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The map label for this gene is pcm1 [H]

Identifier: 13488305

GI number: 13488305

Start: 306940

End: 307545

Strand: Direct

Name: pcm1 [H]

Synonym: mlr9350

Alternate gene names: 13488305

Gene position: 306940-307545 (Clockwise)

Preceding gene: 13488304

Following gene: 13488306

Centisome position: 87.22

GC content: 60.73

Gene sequence:

>606_bases
GTGCGCCACCTGCAACTGGAAACGATGGCGGGCGTTGCTGTGACAACAGGATATAAGGCTGAGTGGCAGCAAGCATGGTT
GAGGAAAGCACCGTCCGTCATGAAGCCAATGACCGAAGAGCACCTCGCGGTTCTACGCAGGCACATGGTCGAGATGATTG
CAATCCATACCGACCTTGCAAGCGAAGAACTTGGCAAGGCGGCGCTCGATGAGCGGGCGATGGCAGCGATGCGGCGGGTG
CCGCGGCATCGCTTCGTGCCAGCATCGGTTGTGCCTTACGCCTACCAGGACATGCCGCTGTGGATCGGCTTTGACAAAAC
CGTCTCGCAGCCCTTCATCGTTGCTCTCATGACCGATCTCCTTGCACCGCAACCGCACGAGGCGGTACTCGAGATCGGCA
CCGGCCTGGGCTACCAAACCGCAGTTCTCGCGAAGCTCGCCGGGCAAGTCTGTAGCGTCGAAATCGTCGAGGAATTCGCA
AGCAGTGCAGAGGCTCTCCTGCAGGGGGCTCGATTTATCCAATGTCGGCATTGGTGTCTGGGACGGGTCTCGCGGCTGGC
CCGAGCACGCCCCATTCGACAAGATCCTGGTCACGGCGGCGGCTGA

Upstream 100 bases:

>100_bases
GAGGGCTGAGAGCTCTTAGGGCCATAACTCCCACGGCTGCGGCACTCCCGATCGTCCGTGACCTCAACGGTCGTTCGTAC
GTCTGAGTTCGTTTCAGCGA

Downstream 100 bases:

>100_bases
GCAGACGCCGCCGGCTTTAGTGAAGCAACTCAAACCGGGGGGACGGCTGGTCCTGCCGTTGGGATCTGAAGAAGCACAGT
TTCTGACTGTCATCAACAAG

Product: hypothetical protein

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 1; Protein L-isoaspartyl methyltransferase 1; Protein-beta-aspartate methyltransferase 1; PIMT 1 [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRHLQLETMAGVAVTTGYKAEWQQAWLRKAPSVMKPMTEEHLAVLRRHMVEMIAIHTDLASEELGKAALDERAMAAMRRV
PRHRFVPASVVPYAYQDMPLWIGFDKTVSQPFIVALMTDLLAPQPHEAVLEIGTGLGYQTAVLAKLAGQVCSVEIVEEFA
SSAEALLQGARFIQCRHWCLGRVSRLARARPIRQDPGHGGG

Sequences:

>Translated_201_residues
MRHLQLETMAGVAVTTGYKAEWQQAWLRKAPSVMKPMTEEHLAVLRRHMVEMIAIHTDLASEELGKAALDERAMAAMRRV
PRHRFVPASVVPYAYQDMPLWIGFDKTVSQPFIVALMTDLLAPQPHEAVLEIGTGLGYQTAVLAKLAGQVCSVEIVEEFA
SSAEALLQGARFIQCRHWCLGRVSRLARARPIRQDPGHGGG
>Mature_201_residues
MRHLQLETMAGVAVTTGYKAEWQQAWLRKAPSVMKPMTEEHLAVLRRHMVEMIAIHTDLASEELGKAALDERAMAAMRRV
PRHRFVPASVVPYAYQDMPLWIGFDKTVSQPFIVALMTDLLAPQPHEAVLEIGTGLGYQTAVLAKLAGQVCSVEIVEEFA
SSAEALLQGARFIQCRHWCLGRVSRLARARPIRQDPGHGGG

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=88, Percent_Identity=48.8636363636364, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 22292; Mature: 22292

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLQLETMAGVAVTTGYKAEWQQAWLRKAPSVMKPMTEEHLAVLRRHMVEMIAIHTDLA
CCCCCHHHHHCCHHCCCCHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SEELGKAALDERAMAAMRRVPRHRFVPASVVPYAYQDMPLWIGFDKTVSQPFIVALMTDL
HHHHHHHHHHHHHHHHHHHCCHHCCCCCHHCCCHHCCCCEEEECCHHHCCHHHHHHHHHH
LAPQPHEAVLEIGTGLGYQTAVLAKLAGQVCSVEIVEEFASSAEALLQGARFIQCRHWCL
HCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVSRLARARPIRQDPGHGGG
HHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRHLQLETMAGVAVTTGYKAEWQQAWLRKAPSVMKPMTEEHLAVLRRHMVEMIAIHTDLA
CCCCCHHHHHCCHHCCCCHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SEELGKAALDERAMAAMRRVPRHRFVPASVVPYAYQDMPLWIGFDKTVSQPFIVALMTDL
HHHHHHHHHHHHHHHHHHHCCHHCCCCCHHCCCHHCCCCEEEECCHHHCCHHHHHHHHHH
LAPQPHEAVLEIGTGLGYQTAVLAKLAGQVCSVEIVEEFASSAEALLQGARFIQCRHWCL
HCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVSRLARARPIRQDPGHGGG
HHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA