

Alternate gene names: 16125971

Gene position: 1899009-1900361 (Clockwise)

Preceding gene: 16125970

Following gene: 16125972

Centisome position: 47.27

GC content: 66.52

Gene sequence:

>1353_bases
GTGACGGACATCCTGATGCCCGCGCTTTCCCCGACGATGGAGGAAGGCACCCTGGCCAAGTGGCTGGTGAAGGAAGGCGA
CACCATCAAGGCCGGCGACGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCCGTGGACGAGGGCG
TGATCGAAGCCATCCTGGTTCCGGCCGGTAGCGAGAACGTCAAGGTCAACACGCTGATCGCCCGTCTGAAGGGCGACGGC
GAAGCTGCGGCTCCGGCTGTGGCCGCTCCCGTCGCCGAGGCCGCCACGGTTGTCGTCGCCGCTCCGGCCGCTGGCGGACC
GATCTCGGCCGCTTCGACCTTCGCCGATCCGGAAATCCCGACCGGCACGGCGCTGAAGAAGATCACGGTTCGCGACGCCC
TGCGCGACGCGATGGCCGAGGAAATGCGCCGCGATGACCGCGTGTTCCTGATGGGCGAGGAAGTCGCCCAGTATCAGGGC
GCCTACAAGGTCAGCCGTGAGCTGCTGCAGGAGTTCGGCGACCGTCGCGTCATCGATACCCCGATCACCGAGCACGGCTT
CGCAGGCATGGGCGTCGGCGCGGCCATGGCCGGTCTGAAGCCGATCGTCGAGTTCATGACCTGGAACTTCGCCATGCAGG
CGATCGACCACATCATCAACTCGGCGGCCAAGACGCTCTACATGTCGGGCGGCCAGATCAAGTCGTCGATCGTGTTCCGC
GGCCCGAACGGCGCGGCCTCGCGAGTCGGCGCCCAGCACAGCCAGGACTACGCCGCCTGGTACGGCAACGTCCCGGGCCT
GAAGGTCATCGCGCCCTACGACGCCGCTGACGCCAAGGGTCTGCTCAAGGCCGCCATCCGCGATCCGAACCCGGTCGTCT
TCCTCGAGCACGAGATGATGTACGGCCACGAGTTCGACATCCCGGACGTCGAGGACTGGGTCGTGCCGATCGGCAAGGCC
AAGGTCCGTCGCCAAGGCTCGGACGTCACGCTGGTGGCCTACAGCCGCATGGTCGGTTTCGCCCTGAAGGCGGCCGAGGA
GCTGGAAAAGGAAGGCATCGCCGCCGAGGTCGTCGACCTGCGCACGATCCGTCCGATGGACCACGCGACCATCCTGGAAA
GCGTCAAGAAGACCAACCGCCTGGTCACGGTCGAGGAAGGCTGGGGCCCGATGGGCGTCGGCGCAGAGATCGTCGCCCGC
ATCACCGAGTTCGGCTTCGACTACCTGGACGCCCCGCCGCTGCGGGTCTGCCAGGAAGACGTGCCGCTGCCCTATGCGGC
GAACCTGGAAGCGCTCAGCCTGCCTTCGGTCGAGAAGATCGTGAAGGCGGCCAAGGCGGTCTGCTACAAGTAG

Upstream 100 bases:

>100_bases
AAGTGAAGCGCATTGTCGCCGAGGCCGCCGAATTCGCCCGCACGAGCCCCGAGCCCGATCCCTCGGAACTGTATACCGAC
GTCTACCTGGAGGCCGCCGA

Downstream 100 bases:

>100_bases
GCGCGGTGCTTCCGGACCCGGACATCTGTTGGCGCGGCGTCCCCTCGATGATTGATGGCATTCGCTATCCGAATGTCGAC
GTCATCGGGGCGAGCCTGCT

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTDILMPALSPTMEEGTLAKWLVKEGDTIKAGDVIAEIETDKATMEVEAVDEGVIEAILVPAGSENVKVNTLIARLKGDG
EAAAPAVAAPVAEAATVVVAAPAAGGPISAASTFADPEIPTGTALKKITVRDALRDAMAEEMRRDDRVFLMGEEVAQYQG
AYKVSRELLQEFGDRRVIDTPITEHGFAGMGVGAAMAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGQIKSSIVFR
GPNGAASRVGAQHSQDYAAWYGNVPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHEMMYGHEFDIPDVEDWVVPIGKA
KVRRQGSDVTLVAYSRMVGFALKAAEELEKEGIAAEVVDLRTIRPMDHATILESVKKTNRLVTVEEGWGPMGVGAEIVAR
ITEFGFDYLDAPPLRVCQEDVPLPYAANLEALSLPSVEKIVKAAKAVCYK

Sequences:

>Translated_450_residues
MTDILMPALSPTMEEGTLAKWLVKEGDTIKAGDVIAEIETDKATMEVEAVDEGVIEAILVPAGSENVKVNTLIARLKGDG
EAAAPAVAAPVAEAATVVVAAPAAGGPISAASTFADPEIPTGTALKKITVRDALRDAMAEEMRRDDRVFLMGEEVAQYQG
AYKVSRELLQEFGDRRVIDTPITEHGFAGMGVGAAMAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGQIKSSIVFR
GPNGAASRVGAQHSQDYAAWYGNVPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHEMMYGHEFDIPDVEDWVVPIGKA
KVRRQGSDVTLVAYSRMVGFALKAAEELEKEGIAAEVVDLRTIRPMDHATILESVKKTNRLVTVEEGWGPMGVGAEIVAR
ITEFGFDYLDAPPLRVCQEDVPLPYAANLEALSLPSVEKIVKAAKAVCYK
>Mature_449_residues
TDILMPALSPTMEEGTLAKWLVKEGDTIKAGDVIAEIETDKATMEVEAVDEGVIEAILVPAGSENVKVNTLIARLKGDGE
AAAPAVAAPVAEAATVVVAAPAAGGPISAASTFADPEIPTGTALKKITVRDALRDAMAEEMRRDDRVFLMGEEVAQYQGA
YKVSRELLQEFGDRRVIDTPITEHGFAGMGVGAAMAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGQIKSSIVFRG
PNGAASRVGAQHSQDYAAWYGNVPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHEMMYGHEFDIPDVEDWVVPIGKAK
VRRQGSDVTLVAYSRMVGFALKAAEELEKEGIAAEVVDLRTIRPMDHATILESVKKTNRLVTVEEGWGPMGVGAEIVARI
TEFGFDYLDAPPLRVCQEDVPLPYAANLEALSLPSVEKIVKAAKAVCYK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=329, Percent_Identity=61.0942249240122, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI291084858, Length=329, Percent_Identity=58.0547112462006, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI4557353, Length=332, Percent_Identity=34.3373493975904, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI34101272, Length=332, Percent_Identity=34.3373493975904, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI203098816, Length=78, Percent_Identity=51.2820512820513, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI203098753, Length=78, Percent_Identity=51.2820512820513, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI31711992, Length=76, Percent_Identity=47.3684210526316, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI260898739, Length=56, Percent_Identity=57.1428571428571, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI225637463, Length=296, Percent_Identity=27.3648648648649, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI225637461, Length=293, Percent_Identity=26.6211604095563, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI225637459, Length=293, Percent_Identity=26.6211604095563, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI205277463, Length=291, Percent_Identity=25.7731958762887, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI4507521, Length=291, Percent_Identity=25.7731958762887, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=62.5766871165644, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=38.390092879257, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=130, Percent_Identity=37.6923076923077, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539652, Length=259, Percent_Identity=30.5019305019305, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319698, Length=331, Percent_Identity=61.0271903323263, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324258, Length=73, Percent_Identity=56.1643835616438, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321632, Length=104, Percent_Identity=40.3846153846154, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21358145, Length=324, Percent_Identity=65.1234567901235, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24650940, Length=324, Percent_Identity=65.1234567901235, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI160714832, Length=339, Percent_Identity=33.9233038348083, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI160714828, Length=339, Percent_Identity=33.9233038348083, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24650943, Length=109, Percent_Identity=65.1376146788991, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24650945, Length=109, Percent_Identity=65.1376146788991, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI20129315, Length=69, Percent_Identity=49.2753623188406, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48178; Mature: 48047

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDILMPALSPTMEEGTLAKWLVKEGDTIKAGDVIAEIETDKATMEVEAVDEGVIEAILV
CCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEECCCCEEEEHHHHCCCEEEEEE
PAGSENVKVNTLIARLKGDGEAAAPAVAAPVAEAATVVVAAPAAGGPISAASTFADPEIP
CCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCCHHHCCCCCCCC
TGTALKKITVRDALRDAMAEEMRRDDRVFLMGEEVAQYQGAYKVSRELLQEFGDRRVIDT
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PITEHGFAGMGVGAAMAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGQIKSSIVFR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEEE
GPNGAASRVGAQHSQDYAAWYGNVPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHEMM
CCCCHHHHCCCCCCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEHEE
YGHEFDIPDVEDWVVPIGKAKVRRQGSDVTLVAYSRMVGFALKAAEELEKEGIAAEVVDL
CCCCCCCCCCHHHEECCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHH
RTIRPMDHATILESVKKTNRLVTVEEGWGPMGVGAEIVARITEFGFDYLDAPPLRVCQED
HHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHC
VPLPYAANLEALSLPSVEKIVKAAKAVCYK
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDILMPALSPTMEEGTLAKWLVKEGDTIKAGDVIAEIETDKATMEVEAVDEGVIEAILV
CCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEECCCCEEEEHHHHCCCEEEEEE
PAGSENVKVNTLIARLKGDGEAAAPAVAAPVAEAATVVVAAPAAGGPISAASTFADPEIP
CCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCCHHHCCCCCCCC
TGTALKKITVRDALRDAMAEEMRRDDRVFLMGEEVAQYQGAYKVSRELLQEFGDRRVIDT
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PITEHGFAGMGVGAAMAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGQIKSSIVFR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEEE
GPNGAASRVGAQHSQDYAAWYGNVPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHEMM
CCCCHHHHCCCCCCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEHEE
YGHEFDIPDVEDWVVPIGKAKVRRQGSDVTLVAYSRMVGFALKAAEELEKEGIAAEVVDL
CCCCCCCCCCHHHEECCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHH
RTIRPMDHATILESVKKTNRLVTVEEGWGPMGVGAEIVARITEFGFDYLDAPPLRVCQED
HHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHC
VPLPYAANLEALSLPSVEKIVKAAKAVCYK
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]