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The map label for this gene is asnB [C]

Identifier: 13488222

GI number: 13488222

Start: 101956

End: 103518

Strand: Direct

Name: asnB [C]

Synonym: mlr9125

Alternate gene names: 13488222

Gene position: 101956-103518 (Clockwise)

Preceding gene: 13488221

Following gene: 13488223

Centisome position: 28.97

GC content: 55.15

Gene sequence:

>1563_bases
ATGACAGGTATTGCGCTTTCCCCAATCGGGGCGCTGTGGGGGATCGTCGGCGCCAAGACACACGGCACCGTGGAGATGGG
TGCGGCGCTGACACACCGCGGTTGCTACATGGCCATGGAGGAGGGACCAACATTCTCCTTTGGCGCCCGTCACTCCGCTG
AGATCTGGCCTATCTCTGTCGATGACGATATCGCCGTTGTCTATTCCGGTTTCCCATTTGTCGTTGATGAAGAGACACGG
ACGCCGCGTTATCTTTCAGCAGAAGATATCAGGACGGAATTCCTCAGATTCGGCGCCGATTTCTTCCGGGTTCTATGCGG
TCACTACGCAATCGCAGTCTACTGCTACACTCGTCACCGGCTGATCCTTGTTCGCGATGCGTTCGGCGTGCAGAGCCTTT
CGTGGGCGTCGTCGAATGAAAATTTTGCCTTTTCGACGGAGTATAAGGCACTGTTGTCACTGCCGTGGGTCGAACGAAAC
CTTGACCACGATGCCGCCCGCGGCTTCGTTGACGACGGCTGGGCGCCAGCGGGGCGAACCTTTCTGCGGCACATTTCCAT
GGTGCTGCCGGGATCAATCCTCATATGGCAGAATGGAAATCTTGAGACGATTCACCTGCGTCAAGATTTGTGCAAGACAC
GCCCACCGTTCAGACCGATTTCAGTTCGTCACGTAGAAATGACGGTTCGAGAGACGATCGGTTGGCAGGCGAATGATTTT
TCACCGAGATATGGGGTGATGCTTAGTAGCGGCGTCGACTCCGCAGTAATAGCGGCGACCCTGAGGCGAATTCGCAGGGA
TTCTGAGATTCACACATTCACTGTCGGCTATGGTTACGACGACCCGGAGATTATCGGTGCGAGCGCGACAGCGAGCGTCC
TTGGGACACAGCATCATGAAATCATAGTTCGGCCCGACGCCCTCACAGCGCTACTGACCGACACTATTGTCGCTGTGGAC
AATCCTGGCGGTTACGAAGAGTTCCCATGCCTTTATGCCCTGCATCAGGAAGCGCGACAATTCGTGGACATCATATTCTC
TGGCAATGCCGCTGACACGTTATTTGCAGGCATGCCGTATCATCGGGATTTGCTTTCGACCGATGACGCAATCTATCGGC
GGCTATGCAACGATCTAACTGTTCGCGACGAGCGCCTCGCTGCACAGGAACTCTTGGCTGGTAGACATGGTCTCAATTTT
AGGATGCCCTATGCGGAACGAATTCTCATAGACTTGGCGCTTGAAATCCCCGATTTTCAGAAACTCAGCCCTGAGAATGA
TAAGATTATCTTTCGTGAGGCGGCCCGTCAAATTCTGCCTGACGAAATCACCATGCGTCCCAAGCGGATTCAACACCTCC
TCTATGACGACAGCATGTGCTCGTGGCTGGCCGAGCGGATTGAGTGGATTTGCTCGAGCTCCCTTCTCCGGACGCATTCC
CTCGGAGAGCTCGAAGGGGCAGCAATCCTCCCGCCTCGTAATCTCGAACTTACGGAAGAGAGCTTCAAAGCCGCCTGGAA
CGTCGTCGCGCTTGCGACGTGGGCGAGTATCTACCTCGCCTAG

Upstream 100 bases:

>100_bases
TACCATGCCAGTGTTCCGTGTCGTATCAAAGTGAGCTGATGTACGTTGCCATCATTGTTTTCAGTGCGACGGCCTCCATG
TCACTCGTTGGAGCACACCT

Downstream 100 bases:

>100_bases
GACGGAGCGTCCGAATCTCTATCAACTAAATTCGGCAACGATCAATATCGACTGGAGACGAACCGTGACCGACTACTTCT
CCTACTTAGGTCTGCAACCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTGIALSPIGALWGIVGAKTHGTVEMGAALTHRGCYMAMEEGPTFSFGARHSAEIWPISVDDDIAVVYSGFPFVVDEETR
TPRYLSAEDIRTEFLRFGADFFRVLCGHYAIAVYCYTRHRLILVRDAFGVQSLSWASSNENFAFSTEYKALLSLPWVERN
LDHDAARGFVDDGWAPAGRTFLRHISMVLPGSILIWQNGNLETIHLRQDLCKTRPPFRPISVRHVEMTVRETIGWQANDF
SPRYGVMLSSGVDSAVIAATLRRIRRDSEIHTFTVGYGYDDPEIIGASATASVLGTQHHEIIVRPDALTALLTDTIVAVD
NPGGYEEFPCLYALHQEARQFVDIIFSGNAADTLFAGMPYHRDLLSTDDAIYRRLCNDLTVRDERLAAQELLAGRHGLNF
RMPYAERILIDLALEIPDFQKLSPENDKIIFREAARQILPDEITMRPKRIQHLLYDDSMCSWLAERIEWICSSSLLRTHS
LGELEGAAILPPRNLELTEESFKAAWNVVALATWASIYLA

Sequences:

>Translated_520_residues
MTGIALSPIGALWGIVGAKTHGTVEMGAALTHRGCYMAMEEGPTFSFGARHSAEIWPISVDDDIAVVYSGFPFVVDEETR
TPRYLSAEDIRTEFLRFGADFFRVLCGHYAIAVYCYTRHRLILVRDAFGVQSLSWASSNENFAFSTEYKALLSLPWVERN
LDHDAARGFVDDGWAPAGRTFLRHISMVLPGSILIWQNGNLETIHLRQDLCKTRPPFRPISVRHVEMTVRETIGWQANDF
SPRYGVMLSSGVDSAVIAATLRRIRRDSEIHTFTVGYGYDDPEIIGASATASVLGTQHHEIIVRPDALTALLTDTIVAVD
NPGGYEEFPCLYALHQEARQFVDIIFSGNAADTLFAGMPYHRDLLSTDDAIYRRLCNDLTVRDERLAAQELLAGRHGLNF
RMPYAERILIDLALEIPDFQKLSPENDKIIFREAARQILPDEITMRPKRIQHLLYDDSMCSWLAERIEWICSSSLLRTHS
LGELEGAAILPPRNLELTEESFKAAWNVVALATWASIYLA
>Mature_519_residues
TGIALSPIGALWGIVGAKTHGTVEMGAALTHRGCYMAMEEGPTFSFGARHSAEIWPISVDDDIAVVYSGFPFVVDEETRT
PRYLSAEDIRTEFLRFGADFFRVLCGHYAIAVYCYTRHRLILVRDAFGVQSLSWASSNENFAFSTEYKALLSLPWVERNL
DHDAARGFVDDGWAPAGRTFLRHISMVLPGSILIWQNGNLETIHLRQDLCKTRPPFRPISVRHVEMTVRETIGWQANDFS
PRYGVMLSSGVDSAVIAATLRRIRRDSEIHTFTVGYGYDDPEIIGASATASVLGTQHHEIIVRPDALTALLTDTIVAVDN
PGGYEEFPCLYALHQEARQFVDIIFSGNAADTLFAGMPYHRDLLSTDDAIYRRLCNDLTVRDERLAAQELLAGRHGLNFR
MPYAERILIDLALEIPDFQKLSPENDKIIFREAARQILPDEITMRPKRIQHLLYDDSMCSWLAERIEWICSSSLLRTHSL
GELEGAAILPPRNLELTEESFKAAWNVVALATWASIYLA

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=361, Percent_Identity=22.4376731301939, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI168229252, Length=361, Percent_Identity=22.4376731301939, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI168229250, Length=361, Percent_Identity=22.4376731301939, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI296010848, Length=361, Percent_Identity=22.4376731301939, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI296010852, Length=361, Percent_Identity=22.4376731301939, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI296010850, Length=361, Percent_Identity=22.4376731301939, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993933, Length=305, Percent_Identity=23.2786885245902, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560178, Length=272, Percent_Identity=27.5735294117647, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25147557, Length=305, Percent_Identity=23.2786885245902, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 58414; Mature: 58283

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIALSPIGALWGIVGAKTHGTVEMGAALTHRGCYMAMEEGPTFSFGARHSAEIWPISV
CCCCCCCHHHHHHHHHCCCCCCCEECCHHHHCCCEEEEECCCCCEECCCCCCCEEEEEEC
DDDIAVVYSGFPFVVDEETRTPRYLSAEDIRTEFLRFGADFFRVLCGHYAIAVYCYTRHR
CCCEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCE
LILVRDAFGVQSLSWASSNENFAFSTEYKALLSLPWVERNLDHDAARGFVDDGWAPAGRT
EEEEEECCCCCEEEECCCCCCEEEECCHHHHHCCCHHHCCCCHHHHCCCCCCCCCCHHHH
FLRHISMVLPGSILIWQNGNLETIHLRQDLCKTRPPFRPISVRHVEMTVRETIGWQANDF
HHHHHHHHCCCEEEEEECCCEEEEEEHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCC
SPRYGVMLSSGVDSAVIAATLRRIRRDSEIHTFTVGYGYDDPEIIGASATASVLGTQHHE
CCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEECCCHHHHHHCCCCCE
IIVRPDALTALLTDTIVAVDNPGGYEEFPCLYALHQEARQFVDIIFSGNAADTLFAGMPY
EEECCHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHEECCCC
HRDLLSTDDAIYRRLCNDLTVRDERLAAQELLAGRHGLNFRMPYAERILIDLALEIPDFQ
HHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCHHHHEEEEHEECCCCCC
KLSPENDKIIFREAARQILPDEITMRPKRIQHLLYDDSMCSWLAERIEWICSSSLLRTHS
CCCCCCCCEEHHHHHHHHCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
LGELEGAAILPPRNLELTEESFKAAWNVVALATWASIYLA
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGIALSPIGALWGIVGAKTHGTVEMGAALTHRGCYMAMEEGPTFSFGARHSAEIWPISV
CCCCCCHHHHHHHHHCCCCCCCEECCHHHHCCCEEEEECCCCCEECCCCCCCEEEEEEC
DDDIAVVYSGFPFVVDEETRTPRYLSAEDIRTEFLRFGADFFRVLCGHYAIAVYCYTRHR
CCCEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCE
LILVRDAFGVQSLSWASSNENFAFSTEYKALLSLPWVERNLDHDAARGFVDDGWAPAGRT
EEEEEECCCCCEEEECCCCCCEEEECCHHHHHCCCHHHCCCCHHHHCCCCCCCCCCHHHH
FLRHISMVLPGSILIWQNGNLETIHLRQDLCKTRPPFRPISVRHVEMTVRETIGWQANDF
HHHHHHHHCCCEEEEEECCCEEEEEEHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCC
SPRYGVMLSSGVDSAVIAATLRRIRRDSEIHTFTVGYGYDDPEIIGASATASVLGTQHHE
CCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEECCCHHHHHHCCCCCE
IIVRPDALTALLTDTIVAVDNPGGYEEFPCLYALHQEARQFVDIIFSGNAADTLFAGMPY
EEECCHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHEECCCC
HRDLLSTDDAIYRRLCNDLTVRDERLAAQELLAGRHGLNFRMPYAERILIDLALEIPDFQ
HHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCHHHHEEEEHEECCCCCC
KLSPENDKIIFREAARQILPDEITMRPKRIQHLLYDDSMCSWLAERIEWICSSSLLRTHS
CCCCCCCCEEHHHHHHHHCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
LGELEGAAILPPRNLELTEESFKAAWNVVALATWASIYLA
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]