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The map label for this gene is prfC [H]

Identifier: 16125322

GI number: 16125322

Start: 1201710

End: 1203314

Strand: Direct

Name: prfC [H]

Synonym: CC_1070

Alternate gene names: 16125322

Gene position: 1201710-1203314 (Clockwise)

Preceding gene: 16125320

Following gene: 16125326

Centisome position: 29.92

GC content: 67.17

Gene sequence:

>1605_bases
ATGAGCACTGCACCCGCCGCTGAAGCCGCCCGTCGGCGCACCTTCGCCATCATCAGCCACCCCGACGCCGGCAAGACGAC
CCTGACGGAAAACATCCTGCTGGCCGGCGGGGCGATCCGGGCCGCCGGCGCCGTGCGGGCGCGCGGCGAGAACCGTCGCA
CCCGGTCGGACTGGATGAAGATCGAGCGCGAGCGCGGGATCTCGGTCAGCGCCTCGGTGATGACGTTCGACCACGACGGC
AAGATGTTCAACCTCTTGGACACGCCGGGCCACGAGGATTTCTCGGAGGACACCTACCGCACCCTGACGGCCGCCGACGC
GGCGATCATGGTGCTGGACGCCGCCAAGGGCATCGAGCCCCAGACCCTGAAGCTGTTCGAGGTCTGCCGCCTGCGCGACA
TCCCGATCATCACCTTCATCAACAAGATGGACCGCGAGGCCCAGGACCCGATCGAGCTCTTGGACGAGATCGCGTCCAAG
CTGGCCCTGGATCCGGCCCCTCTGTACTGGCCGGCGGGGTCGGGCGGCCGCTTCAAGGGCATGCTGGACATGCGCGAAGG
GATGTTCATTCCCTACGCCAAGCGGACCGGCGGCGCAGCCGATGACGAGGGCCACCCCGAGCCTGTCGCCTTCGCCGGCA
ACTCGATCAACCAGTTCCTGGAAGACGCCGAGAAGGCCGAGCTGGAAGAGCAGGCCGAGCTGGTCGGCGGCGGTTATCCG
ACGTTCGAGGTCCAGTCGTTCCTGGAAGGCCACATGACGCCGGTGTTCTTCGGCTCGGCCCTGCGCCATTTCGGCGTCAA
GCAGCTCTTGGCCGGCATCGGCGCCTACGCCCCGTCGCCCAAGGCCATGAAGGCCGAAAAGAACGGTGAGCCGACCAGCG
TCGAGCCCGGCGACAAGGAGGTCTCGGGCTTCGTCTTCAAGGTCCAGGCGAACATGGACCCCAATCACCGCGACCGCATC
GCCATGCTGCGCCTGACCAGCGGCCGCTTCACGCGCGGCATGAAGCTGAAGGCCCAGAACACCGGCAAGGCCATGAGCGT
CAACGCGCCGATCATGTTCTTCGCCTCCGACCGCGAGCTGGCGGAAGACGCCTTCGCCGGCGACGTGATCGGCATTCCCA
ACCACGGCGTGCTGCGGGTGGGCGACAGCCTGTCCGAGAGCGGGACGCTGCGCTTCGCCGGCCTGCCCAACTTCGCGCCG
GAAATCCTGCGCCGCGTGCGCGTGAAGGATCCGCTGAAGGCCAAGCACCTGAAGAAGGCGCTGGAGGGCCTGGCCGAGGA
AGGCGTCACCCAGCTGTTCCGCCCGATGATCGGCAGCGACTTCATCGTCGGCGCCGTGGGCCAACTGCAGTTCGAGGTCA
TGGCCGACCGGCTGTCCAATGAGTACCAGCTGGACGTGATCTTCGAGAACAGCCCCTTCGCCGAGGCGCGCTGGCTGGTG
GGCGAACGCGCCGCCGTCGAGGACTTCGTCGACAAGCACAAGTCGGCCATGGGCCAGGACATCGACGAAGCGCCGGTGTT
CCTGGGCAAGAGCGCCTGGGAGATCGGCTACGTCGCCGACAAGTACCCGAAGGTGCGGTTCGAGCGGACCAAGGAACGGG
CGTAG

Upstream 100 bases:

>100_bases
GCCTGGCGCCAGCAGTTCGATGAGATTGTCGCGCGCCGCAAACCGGCCGCGTCGTCCGGCGCAGGTGACAAGGACGCTTC
GGACGGGTAGGTCCCCGCCC

Downstream 100 bases:

>100_bases
GGGCGTCAGGCGGCGGGCGCCGCCCCGCTCCGCTGGAGCGAACAGAGCCTACAGCGCCACGTAACCGTCGAGCTGCGCCA
ACTGAACGTGATCCGCCTCC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSTAPAAEAARRRTFAIISHPDAGKTTLTENILLAGGAIRAAGAVRARGENRRTRSDWMKIERERGISVSASVMTFDHDG
KMFNLLDTPGHEDFSEDTYRTLTAADAAIMVLDAAKGIEPQTLKLFEVCRLRDIPIITFINKMDREAQDPIELLDEIASK
LALDPAPLYWPAGSGGRFKGMLDMREGMFIPYAKRTGGAADDEGHPEPVAFAGNSINQFLEDAEKAELEEQAELVGGGYP
TFEVQSFLEGHMTPVFFGSALRHFGVKQLLAGIGAYAPSPKAMKAEKNGEPTSVEPGDKEVSGFVFKVQANMDPNHRDRI
AMLRLTSGRFTRGMKLKAQNTGKAMSVNAPIMFFASDRELAEDAFAGDVIGIPNHGVLRVGDSLSESGTLRFAGLPNFAP
EILRRVRVKDPLKAKHLKKALEGLAEEGVTQLFRPMIGSDFIVGAVGQLQFEVMADRLSNEYQLDVIFENSPFAEARWLV
GERAAVEDFVDKHKSAMGQDIDEAPVFLGKSAWEIGYVADKYPKVRFERTKERA

Sequences:

>Translated_534_residues
MSTAPAAEAARRRTFAIISHPDAGKTTLTENILLAGGAIRAAGAVRARGENRRTRSDWMKIERERGISVSASVMTFDHDG
KMFNLLDTPGHEDFSEDTYRTLTAADAAIMVLDAAKGIEPQTLKLFEVCRLRDIPIITFINKMDREAQDPIELLDEIASK
LALDPAPLYWPAGSGGRFKGMLDMREGMFIPYAKRTGGAADDEGHPEPVAFAGNSINQFLEDAEKAELEEQAELVGGGYP
TFEVQSFLEGHMTPVFFGSALRHFGVKQLLAGIGAYAPSPKAMKAEKNGEPTSVEPGDKEVSGFVFKVQANMDPNHRDRI
AMLRLTSGRFTRGMKLKAQNTGKAMSVNAPIMFFASDRELAEDAFAGDVIGIPNHGVLRVGDSLSESGTLRFAGLPNFAP
EILRRVRVKDPLKAKHLKKALEGLAEEGVTQLFRPMIGSDFIVGAVGQLQFEVMADRLSNEYQLDVIFENSPFAEARWLV
GERAAVEDFVDKHKSAMGQDIDEAPVFLGKSAWEIGYVADKYPKVRFERTKERA
>Mature_533_residues
STAPAAEAARRRTFAIISHPDAGKTTLTENILLAGGAIRAAGAVRARGENRRTRSDWMKIERERGISVSASVMTFDHDGK
MFNLLDTPGHEDFSEDTYRTLTAADAAIMVLDAAKGIEPQTLKLFEVCRLRDIPIITFINKMDREAQDPIELLDEIASKL
ALDPAPLYWPAGSGGRFKGMLDMREGMFIPYAKRTGGAADDEGHPEPVAFAGNSINQFLEDAEKAELEEQAELVGGGYPT
FEVQSFLEGHMTPVFFGSALRHFGVKQLLAGIGAYAPSPKAMKAEKNGEPTSVEPGDKEVSGFVFKVQANMDPNHRDRIA
MLRLTSGRFTRGMKLKAQNTGKAMSVNAPIMFFASDRELAEDAFAGDVIGIPNHGVLRVGDSLSESGTLRFAGLPNFAPE
ILRRVRVKDPLKAKHLKKALEGLAEEGVTQLFRPMIGSDFIVGAVGQLQFEVMADRLSNEYQLDVIFENSPFAEARWLVG
ERAAVEDFVDKHKSAMGQDIDEAPVFLGKSAWEIGYVADKYPKVRFERTKERA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=26.8041237113402, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI19923640, Length=508, Percent_Identity=27.9527559055118, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI25306287, Length=480, Percent_Identity=28.75, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI25306283, Length=380, Percent_Identity=30, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI157426893, Length=149, Percent_Identity=35.5704697986577, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=143, Percent_Identity=32.8671328671329, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=32.2368421052632, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790835, Length=540, Percent_Identity=44.8148148148148, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789738, Length=490, Percent_Identity=26.9387755102041, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=37.5796178343949, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=32.4137931034483, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=28.099173553719, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=183, Percent_Identity=36.0655737704918, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=172, Percent_Identity=31.9767441860465, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=31.8518518518519, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=31.8518518518519, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=28.0082987551867, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=527, Percent_Identity=24.4781783681214, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=29.9363057324841, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=34.2281879194631, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=34.2281879194631, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24582462, Length=518, Percent_Identity=27.6061776061776, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI221458488, Length=503, Percent_Identity=25.6461232604374, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.9622641509434, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24656849, Length=154, Percent_Identity=27.9220779220779, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574573, Length=155, Percent_Identity=30.9677419354839, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58614; Mature: 58483

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAPAAEAARRRTFAIISHPDAGKTTLTENILLAGGAIRAAGAVRARGENRRTRSDWMK
CCCCCHHHHHHCCEEEEEECCCCCCCCHHCCEEEECCCHHHCCHHHCCCCCCCCHHHHHH
IERERGISVSASVMTFDHDGKMFNLLDTPGHEDFSEDTYRTLTAADAAIMVLDAAKGIEP
HHHHCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
QTLKLFEVCRLRDIPIITFINKMDREAQDPIELLDEIASKLALDPAPLYWPAGSGGRFKG
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEE
MLDMREGMFIPYAKRTGGAADDEGHPEPVAFAGNSINQFLEDAEKAELEEQAELVGGGYP
EEEHHCCCEEEEHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TFEVQSFLEGHMTPVFFGSALRHFGVKQLLAGIGAYAPSPKAMKAEKNGEPTSVEPGDKE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
VSGFVFKVQANMDPNHRDRIAMLRLTSGRFTRGMKLKAQNTGKAMSVNAPIMFFASDREL
CCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEECCCEEEEECCHHH
AEDAFAGDVIGIPNHGVLRVGDSLSESGTLRFAGLPNFAPEILRRVRVKDPLKAKHLKKA
HHHHHCCCEEECCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHH
LEGLAEEGVTQLFRPMIGSDFIVGAVGQLQFEVMADRLSNEYQLDVIFENSPFAEARWLV
HHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
GERAAVEDFVDKHKSAMGQDIDEAPVFLGKSAWEIGYVADKYPKVRFERTKERA
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEECCHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
STAPAAEAARRRTFAIISHPDAGKTTLTENILLAGGAIRAAGAVRARGENRRTRSDWMK
CCCCHHHHHHCCEEEEEECCCCCCCCHHCCEEEECCCHHHCCHHHCCCCCCCCHHHHHH
IERERGISVSASVMTFDHDGKMFNLLDTPGHEDFSEDTYRTLTAADAAIMVLDAAKGIEP
HHHHCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
QTLKLFEVCRLRDIPIITFINKMDREAQDPIELLDEIASKLALDPAPLYWPAGSGGRFKG
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEE
MLDMREGMFIPYAKRTGGAADDEGHPEPVAFAGNSINQFLEDAEKAELEEQAELVGGGYP
EEEHHCCCEEEEHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TFEVQSFLEGHMTPVFFGSALRHFGVKQLLAGIGAYAPSPKAMKAEKNGEPTSVEPGDKE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
VSGFVFKVQANMDPNHRDRIAMLRLTSGRFTRGMKLKAQNTGKAMSVNAPIMFFASDREL
CCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEECCCEEEEECCHHH
AEDAFAGDVIGIPNHGVLRVGDSLSESGTLRFAGLPNFAPEILRRVRVKDPLKAKHLKKA
HHHHHCCCEEECCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHH
LEGLAEEGVTQLFRPMIGSDFIVGAVGQLQFEVMADRLSNEYQLDVIFENSPFAEARWLV
HHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
GERAAVEDFVDKHKSAMGQDIDEAPVFLGKSAWEIGYVADKYPKVRFERTKERA
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEECCHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA