Click here to switch to the map view.

The map label for this gene is frdA [H]

Identifier: 13488234

GI number: 13488234

Start: 172571

End: 174307

Strand: Direct

Name: frdA [H]

Synonym: mlr9192

Alternate gene names: 13488234

Gene position: 172571-174307 (Clockwise)

Preceding gene: 13488354

Following gene: 13488355

Centisome position: 49.04

GC content: 59.64

Gene sequence:

>1737_bases
ATGGACAATTTTGTGGAAGGGCTGTCGGAAGTCAGCTGCGATGTGCTGGTGATCGGCGGCGGCACTGCCGGACCGATGGC
GGCGCTCAAGGCGAAGCAGAAAAATCCTGCTCTGAAGGTCGTCCTGCTCGAAAAGGCCAATGTGAAGCGTTCGGGAGCTA
TCTGCATGGGCATGGACGGACTGAACAACGCCGTCGTTCCAGGATATGCGACGCCCGAGCAATACACCAAGGAGATCACG
ATCGCCAATGACGGTATCGTCGATCAGGCGCCGGTCTTTAAATATGCCTCGCGCTGCTATGATATTATCCAGGAACTTGA
CCGTTTCGGCATCCGTTTCCAGAAGGACGCCAATGGCGATTTCGACCTGAAGAAGGTCCACCATCTCGGAACCTATGTGC
TGCCGATGCCGAATGGCGATACCGTCAAGAAGGCGCTCTATCGGCAGATAAAGCGCGAACGGATATTGGTCTCAAACCGC
TTCATGGCAACGCGCCTGTTGACCGCCAAGGACGGCAGGATCGCGGGCGCCACTGCTGTGAACACGCGCACGGCTGAATT
CCTGGTACTGCGTGCCAAGACCGTGATCCTGTGCATGGGCGCGGCGGGCCGGCTCGGATTGCCGCATTCCGGCTATCTCT
TCGGAACCTATGAGAATCCGACCAATTCCGGTGACGGTTATGCCATGGCCTATCATGCGGGCGCCGCGCTCGCGAATCTC
GAATGCTATCAGATCAATCCGCTGATCAAGGACTACAACGGCTCGGCTTGCGCTTACGTTGCCGGTCCGTTCGGAGCCTA
TACCGCCAATAGTGAGGGCAACCGCTTCATCGAAAGCGACTACTGGTCGGGACAGATGATGCAGGAATTCTACAACGAAC
TACAGTCCGGCAAGGGGCCGGTGTTCCTGAAGCTCAACCACCTTCATGCCGACACGGTCAGCGAGATCGAAGAGATCCTG
CACAAGGTCGAGCGCCCGTCGCGCGGCAGGTTCCATCAGGCGCGGGGCACCGATTACCGCGAAAAGATGATCGAGATGCA
CATCTCCGAAATCGGCTTCTGCTCGGGTCACAGCGCGTCGGGCGTATTCGTCGATGAATTTGCCCGCACCACGGTGCCGG
GCCTCTACGCTGCGGGCGACATGGCGAGCGTGCCACACAACTATATGCTCGGCGCCTTCACCAATGGAGCCGTCGCCGGC
GAGCACGCTGCCGAAGTCGCGCTGGAAACCAGCCTCGCGGATTTCGACAGCGAGTTCGTCCGGGCGGAGCGCGAGCGCGT
GTTGGCACCGACGCGGCGTGAAGACGGCATCCCGCCCAACCAACTGGAATACAAGACCAGGCGGCTGGTCAACGACTATC
TGCAGCCGCCCAAGGTGACGGCCAGGATGCAGATCGGCCAGGCGCGGCTCGCCGAGGTCAGGGATGATCTCGAAACCGCC
CTCGTCGTACGCAACGCCCATGAACTGATGCGTTCGCTCGAAGTGTCGTCCATTCTCGACTGCGCCGACATGGCGGCGCA
TGCCTCGCTGTTCCGCACGGAGAGCCGCTGGGGGCTCTATCACAACCGCGTCGATTATCCCGAAAAGGATGACGACAACT
GGTTCTGCCATACGCTTCTTCGCAAGGTCGACGGCCGCTTGGTTTCTGAGAAGCGGCCAATCCAACCCTACATCGTTCCG
ATCGAGAACGAGGAGCGGACGGCCTATGACCGTCTGCGCGTTTCCAATCAAGCTTGA

Upstream 100 bases:

>100_bases
CTATGCCATACGTTTGGTTTTCTCCGACGGGCACGACCGGGGCATCTATCCCTGGAGCTATCTGCGCGAAATCGCTGATT
CCGAGGCTTGAGGAACGAGA

Downstream 100 bases:

>100_bases
CGAGGAAAACCATGCCGCTTGCGCTTTCACCCACGGCCGTTCCCGTCGTCGTGGACGAAACAAAATGCATCGCCGACAAA
GGCTGCACGGTCTGCGTCGA

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MDNFVEGLSEVSCDVLVIGGGTAGPMAALKAKQKNPALKVVLLEKANVKRSGAICMGMDGLNNAVVPGYATPEQYTKEIT
IANDGIVDQAPVFKYASRCYDIIQELDRFGIRFQKDANGDFDLKKVHHLGTYVLPMPNGDTVKKALYRQIKRERILVSNR
FMATRLLTAKDGRIAGATAVNTRTAEFLVLRAKTVILCMGAAGRLGLPHSGYLFGTYENPTNSGDGYAMAYHAGAALANL
ECYQINPLIKDYNGSACAYVAGPFGAYTANSEGNRFIESDYWSGQMMQEFYNELQSGKGPVFLKLNHLHADTVSEIEEIL
HKVERPSRGRFHQARGTDYREKMIEMHISEIGFCSGHSASGVFVDEFARTTVPGLYAAGDMASVPHNYMLGAFTNGAVAG
EHAAEVALETSLADFDSEFVRAERERVLAPTRREDGIPPNQLEYKTRRLVNDYLQPPKVTARMQIGQARLAEVRDDLETA
LVVRNAHELMRSLEVSSILDCADMAAHASLFRTESRWGLYHNRVDYPEKDDDNWFCHTLLRKVDGRLVSEKRPIQPYIVP
IENEERTAYDRLRVSNQA

Sequences:

>Translated_578_residues
MDNFVEGLSEVSCDVLVIGGGTAGPMAALKAKQKNPALKVVLLEKANVKRSGAICMGMDGLNNAVVPGYATPEQYTKEIT
IANDGIVDQAPVFKYASRCYDIIQELDRFGIRFQKDANGDFDLKKVHHLGTYVLPMPNGDTVKKALYRQIKRERILVSNR
FMATRLLTAKDGRIAGATAVNTRTAEFLVLRAKTVILCMGAAGRLGLPHSGYLFGTYENPTNSGDGYAMAYHAGAALANL
ECYQINPLIKDYNGSACAYVAGPFGAYTANSEGNRFIESDYWSGQMMQEFYNELQSGKGPVFLKLNHLHADTVSEIEEIL
HKVERPSRGRFHQARGTDYREKMIEMHISEIGFCSGHSASGVFVDEFARTTVPGLYAAGDMASVPHNYMLGAFTNGAVAG
EHAAEVALETSLADFDSEFVRAERERVLAPTRREDGIPPNQLEYKTRRLVNDYLQPPKVTARMQIGQARLAEVRDDLETA
LVVRNAHELMRSLEVSSILDCADMAAHASLFRTESRWGLYHNRVDYPEKDDDNWFCHTLLRKVDGRLVSEKRPIQPYIVP
IENEERTAYDRLRVSNQA
>Mature_578_residues
MDNFVEGLSEVSCDVLVIGGGTAGPMAALKAKQKNPALKVVLLEKANVKRSGAICMGMDGLNNAVVPGYATPEQYTKEIT
IANDGIVDQAPVFKYASRCYDIIQELDRFGIRFQKDANGDFDLKKVHHLGTYVLPMPNGDTVKKALYRQIKRERILVSNR
FMATRLLTAKDGRIAGATAVNTRTAEFLVLRAKTVILCMGAAGRLGLPHSGYLFGTYENPTNSGDGYAMAYHAGAALANL
ECYQINPLIKDYNGSACAYVAGPFGAYTANSEGNRFIESDYWSGQMMQEFYNELQSGKGPVFLKLNHLHADTVSEIEEIL
HKVERPSRGRFHQARGTDYREKMIEMHISEIGFCSGHSASGVFVDEFARTTVPGLYAAGDMASVPHNYMLGAFTNGAVAG
EHAAEVALETSLADFDSEFVRAERERVLAPTRREDGIPPNQLEYKTRRLVNDYLQPPKVTARMQIGQARLAEVRDDLETA
LVVRNAHELMRSLEVSSILDCADMAAHASLFRTESRWGLYHNRVDYPEKDDDNWFCHTLLRKVDGRLVSEKRPIQPYIVP
IENEERTAYDRLRVSNQA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=455, Percent_Identity=24.8351648351648, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790597, Length=614, Percent_Identity=22.6384364820847, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322701, Length=516, Percent_Identity=23.6434108527132, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322416, Length=550, Percent_Identity=23.8181818181818, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005884
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 64227; Mature: 64227

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFVEGLSEVSCDVLVIGGGTAGPMAALKAKQKNPALKVVLLEKANVKRSGAICMGMDG
CCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCC
LNNAVVPGYATPEQYTKEITIANDGIVDQAPVFKYASRCYDIIQELDRFGIRFQKDANGD
CCCCCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
FDLKKVHHLGTYVLPMPNGDTVKKALYRQIKRERILVSNRFMATRLLTAKDGRIAGATAV
CHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEHHEEEECCCCCEECEEEE
NTRTAEFLVLRAKTVILCMGAAGRLGLPHSGYLFGTYENPTNSGDGYAMAYHAGAALANL
CCCCCEEEEEEECEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHCCC
ECYQINPLIKDYNGSACAYVAGPFGAYTANSEGNRFIESDYWSGQMMQEFYNELQSGKGP
EEEEECCHHCCCCCCEEEEEECCCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHCCCCC
VFLKLNHLHADTVSEIEEILHKVERPSRGRFHQARGTDYREKMIEMHISEIGFCSGHSAS
EEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
GVFVDEFARTTVPGLYAAGDMASVPHNYMLGAFTNGAVAGEHAAEVALETSLADFDSEFV
CCHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
RAERERVLAPTRREDGIPPNQLEYKTRRLVNDYLQPPKVTARMQIGQARLAEVRDDLETA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHH
LVVRNAHELMRSLEVSSILDCADMAAHASLFRTESRWGLYHNRVDYPEKDDDNWFCHTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RKVDGRLVSEKRPIQPYIVPIENEERTAYDRLRVSNQA
HHHCCCEECCCCCCCCEEEEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDNFVEGLSEVSCDVLVIGGGTAGPMAALKAKQKNPALKVVLLEKANVKRSGAICMGMDG
CCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCC
LNNAVVPGYATPEQYTKEITIANDGIVDQAPVFKYASRCYDIIQELDRFGIRFQKDANGD
CCCCCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
FDLKKVHHLGTYVLPMPNGDTVKKALYRQIKRERILVSNRFMATRLLTAKDGRIAGATAV
CHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEHHEEEECCCCCEECEEEE
NTRTAEFLVLRAKTVILCMGAAGRLGLPHSGYLFGTYENPTNSGDGYAMAYHAGAALANL
CCCCCEEEEEEECEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHCCC
ECYQINPLIKDYNGSACAYVAGPFGAYTANSEGNRFIESDYWSGQMMQEFYNELQSGKGP
EEEEECCHHCCCCCCEEEEEECCCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHCCCCC
VFLKLNHLHADTVSEIEEILHKVERPSRGRFHQARGTDYREKMIEMHISEIGFCSGHSAS
EEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
GVFVDEFARTTVPGLYAAGDMASVPHNYMLGAFTNGAVAGEHAAEVALETSLADFDSEFV
CCHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
RAERERVLAPTRREDGIPPNQLEYKTRRLVNDYLQPPKVTARMQIGQARLAEVRDDLETA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHH
LVVRNAHELMRSLEVSSILDCADMAAHASLFRTESRWGLYHNRVDYPEKDDDNWFCHTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RKVDGRLVSEKRPIQPYIVPIENEERTAYDRLRVSNQA
HHHCCCEECCCCCCCCEEEEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]