BacMap

Definition	Mesorhizobium loti MAFF303099 chromosome, complete genome.
Accession	NC_002678
Length	7,036,071

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The map label for this gene is crt [H]

Identifier: 13476731

GI number: 13476731

Start: 6687199

End: 6688074

Strand: Direct

Name: crt [H]

Synonym: mlr8134

Alternate gene names: 13476731

Gene position: 6687199-6688074 (Clockwise)

Preceding gene: 13476728

Following gene: 13476735

Centisome position: 95.04

GC content: 66.55

Gene sequence:

>876_bases
ATGACCGATCTTGTCCTGAACGAGACCCGTGACGGGGTGAGTGTCCTCACCCTCAACCGCCCGGAAAAGCTCAACGCGCT
GAACTACGCCCTGATCGATCGCCTGCTCGCGGTTCTCGACGACATAGAAGTCGATGGCAGCGTCCGGGCTGTCATCCTCA
CCGGAGCAGGGGAGCGGGCGTTTTCGGCGGGTGGCGATATCCATGAATTCTCGGCTAGCGTGGCCCACGGTACGGATGTG
GCGCTGCGCGACTTCGTCATGCGCGGCCAACGGCTGACGGCAAGGCTCGAAGCCTTCCGCAAGCCCATCATCGCCGCCGT
CAACGGAATTGCCTTCGGCGGCGGCTGCGAGATCACAGAGGCCGTGCCGCTGGCCGTCGCCAGCGACCGTGCCCTGTTCG
CCAAGCCGGAGATCAACCTCGCAATGCCGCCGACCTTCGGCGGCACGCAGCGCCTGCCGCGGCTCGCCGGGCGCAAACGC
GCGCTGGAACTGCTTTTGACGGGCGCAACCTTTTCGGCTGAACGAGCGGCTGAACTCGGCCTCGTCAACAAGATTGTGCC
GCATGCCGAGCTGATGCCGGCTGCACACGATCTTGCCCGCCGCATCGTCACGCATTCGCCGGCAGCGCTCGCTGGCATTC
TCACCGCGGTGGCGCGCGGCATCAACCTCGGCATCGCCGAAGGGCTGTTGGTCGAGGCTGAGCAGTTCGCCCGCATGGCC
CCGACCGCCGATCTCAGGGAAGGGCTTGGCGCCTGGATCGAGCGGCGCAGGCCCAGCTATGACGGCTCCTGGACGCACAT
CGCGAGGCCGGACGAGACAAGGCGCGCCTCGCTGCGGCTCGATCAGCCTGTGGGGCAGGCCAAAGCGTCGAGATGA

Upstream 100 bases:

>100_bases
CGGGATGACAGCTTCAGGCATCGGGCATGTATACCGACTAGTGAATATACACGCAATCGAAAGCATGATCCCTTTCCGTC
GTATCTGGAAAGGAAGAACA

Downstream 100 bases:

>100_bases
GGCGTCACGTCGGGCGCAGCGTGCTGCCTGGCGGCCGTCACTTCGCCCTGACCTGGCCAGGTCGACAATGCCGAAGATGA
TAACCATCGGCAGCAGCCTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTDLVLNETRDGVSVLTLNRPEKLNALNYALIDRLLAVLDDIEVDGSVRAVILTGAGERAFSAGGDIHEFSASVAHGTDV
ALRDFVMRGQRLTARLEAFRKPIIAAVNGIAFGGGCEITEAVPLAVASDRALFAKPEINLAMPPTFGGTQRLPRLAGRKR
ALELLLTGATFSAERAAELGLVNKIVPHAELMPAAHDLARRIVTHSPAALAGILTAVARGINLGIAEGLLVEAEQFARMA
PTADLREGLGAWIERRRPSYDGSWTHIARPDETRRASLRLDQPVGQAKASR

Sequences:

>Translated_291_residues
MTDLVLNETRDGVSVLTLNRPEKLNALNYALIDRLLAVLDDIEVDGSVRAVILTGAGERAFSAGGDIHEFSASVAHGTDV
ALRDFVMRGQRLTARLEAFRKPIIAAVNGIAFGGGCEITEAVPLAVASDRALFAKPEINLAMPPTFGGTQRLPRLAGRKR
ALELLLTGATFSAERAAELGLVNKIVPHAELMPAAHDLARRIVTHSPAALAGILTAVARGINLGIAEGLLVEAEQFARMA
PTADLREGLGAWIERRRPSYDGSWTHIARPDETRRASLRLDQPVGQAKASR
>Mature_290_residues
TDLVLNETRDGVSVLTLNRPEKLNALNYALIDRLLAVLDDIEVDGSVRAVILTGAGERAFSAGGDIHEFSASVAHGTDVA
LRDFVMRGQRLTARLEAFRKPIIAAVNGIAFGGGCEITEAVPLAVASDRALFAKPEINLAMPPTFGGTQRLPRLAGRKRA
LELLLTGATFSAERAAELGLVNKIVPHAELMPAAHDLARRIVTHSPAALAGILTAVARGINLGIAEGLLVEAEQFARMAP
TADLREGLGAWIERRRPSYDGSWTHIARPDETRRASLRLDQPVGQAKASR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=37.5478927203065, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=31.6, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=29.1828793774319, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=32.183908045977, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI150378541, Length=257, Percent_Identity=30.3501945525292, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI37594469, Length=194, Percent_Identity=33.5051546391753, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI37594471, Length=194, Percent_Identity=33.5051546391753, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI68989263, Length=177, Percent_Identity=32.2033898305085, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=183, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI45643119, Length=202, Percent_Identity=26.7326732673267, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI260275230, Length=202, Percent_Identity=26.7326732673267, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI25453481, Length=255, Percent_Identity=23.921568627451, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538573, Length=255, Percent_Identity=23.921568627451, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=242, Percent_Identity=24.3801652892562, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=242, Percent_Identity=24.3801652892562, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757968, Length=255, Percent_Identity=23.5294117647059, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI49355787, Length=255, Percent_Identity=23.5294117647059, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=25.3275109170306, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=25.3275109170306, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=37.4045801526718, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=34.3396226415094, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=34.7014925373134, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=30.5343511450382, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=35.1485148514851, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=27.9693486590038, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=31.3432835820896, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=36.6141732283465, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=36.3636363636364, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=33.1896551724138, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=34.0659340659341, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=200, Percent_Identity=33.5, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=34.0659340659341, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=200, Percent_Identity=33.5, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=205, Percent_Identity=32.1951219512195, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=208, Percent_Identity=32.2115384615385, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=31.9371727748691, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=31.3131313131313, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=27.8260869565217, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=260, Percent_Identity=28.4615384615385, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=219, Percent_Identity=27.3972602739726, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=27.3469387755102, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=30.6930693069307, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=39.1129032258064, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=39.1129032258064, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=34.0996168582375, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI28571730, Length=255, Percent_Identity=28.2352941176471, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571729, Length=255, Percent_Identity=28.2352941176471, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922422, Length=272, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=25.9541984732824, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=27.4774774774775, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24654903, Length=190, Percent_Identity=30, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=31.5508021390374, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24650670, Length=256, Percent_Identity=26.953125, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=31.5508021390374, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=31.5508021390374, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=33.1632653061224, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 31141; Mature: 31010

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLVLNETRDGVSVLTLNRPEKLNALNYALIDRLLAVLDDIEVDGSVRAVILTGAGERA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHH
FSAGGDIHEFSASVAHGTDVALRDFVMRGQRLTARLEAFRKPIIAAVNGIAFGGGCEITE
HCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCHHH
AVPLAVASDRALFAKPEINLAMPPTFGGTQRLPRLAGRKRALELLLTGATFSAERAAELG
HCCEEEECCCEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
LVNKIVPHAELMPAAHDLARRIVTHSPAALAGILTAVARGINLGIAEGLLVEAEQFARMA
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
PTADLREGLGAWIERRRPSYDGSWTHIARPDETRRASLRLDQPVGQAKASR
CCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHEEECCCCCCCCCCC
>Mature Secondary Structure 
TDLVLNETRDGVSVLTLNRPEKLNALNYALIDRLLAVLDDIEVDGSVRAVILTGAGERA
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHH
FSAGGDIHEFSASVAHGTDVALRDFVMRGQRLTARLEAFRKPIIAAVNGIAFGGGCEITE
HCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCHHH
AVPLAVASDRALFAKPEINLAMPPTFGGTQRLPRLAGRKRALELLLTGATFSAERAAELG
HCCEEEECCCEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
LVNKIVPHAELMPAAHDLARRIVTHSPAALAGILTAVARGINLGIAEGLLVEAEQFARMA
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
PTADLREGLGAWIERRRPSYDGSWTHIARPDETRRASLRLDQPVGQAKASR
CCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]