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The map label for this gene is cysS

Identifier: 13476470

GI number: 13476470

Start: 6453126

End: 6454559

Strand: Direct

Name: cysS

Synonym: mlr7799

Alternate gene names: 13476470

Gene position: 6453126-6454559 (Clockwise)

Preceding gene: 13476468

Following gene: 13476471

Centisome position: 91.71

GC content: 62.97

Gene sequence:

>1434_bases
ATGTCCGACGCATCGCAGGGCCTGCGCCTCTACAACACACTGACACGTGCGAAGACGGACTTCGTTCCGATCGATGCGCT
CAATGTGCGCATGTATGTCTGCGGCCCGACGGTCTACGACTTCGCCCATATCGGCAATGCCAGGCCGGTTATCGTCTTCG
ACGTGCTGTTCCGCCTGCTACGCCATCTCTATGGGCAGGCGCATGTCACCTATGTGCGCAACATCACCGACGTCGACGAC
AAGATCAACGCGCGTGCGCTGCGCGATTTCGGTAGCGAGATATCGGCCGGAAAGCTGTCGCTCAACGAAGCCATCCGGCG
GGTGACCGAAAAGACCGCCGACCAGTTCCACAGGGATGTCGCCACGCTCGGCTGCCTTGAGCCGACGGTCGAGCCGCGCG
CCACCGAATTCGTCGAGCCACGCGCCGACGGCAAGGCCGACATGATCACCTTGATTCAAAGCCTGATCAAACGCGGCCAT
GCCTATGCCGCGGCAGGCGAAGTTCTATTCGACACCGCGTCGATGCCGGACTACGGACAATTGTCGAAGCGCAATCTCGA
CGAACAGCAGGCCGGCGCCCGCGTCGCCGTCGACGCGCACAAGAAGAACCCTGGCGATTTCGTGCTGTGGAAGCTGTCAT
CGCCGGAAGAACCGGGCTGGCAGAGCCCATGGGGCAGGGGCCGGCCGGGCTGGCACATCGAATGCTCGGCGATGTCGGCA
GCCTATCTCGGCGAGGTGTTCGACATCCATGGCGGCGGCCTCGATCTCATCTTCCCGCATCATGAGAACGAGATCGCCCA
GTCGCGCTGCGCCCACGGCACATCAGTCATGGCCAATGTCTGGATGCACAACGGCTTCCTGCAGGTCGAAGGGCAGAAGA
TGTCGAAGAGCCTCGGCAATTTCTACTCGATCCACGAACTGCTGGAGACGGAGACCTTCGGCGGCCGCAGCTGGCCCGGC
GAGGTGCTGCGGCTGGCGATGCTGATGACGCACTACCGCGAGCCGATCGACTTTTCCGTGCGCAAGCTGGAGGAGGCCGA
GAACACGCTGCGCAAATGGAAACGCGCCGCGGATCTGGCGCCTGCGGCAGGGCAGTTGCCGGTGGAGGTGATCGATGCCT
TGTCGGACGATCTTGCCACCTATGCCGCCTTCCAGGTGCTGACGCAGTTGGCCGGCGAGGCCGCGGACGGCAACGAAGCC
GCCGCTGCGCTGAAGGCGTCGCTGCTGTTCCTCGGCTTCGATGTCGCCTCGGCCGACATCGATGAAGATGGTGTTGCCAA
GGCGATCGCCAATCGCCTCGCGCTCATCGCCGCAAAGAACTGGACCGAGGCCGACCGCATCCGCGACGAGTTGCTGGCGC
AAGGTGTACAGTTGAAAGACGGCAAGGACCCCGTCACGGGCGAACGCATCACCACCTGGGAGGTAAAAAGATGA

Upstream 100 bases:

>100_bases
GAGTGGACAAACGCCCCGGCCAGCAGGCTGGAGTCTGATTTGCGCCTCGGCGCGGGCCTGGCTCCATCCAGATAGAAGTT
CTGAACCGGGAAGGCATGGA

Downstream 100 bases:

>100_bases
TCGACCATCTCGGCATAACCGTGGCCGATTTCGATGCTTCGAAGGCTTTCTACGACAAGGCGATGGCTCCGCTTGGCGCC
TTGCTCCTTTACATGGTGCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSDASQGLRLYNTLTRAKTDFVPIDALNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGQAHVTYVRNITDVDD
KINARALRDFGSEISAGKLSLNEAIRRVTEKTADQFHRDVATLGCLEPTVEPRATEFVEPRADGKADMITLIQSLIKRGH
AYAAAGEVLFDTASMPDYGQLSKRNLDEQQAGARVAVDAHKKNPGDFVLWKLSSPEEPGWQSPWGRGRPGWHIECSAMSA
AYLGEVFDIHGGGLDLIFPHHENEIAQSRCAHGTSVMANVWMHNGFLQVEGQKMSKSLGNFYSIHELLETETFGGRSWPG
EVLRLAMLMTHYREPIDFSVRKLEEAENTLRKWKRAADLAPAAGQLPVEVIDALSDDLATYAAFQVLTQLAGEAADGNEA
AAALKASLLFLGFDVASADIDEDGVAKAIANRLALIAAKNWTEADRIRDELLAQGVQLKDGKDPVTGERITTWEVKR

Sequences:

>Translated_477_residues
MSDASQGLRLYNTLTRAKTDFVPIDALNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGQAHVTYVRNITDVDD
KINARALRDFGSEISAGKLSLNEAIRRVTEKTADQFHRDVATLGCLEPTVEPRATEFVEPRADGKADMITLIQSLIKRGH
AYAAAGEVLFDTASMPDYGQLSKRNLDEQQAGARVAVDAHKKNPGDFVLWKLSSPEEPGWQSPWGRGRPGWHIECSAMSA
AYLGEVFDIHGGGLDLIFPHHENEIAQSRCAHGTSVMANVWMHNGFLQVEGQKMSKSLGNFYSIHELLETETFGGRSWPG
EVLRLAMLMTHYREPIDFSVRKLEEAENTLRKWKRAADLAPAAGQLPVEVIDALSDDLATYAAFQVLTQLAGEAADGNEA
AAALKASLLFLGFDVASADIDEDGVAKAIANRLALIAAKNWTEADRIRDELLAQGVQLKDGKDPVTGERITTWEVKR
>Mature_476_residues
SDASQGLRLYNTLTRAKTDFVPIDALNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHLYGQAHVTYVRNITDVDDK
INARALRDFGSEISAGKLSLNEAIRRVTEKTADQFHRDVATLGCLEPTVEPRATEFVEPRADGKADMITLIQSLIKRGHA
YAAAGEVLFDTASMPDYGQLSKRNLDEQQAGARVAVDAHKKNPGDFVLWKLSSPEEPGWQSPWGRGRPGWHIECSAMSAA
YLGEVFDIHGGGLDLIFPHHENEIAQSRCAHGTSVMANVWMHNGFLQVEGQKMSKSLGNFYSIHELLETETFGGRSWPGE
VLRLAMLMTHYREPIDFSVRKLEEAENTLRKWKRAADLAPAAGQLPVEVIDALSDDLATYAAFQVLTQLAGEAADGNEAA
AALKASLLFLGFDVASADIDEDGVAKAIANRLALIAAKNWTEADRIRDELLAQGVQLKDGKDPVTGERITTWEVKR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=337, Percent_Identity=39.4658753709199, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI21269877, Length=244, Percent_Identity=39.344262295082, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI303304987, Length=244, Percent_Identity=39.344262295082, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI62240992, Length=244, Percent_Identity=39.344262295082, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI10835051, Length=244, Percent_Identity=39.344262295082, Blast_Score=188, Evalue=1e-47,
Organism=Escherichia coli, GI1786737, Length=412, Percent_Identity=44.4174757281553, Blast_Score=333, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17509795, Length=251, Percent_Identity=39.8406374501992, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17509797, Length=251, Percent_Identity=39.8406374501992, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324082, Length=360, Percent_Identity=35.2777777777778, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI19922218, Length=373, Percent_Identity=36.7292225201072, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI19922364, Length=273, Percent_Identity=37.3626373626374, Blast_Score=185, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_RHILO (Q984X8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_108040.1
- ProteinModelPortal:   Q984X8
- SMR:   Q984X8
- GeneID:   1230692
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7799
- NMPDR:   fig|266835.1.peg.6139
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52642; Mature: 52511

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDASQGLRLYNTLTRAKTDFVPIDALNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLL
CCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHH
RHLYGQAHVTYVRNITDVDDKINARALRDFGSEISAGKLSLNEAIRRVTEKTADQFHRDV
HHHHCCHHEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ATLGCLEPTVEPRATEFVEPRADGKADMITLIQSLIKRGHAYAAAGEVLFDTASMPDYGQ
HHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHCHHHEECCCCCCCCC
LSKRNLDEQQAGARVAVDAHKKNPGDFVLWKLSSPEEPGWQSPWGRGRPGWHIECSAMSA
HHHCCCCHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHH
AYLGEVFDIHGGGLDLIFPHHENEIAQSRCAHGTSVMANVWMHNGFLQVEGQKMSKSLGN
HHHHHHHEECCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHCCCEEEECCHHHHHHHHH
FYSIHELLETETFGGRSWPGEVLRLAMLMTHYREPIDFSVRKLEEAENTLRKWKRAADLA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PAAGQLPVEVIDALSDDLATYAAFQVLTQLAGEAADGNEAAAALKASLLFLGFDVASADI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEHHCCCC
DEDGVAKAIANRLALIAAKNWTEADRIRDELLAQGVQLKDGKDPVTGERITTWEVKR
CCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
SDASQGLRLYNTLTRAKTDFVPIDALNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLL
CCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHH
RHLYGQAHVTYVRNITDVDDKINARALRDFGSEISAGKLSLNEAIRRVTEKTADQFHRDV
HHHHCCHHEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ATLGCLEPTVEPRATEFVEPRADGKADMITLIQSLIKRGHAYAAAGEVLFDTASMPDYGQ
HHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHCHHHEECCCCCCCCC
LSKRNLDEQQAGARVAVDAHKKNPGDFVLWKLSSPEEPGWQSPWGRGRPGWHIECSAMSA
HHHCCCCHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHH
AYLGEVFDIHGGGLDLIFPHHENEIAQSRCAHGTSVMANVWMHNGFLQVEGQKMSKSLGN
HHHHHHHEECCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHCCCEEEECCHHHHHHHHH
FYSIHELLETETFGGRSWPGEVLRLAMLMTHYREPIDFSVRKLEEAENTLRKWKRAADLA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PAAGQLPVEVIDALSDDLATYAAFQVLTQLAGEAADGNEAAAALKASLLFLGFDVASADI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEHHCCCC
DEDGVAKAIANRLALIAAKNWTEADRIRDELLAQGVQLKDGKDPVTGERITTWEVKR
CCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968