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The map label for this gene is ligA

Identifier: 16125769

GI number: 16125769

Start: 1683405

End: 1685756

Strand: Direct

Name: ligA

Synonym: CC_1522

Alternate gene names: 16125769

Gene position: 1683405-1685756 (Clockwise)

Preceding gene: 16125767

Following gene: 16125770

Centisome position: 41.91

GC content: 69.22

Gene sequence:

>2352_bases
GTGTCCCAGATCCCCGTCGCCGACCTCACCGAAGCCCAGGCCGTCGAGGACCTGGAGCGTCTGGCCGACCTTCTGGCGAC
GCACGACATCGCCTATCACCAAGAGGACAATCCGACGGTCAGCGACGCCGAGTACGACGCGCTGAAGCGGCGAAACCTCG
ATATCGAGACGCGCTTCCCGCATCTGGTGCGCGACAACTCCCCGTCGATGCGGGTCGGCGCGACCCGGGCCGAGCAGTTC
GCGCCGGTGGAGCACGGCGTGCCGATGCTCAGCCTCGACAACGCCTTTTCGAACGATGAGGCGATCGAGTTTGACGCCCG
CGTCCGCCGCTTCCTGCGCATCTCGCCGTCCGAGACGGTCGCCTACACCGCCGAGCCCAAGATCGACGGCCTGTCGGCCT
CGCTGCGCTATGAGAAGGGCGTGCTGGTCCAGGGCGCGACGCGGGGCGATGGGCGCGTGGGCGAGGACGTCACCGCCAAT
CTGCGGACCATCGCCGACATTCCGCACCGCCTGAAGGGCTCGGGTTGGCCCGACGTGATCGAGGTGCGCGGCGAGGTCTA
TGTCGAGCTTGCGGCCTTCGCCGCCTTCAACAAGGCCGCCGAGGAGGCCGGTCAGCGCACCTATGCCAATCCCCGCAACT
TCGCGGCGGGCTCGCTGCGCCAGATCGACCCGAAGATCAGCGCCCAGCGGCCGCTGCGGTTCTTCGGCTATGCCTGGGGG
CTGGTGTCGGAGGGCTTCGCCGACAGCCAGTGGGGCGCGCTGGAAAGGCTGGCTGAATGGGGCTTCGTGACCACAGCGCC
GCCCGCCCAGCGCGTGCTGAACGCGCAAGGTCTGCTCGACATCTACGCCCAGTTCGAGGTCCTGCGACCGACCCTGGGCT
TCGACATCGACGGCGTGGTCTACAAGGTCGATGACCTGGAGCTTCAGCGCCGTCTGGGCTTTGTCTCGCGCTCGCCGCGC
TGGGCCATCGCGCGGAAGTTCCCGGCCCAACGCGCGCGGACCGTGCTGGAAGCCATCGATCTCCAGGTCGGCCGCACCGG
CGCGATCACCCCCGTGGCGCGGCTGAAGCCCGTGACGGTCGGCGGCGTGTCGGTGACCAACGCCACCTTGCACAACGGCG
ACGAGATCGCGCGGCTGGACGTGCGGGTCGGCGACACCGTGGTGATCCAGCGGGCCGGTGACGTGATCCCGCAGATCGTC
GAGGTGGCCCTGGACGCGCGGCCAGATCCCGCGCCGCCACCGTACGAATTCCCCCATGTCTGCCCATGCCCGCTGCAGAC
GCCGCTGGCGCGCGAGGTCACCGCATCGGGCCAGGAGTCGGTGGTGCGCCGCTGCACGGGCGAGTTCGCCTGTCCGTTCC
AGCGGGTCGAGCACCTGCGCCACTTCGTCTCCCGCCGGGCGTTCGACATCGAGGGGCTGGGCGAAAAGCAGCTTCAGGCC
TTTTTCGAGGAGGGCTGGATCACCGAGCCCGCCGACATCTTCAAGCTGGCCCGCGACGCGGAAAAGCTGGCCGTCCTGCG
CGAACGCGAGGGCTATGGCGAGACCTCGGTGGCCAATCTGGTCAAGGGGATCGAGGCGCGGCGCACCATCGGCATGGATC
GGATGATCTATGGCCTGGGCGCGCGCGACATCGGCGAGACCACCTCCACGGTGCTGGCCCGCAACTTCGACCGCTTCGAG
GACCTGCAGGCCGCCGCCGAGGCCGCCGCGCGGGCGCTGCCCGGCGAGACCTATCTGGAGCTCTCGACCGCGCCCGGCGT
CGGACCCAAGGCGCTGGACATGCTGGTCGAGGCGGGGAAGGGCGGTGTGGTCGCCGATCCGTGGCCGCAGACTGATGACC
TGGAACTCAAGATCGGCCACGCGGTCCCGAAGCTCACCAAGCCGGCGCGCGCGGCCTTGGCGCAGCGCTACGGAACCTGG
GACGCCTTCGCCGACGGGCTTGTCGCCGCCGCCTCGGGGGCGCCGGGCGACGACTATCTGCATCTGGCCGCCATCGATGG
CGTCGGACCGGTCGCGGCGCAGTCCCTGGCGCGGTTCTTCGCCGAGGATCATAACCGGCAGAAGGTCGCCAATCTCGTCG
CCGAGCTCGACATTCAGCCGGTGGCCAAGCCCAAGACCGACACCGCCGTCGCCGGCAAGACCATCGTCTTCACCGGCTCG
CTGGAGAAGATGACCCGCGACGAGGCCAAGGCTCAGGCCGAAGGGCTGGGCGCCAAGGTCGCGTCTTCGGTGTCGAAGAA
GACCGATCTGGTGGTTGCTGGACCCGGCGCCGGCTCCAAGCTGAAGACCGCCACGGACCTGGGCATCCAGGTGATGACCG
AGGACGAGTGGCTGGAGCTTGTCGCCGGATGA

Upstream 100 bases:

>100_bases
GCGGGCCAAGTTTCGCCTGGCGGGCGAGCGCATCGGCGACTCCCCCCGCCGGAGCCGCGTTGCTAACCTGCCTGCGTTCC
CCGCCGTTCGGATTCGCCCC

Downstream 100 bases:

>100_bases
AGGTCATCGCCATCGACTTCGAGACGGCCAGCGAGCAGCGCGCCAGCCCTTGCTCCATCGGCCTAGCCTGGATCGAGGAC
GGCGAGGTCGCGCGCGTCGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MSQIPVADLTEAQAVEDLERLADLLATHDIAYHQEDNPTVSDAEYDALKRRNLDIETRFPHLVRDNSPSMRVGATRAEQF
APVEHGVPMLSLDNAFSNDEAIEFDARVRRFLRISPSETVAYTAEPKIDGLSASLRYEKGVLVQGATRGDGRVGEDVTAN
LRTIADIPHRLKGSGWPDVIEVRGEVYVELAAFAAFNKAAEEAGQRTYANPRNFAAGSLRQIDPKISAQRPLRFFGYAWG
LVSEGFADSQWGALERLAEWGFVTTAPPAQRVLNAQGLLDIYAQFEVLRPTLGFDIDGVVYKVDDLELQRRLGFVSRSPR
WAIARKFPAQRARTVLEAIDLQVGRTGAITPVARLKPVTVGGVSVTNATLHNGDEIARLDVRVGDTVVIQRAGDVIPQIV
EVALDARPDPAPPPYEFPHVCPCPLQTPLAREVTASGQESVVRRCTGEFACPFQRVEHLRHFVSRRAFDIEGLGEKQLQA
FFEEGWITEPADIFKLARDAEKLAVLREREGYGETSVANLVKGIEARRTIGMDRMIYGLGARDIGETTSTVLARNFDRFE
DLQAAAEAAARALPGETYLELSTAPGVGPKALDMLVEAGKGGVVADPWPQTDDLELKIGHAVPKLTKPARAALAQRYGTW
DAFADGLVAAASGAPGDDYLHLAAIDGVGPVAAQSLARFFAEDHNRQKVANLVAELDIQPVAKPKTDTAVAGKTIVFTGS
LEKMTRDEAKAQAEGLGAKVASSVSKKTDLVVAGPGAGSKLKTATDLGIQVMTEDEWLELVAG

Sequences:

>Translated_783_residues
MSQIPVADLTEAQAVEDLERLADLLATHDIAYHQEDNPTVSDAEYDALKRRNLDIETRFPHLVRDNSPSMRVGATRAEQF
APVEHGVPMLSLDNAFSNDEAIEFDARVRRFLRISPSETVAYTAEPKIDGLSASLRYEKGVLVQGATRGDGRVGEDVTAN
LRTIADIPHRLKGSGWPDVIEVRGEVYVELAAFAAFNKAAEEAGQRTYANPRNFAAGSLRQIDPKISAQRPLRFFGYAWG
LVSEGFADSQWGALERLAEWGFVTTAPPAQRVLNAQGLLDIYAQFEVLRPTLGFDIDGVVYKVDDLELQRRLGFVSRSPR
WAIARKFPAQRARTVLEAIDLQVGRTGAITPVARLKPVTVGGVSVTNATLHNGDEIARLDVRVGDTVVIQRAGDVIPQIV
EVALDARPDPAPPPYEFPHVCPCPLQTPLAREVTASGQESVVRRCTGEFACPFQRVEHLRHFVSRRAFDIEGLGEKQLQA
FFEEGWITEPADIFKLARDAEKLAVLREREGYGETSVANLVKGIEARRTIGMDRMIYGLGARDIGETTSTVLARNFDRFE
DLQAAAEAAARALPGETYLELSTAPGVGPKALDMLVEAGKGGVVADPWPQTDDLELKIGHAVPKLTKPARAALAQRYGTW
DAFADGLVAAASGAPGDDYLHLAAIDGVGPVAAQSLARFFAEDHNRQKVANLVAELDIQPVAKPKTDTAVAGKTIVFTGS
LEKMTRDEAKAQAEGLGAKVASSVSKKTDLVVAGPGAGSKLKTATDLGIQVMTEDEWLELVAG
>Mature_782_residues
SQIPVADLTEAQAVEDLERLADLLATHDIAYHQEDNPTVSDAEYDALKRRNLDIETRFPHLVRDNSPSMRVGATRAEQFA
PVEHGVPMLSLDNAFSNDEAIEFDARVRRFLRISPSETVAYTAEPKIDGLSASLRYEKGVLVQGATRGDGRVGEDVTANL
RTIADIPHRLKGSGWPDVIEVRGEVYVELAAFAAFNKAAEEAGQRTYANPRNFAAGSLRQIDPKISAQRPLRFFGYAWGL
VSEGFADSQWGALERLAEWGFVTTAPPAQRVLNAQGLLDIYAQFEVLRPTLGFDIDGVVYKVDDLELQRRLGFVSRSPRW
AIARKFPAQRARTVLEAIDLQVGRTGAITPVARLKPVTVGGVSVTNATLHNGDEIARLDVRVGDTVVIQRAGDVIPQIVE
VALDARPDPAPPPYEFPHVCPCPLQTPLAREVTASGQESVVRRCTGEFACPFQRVEHLRHFVSRRAFDIEGLGEKQLQAF
FEEGWITEPADIFKLARDAEKLAVLREREGYGETSVANLVKGIEARRTIGMDRMIYGLGARDIGETTSTVLARNFDRFED
LQAAAEAAARALPGETYLELSTAPGVGPKALDMLVEAGKGGVVADPWPQTDDLELKIGHAVPKLTKPARAALAQRYGTWD
AFADGLVAAASGAPGDDYLHLAAIDGVGPVAAQSLARFFAEDHNRQKVANLVAELDIQPVAKPKTDTAVAGKTIVFTGSL
EKMTRDEAKAQAEGLGAKVASSVSKKTDLVVAGPGAGSKLKTATDLGIQVMTEDEWLELVAG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=567, Percent_Identity=46.5608465608466, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87082305, Length=425, Percent_Identity=26.1176470588235, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CAUCN (B8H5Y7)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002516963.1
- ProteinModelPortal:   B8H5Y7
- GeneID:   7331568
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_01590
- OMA:   IKHFASR
- ProtClustDB:   CLSK2516837
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 84902; Mature: 84771

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 185-185 BINDING 302-302 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIPVADLTEAQAVEDLERLADLLATHDIAYHQEDNPTVSDAEYDALKRRNLDIETRFP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHCCCCCCCCCC
HLVRDNSPSMRVGATRAEQFAPVEHGVPMLSLDNAFSNDEAIEFDARVRRFLRISPSETV
HHHCCCCCCEEECCCHHHHCCCHHHCCCEEEECCCCCCCCCEEHHHHHHHHHCCCCCCCE
AYTAEPKIDGLSASLRYEKGVLVQGATRGDGRVGEDVTANLRTIADIPHRLKGSGWPDVI
EEECCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHCHHHHCCCCCCHHH
EVRGEVYVELAAFAAFNKAAEEAGQRTYANPRNFAAGSLRQIDPKISAQRPLRFFGYAWG
HCCCHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
LVSEGFADSQWGALERLAEWGFVTTAPPAQRVLNAQGLLDIYAQFEVLRPTLGFDIDGVV
HHHCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YKVDDLELQRRLGFVSRSPRWAIARKFPAQRARTVLEAIDLQVGRTGAITPVARLKPVTV
EEECHHHHHHHHCHHCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEE
GGVSVTNATLHNGDEIARLDVRVGDTVVIQRAGDVIPQIVEVALDARPDPAPPPYEFPHV
CCEEEECCEECCCCCEEEEEEECCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
CPCPLQTPLAREVTASGQESVVRRCTGEFACPFQRVEHLRHFVSRRAFDIEGLGEKQLQA
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FFEEGWITEPADIFKLARDAEKLAVLREREGYGETSVANLVKGIEARRTIGMDRMIYGLG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCC
ARDIGETTSTVLARNFDRFEDLQAAAEAAARALPGETYLELSTAPGVGPKALDMLVEAGK
CCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCC
GGVVADPWPQTDDLELKIGHAVPKLTKPARAALAQRYGTWDAFADGLVAAASGAPGDDYL
CCEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHCCHHEECCCCCCCCEE
HLAAIDGVGPVAAQSLARFFAEDHNRQKVANLVAELDIQPVAKPKTDTAVAGKTIVFTGS
EEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
LEKMTRDEAKAQAEGLGAKVASSVSKKTDLVVAGPGAGSKLKTATDLGIQVMTEDEWLEL
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCEEEEECHHHHHH
VAG
HCC
>Mature Secondary Structure 
SQIPVADLTEAQAVEDLERLADLLATHDIAYHQEDNPTVSDAEYDALKRRNLDIETRFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHCCCCCCCCCC
HLVRDNSPSMRVGATRAEQFAPVEHGVPMLSLDNAFSNDEAIEFDARVRRFLRISPSETV
HHHCCCCCCEEECCCHHHHCCCHHHCCCEEEECCCCCCCCCEEHHHHHHHHHCCCCCCCE
AYTAEPKIDGLSASLRYEKGVLVQGATRGDGRVGEDVTANLRTIADIPHRLKGSGWPDVI
EEECCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHCHHHHCCCCCCHHH
EVRGEVYVELAAFAAFNKAAEEAGQRTYANPRNFAAGSLRQIDPKISAQRPLRFFGYAWG
HCCCHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
LVSEGFADSQWGALERLAEWGFVTTAPPAQRVLNAQGLLDIYAQFEVLRPTLGFDIDGVV
HHHCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YKVDDLELQRRLGFVSRSPRWAIARKFPAQRARTVLEAIDLQVGRTGAITPVARLKPVTV
EEECHHHHHHHHCHHCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEE
GGVSVTNATLHNGDEIARLDVRVGDTVVIQRAGDVIPQIVEVALDARPDPAPPPYEFPHV
CCEEEECCEECCCCCEEEEEEECCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
CPCPLQTPLAREVTASGQESVVRRCTGEFACPFQRVEHLRHFVSRRAFDIEGLGEKQLQA
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FFEEGWITEPADIFKLARDAEKLAVLREREGYGETSVANLVKGIEARRTIGMDRMIYGLG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCC
ARDIGETTSTVLARNFDRFEDLQAAAEAAARALPGETYLELSTAPGVGPKALDMLVEAGK
CCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCC
GGVVADPWPQTDDLELKIGHAVPKLTKPARAALAQRYGTWDAFADGLVAAASGAPGDDYL
CCEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHCCHHEECCCCCCCCEE
HLAAIDGVGPVAAQSLARFFAEDHNRQKVANLVAELDIQPVAKPKTDTAVAGKTIVFTGS
EEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
LEKMTRDEAKAQAEGLGAKVASSVSKKTDLVVAGPGAGSKLKTATDLGIQVMTEDEWLEL
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCEEEEECHHHHHH
VAG
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA