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The map label for this gene is gyrB

Identifier: 16124415

GI number: 16124415

Start: 166829

End: 169246

Strand: Direct

Name: gyrB

Synonym: CC_0160

Alternate gene names: 16124415

Gene position: 166829-169246 (Clockwise)

Preceding gene: 16124414

Following gene: 16124416

Centisome position: 4.15

GC content: 66.83

Gene sequence:

>2418_bases
ATGACGACCGAGGAAGCCGCCGCCCAGTACGGCGCGGACTCGATCAAGGTGCTGAAGGGCCTCGACGCCGTCCGTAAGCG
TCCCGGCATGTATATCGGCGACACCGACGACGGTTCTGGCCTGCACCACATGGTCTATGAGGTGGTCGACAACGCCATCG
ACGAGGCCCTGGCCGGCCACGCCACCAAGGTCCAGGTGATCCTCAACGCCGACGGCTCGGTGACGGTCACCGATGACGGT
CGCGGTATCCCTGTCGACATGCACGAGGGCGAGGGCGTCTCGGCGGCCGAGGTCATCATGACCCAGCTGCACGCCGGCGG
TAAGTTCGACCAGAACAGCTACAAGGTCTCGGGCGGTCTGCACGGCGTGGGCGTCTCGGTCGTGAACGCCCTGTCGGATT
GGCTGGAGCTGCTGATCCACCGCAACGGCAAGGTCCACCAGATGCGCTTCGAGCGCGGCGACGCGGTCACCTCGCTGAAG
GTCACCGGCGACTCGCCCGTGCGGACCGAGGGCCCCAAGGCCGGCGAGACCCTGACCGGTACGGAAGTTACGTTCTTTCC
GTCGAAGGACACCTTCGCCTTCATCGAATTCGACCGGAAGACGCTGGAGCACCGCCTGCGCGAGCTGGCCTTCCTGAACT
CGGGCGTGACGATCTGGTTCAAGGACCATCGCGACGTCGAGCCGTGGGAAGAGAAGCTGTTCTACGAGGGCGGCATCGAG
GCCTTCGTGCGCCACCTCGACAAGGCCAAGACGCCGCTGCTGAAGGCCCCGATCGCCGTCAAGGGCGTCAAGGACAAGGT
CGAGATCGACCTGGCCCTGTGGTGGAACGACAGCTACCACGAGCAGATGCTGTGCTTCACCAACAACATCCCGCAGCGGG
ATGGCGGCACGCACCTGTCGGCCTTTCGCGCGGCCCTGACCCGGATCATCACCAGCTACGCCGAGAGCTCCGGCATCCTG
AAGAAGGAAAAGGTCAGCCTGGGCGGCGAAGACAGCCGCGAGGGCCTGACCTGCGTGCTGTCGGTCAAGGTCCCGGATCC
GAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTCTCGTCCGAAGTGCGCCCCGCCGTTGAGGGCCTGGTGTCGGAAGGTC
TCTCGACCTGGTTCGAGGAACATCCGAACGAGGCCAAGGCGATCGTGACCAAGATCGCCGAGGCCGCCGCCGCCCGCGAG
GCCGCCCGCAAGGCGCGAGAGCTGACCCGCCGCAAGAGCGCGCTCGACATCACCAGCCTGCCCGGCAAGCTCGCCGACTG
CTCGGAACGCGATCCGGCCAAGTCCGAGATCTTCATCGTCGAGGGCGACTCGGCGGGCGGCTCGGCCAAGCAGGCCCGCA
ACCGCGACAACCAGGCCGTTCTGCCCCTGCGCGGCAAGATCCTGAACGTCGAGCGGGCCCGCTTCGACAAGATGCTGTCG
TCCGACCAGATCGGCACGCTGATCACCGCCCTGGGCGCGGGGATCGGCCGCGACGACTTCAACCCGGACAAGGTGCGCTA
CCACAAGATCGTGCTGATGACCGACGCCGACGTCGACGGCGCCCACATCCGCACCCTGCTTCTGACCTTCTTCTACCGGC
AGATGCCGGAGCTGATCGAGCGCGGCTACATCTACATCGCCCAGCCGCCGCTCTACAAAGCCAGCAAGGGCAAGTCTTCG
CGCTACCTGAAGGACGACGCCGAGATGGACGCCTTCCTCGTCGATGAGGGCGTCGACGGGGCTGAGCTGGATCTGGCGTC
GGGTGAGCGGATGACGGGCCAGGACCTGCTGGCCTTGGTGCAGACCTGCCGCTCGGCCAAGGCCAATATTGACCGCCTGG
CCGCCCGCGCGCCGGCGACCGCGATCGAGCAGGCGGCCCTCTCTGGCCTTCTGGGCGAGTCGCCGAACGCCGCCGCGGCC
GCCACCCGCCTGGATCTCTACGCCGAGGAAGGCGATGGACCTTGGTCGGGCGAGCGCGGCGACACCGGCTTTGTGTTCAG
CCGCGTGCGTCGCGGTGTTTCGGAGCGGGTTGTTCTGGACGATGTGCTGCTGCACGCCGCCGACGCCCGTCGCCTGGCCG
AGCGCGCCGTCAAGCTGACCGAAATCTTCTCGGGTCGCGCCATCTTCCGCCGCAAGGACAAGTCGACGACCGTGCGCGGG
CCGCTCGACTTGGTCAACGCGGTGCTGGACGCCGGCCGCAAGGGCCTGACGATCCAGCGCTACAAGGGTCTGGGCGAGAT
GAACCCCGACCAGCTGTGGGAGACCACCCTGGACGCCGAAGCGCGCACCTTGCTGCAGGTCCGCGTCAACCACGCCGACG
ACGCCGACGACATGTTCAGCCGCCTTATGGGTGACTTGGTCGAGCCGCGCCGAGAGTTCATCCAGGAGAACGCCCTCGAC
GCCGAGGTCGACGTCTAA

Upstream 100 bases:

>100_bases
GGGCGCTAGGCGTTCGCGTGGGCGACGCCGGCCTGACTACTCTGGAAGACGAATGACCGAGAACACCGAAGACCAAGTTC
CCGACCTGTCGACCCCGGAA

Downstream 100 bases:

>100_bases
AGCTCATCCTTGCAGCTAAGTGTTGTTGCAAGTGTTTGTTCCATAAGCGCCGGGCGCCCAATCCAGGGGCGGCCCGGCGT
TTTCTTTTCCAGAAACGCCC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MTTEEAAAQYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATKVQVILNADGSVTVTDDG
RGIPVDMHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELLIHRNGKVHQMRFERGDAVTSLK
VTGDSPVRTEGPKAGETLTGTEVTFFPSKDTFAFIEFDRKTLEHRLRELAFLNSGVTIWFKDHRDVEPWEEKLFYEGGIE
AFVRHLDKAKTPLLKAPIAVKGVKDKVEIDLALWWNDSYHEQMLCFTNNIPQRDGGTHLSAFRAALTRIITSYAESSGIL
KKEKVSLGGEDSREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLVSEGLSTWFEEHPNEAKAIVTKIAEAAAARE
AARKARELTRRKSALDITSLPGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLRGKILNVERARFDKMLS
SDQIGTLITALGAGIGRDDFNPDKVRYHKIVLMTDADVDGAHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKASKGKSS
RYLKDDAEMDAFLVDEGVDGAELDLASGERMTGQDLLALVQTCRSAKANIDRLAARAPATAIEQAALSGLLGESPNAAAA
ATRLDLYAEEGDGPWSGERGDTGFVFSRVRRGVSERVVLDDVLLHAADARRLAERAVKLTEIFSGRAIFRRKDKSTTVRG
PLDLVNAVLDAGRKGLTIQRYKGLGEMNPDQLWETTLDAEARTLLQVRVNHADDADDMFSRLMGDLVEPRREFIQENALD
AEVDV

Sequences:

>Translated_805_residues
MTTEEAAAQYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATKVQVILNADGSVTVTDDG
RGIPVDMHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELLIHRNGKVHQMRFERGDAVTSLK
VTGDSPVRTEGPKAGETLTGTEVTFFPSKDTFAFIEFDRKTLEHRLRELAFLNSGVTIWFKDHRDVEPWEEKLFYEGGIE
AFVRHLDKAKTPLLKAPIAVKGVKDKVEIDLALWWNDSYHEQMLCFTNNIPQRDGGTHLSAFRAALTRIITSYAESSGIL
KKEKVSLGGEDSREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLVSEGLSTWFEEHPNEAKAIVTKIAEAAAARE
AARKARELTRRKSALDITSLPGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLRGKILNVERARFDKMLS
SDQIGTLITALGAGIGRDDFNPDKVRYHKIVLMTDADVDGAHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKASKGKSS
RYLKDDAEMDAFLVDEGVDGAELDLASGERMTGQDLLALVQTCRSAKANIDRLAARAPATAIEQAALSGLLGESPNAAAA
ATRLDLYAEEGDGPWSGERGDTGFVFSRVRRGVSERVVLDDVLLHAADARRLAERAVKLTEIFSGRAIFRRKDKSTTVRG
PLDLVNAVLDAGRKGLTIQRYKGLGEMNPDQLWETTLDAEARTLLQVRVNHADDADDMFSRLMGDLVEPRREFIQENALD
AEVDV
>Mature_804_residues
TTEEAAAQYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATKVQVILNADGSVTVTDDGR
GIPVDMHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELLIHRNGKVHQMRFERGDAVTSLKV
TGDSPVRTEGPKAGETLTGTEVTFFPSKDTFAFIEFDRKTLEHRLRELAFLNSGVTIWFKDHRDVEPWEEKLFYEGGIEA
FVRHLDKAKTPLLKAPIAVKGVKDKVEIDLALWWNDSYHEQMLCFTNNIPQRDGGTHLSAFRAALTRIITSYAESSGILK
KEKVSLGGEDSREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLVSEGLSTWFEEHPNEAKAIVTKIAEAAAAREA
ARKARELTRRKSALDITSLPGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLRGKILNVERARFDKMLSS
DQIGTLITALGAGIGRDDFNPDKVRYHKIVLMTDADVDGAHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKASKGKSSR
YLKDDAEMDAFLVDEGVDGAELDLASGERMTGQDLLALVQTCRSAKANIDRLAARAPATAIEQAALSGLLGESPNAAAAA
TRLDLYAEEGDGPWSGERGDTGFVFSRVRRGVSERVVLDDVLLHAADARRLAERAVKLTEIFSGRAIFRRKDKSTTVRGP
LDLVNAVLDAGRKGLTIQRYKGLGEMNPDQLWETTLDAEARTLLQVRVNHADDADDMFSRLMGDLVEPRREFIQENALDA
EVDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913408, Length=570, Percent_Identity=24.3859649122807, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI19913406, Length=557, Percent_Identity=24.5960502692998, Blast_Score=123, Evalue=7e-28,
Organism=Escherichia coli, GI48994957, Length=808, Percent_Identity=55.8168316831683, Blast_Score=836, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=568, Percent_Identity=40.4929577464789, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535065, Length=540, Percent_Identity=24.4444444444444, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=530, Percent_Identity=22.8301886792453, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=141, Percent_Identity=31.2056737588652, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=616, Percent_Identity=23.538961038961, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI17136538, Length=538, Percent_Identity=23.4200743494424, Blast_Score=117, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_CAUCN (B8GXQ0)

Other databases:

- EMBL:   CP001340
- EMBL:   U37793
- EMBL:   U00592
- ProteinModelPortal:   B8GXQ0
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00159
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK14939
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 88213; Mature: 88081

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEEAAAQYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGH
CCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
ATKVQVILNADGSVTVTDDGRGIPVDMHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGL
CEEEEEEEECCCCEEECCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSDWLELLIHRNGKVHQMRFERGDAVTSLKVTGDSPVRTEGPKAGETLTG
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEECC
TEVTFFPSKDTFAFIEFDRKTLEHRLRELAFLNSGVTIWFKDHRDVEPWEEKLFYEGGIE
CEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCHH
AFVRHLDKAKTPLLKAPIAVKGVKDKVEIDLALWWNDSYHEQMLCFTNNIPQRDGGTHLS
HHHHHHHHHCCCHHCCCEEEECCCCEEEEEEEEEECCCCHHEEHEEECCCCCCCCCCHHH
AFRAALTRIITSYAESSGILKKEKVSLGGEDSREGLTCVLSVKVPDPKFSSQTKDKLVSS
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
EVRPAVEGLVSEGLSTWFEEHPNEAKAIVTKIAEAAAAREAARKARELTRRKSALDITSL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
PGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLRGKILNVERARFDKMLS
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCEEEECCCEEECHHHHHHHHHHC
SDQIGTLITALGAGIGRDDFNPDKVRYHKIVLMTDADVDGAHIRTLLLTFFYRQMPELIE
CCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RGYIYIAQPPLYKASKGKSSRYLKDDAEMDAFLVDEGVDGAELDLASGERMTGQDLLALV
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHH
QTCRSAKANIDRLAARAPATAIEQAALSGLLGESPNAAAAATRLDLYAEEGDGPWSGERG
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCCCCCCC
DTGFVFSRVRRGVSERVVLDDVLLHAADARRLAERAVKLTEIFSGRAIFRRKDKSTTVRG
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCC
PLDLVNAVLDAGRKGLTIQRYKGLGEMNPDQLWETTLDAEARTLLQVRVNHADDADDMFS
HHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHEEEHEEECCCCCHHHHHH
RLMGDLVEPRREFIQENALDAEVDV
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTEEAAAQYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGH
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
ATKVQVILNADGSVTVTDDGRGIPVDMHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGL
CEEEEEEEECCCCEEECCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSDWLELLIHRNGKVHQMRFERGDAVTSLKVTGDSPVRTEGPKAGETLTG
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEECC
TEVTFFPSKDTFAFIEFDRKTLEHRLRELAFLNSGVTIWFKDHRDVEPWEEKLFYEGGIE
CEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCHH
AFVRHLDKAKTPLLKAPIAVKGVKDKVEIDLALWWNDSYHEQMLCFTNNIPQRDGGTHLS
HHHHHHHHHCCCHHCCCEEEECCCCEEEEEEEEEECCCCHHEEHEEECCCCCCCCCCHHH
AFRAALTRIITSYAESSGILKKEKVSLGGEDSREGLTCVLSVKVPDPKFSSQTKDKLVSS
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
EVRPAVEGLVSEGLSTWFEEHPNEAKAIVTKIAEAAAAREAARKARELTRRKSALDITSL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
PGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLRGKILNVERARFDKMLS
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCEEEECCCEEECHHHHHHHHHHC
SDQIGTLITALGAGIGRDDFNPDKVRYHKIVLMTDADVDGAHIRTLLLTFFYRQMPELIE
CCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RGYIYIAQPPLYKASKGKSSRYLKDDAEMDAFLVDEGVDGAELDLASGERMTGQDLLALV
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHH
QTCRSAKANIDRLAARAPATAIEQAALSGLLGESPNAAAAATRLDLYAEEGDGPWSGERG
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCCCCCCC
DTGFVFSRVRRGVSERVVLDDVLLHAADARRLAERAVKLTEIFSGRAIFRRKDKSTTVRG
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCC
PLDLVNAVLDAGRKGLTIQRYKGLGEMNPDQLWETTLDAEARTLLQVRVNHADDADDMFS
HHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHEEEHEEECCCCCHHHHHH
RLMGDLVEPRREFIQENALDAEVDV
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640