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The map label for this gene is leuA

Identifier: 16125788

GI number: 16125788

Start: 1704408

End: 1705982

Strand: Direct

Name: leuA

Synonym: CC_1541

Alternate gene names: 16125788

Gene position: 1704408-1705982 (Clockwise)

Preceding gene: 16125787

Following gene: 16125789

Centisome position: 42.43

GC content: 66.92

Gene sequence:

>1575_bases
ATGACCTCCGTACCCGCTGGCGCTATCAGCAAGCGCGACAACGTCGTCGTATTTGACACCACCATGCGCGACGGCGAGCA
GTCGCCCGGCGCCTCCATGTCGCTGGAAGAGAAGCTGGAACTGGCCAAGATCCTCGAGGAGATGGGGGTCGACGTCATCG
AGGCCGGCTTCCCGATCGCCTCGAACGGCGACTTCGAAGCTGTCCGCCAGATCGCCGAACTGATCACCGAGAGCACCGTC
TGCGGCCTGGCGCGCGCTGCCGCCGGCGATATCGACCGCTGCGCCGAGGCCGTTCGTCGCGCCAAGCGCGGCCGCATCCA
CACCTTCATCTCCACCAGCCCCGTACACATGAAGTACAAGCTGCAGATGGAGCCGGACGCGGTTCTGGAAGCGATCACCC
GTTCGGTCAGCCACGCTCGTAACCTGGTCGGTGACGTCGAGTGGTCGGCCGAGGACGCCACCCGCACCGAGCGCGACTTC
CTTAAGCGCTGCGTCGAGGCGGCCATCAAGGCCGGCGCCACCACGATCAACCTGCCCGACACCGTCGGCTACTCGTATCC
GTCGGAGTACGGCGAGCTGTTCCGTGACGTGATCACCAGCGTTCCGGGCGCCGACAAGGCGATCTTCTCGGCCCACTGCC
ACAACGATCTGGGCCTGGCTGTGGCCAACTCGATCGCCGCCATCGAAGGCGGGGCGCGTCAGGTCGAGGTCGCCATCAAC
GGCATCGGCGAGCGCGCCGGCAACGCCGCGCTGGAAGAGATCGTCATGGCCCTGCGCGTGCGCGGCGACCATCTGCCGTA
CGGCACCAGCGTTGATCCCGTGCACATCACCCGCGCCAGCCGCTACGTCTCGGCCATCACCGGCTTCCCGGTGCAGTTCA
ACAAGGCGATCGTCGGCAAGAACGCCTTCGCGCATGAGAGCGGCATCCACCAGGACGGCATGCTGAAGAACGCCGAGACC
TACGAGATCATGAAGCCGGAGGACGTGGGGCAGGGCGCCACGAACCTAGTCATGGGCAAGCACTCGGGCCGTCACGCCTT
CCGCGAGAAGCTGAAGGCTCTGGGTTACGAGCTGGGCCAGAACGCCCTGAACGACGCCTTTGGCCGCTTCAAGGAACTGG
CCGACAAGAAGAAGCACGTCTTCGACGACGACATCGTCGCCCTGGTCGACGACGCTCTGGCCCGTGGCTCGGAGAAGATC
CGCGTCTCGCGCCTGCGCGTGGTGGCCGGCACCGACGGCCAGTCGGCCGAGCTGACCCTGGATATCGACGGCGTCGCCAG
CACGGCCGAGGCGACGGGCGACGGCCCGGTCGACGCAGTGTTCAACGCCATCCACAAGATCGTGCCGCACAGCGCCGCCC
TGCGCCTCTTCCAGGTGCATGCGGTGACCGAAGGCACGGACGCCCAGGCCCAGGTCTCGGTGCGTCTGGAGGAGGACGGC
CGCATCGCCACCGGCGCGGCCGCCGACACCGACACCCTGACCGCCTCGGCCAAGGCCTATGTCAACGCGCTGAACAACCT
CTTCGCCCGCAAGGAGAAGAGCCGGCCTGAAGCGGCGATCGCCAGCGGGTTCTAA

Upstream 100 bases:

>100_bases
CTGGGCGCTCTAGGGGCCGCGCATCGTCGTGGCCGGGCGCACAGGGGATGATTGAACCCGCAGCCCGCACGCAGACACAC
CCCTTTCGACGAGTACTTTC

Downstream 100 bases:

>100_bases
CCCCCAAGCGCTATTGCGACGGACAGGTTGATCCTGTCCGTCAATCTTGCCGGCCCGCCAGGACCGTGACACCCCACGCG
CGCCTTTGGCGTGGGGTCTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTSVPAGAISKRDNVVVFDTTMRDGEQSPGASMSLEEKLELAKILEEMGVDVIEAGFPIASNGDFEAVRQIAELITESTV
CGLARAAAGDIDRCAEAVRRAKRGRIHTFISTSPVHMKYKLQMEPDAVLEAITRSVSHARNLVGDVEWSAEDATRTERDF
LKRCVEAAIKAGATTINLPDTVGYSYPSEYGELFRDVITSVPGADKAIFSAHCHNDLGLAVANSIAAIEGGARQVEVAIN
GIGERAGNAALEEIVMALRVRGDHLPYGTSVDPVHITRASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAET
YEIMKPEDVGQGATNLVMGKHSGRHAFREKLKALGYELGQNALNDAFGRFKELADKKKHVFDDDIVALVDDALARGSEKI
RVSRLRVVAGTDGQSAELTLDIDGVASTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHAVTEGTDAQAQVSVRLEEDG
RIATGAAADTDTLTASAKAYVNALNNLFARKEKSRPEAAIASGF

Sequences:

>Translated_524_residues
MTSVPAGAISKRDNVVVFDTTMRDGEQSPGASMSLEEKLELAKILEEMGVDVIEAGFPIASNGDFEAVRQIAELITESTV
CGLARAAAGDIDRCAEAVRRAKRGRIHTFISTSPVHMKYKLQMEPDAVLEAITRSVSHARNLVGDVEWSAEDATRTERDF
LKRCVEAAIKAGATTINLPDTVGYSYPSEYGELFRDVITSVPGADKAIFSAHCHNDLGLAVANSIAAIEGGARQVEVAIN
GIGERAGNAALEEIVMALRVRGDHLPYGTSVDPVHITRASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAET
YEIMKPEDVGQGATNLVMGKHSGRHAFREKLKALGYELGQNALNDAFGRFKELADKKKHVFDDDIVALVDDALARGSEKI
RVSRLRVVAGTDGQSAELTLDIDGVASTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHAVTEGTDAQAQVSVRLEEDG
RIATGAAADTDTLTASAKAYVNALNNLFARKEKSRPEAAIASGF
>Mature_523_residues
TSVPAGAISKRDNVVVFDTTMRDGEQSPGASMSLEEKLELAKILEEMGVDVIEAGFPIASNGDFEAVRQIAELITESTVC
GLARAAAGDIDRCAEAVRRAKRGRIHTFISTSPVHMKYKLQMEPDAVLEAITRSVSHARNLVGDVEWSAEDATRTERDFL
KRCVEAAIKAGATTINLPDTVGYSYPSEYGELFRDVITSVPGADKAIFSAHCHNDLGLAVANSIAAIEGGARQVEVAING
IGERAGNAALEEIVMALRVRGDHLPYGTSVDPVHITRASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAETY
EIMKPEDVGQGATNLVMGKHSGRHAFREKLKALGYELGQNALNDAFGRFKELADKKKHVFDDDIVALVDDALARGSEKIR
VSRLRVVAGTDGQSAELTLDIDGVASTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHAVTEGTDAQAQVSVRLEEDGR
IATGAAADTDTLTASAKAYVNALNNLFARKEKSRPEAAIASGF

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=47.5806451612903, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=28.6842105263158, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=380, Percent_Identity=28.4210526315789, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=561, Percent_Identity=25.668449197861, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=25.1865671641791, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_CAUCN (B8H607)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002516983.1
- ProteinModelPortal:   B8H607
- SMR:   B8H607
- GeneID:   7331588
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01610
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55991; Mature: 55859

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVPAGAISKRDNVVVFDTTMRDGEQSPGASMSLEEKLELAKILEEMGVDVIEAGFPIA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCC
SNGDFEAVRQIAELITESTVCGLARAAAGDIDRCAEAVRRAKRGRIHTFISTSPVHMKYK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEE
LQMEPDAVLEAITRSVSHARNLVGDVEWSAEDATRTERDFLKRCVEAAIKAGATTINLPD
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCC
TVGYSYPSEYGELFRDVITSVPGADKAIFSAHCHNDLGLAVANSIAAIEGGARQVEVAIN
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEE
GIGERAGNAALEEIVMALRVRGDHLPYGTSVDPVHITRASRYVSAITGFPVQFNKAIVGK
CCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCEEECCHHHCC
NAFAHESGIHQDGMLKNAETYEIMKPEDVGQGATNLVMGKHSGRHAFREKLKALGYELGQ
CCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHH
NALNDAFGRFKELADKKKHVFDDDIVALVDDALARGSEKIRVSRLRVVAGTDGQSAELTL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEE
DIDGVASTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHAVTEGTDAQAQVSVRLEEDG
EECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEECCCC
RIATGAAADTDTLTASAKAYVNALNNLFARKEKSRPEAAIASGF
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
TSVPAGAISKRDNVVVFDTTMRDGEQSPGASMSLEEKLELAKILEEMGVDVIEAGFPIA
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCC
SNGDFEAVRQIAELITESTVCGLARAAAGDIDRCAEAVRRAKRGRIHTFISTSPVHMKYK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEE
LQMEPDAVLEAITRSVSHARNLVGDVEWSAEDATRTERDFLKRCVEAAIKAGATTINLPD
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCC
TVGYSYPSEYGELFRDVITSVPGADKAIFSAHCHNDLGLAVANSIAAIEGGARQVEVAIN
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEE
GIGERAGNAALEEIVMALRVRGDHLPYGTSVDPVHITRASRYVSAITGFPVQFNKAIVGK
CCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCEEECCHHHCC
NAFAHESGIHQDGMLKNAETYEIMKPEDVGQGATNLVMGKHSGRHAFREKLKALGYELGQ
CCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHH
NALNDAFGRFKELADKKKHVFDDDIVALVDDALARGSEKIRVSRLRVVAGTDGQSAELTL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEE
DIDGVASTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHAVTEGTDAQAQVSVRLEEDG
EECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEECCCC
RIATGAAADTDTLTASAKAYVNALNNLFARKEKSRPEAAIASGF
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA