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The map label for this gene is ecaIM [H]

Identifier: 13488060

GI number: 13488060

Start: 55097

End: 56941

Strand: Reverse

Name: ecaIM [H]

Synonym: mll9056

Alternate gene names: 13488060

Gene position: 56941-55097 (Counterclockwise)

Preceding gene: 13488324

Following gene: 13488059

Centisome position: 16.18

GC content: 55.28

Gene sequence:

>1845_bases
ATGAGCCGTGCACGCATTGCGGCGGGAGCGGCCGACCAGGGCGGCAGGTGGCCCGGGCAATGGTTAACCGCTTATCAGCG
AAGTTCCTGCAAAGTATCATTCGCAGCAGTGGGGCCCGAGATGAAAAATCAGCTTTGGTTCGGAGATAATTTGACGATTC
TCCGGGAAGAAATTGCAGACGAAAGCGTGGATCTCGTCTATCTCGATCCGCCGTTTAACAGCCAAGTCAACTACAACGTC
CTCTTTCGAACTCCCGATGAAGATGCCGCCAGCGCTCAAGTAGAGGCATTCCGAGATACGTGGACTTGGGGGCCAGAGGC
CAAGTGGGCTTTCGATGAGATTATGCATACTGGCGGTGGTGTCACGCCGATTGTGCATGCTCTGCATGCAGCGCTCGGTG
ACAGCGATATGATGGCCTATTTGGTCATGATGGCGCAACGGTTGCACGAGTTACGACGCGTTCTAAGGCCAACAGGAAGC
CTTTATTTGCATTGCGATCCAACGGCGAGCCATTACCTCAAGATCATTCTCGACGCGATTTTCGGCCCGACGAATTTTCT
GAACGAGGTAATCTGGAAAAGAACTAGCGCACATAGTAGCGCACGACGTTACGGGCCAATCCATGACGTTTTGCTGTTCT
ATTCGCGTGGTGAACGGCATTGCTGGAATGCTCAGTATCAGCCTTACGACGCAGAATATTTGGACACTTTTTTTGATCAG
GAGGACGCTGACGGCCGGCGTTGGAAGCGCACTGATCTTACCGGTGCGGGAAAGAGGAATGGCGAAACTGGTAAACCTTG
GCGCGGCATCGACATTACGGCCAAGGGAAGGCATTGGTCGACGTCGCCTGACCAACTTGACGCCCTTGATCTTGCTGGGA
AGGTTCATTGGCCGAAGAAGGCGGGGGGTATGCCGCGACTCCGGCAGTATCCCGAGGATCTGCCTGGTGTCGCGCTTCAG
GACATTTGGACGGACATCAAACCGCTGCATAATCGAGCGGCGGAACGATTGCACTACCCCACTCAGAAACCTGTGTCGCT
GTTAGATAGAATACTCCGGTCTTCCGCCAATGTGGGCGAGGTGGTGCTGGATCCATTCTGCGGCTGCGGAACGACCATTG
CTGCTGCCCAGTTGGCCGGTCGTCAATGGATCGGTATTGATGTGGCGTATCATGCCATCCGTGTGATCGAAGACCGCTTG
GCCGATATGCCTGGTAAGATTGACTATGATCTTGGCGGCATCCCCCGCGACTTCGCCAGTGCTGCCCGTCTCGCCGAGCG
GGACAAATACCAATTTCAATGGTGGGCCAACTACCTGGTTGGCGTCCAAGCGCTAAAGGAAATTCGAAAAGGCCCGGACC
GTGGTATCGACGGTCAAATGTATTTCATGAACGGGCCGCGCGGCTGGGGGCGTGTGCTGACATCCGTTAAAGGCGGTCAG
CATATCGGCACCAAAGATGTGCGTGAGTTCAAAGCTGTGATCGATCGTGAACGCGCGGAAATGGGCTTATTCATCTGCCT
CCGAGAACCCACGCGCGACATGCGTTCGGAAGCCGCGTCCTTCGGCTTTGTCGATACTGCCCACGGTCACCTGCCCAGAT
TGCAGATCGTTTCAATCGAGCGCTGGTTCAATGGGGAGCGCCCAACTATCCCTTCGCTTGGGCATCTCTCACGGGAATTT
TTCCGCCAATCTTCCAAACCTGAAAAGAGCAAGAAACCGAGGCGTCCGGACCCAACCGCACCTGAGTTCCCATTCAGCTT
TATCGGCGACGGAAAGGACGCGGTTGTGCATTTCAACCCGACCGCAGTCCCCAAGGAACATGGGCTTTCTGAAGGGTTGC
TGTAG

Upstream 100 bases:

>100_bases
CGCTCGCAAGAAACGCTATCATTTTCATTGATTTCCCCCCGGAAAAATACTTGCGCAAATTCTGTACGGAAGTCCAGAGG
CTTGTTGAGTCCGCGTATCG

Downstream 100 bases:

>100_bases
ACAGTCGAGATCAAAATGGGTTTGGCCGGTCCTCGAGGTTTCAGCACGAACTTTCACACGGTCTTCCGTCCTTGTCGCGG
TTGAGCTTGCCGCCCCAGGG

Product: DNA methyltransferase

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSRARIAAGAADQGGRWPGQWLTAYQRSSCKVSFAAVGPEMKNQLWFGDNLTILREEIADESVDLVYLDPPFNSQVNYNV
LFRTPDEDAASAQVEAFRDTWTWGPEAKWAFDEIMHTGGGVTPIVHALHAALGDSDMMAYLVMMAQRLHELRRVLRPTGS
LYLHCDPTASHYLKIILDAIFGPTNFLNEVIWKRTSAHSSARRYGPIHDVLLFYSRGERHCWNAQYQPYDAEYLDTFFDQ
EDADGRRWKRTDLTGAGKRNGETGKPWRGIDITAKGRHWSTSPDQLDALDLAGKVHWPKKAGGMPRLRQYPEDLPGVALQ
DIWTDIKPLHNRAAERLHYPTQKPVSLLDRILRSSANVGEVVLDPFCGCGTTIAAAQLAGRQWIGIDVAYHAIRVIEDRL
ADMPGKIDYDLGGIPRDFASAARLAERDKYQFQWWANYLVGVQALKEIRKGPDRGIDGQMYFMNGPRGWGRVLTSVKGGQ
HIGTKDVREFKAVIDRERAEMGLFICLREPTRDMRSEAASFGFVDTAHGHLPRLQIVSIERWFNGERPTIPSLGHLSREF
FRQSSKPEKSKKPRRPDPTAPEFPFSFIGDGKDAVVHFNPTAVPKEHGLSEGLL

Sequences:

>Translated_614_residues
MSRARIAAGAADQGGRWPGQWLTAYQRSSCKVSFAAVGPEMKNQLWFGDNLTILREEIADESVDLVYLDPPFNSQVNYNV
LFRTPDEDAASAQVEAFRDTWTWGPEAKWAFDEIMHTGGGVTPIVHALHAALGDSDMMAYLVMMAQRLHELRRVLRPTGS
LYLHCDPTASHYLKIILDAIFGPTNFLNEVIWKRTSAHSSARRYGPIHDVLLFYSRGERHCWNAQYQPYDAEYLDTFFDQ
EDADGRRWKRTDLTGAGKRNGETGKPWRGIDITAKGRHWSTSPDQLDALDLAGKVHWPKKAGGMPRLRQYPEDLPGVALQ
DIWTDIKPLHNRAAERLHYPTQKPVSLLDRILRSSANVGEVVLDPFCGCGTTIAAAQLAGRQWIGIDVAYHAIRVIEDRL
ADMPGKIDYDLGGIPRDFASAARLAERDKYQFQWWANYLVGVQALKEIRKGPDRGIDGQMYFMNGPRGWGRVLTSVKGGQ
HIGTKDVREFKAVIDRERAEMGLFICLREPTRDMRSEAASFGFVDTAHGHLPRLQIVSIERWFNGERPTIPSLGHLSREF
FRQSSKPEKSKKPRRPDPTAPEFPFSFIGDGKDAVVHFNPTAVPKEHGLSEGLL
>Mature_613_residues
SRARIAAGAADQGGRWPGQWLTAYQRSSCKVSFAAVGPEMKNQLWFGDNLTILREEIADESVDLVYLDPPFNSQVNYNVL
FRTPDEDAASAQVEAFRDTWTWGPEAKWAFDEIMHTGGGVTPIVHALHAALGDSDMMAYLVMMAQRLHELRRVLRPTGSL
YLHCDPTASHYLKIILDAIFGPTNFLNEVIWKRTSAHSSARRYGPIHDVLLFYSRGERHCWNAQYQPYDAEYLDTFFDQE
DADGRRWKRTDLTGAGKRNGETGKPWRGIDITAKGRHWSTSPDQLDALDLAGKVHWPKKAGGMPRLRQYPEDLPGVALQD
IWTDIKPLHNRAAERLHYPTQKPVSLLDRILRSSANVGEVVLDPFCGCGTTIAAAQLAGRQWIGIDVAYHAIRVIEDRLA
DMPGKIDYDLGGIPRDFASAARLAERDKYQFQWWANYLVGVQALKEIRKGPDRGIDGQMYFMNGPRGWGRVLTSVKGGQH
IGTKDVREFKAVIDRERAEMGLFICLREPTRDMRSEAASFGFVDTAHGHLPRLQIVSIERWFNGERPTIPSLGHLSREFF
RQSSKPEKSKKPRRPDPTAPEFPFSFIGDGKDAVVHFNPTAVPKEHGLSEGLL

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 69231; Mature: 69100

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRARIAAGAADQGGRWPGQWLTAYQRSSCKVSFAAVGPEMKNQLWFGDNLTILREEIAD
CCCCCEECCCCCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHCCEEECCCHHHHHHHHCC
ESVDLVYLDPPFNSQVNYNVLFRTPDEDAASAQVEAFRDTWTWGPEAKWAFDEIMHTGGG
CCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
VTPIVHALHAALGDSDMMAYLVMMAQRLHELRRVLRPTGSLYLHCDPTASHYLKIILDAI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
FGPTNFLNEVIWKRTSAHSSARRYGPIHDVLLFYSRGERHCWNAQYQPYDAEYLDTFFDQ
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCC
EDADGRRWKRTDLTGAGKRNGETGKPWRGIDITAKGRHWSTSPDQLDALDLAGKVHWPKK
CCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCHH
AGGMPRLRQYPEDLPGVALQDIWTDIKPLHNRAAERLHYPTQKPVSLLDRILRSSANVGE
CCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCH
VVLDPFCGCGTTIAAAQLAGRQWIGIDVAYHAIRVIEDRLADMPGKIDYDLGGIPRDFAS
HHHCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AARLAERDKYQFQWWANYLVGVQALKEIRKGPDRGIDGQMYFMNGPRGWGRVLTSVKGGQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCC
HIGTKDVREFKAVIDRERAEMGLFICLREPTRDMRSEAASFGFVDTAHGHLPRLQIVSIE
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEEEEH
RWFNGERPTIPSLGHLSREFFRQSSKPEKSKKPRRPDPTAPEFPFSFIGDGKDAVVHFNP
HHCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCHHCCCCCCEEEEECC
TAVPKEHGLSEGLL
CCCCHHCCCCCCCC
>Mature Secondary Structure 
SRARIAAGAADQGGRWPGQWLTAYQRSSCKVSFAAVGPEMKNQLWFGDNLTILREEIAD
CCCCEECCCCCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHCCEEECCCHHHHHHHHCC
ESVDLVYLDPPFNSQVNYNVLFRTPDEDAASAQVEAFRDTWTWGPEAKWAFDEIMHTGGG
CCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
VTPIVHALHAALGDSDMMAYLVMMAQRLHELRRVLRPTGSLYLHCDPTASHYLKIILDAI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
FGPTNFLNEVIWKRTSAHSSARRYGPIHDVLLFYSRGERHCWNAQYQPYDAEYLDTFFDQ
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCC
EDADGRRWKRTDLTGAGKRNGETGKPWRGIDITAKGRHWSTSPDQLDALDLAGKVHWPKK
CCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCHH
AGGMPRLRQYPEDLPGVALQDIWTDIKPLHNRAAERLHYPTQKPVSLLDRILRSSANVGE
CCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCH
VVLDPFCGCGTTIAAAQLAGRQWIGIDVAYHAIRVIEDRLADMPGKIDYDLGGIPRDFAS
HHHCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AARLAERDKYQFQWWANYLVGVQALKEIRKGPDRGIDGQMYFMNGPRGWGRVLTSVKGGQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCC
HIGTKDVREFKAVIDRERAEMGLFICLREPTRDMRSEAASFGFVDTAHGHLPRLQIVSIE
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEEEEH
RWFNGERPTIPSLGHLSREFFRQSSKPEKSKKPRRPDPTAPEFPFSFIGDGKDAVVHFNP
HHCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCHHCCCCCCEEEEECC
TAVPKEHGLSEGLL
CCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]