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The map label for this gene is 16124467

Identifier: 16124467

GI number: 16124467

Start: 228693

End: 229571

Strand: Direct

Name: 16124467

Synonym: CC_0212

Alternate gene names: NA

Gene position: 228693-229571 (Clockwise)

Preceding gene: 16124466

Following gene: 16124468

Centisome position: 5.69

GC content: 67.01

Gene sequence:

>879_bases
ATGGCCCGCACGCTGAGCGTCGCCGCGATCCAGACCTCGTACGGCATGGATCTGCAGGCCAACATCAAGAAGACCGAAGG
CTTCATCCGCGAGGCTGCGTCGAAGGGCGCGCAGGTCATCCTGCCCTCGGAGCTGTTCCAGGGACCCTACTTCTGCGTCG
CCCAGGAAGAGCGCTGGTTTGCGCAGGCCCATCCGTGGCGCGAACACCCGGTGGTCAAGGCCATCGCCCCCCTGGCCGGC
GAGCTGGGCGTGGTGATCCCGATCTCGATCTTCGAGCGCGAAGGCCCGCACTACTTCAACAGCCTGGTGATGGCTGACGC
CGACGGCTCACTGATGGGCGTCTATCGCAAGAGCCATATCCCCGACGGTCCGGGCTATATGGAGAAGTACTACTTCCGGC
CCGGCGACACCGGCTTCAAGGTCTGGGACACCCGCTTTGGCCGGATCGGCGTCGGCATCTGCTGGGACCAATGGTACCCC
GAATGCGCCCGCGCCATGGCCCTGATGGGCGCCGAGGCCCTGTTCTATCCGACCGCCATCGGCAGCGAGCCGCATGACGC
CAGCCTCGACACCGCCCTGCCGTGGCGGCGGGCCATGCAGGGCCACGCGGTCAGCAATGTCATCCCGGTGATCGGCGCCA
ACCGCATCGGCTTCGAGCCGTGGGACGGCTATCCGAACGGCGGCCAGACCTTCTATGGCTCGTCCTTTGTGGCCGACCAT
CGCGGCGATCTGGTCAGCGAGCTGGGCCGCGCCGATGAGGGCCTGGTCAGCGCGACCTTCGATCTCGACTTCCTGACCAC
GCACCGCGCGGCCTGGGGCTTCTTCCGTGATCGCCGGCCGGAGCTCTATACGGCCCTGTCGAGCGGTCGACCCGCATGA

Upstream 100 bases:

>100_bases
TGTTCCCCGAGCGTCAGGTCATCGGCCTGCCGTCCACCGCCATCCTGACGGGCGGCGGTTCGTTCCACTGCATCAGCCAA
CAAGAGCCGGAGACCGCCTG

Downstream 100 bases:

>100_bases
TCGAAACAGACCGCCTGGTATTGCGCCCGGCGGTCGACGCCGATCGGGACGCGATCGCCGACCTGAACGCCCGGCCCGAA
GTGGGCGCCTGGCTGGGCGG

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWFAQAHPWREHPVVKAIAPLAG
ELGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHIPDGPGYMEKYYFRPGDTGFKVWDTRFGRIGVGICWDQWYP
ECARAMALMGAEALFYPTAIGSEPHDASLDTALPWRRAMQGHAVSNVIPVIGANRIGFEPWDGYPNGGQTFYGSSFVADH
RGDLVSELGRADEGLVSATFDLDFLTTHRAAWGFFRDRRPELYTALSSGRPA

Sequences:

>Translated_292_residues
MARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWFAQAHPWREHPVVKAIAPLAG
ELGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHIPDGPGYMEKYYFRPGDTGFKVWDTRFGRIGVGICWDQWYP
ECARAMALMGAEALFYPTAIGSEPHDASLDTALPWRRAMQGHAVSNVIPVIGANRIGFEPWDGYPNGGQTFYGSSFVADH
RGDLVSELGRADEGLVSATFDLDFLTTHRAAWGFFRDRRPELYTALSSGRPA
>Mature_291_residues
ARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWFAQAHPWREHPVVKAIAPLAGE
LGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHIPDGPGYMEKYYFRPGDTGFKVWDTRFGRIGVGICWDQWYPE
CARAMALMGAEALFYPTAIGSEPHDASLDTALPWRRAMQGHAVSNVIPVIGANRIGFEPWDGYPNGGQTFYGSSFVADHR
GDLVSELGRADEGLVSATFDLDFLTTHRAAWGFFRDRRPELYTALSSGRPA

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=275, Percent_Identity=28.3636363636364, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI9910460, Length=291, Percent_Identity=27.1477663230241, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI5031947, Length=291, Percent_Identity=28.5223367697594, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI297632348, Length=291, Percent_Identity=28.5223367697594, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI297632350, Length=291, Percent_Identity=28.5223367697594, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI297632346, Length=187, Percent_Identity=29.9465240641711, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17533173, Length=305, Percent_Identity=30.1639344262295, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17556280, Length=290, Percent_Identity=25.5172413793103, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=300, Percent_Identity=26.6666666666667, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322335, Length=310, Percent_Identity=24.1935483870968, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358471, Length=305, Percent_Identity=30.8196721311475, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32334; Mature: 32203

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWF
CCCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCEEEECHHHHCCCEEEEECCCHHH
AQAHPWREHPVVKAIAPLAGELGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHI
HHCCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCHHHHEEEEECCCCCEEEEHHHCCC
PDGPGYMEKYYFRPGDTGFKVWDTRFGRIGVGICWDQWYPECARAMALMGAEALFYPTAI
CCCCCHHHHEEECCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHHCCHHHEECCCC
GSEPHDASLDTALPWRRAMQGHAVSNVIPVIGANRIGFEPWDGYPNGGQTFYGSSFVADH
CCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCHH
RGDLVSELGRADEGLVSATFDLDFLTTHRAAWGFFRDRRPELYTALSSGRPA
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
ARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWF
CCEEEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCEEEECHHHHCCCEEEEECCCHHH
AQAHPWREHPVVKAIAPLAGELGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHI
HHCCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCHHHHEEEEECCCCCEEEEHHHCCC
PDGPGYMEKYYFRPGDTGFKVWDTRFGRIGVGICWDQWYPECARAMALMGAEALFYPTAI
CCCCCHHHHEEECCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHHCCHHHEECCCC
GSEPHDASLDTALPWRRAMQGHAVSNVIPVIGANRIGFEPWDGYPNGGQTFYGSSFVADH
CCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCHH
RGDLVSELGRADEGLVSATFDLDFLTTHRAAWGFFRDRRPELYTALSSGRPA
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]