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The map label for this gene is tuf1 [H]

Identifier: 16125489

GI number: 16125489

Start: 1397512

End: 1398702

Strand: Direct

Name: tuf1 [H]

Synonym: CC_1240

Alternate gene names: 16125489

Gene position: 1397512-1398702 (Clockwise)

Preceding gene: 16125487

Following gene: 16125490

Centisome position: 34.79

GC content: 62.97

Gene sequence:

>1191_bases
ATGGCCAAGGAAAAGTTCGAACGTACTAAGCCGCACTGCAACATCGGCACCATTGGTCACGTTGACCATGGCAAGACGAC
GCTGACCGCCGCGATCACGATGACGCTGGCGAAGTCGGGCGGCGCGACGGCCAAGAAGTACGACGAAATCGACGCGGCGC
CGGAAGAAAAGGCCCGCGGCATCACGATCAACACCGCGCACGTGGAATATGAGACGGCCAACCGTCACTACGCCCACGTC
GACTGCCCCGGCCACGCCGACTACGTGAAGAACATGATCACGGGCGCGGCGCAGATGGACGGCGCGATCCTGGTGGTTTC
GGCCGCTGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTGGCCCGTCAGGTCGGCGTGCCGGCTCTGGTCGTGT
TCATGAACAAGGTCGACATGGTCGACGACGAAGAGCTGCTGGAGCTCGTCGAAATGGAAGTGCGCGAGCTGCTGAGCAGC
TACCAGTTCCCGGGCGACGACATTCCGATCACCAAGGGTTCGGCTCTGGCCGCCGTTGAAGGTCGTGACCCGCAAATCGG
CGAAGAGAAGATCCTCGAGCTGATGGCTTCGGTCGACGCCTACATCCCGCAGCCGGAACGCCCCGTGGACATGCCGTTCC
TGATGCCGGTCGAAGACGTGTTCTCGATCTCGGGTCGCGGTACGGTCGTCACCGGTCGCGTCGAGCGCGGCATCGTGAAG
GTCGGTGAAGAAGTCGAAATCGTCGGCATCCGTCCGGTTCAGAAGACGACCTGCACGGGCGTTGAAATGTTCCGCAAGCT
GCTGGACCAAGGTCAAGCCGGCGACAACGTGGGCGTGCTGCTGCGCGGCACCAAGCGTGAAGACGTCGAGCGCGGCCAGG
TGCTGTGCAAGCCGGGTTCGATCACCCCGCACACCAAGTTCGTGGCCGAAGCCTACATCCTGACCAAGGAAGAAGGCGGC
CGTCACACCCCGTTCTTCACCAACTACCGTCCGCAGTTCTACTTCCGCACGACGGACGTGACGGGCATCATCAAGCTGCG
CGAAGGCGTGGAAATGATCATGCCGGGCGACAACGCCGAGCTGGACGTCGAGCTGATCACCCCGATCGCCATGGAAGAGA
AGCTGCGCTTCGCCATCCGTGAAGGCGGCCGCACCGTCGGCGCCGGCGTGGTTGCGAAGATCGTTGAATAA

Upstream 100 bases:

>100_bases
GCCTTCCAAGCTGAGGATGCGGGTTCGATTCCCGCTACCCGCTCCAGCTCCCTGAAGAGTTATTCCCAACAACCCAGCCG
CCGGCGCGAAGGTAGAGACG

Downstream 100 bases:

>100_bases
TCTCGAACGTTCTTAGCTTCGGCTGACAATGCGAAGGCCCCGGAGAGCAATCTCCGGGGCCTTTTGCTTGGAGAAAAAAC
ACGGGATGATCGGTGATGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITMTLAKSGGATAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDEELLELVEMEVRELLSS
YQFPGDDIPITKGSALAAVEGRDPQIGEEKILELMASVDAYIPQPERPVDMPFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEVEIVGIRPVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTKFVAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGIIKLREGVEMIMPGDNAELDVELITPIAMEEKLRFAIREGGRTVGAGVVAKIVE

Sequences:

>Translated_396_residues
MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITMTLAKSGGATAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDEELLELVEMEVRELLSS
YQFPGDDIPITKGSALAAVEGRDPQIGEEKILELMASVDAYIPQPERPVDMPFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEVEIVGIRPVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTKFVAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGIIKLREGVEMIMPGDNAELDVELITPIAMEEKLRFAIREGGRTVGAGVVAKIVE
>Mature_395_residues
AKEKFERTKPHCNIGTIGHVDHGKTTLTAAITMTLAKSGGATAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDEELLELVEMEVRELLSSY
QFPGDDIPITKGSALAAVEGRDPQIGEEKILELMASVDAYIPQPERPVDMPFLMPVEDVFSISGRGTVVTGRVERGIVKV
GEEVEIVGIRPVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTKFVAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGIIKLREGVEMIMPGDNAELDVELITPIAMEEKLRFAIREGGRTVGAGVVAKIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.6142131979695, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=29.7052154195011, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=29.7052154195011, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=29.3986636971047, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503475, Length=449, Percent_Identity=28.7305122494432, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI194018522, Length=349, Percent_Identity=29.2263610315186, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=349, Percent_Identity=29.2263610315186, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194097354, Length=349, Percent_Identity=29.2263610315186, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI46094014, Length=326, Percent_Identity=29.4478527607362, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI54607086, Length=327, Percent_Identity=28.4403669724771, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=27.2388059701493, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI82546879, Length=421, Percent_Identity=23.5154394299287, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=35.1145038167939, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=35.7798165137615, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=35.7798165137615, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=35.7798165137615, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=78.2278481012658, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=78.2278481012658, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI2367247, Length=386, Percent_Identity=31.8652849740933, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=33.195020746888, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789108, Length=321, Percent_Identity=29.595015576324, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=30.8219178082192, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=54.25, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=330, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=29.490022172949, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=29.490022172949, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.8538812785388, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=29.5194508009153, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=29.5194508009153, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=27.0509977827051, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=29, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=161, Percent_Identity=38.5093167701863, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=304, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.1512605042017, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.4141414141414, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=29.0249433106576, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=29.0249433106576, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=26.0579064587973, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=28.0701754385965, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=275, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.7310924369748, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=30, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=59.8971722365039, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=59.8971722365039, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=49.2227979274611, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=29.6052631578947, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=29.6052631578947, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=29.6052631578947, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=29.6052631578947, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=29.3859649122807, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=29.3859649122807, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45550900, Length=446, Percent_Identity=27.3542600896861, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137380, Length=326, Percent_Identity=30.9815950920245, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.2527881040892, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.2527881040892, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78706572, Length=134, Percent_Identity=33.5820895522388, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=33.5365853658537, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43340; Mature: 43209

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITMTLAKSGGATAKKYDEIDAAPEEKARG
CCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFMNKVDMVDDEELLELVEMEVRELLSSYQFPGDDIPITKGSALAAVE
HHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GRDPQIGEEKILELMASVDAYIPQPERPVDMPFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECHHHCCHHH
VGEEVEIVGIRPVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
ITPHTKFVAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIIKLREGVEMIMPGDNAE
CCCCHHHEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEEECCCCEEEECCCCCE
LDVELITPIAMEEKLRFAIREGGRTVGAGVVAKIVE
EEEEEECCHHHHHHHHHHHHHCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHCNIGTIGHVDHGKTTLTAAITMTLAKSGGATAKKYDEIDAAPEEKARG
CCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFMNKVDMVDDEELLELVEMEVRELLSSYQFPGDDIPITKGSALAAVE
HHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GRDPQIGEEKILELMASVDAYIPQPERPVDMPFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECHHHCCHHH
VGEEVEIVGIRPVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
ITPHTKFVAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIIKLREGVEMIMPGDNAE
CCCCHHHEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEEECCCCEEEECCCCCE
LDVELITPIAMEEKLRFAIREGGRTVGAGVVAKIVE
EEEEEECCHHHHHHHHHHHHHCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA