Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 16125467

GI number: 16125467

Start: 1375600

End: 1376634

Strand: Direct

Name: folD [H]

Synonym: CC_1217

Alternate gene names: 16125467

Gene position: 1375600-1376634 (Clockwise)

Preceding gene: 16125463

Following gene: 16125468

Centisome position: 34.24

GC content: 69.28

Gene sequence:

>1035_bases
ATGGGCGGCGGGAGTCGGGAAGACTTGTGTCATGGCGCGAGGACACGCCGGGGCGGGGAGGCGGTCAAGTCCCCAGGCGG
CCGAGCGCCGTTGCGAGTCTCGCGCCCAGAGGCTAGGGACGGGCAACGCAGGAGAACCCCTATGGCGCAGGCGAAACTCA
TCGACGGCAAGGCCTTCGCGGCCGATCTGAGGGCCAAGATCGCCGAGGAGGTCGCCGCCCTGAAGGCTGAGCATGGCGTG
ACGCCGGGCCTGGCGGTCGTGTTGGTCGGCGAGGATCCGGCCAGCCAGGTCTATGTCCGCAACAAGGGCGAGCAGACCAC
GGCGGCGGGCATGTATTCCGAGACCCATCGCCTGCCGGAAACCACCACTCAGGACGAGCTCCTGGCCGTCGTCGCCAAGC
TGAACGCCGATCCCAAGATCCACGGCGTGCTGGTGCAGTTTCCGGTGCCGCCGCACATCAGCCAGATGGACGTCGTGGCC
GCCCTGTCGCCCGACAAGGACGTCGACGGCCTGACCGTGACCAACGCCGGCCGCTTGGCCAGCGGCCTGCCGGCCCTGGC
GCCCTGCACGCCGACCGGCTGCATGATGCTGATCAGGGACGCGATCGGCGACCTGAAGGGCAAGACCGCCGTCGTCATCG
GCCGCTCGAACCTGATGGGCAAGCCGATGGCCCAGATGCTGCTGGCCGCCGACTGCACCGTCACCATCGCCCACTCGCGC
AGCCAGGATCTGCCCTCGATCGTGCGTCAGGCCGACATCGTGGTGGCCGCCGTGGGCCGGGCCGAGATGGTCAAGGCTGA
CTGGGTGAAGCCCGGCGCCGTGGTCATTGACGTCGGTATCACCCGCTTCCCGGCCCGCGATCCCGAAGCCGCCGCCGCCG
GCAAGACCCGGCTTGTCGGCGACGTCGCCTTCGACGAGGTTAGGGAGGTCGCCGGCGCGATTACGCCGGTTCCGGGGGGC
GTTGGACCGATGACGATTGCTTGCTTGCTGGCCAATACGCTGACGGCGGCCAAGCGCCTGTCGGGGATCGCCTGA

Upstream 100 bases:

>100_bases
CGACATGGCCCAGGATCGCGCCGTCGATCGGGCCGCGCACAGGCTCTCCGGCGTCGGCGGGCAGCGGCGGCGCGCCCAGC
TTGGCCAGGATCTCGCGGGC

Downstream 100 bases:

>100_bases
TGCGCCGGGGGCTGGTTTTCGTCGCCGCCGCCCTCGTCGTTTCGGCCTGCGGGGCCAAGGTCCCGCCGCCGCCCACGCCC
GAACAGGCCCTGGCGCTGCG

Product: FolD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MGGGSREDLCHGARTRRGGEAVKSPGGRAPLRVSRPEARDGQRRRTPMAQAKLIDGKAFAADLRAKIAEEVAALKAEHGV
TPGLAVVLVGEDPASQVYVRNKGEQTTAAGMYSETHRLPETTTQDELLAVVAKLNADPKIHGVLVQFPVPPHISQMDVVA
ALSPDKDVDGLTVTNAGRLASGLPALAPCTPTGCMMLIRDAIGDLKGKTAVVIGRSNLMGKPMAQMLLAADCTVTIAHSR
SQDLPSIVRQADIVVAAVGRAEMVKADWVKPGAVVIDVGITRFPARDPEAAAAGKTRLVGDVAFDEVREVAGAITPVPGG
VGPMTIACLLANTLTAAKRLSGIA

Sequences:

>Translated_344_residues
MGGGSREDLCHGARTRRGGEAVKSPGGRAPLRVSRPEARDGQRRRTPMAQAKLIDGKAFAADLRAKIAEEVAALKAEHGV
TPGLAVVLVGEDPASQVYVRNKGEQTTAAGMYSETHRLPETTTQDELLAVVAKLNADPKIHGVLVQFPVPPHISQMDVVA
ALSPDKDVDGLTVTNAGRLASGLPALAPCTPTGCMMLIRDAIGDLKGKTAVVIGRSNLMGKPMAQMLLAADCTVTIAHSR
SQDLPSIVRQADIVVAAVGRAEMVKADWVKPGAVVIDVGITRFPARDPEAAAAGKTRLVGDVAFDEVREVAGAITPVPGG
VGPMTIACLLANTLTAAKRLSGIA
>Mature_343_residues
GGGSREDLCHGARTRRGGEAVKSPGGRAPLRVSRPEARDGQRRRTPMAQAKLIDGKAFAADLRAKIAEEVAALKAEHGVT
PGLAVVLVGEDPASQVYVRNKGEQTTAAGMYSETHRLPETTTQDELLAVVAKLNADPKIHGVLVQFPVPPHISQMDVVAA
LSPDKDVDGLTVTNAGRLASGLPALAPCTPTGCMMLIRDAIGDLKGKTAVVIGRSNLMGKPMAQMLLAADCTVTIAHSRS
QDLPSIVRQADIVVAAVGRAEMVKADWVKPGAVVIDVGITRFPARDPEAAAAGKTRLVGDVAFDEVREVAGAITPVPGGV
GPMTIACLLANTLTAAKRLSGIA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=42.9530201342282, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI222418558, Length=345, Percent_Identity=39.1304347826087, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI94721354, Length=301, Percent_Identity=42.5249169435216, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI36796743, Length=257, Percent_Identity=27.6264591439689, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786741, Length=287, Percent_Identity=45.993031358885, Blast_Score=247, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17568735, Length=297, Percent_Identity=40.0673400673401, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=294, Percent_Identity=43.8775510204082, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=42.809364548495, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=47.4916387959866, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI17136818, Length=299, Percent_Identity=47.4916387959866, Blast_Score=254, Evalue=9e-68,
Organism=Drosophila melanogaster, GI62472483, Length=297, Percent_Identity=45.4545454545455, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI45551871, Length=297, Percent_Identity=45.4545454545455, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24645718, Length=297, Percent_Identity=45.4545454545455, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI17137370, Length=297, Percent_Identity=45.4545454545455, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 35723; Mature: 35592

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGSREDLCHGARTRRGGEAVKSPGGRAPLRVSRPEARDGQRRRTPMAQAKLIDGKAFA
CCCCCHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCCCCHHHCCCHHHHEEECCHHHH
ADLRAKIAEEVAALKAEHGVTPGLAVVLVGEDPASQVYVRNKGEQTTAAGMYSETHRLPE
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
TTTQDELLAVVAKLNADPKIHGVLVQFPVPPHISQMDVVAALSPDKDVDGLTVTNAGRLA
CCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHEEEECCCCCCCCCEEEECCCHHH
SGLPALAPCTPTGCMMLIRDAIGDLKGKTAVVIGRSNLMGKPMAQMLLAADCTVTIAHSR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCC
SQDLPSIVRQADIVVAAVGRAEMVKADWVKPGAVVIDVGITRFPARDPEAAAAGKTRLVG
CCCHHHHHHHHCEEEEECCCHHHHHHHCCCCCEEEEEECCEECCCCCCCHHHCCCCCEEH
DVAFDEVREVAGAITPVPGGVGPMTIACLLANTLTAAKRLSGIA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGGSREDLCHGARTRRGGEAVKSPGGRAPLRVSRPEARDGQRRRTPMAQAKLIDGKAFA
CCCCHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCCCCHHHCCCHHHHEEECCHHHH
ADLRAKIAEEVAALKAEHGVTPGLAVVLVGEDPASQVYVRNKGEQTTAAGMYSETHRLPE
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
TTTQDELLAVVAKLNADPKIHGVLVQFPVPPHISQMDVVAALSPDKDVDGLTVTNAGRLA
CCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHEEEECCCCCCCCCEEEECCCHHH
SGLPALAPCTPTGCMMLIRDAIGDLKGKTAVVIGRSNLMGKPMAQMLLAADCTVTIAHSR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCC
SQDLPSIVRQADIVVAAVGRAEMVKADWVKPGAVVIDVGITRFPARDPEAAAAGKTRLVG
CCCHHHHHHHHCEEEEECCCHHHHHHHCCCCCEEEEEECCEECCCCCCCHHHCCCCCEEH
DVAFDEVREVAGAITPVPGGVGPMTIACLLANTLTAAKRLSGIA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA