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The map label for this gene is recF

Identifier: 16124414

GI number: 16124414

Start: 165621

End: 166784

Strand: Direct

Name: recF

Synonym: CC_0159

Alternate gene names: 16124414

Gene position: 165621-166784 (Clockwise)

Preceding gene: 16124413

Following gene: 16124415

Centisome position: 4.12

GC content: 70.53

Gene sequence:

>1164_bases
ATGGCGTCGGCCGCACTGCTTTCCCTGACCCTGGCGGACTTTCGGTCCTACGAGCGCGCGCGCCTGGAGACCGGCGGTCG
CAGCGTCTACCTGTTCGGCGCGAACGGGGCGGGGAAAACCAATCTCCTCGAAGCCATCAGCCTGCTCAGCCCCGGCAAGG
GCTTGCGCGGGGTCAGTCTCGCCGAGGTCGGTCGCCGGCTGCCGGGCGAAGCGGTGGGGCGGGCCTGGGCCGTGGCCGCC
GAAGTTCAGTCCGGCGAGGATGCGCCGGTCAGGATCGGCACGGGCGTGGAGCAGGGCGGCGCGGCGCGTCGCACCGTGCG
GCTCGAGGGCGAGACCGTGCCGCCGGGCCGCCTCGCCGACCATGTGCGGCCGATCTGGCTGACCCCCGCCCAGGATCGAC
TTTTTCTCGAAGCCGCGTCCGAACGGCGCCGCTTTTTCGACCGGCTGGTCTTTGCGGGCGAACCGGCCCACGCCGCCAAC
GCCAACGGCTATGACAAGGCGCAACGCGAGCGTATGCGCCTGCTTGTCGACGCCGCCGAAACGGGCGCGCCCGCTGATGC
GGCCTGGCTGACCGCTCTGGAGGCTCGCCTGGCCGAGTTCGGCGCGCTTCTGGCCCAGGCGCGGGCGCGCACGCTGCTGG
CCCTGCAGGCCGAGATTGACGGACGGGGGGATCGCCCGTTCCCCCTCGCGCGCCTTGGCCTGACCGGAGAGTGGGAGCGC
ATGGCGCTGGAGGGCGCGCCGTTCGCCGAGATCGAGCTGAAACTGGCCCAGGCCCTGGCGTCCGCGCGGGCCCGCGACGG
CGCCGCCGGCAGGGCGTTGACGGGCCCGCATCGCGGCGATCTGGCGATCTTCCATGTCGAGAAGGACCGTCCGGCGGCGG
AATGCTCGACCGGCGAGCAAAAAGCCCTGATTTTGAACCTGGTTTTGGCCCAGGCGGCGCGACTTTCGCGTGCGGAATCC
GCGCCGAATCCTGTAATATTGCTCGACGAAGTCGCCGCGCATCTCGACCTTACCCGGCGAGCCGCTCTGGCTGACGAACT
CACGGCGCTCAAGCTCCAGGCCTTCCTGACCGGCACGGACGAGTCGCTGTTCGACCATCTCAAGGGTCGGGCGCTAGGCG
TTCGCGTGGGCGACGCCGGCCTGACTACTCTGGAAGACGAATGA

Upstream 100 bases:

>100_bases
GGAGGGCCATTCCGAGGCGGGGCCCGCTCCAGCGTCCTCATTGGAGCGGGCGTCTCGGCCCGCCTAGCCCCGGGGGCCGA
CACCGCTTAAGAACGCCCGC

Downstream 100 bases:

>100_bases
CCGAGAACACCGAAGACCAAGTTCCCGACCTGTCGACCCCGGAAATGACGACCGAGGAAGCCGCCGCCCAGTACGGCGCG
GACTCGATCAAGGTGCTGAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MASAALLSLTLADFRSYERARLETGGRSVYLFGANGAGKTNLLEAISLLSPGKGLRGVSLAEVGRRLPGEAVGRAWAVAA
EVQSGEDAPVRIGTGVEQGGAARRTVRLEGETVPPGRLADHVRPIWLTPAQDRLFLEAASERRRFFDRLVFAGEPAHAAN
ANGYDKAQRERMRLLVDAAETGAPADAAWLTALEARLAEFGALLAQARARTLLALQAEIDGRGDRPFPLARLGLTGEWER
MALEGAPFAEIELKLAQALASARARDGAAGRALTGPHRGDLAIFHVEKDRPAAECSTGEQKALILNLVLAQAARLSRAES
APNPVILLDEVAAHLDLTRRAALADELTALKLQAFLTGTDESLFDHLKGRALGVRVGDAGLTTLEDE

Sequences:

>Translated_387_residues
MASAALLSLTLADFRSYERARLETGGRSVYLFGANGAGKTNLLEAISLLSPGKGLRGVSLAEVGRRLPGEAVGRAWAVAA
EVQSGEDAPVRIGTGVEQGGAARRTVRLEGETVPPGRLADHVRPIWLTPAQDRLFLEAASERRRFFDRLVFAGEPAHAAN
ANGYDKAQRERMRLLVDAAETGAPADAAWLTALEARLAEFGALLAQARARTLLALQAEIDGRGDRPFPLARLGLTGEWER
MALEGAPFAEIELKLAQALASARARDGAAGRALTGPHRGDLAIFHVEKDRPAAECSTGEQKALILNLVLAQAARLSRAES
APNPVILLDEVAAHLDLTRRAALADELTALKLQAFLTGTDESLFDHLKGRALGVRVGDAGLTTLEDE
>Mature_386_residues
ASAALLSLTLADFRSYERARLETGGRSVYLFGANGAGKTNLLEAISLLSPGKGLRGVSLAEVGRRLPGEAVGRAWAVAAE
VQSGEDAPVRIGTGVEQGGAARRTVRLEGETVPPGRLADHVRPIWLTPAQDRLFLEAASERRRFFDRLVFAGEPAHAANA
NGYDKAQRERMRLLVDAAETGAPADAAWLTALEARLAEFGALLAQARARTLLALQAEIDGRGDRPFPLARLGLTGEWERM
ALEGAPFAEIELKLAQALASARARDGAAGRALTGPHRGDLAIFHVEKDRPAAECSTGEQKALILNLVLAQAARLSRAESA
PNPVILLDEVAAHLDLTRRAALADELTALKLQAFLTGTDESLFDHLKGRALGVRVGDAGLTTLEDE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=360, Percent_Identity=28.0555555555556, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CAUCN (B8GXP9)

Other databases:

- EMBL:   U37793
- EMBL:   CP001340
- EMBL:   U00593
- PIR:   B49347
- RefSeq:   YP_002515533.1
- ProteinModelPortal:   B8GXP9
- GeneID:   7332412
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_00158
- HOGENOM:   HBG645124
- OMA:   DDIMSHL
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- GO:   GO:0009295
- HAMAP:   MF_00365
- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406
- Gene3D:   G3DSA:3.40.1140.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41172; Mature: 41041

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAALLSLTLADFRSYERARLETGGRSVYLFGANGAGKTNLLEAISLLSPGKGLRGVSL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHH
AEVGRRLPGEAVGRAWAVAAEVQSGEDAPVRIGTGVEQGGAARRTVRLEGETVPPGRLAD
HHHHHCCCHHHHHHHHEEEEECCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCCHHHH
HVRPIWLTPAQDRLFLEAASERRRFFDRLVFAGEPAHAANANGYDKAQRERMRLLVDAAE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGAPADAAWLTALEARLAEFGALLAQARARTLLALQAEIDGRGDRPFPLARLGLTGEWER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEECCCCCHHH
MALEGAPFAEIELKLAQALASARARDGAAGRALTGPHRGDLAIFHVEKDRPAAECSTGEQ
HEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCH
KALILNLVLAQAARLSRAESAPNPVILLDEVAAHLDLTRRAALADELTALKLQAFLTGTD
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
ESLFDHLKGRALGVRVGDAGLTTLEDE
HHHHHHHCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ASAALLSLTLADFRSYERARLETGGRSVYLFGANGAGKTNLLEAISLLSPGKGLRGVSL
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHH
AEVGRRLPGEAVGRAWAVAAEVQSGEDAPVRIGTGVEQGGAARRTVRLEGETVPPGRLAD
HHHHHCCCHHHHHHHHEEEEECCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCCHHHH
HVRPIWLTPAQDRLFLEAASERRRFFDRLVFAGEPAHAANANGYDKAQRERMRLLVDAAE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGAPADAAWLTALEARLAEFGALLAQARARTLLALQAEIDGRGDRPFPLARLGLTGEWER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEECCCCCHHH
MALEGAPFAEIELKLAQALASARARDGAAGRALTGPHRGDLAIFHVEKDRPAAECSTGEQ
HEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCH
KALILNLVLAQAARLSRAESAPNPVILLDEVAAHLDLTRRAALADELTALKLQAFLTGTD
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
ESLFDHLKGRALGVRVGDAGLTTLEDE
HHHHHHHCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640