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The map label for this gene is gcvA [C]

Identifier: 13476453

GI number: 13476453

Start: 6440275

End: 6441192

Strand: Reverse

Name: gcvA [C]

Synonym: mll7779

Alternate gene names: 13476453

Gene position: 6441192-6440275 (Counterclockwise)

Preceding gene: 13476458

Following gene: 13476434

Centisome position: 91.55

GC content: 67.86

Gene sequence:

>918_bases
ATGGTCACATCTCCATCGCTCAAAGGACTTCAGGCTTTCGAGGCCGCGGCGCGCGCGGGCAGCTTTGCCGCGGCGGCGGA
AGAGCTTTCGGTCTCGGCGGCTGCTGTCAGCCAGCTTATCCGCACCGTCGAGGAACAGATGGGCCGCAAGCTGTTTCATC
GGGTCAACCGCAAGGTTGTGCTGACCGAGGCCGGAGTGGAAATACTGCCACGGTTGACCATGGCCTTCCAGGAAATCGGC
AGTGTCTCGCGCGAATTGGACGGCGATGCCTTCCGCCCGCGCCTGGTTGTATCGGTGCCGCCTTCGATGGCGATGGGCTG
GCTTTCGCAGCGTCTGGCGGGCTTCGTCGCAAGCCACGGCGCCGCCGACATCTCGCTGCGCGGCGACGACGACCCGGTGC
CGTTCGATCGCGAACTGATCGATATCAGGCTCTCCTATGGTCCGCACTATCGCGAGCACCCTACCGAGGACATCGCCCGG
GACGCCGTCTATCCCGTCTGCGCACCGGGGCTTGCCAGCGCGATCAAGGCGGACGGCGGTCCGCTCGCCGGGCTGCCGCT
GATCCACACCGACTGGGGACCGACCGGCGCCTCGTTTCCGTCCTGGCGCAACTGGTTCGAGGCTGAAGGTGTCCAGCCTG
GCCGGGCAGCGCAGCGGGGCCTGTCCGCCAACTCGTCGCGCGCCGCCCTCGACCTTGCCATTTCGGGGCTCGGCGTCACA
CTGGCGCAAGGGGTCTATTGTGCCGAGGCGCTGGAAGACGGCAGGCTGGTCCGGCCCGCCGCCAGGGTGATCGCGCTGCG
CCAGCCCTATTGCCTGACCATTCCGGAGCGCAGCGCCAGGCGCGATGTCGTGGCGGCGTTCCGCGACTGGCTGATCGAGG
AATGCCGTCGCGCTGTGGGCTCGCCGGCGCTGCGTTGA

Upstream 100 bases:

>100_bases
GGCTGGCGACTTGATAAGGCCTATTCTAGTTGGTGAAAAAACTTTTCAAAAACGACGATTCCTGCGATTTTGATAAGTCC
ATCTTATCTATCTGGCGATT

Downstream 100 bases:

>100_bases
CCCGTCAGGCTCGCGTGGTCAGAGGTGCCCGCTCAAGGCCGCCACCATGTCCTTGCTGGTCGCCACCGGAATGATCTCGA
ACTCAACCAGATCGGCCCAT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MVTSPSLKGLQAFEAAARAGSFAAAAEELSVSAAAVSQLIRTVEEQMGRKLFHRVNRKVVLTEAGVEILPRLTMAFQEIG
SVSRELDGDAFRPRLVVSVPPSMAMGWLSQRLAGFVASHGAADISLRGDDDPVPFDRELIDIRLSYGPHYREHPTEDIAR
DAVYPVCAPGLASAIKADGGPLAGLPLIHTDWGPTGASFPSWRNWFEAEGVQPGRAAQRGLSANSSRAALDLAISGLGVT
LAQGVYCAEALEDGRLVRPAARVIALRQPYCLTIPERSARRDVVAAFRDWLIEECRRAVGSPALR

Sequences:

>Translated_305_residues
MVTSPSLKGLQAFEAAARAGSFAAAAEELSVSAAAVSQLIRTVEEQMGRKLFHRVNRKVVLTEAGVEILPRLTMAFQEIG
SVSRELDGDAFRPRLVVSVPPSMAMGWLSQRLAGFVASHGAADISLRGDDDPVPFDRELIDIRLSYGPHYREHPTEDIAR
DAVYPVCAPGLASAIKADGGPLAGLPLIHTDWGPTGASFPSWRNWFEAEGVQPGRAAQRGLSANSSRAALDLAISGLGVT
LAQGVYCAEALEDGRLVRPAARVIALRQPYCLTIPERSARRDVVAAFRDWLIEECRRAVGSPALR
>Mature_305_residues
MVTSPSLKGLQAFEAAARAGSFAAAAEELSVSAAAVSQLIRTVEEQMGRKLFHRVNRKVVLTEAGVEILPRLTMAFQEIG
SVSRELDGDAFRPRLVVSVPPSMAMGWLSQRLAGFVASHGAADISLRGDDDPVPFDRELIDIRLSYGPHYREHPTEDIAR
DAVYPVCAPGLASAIKADGGPLAGLPLIHTDWGPTGASFPSWRNWFEAEGVQPGRAAQRGLSANSSRAALDLAISGLGVT
LAQGVYCAEALEDGRLVRPAARVIALRQPYCLTIPERSARRDVVAAFRDWLIEECRRAVGSPALR

Specific function: Regulatory Protein For The Glycine Cleavage System Operon (Gcv). Mediates Activation Of Gcv By Glycine And Repression By Purines. Gcva Is Negatively Autoregulated. Bind To Three Sites Upstream Of The Gcv Promoter. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=32.6530612244898, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=31.1643835616438, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=27.6094276094276, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787128, Length=144, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32776; Mature: 32776

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSPSLKGLQAFEAAARAGSFAAAAEELSVSAAAVSQLIRTVEEQMGRKLFHRVNRKVV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LTEAGVEILPRLTMAFQEIGSVSRELDGDAFRPRLVVSVPPSMAMGWLSQRLAGFVASHG
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
AADISLRGDDDPVPFDRELIDIRLSYGPHYREHPTEDIARDAVYPVCAPGLASAIKADGG
CCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCC
PLAGLPLIHTDWGPTGASFPSWRNWFEAEGVQPGRAAQRGLSANSSRAALDLAISGLGVT
CCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
LAQGVYCAEALEDGRLVRPAARVIALRQPYCLTIPERSARRDVVAAFRDWLIEECRRAVG
HHHHHHHHHHHCCCCEECHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
SPALR
CCCCC
>Mature Secondary Structure
MVTSPSLKGLQAFEAAARAGSFAAAAEELSVSAAAVSQLIRTVEEQMGRKLFHRVNRKVV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LTEAGVEILPRLTMAFQEIGSVSRELDGDAFRPRLVVSVPPSMAMGWLSQRLAGFVASHG
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
AADISLRGDDDPVPFDRELIDIRLSYGPHYREHPTEDIARDAVYPVCAPGLASAIKADGG
CCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCC
PLAGLPLIHTDWGPTGASFPSWRNWFEAEGVQPGRAAQRGLSANSSRAALDLAISGLGVT
CCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
LAQGVYCAEALEDGRLVRPAARVIALRQPYCLTIPERSARRDVVAAFRDWLIEECRRAVG
HHHHHHHHHHHCCCCEECHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
SPALR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]