Click here to switch to the map view.

The map label for this gene is yiaO [H]

Identifier: 13488172

GI number: 13488172

Start: 330527

End: 331744

Strand: Reverse

Name: yiaO [H]

Synonym: mll9374

Alternate gene names: 13488172

Gene position: 331744-330527 (Counterclockwise)

Preceding gene: 13488173

Following gene: 13488171

Centisome position: 94.27

GC content: 53.2

Gene sequence:

>1218_bases
GTGCAGTGTAGCGGCCGCGATCGAAGCAAGCGCGCAGGAGCGGTAATGGACGCAAGATCCGAAGTGGAAAAACAGATCAA
TGGCATGGTTGGAAAGCGGCTGCGCGAGGCCCGGAAGGCCAAGAGCCTTAGTCTCGAGGCCCTCGCTGGAAAGGTCGGCA
TGTCTGTGTCGCAGCTATCGAAGCTTGAAACGGGAAAGGCCCCGGCGTCGATCGCAGCTTTAGTGCGTTTCGGCCTGGAA
TTGGACCGATCAGCGCAATATTTTCTTCAGTCGGATTCTGAAATGCCGCGTTGTTTGGGCACTCTGGTACCGTCTTGGGA
TCCTGAGGGCAGAGCTATCGAGATGTTCTCGTCCCTAGTCAGCCAAAAAACAGGATACGATCTTGCGATTTCGGTATTTC
CCGGGGGCCAACTTGGTCCCGCTGAAACTCAAGTGCGAGCCCTCGTCAATGGAATTATTGACATTTTTGTCGAAAGCCTG
GGGTTTTTTCAGCACTATTCGGATCCCGTCAAACTAGCATCACTCCCGTTTTGCTTCGACGATGAAAAGCATCTGGAACG
ATTTTCGGAGAGCTCCTTGTTTGATCGAGAAGTCAGGCAGGCTCTCCGAAGCAATTCGGTCGAACTGCTAAATCCGAGCT
TGAAGTGGAAGCGGGGCCCTTCCCTAGTTTTGTTATCGCGGACCCCTATCTTATCTCCGGATGACCTAAGAGACTTGCCC
ATAAGGTGCCCTGAAAACGATACCATGTTCCGCTATCTCGAGATTATGGGCTGCCGACCTGTAGTTGTCCCGTGGAATGA
GGTGGGGGACGCGCTCCAGGACGGCAAATTTTCAGCGATGATTACCAATCTCAGTCATGTTGTGAGCATGCGTTTCACGC
GATTTGCTCGGTTCATGACAGCATTGGACTACCGGCCGCTCGACCTGACATTTGCAATGAACCTACAGCGTTATCAAATT
CTCGCTCCGCACATTCAAACCGGGCTGGAACAAGCTGCCATCGAGGCTGCAGAATTTTGTTCGAATTTGCTTGGCCACAC
GTGTGATCTTTTGCCTGCCCTATTGGAGGAGGACGGAGCGGTTTTGTGTAATGTCGGGGTAAAGGCGTGGCGCGCAAAGT
CCCGCCAGGTTTTTGAAGAGATGGAAAAGGAGGGCGCCTGGCGTAGTGGTCTGGTCAAGGAGATCAGCGCACTAGCCCAA
GTTGCAGGCGACACTTGA

Upstream 100 bases:

>100_bases
TTCTTGTGCATCTGCTACCATCTGTTTTTTCCGATCGGGACGTGTTAGGGGTGAACAAAGCGGCCCAGAGATTTCCCCTG
CCTTCCCCCACACGCGTTAG

Downstream 100 bases:

>100_bases
ATTTTCTTGACAAGAAAATTTGGAGGGCTAAGACAGGCTAGTAATTTTCGTACTGACAAAGGCCGCGATTACGATGGCGT
TTGCGCTACCTATTCCAATT

Product: hypothetical protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MQCSGRDRSKRAGAVMDARSEVEKQINGMVGKRLREARKAKSLSLEALAGKVGMSVSQLSKLETGKAPASIAALVRFGLE
LDRSAQYFLQSDSEMPRCLGTLVPSWDPEGRAIEMFSSLVSQKTGYDLAISVFPGGQLGPAETQVRALVNGIIDIFVESL
GFFQHYSDPVKLASLPFCFDDEKHLERFSESSLFDREVRQALRSNSVELLNPSLKWKRGPSLVLLSRTPILSPDDLRDLP
IRCPENDTMFRYLEIMGCRPVVVPWNEVGDALQDGKFSAMITNLSHVVSMRFTRFARFMTALDYRPLDLTFAMNLQRYQI
LAPHIQTGLEQAAIEAAEFCSNLLGHTCDLLPALLEEDGAVLCNVGVKAWRAKSRQVFEEMEKEGAWRSGLVKEISALAQ
VAGDT

Sequences:

>Translated_405_residues
MQCSGRDRSKRAGAVMDARSEVEKQINGMVGKRLREARKAKSLSLEALAGKVGMSVSQLSKLETGKAPASIAALVRFGLE
LDRSAQYFLQSDSEMPRCLGTLVPSWDPEGRAIEMFSSLVSQKTGYDLAISVFPGGQLGPAETQVRALVNGIIDIFVESL
GFFQHYSDPVKLASLPFCFDDEKHLERFSESSLFDREVRQALRSNSVELLNPSLKWKRGPSLVLLSRTPILSPDDLRDLP
IRCPENDTMFRYLEIMGCRPVVVPWNEVGDALQDGKFSAMITNLSHVVSMRFTRFARFMTALDYRPLDLTFAMNLQRYQI
LAPHIQTGLEQAAIEAAEFCSNLLGHTCDLLPALLEEDGAVLCNVGVKAWRAKSRQVFEEMEKEGAWRSGLVKEISALAQ
VAGDT
>Mature_405_residues
MQCSGRDRSKRAGAVMDARSEVEKQINGMVGKRLREARKAKSLSLEALAGKVGMSVSQLSKLETGKAPASIAALVRFGLE
LDRSAQYFLQSDSEMPRCLGTLVPSWDPEGRAIEMFSSLVSQKTGYDLAISVFPGGQLGPAETQVRALVNGIIDIFVESL
GFFQHYSDPVKLASLPFCFDDEKHLERFSESSLFDREVRQALRSNSVELLNPSLKWKRGPSLVLLSRTPILSPDDLRDLP
IRCPENDTMFRYLEIMGCRPVVVPWNEVGDALQDGKFSAMITNLSHVVSMRFTRFARFMTALDYRPLDLTFAMNLQRYQI
LAPHIQTGLEQAAIEAAEFCSNLLGHTCDLLPALLEEDGAVLCNVGVKAWRAKSRQVFEEMEKEGAWRSGLVKEISALAQ
VAGDT

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=265, Percent_Identity=23.7735849056604, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 44941; Mature: 44941

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCSGRDRSKRAGAVMDARSEVEKQINGMVGKRLREARKAKSLSLEALAGKVGMSVSQLS
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KLETGKAPASIAALVRFGLELDRSAQYFLQSDSEMPRCLGTLVPSWDPEGRAIEMFSSLV
HHHCCCCHHHHHHHHHHCCHHCCCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
SQKTGYDLAISVFPGGQLGPAETQVRALVNGIIDIFVESLGFFQHYSDPVKLASLPFCFD
HHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
DEKHLERFSESSLFDREVRQALRSNSVELLNPSLKWKRGPSLVLLSRTPILSPDDLRDLP
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCC
IRCPENDTMFRYLEIMGCRPVVVPWNEVGDALQDGKFSAMITNLSHVVSMRFTRFARFMT
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALDYRPLDLTFAMNLQRYQILAPHIQTGLEQAAIEAAEFCSNLLGHTCDLLPALLEEDGA
HHCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLCNVGVKAWRAKSRQVFEEMEKEGAWRSGLVKEISALAQVAGDT
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQCSGRDRSKRAGAVMDARSEVEKQINGMVGKRLREARKAKSLSLEALAGKVGMSVSQLS
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KLETGKAPASIAALVRFGLELDRSAQYFLQSDSEMPRCLGTLVPSWDPEGRAIEMFSSLV
HHHCCCCHHHHHHHHHHCCHHCCCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
SQKTGYDLAISVFPGGQLGPAETQVRALVNGIIDIFVESLGFFQHYSDPVKLASLPFCFD
HHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
DEKHLERFSESSLFDREVRQALRSNSVELLNPSLKWKRGPSLVLLSRTPILSPDDLRDLP
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCC
IRCPENDTMFRYLEIMGCRPVVVPWNEVGDALQDGKFSAMITNLSHVVSMRFTRFARFMT
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALDYRPLDLTFAMNLQRYQILAPHIQTGLEQAAIEAAEFCSNLLGHTCDLLPALLEEDGA
HHCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLCNVGVKAWRAKSRQVFEEMEKEGAWRSGLVKEISALAQVAGDT
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]