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The map label for this gene is mcbR [H]

Identifier: 13488057

GI number: 13488057

Start: 28360

End: 29049

Strand: Reverse

Name: mcbR [H]

Synonym: mll9027

Alternate gene names: 13488057

Gene position: 29049-28360 (Counterclockwise)

Preceding gene: 13488058

Following gene: 13488056

Centisome position: 8.25

GC content: 57.1

Gene sequence:

>690_bases
ATGCCTGCCACCACTTTACCATCGCTGAAGCCGCGCCAGACCAATTCGGACCTCGAGCACGTCTACGAGGAGCTGAAGCG
GTCGATAATGATGGCGGAGTTTTCACCGGGCCAGAAGCTAAAATTGGAGGAACTTGCGAACGCTTTTGGGACGAGCCACA
TGCCGGTTCGTGAAGCGCTCAACCGCCTTGTAATGGCACGCGCGCTCGAGTCAGCGCCGCGCCGTTCAATGTTCATCCCG
GACGCCAGTGTCGAGCGACTTCGGAACCTTTTGACGTTGCGCACGGATCTTGAGGGCAAGGCCGTTGGGATGACAGTCGC
ACAGAGACTTCCGAACCTCGCGGACATTTTGGCGCAAATCAACGCCAGAATGGATGTGGAAGCAGCCCGCGGCGCTCTGG
GGACAAGGACCTATCTTCAGCTCAATCACCGATTCCATTTTGCCCTTTACGAGCGTTGCGGCAACCCGGAGCTCGTAAAC
CTCATTGAACTTTTGTGGATGCGCTACGGTCCGCTGCTCAGCCTGCTAAAGACCACACCAATATCTTTCTCAGGGCATCG
GCACCATGCTGAGATAATTGATGCGGTTCGGGAGGGTGACAAAGACTCCGCCATCAAGAGCCTGGTCGCGGACTTGGAGG
AAGCCGGCAACGCAATCGCCAGTGCGGACGCATTCAGGCCATCCACATAG

Upstream 100 bases:

>100_bases
TCGCGATTGTCCGCTAGCGTCAAGAAAACTCTCTTTGATCGTTGATCAAGCAATGCGTTCGCGATATGTGTGACTGGCCT
TGTCTTCTCCTGGAGTCACA

Downstream 100 bases:

>100_bases
CGGGTGGCCTACGGCATTTCTACGCCGAATGACGACTGGCATAGTCAGTGTGCCGTCCAGAATTATCTGTCTCTGTCCGG
AATTATGCCGGGCTTAGAAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPATTLPSLKPRQTNSDLEHVYEELKRSIMMAEFSPGQKLKLEELANAFGTSHMPVREALNRLVMARALESAPRRSMFIP
DASVERLRNLLTLRTDLEGKAVGMTVAQRLPNLADILAQINARMDVEAARGALGTRTYLQLNHRFHFALYERCGNPELVN
LIELLWMRYGPLLSLLKTTPISFSGHRHHAEIIDAVREGDKDSAIKSLVADLEEAGNAIASADAFRPST

Sequences:

>Translated_229_residues
MPATTLPSLKPRQTNSDLEHVYEELKRSIMMAEFSPGQKLKLEELANAFGTSHMPVREALNRLVMARALESAPRRSMFIP
DASVERLRNLLTLRTDLEGKAVGMTVAQRLPNLADILAQINARMDVEAARGALGTRTYLQLNHRFHFALYERCGNPELVN
LIELLWMRYGPLLSLLKTTPISFSGHRHHAEIIDAVREGDKDSAIKSLVADLEEAGNAIASADAFRPST
>Mature_228_residues
PATTLPSLKPRQTNSDLEHVYEELKRSIMMAEFSPGQKLKLEELANAFGTSHMPVREALNRLVMARALESAPRRSMFIPD
ASVERLRNLLTLRTDLEGKAVGMTVAQRLPNLADILAQINARMDVEAARGALGTRTYLQLNHRFHFALYERCGNPELVNL
IELLWMRYGPLLSLLKTTPISFSGHRHHAEIIDAVREGDKDSAIKSLVADLEEAGNAIASADAFRPST

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=204, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25513; Mature: 25382

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATTLPSLKPRQTNSDLEHVYEELKRSIMMAEFSPGQKLKLEELANAFGTSHMPVREAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHH
NRLVMARALESAPRRSMFIPDASVERLRNLLTLRTDLEGKAVGMTVAQRLPNLADILAQI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHH
NARMDVEAARGALGTRTYLQLNHRFHFALYERCGNPELVNLIELLWMRYGPLLSLLKTTP
HCCCCHHHHHCCCCCHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ISFSGHRHHAEIIDAVREGDKDSAIKSLVADLEEAGNAIASADAFRPST
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure 
PATTLPSLKPRQTNSDLEHVYEELKRSIMMAEFSPGQKLKLEELANAFGTSHMPVREAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHH
NRLVMARALESAPRRSMFIPDASVERLRNLLTLRTDLEGKAVGMTVAQRLPNLADILAQI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHH
NARMDVEAARGALGTRTYLQLNHRFHFALYERCGNPELVNLIELLWMRYGPLLSLLKTTP
HCCCCHHHHHCCCCCHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ISFSGHRHHAEIIDAVREGDKDSAIKSLVADLEEAGNAIASADAFRPST
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]