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The map label for this gene is fabI1 [H]

Identifier: 13476200

GI number: 13476200

Start: 6162013

End: 6162831

Strand: Direct

Name: fabI1 [H]

Synonym: mlr7458

Alternate gene names: 13476200

Gene position: 6162013-6162831 (Clockwise)

Preceding gene: 13476199

Following gene: 13476201

Centisome position: 87.58

GC content: 61.42

Gene sequence:

>819_bases
ATGGCGGGTGGACAGGGCCTGATGGCCGGCAAGCGCGGCCTTATTCTTGGCATCGCGAACAACAGGTCGATTGCCTACGG
CATCGCCAAGGCATGCGTCGACCATGGCGCCGAGATTGCGCTGACCTATCAGGGCGAGGCGTTCAAGAAGCGCGTCGAGC
CGTTGGCGGCGGAACTGGGCGCCTTTGTCGCCGGCCATTGCGACGTCACCGATTCGGCGAGCCTCGACGAGGTTTTCGCC
AATGTCGCCAAGCATTGGGGCAAGCTTGATTTCCTCGTCCATGCCATCGCCTTCTCCGACAAGGACGAACTGACCGGCCG
CTATGTCGAGACGACGCGCGACAACTTCCTGCGCACCATGGACATTTCGGTGTTTTCCTTCACCACCATCGCCAAGCGTG
CCGAGGCGCTGATGACCGAGGGCGGTTCATTGCTCACGCTGACCTACTACGGCGCCGAGAAGGTGATGCCGCACTACAAC
GTCATGGGCGTCGCCAAGGCCGCGCTCGAAGCCAGCGTGCGTTATCTCGCCGTCGATCTCGGCGGCAAGAAGATCCGCGT
CAACGCCATATCGGCCGGCCCGATCAAGACGCTGGCGGCCTCGGGCATCGGCGATTTCCGCTACATACTGAAGTGGAACG
AATACAATTCGCCGCTGAAGCAGACGGTCACGCAGGAGGAGGTCGGCGATTCCGGCGTCTATTTCCTGTCCGACCTGTCG
CGTGGCGTCACCGGTGAGGTGCACCATGTCGATTCCGGCTATCACGTGGTCGGTATGAAGGCGGTCGACGCGCCTGATAT
TTCGACGGTCAAGGAATAA

Upstream 100 bases:

>100_bases
CGCTCTTGTCCCCCGAACTGTCCGGTTTGAGCGCTTGAAGGGGCTGTGTGCCTGTGCGATAGGCACTCCACAAAGCACAA
ACCTTGCGGAGAGCGCTCAC

Downstream 100 bases:

>100_bases
CGCCCTCCGCTCATCCTCCGCCAAGGCCCTGGCCCGACCTCCGGAAGACCTGATGTACCCGCTGGTTTATATCGTGCGCC
ACGGCCAGACCGCGTGGAAC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAGGQGLMAGKRGLILGIANNRSIAYGIAKACVDHGAEIALTYQGEAFKKRVEPLAAELGAFVAGHCDVTDSASLDEVFA
NVAKHWGKLDFLVHAIAFSDKDELTGRYVETTRDNFLRTMDISVFSFTTIAKRAEALMTEGGSLLTLTYYGAEKVMPHYN
VMGVAKAALEASVRYLAVDLGGKKIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLKQTVTQEEVGDSGVYFLSDLS
RGVTGEVHHVDSGYHVVGMKAVDAPDISTVKE

Sequences:

>Translated_272_residues
MAGGQGLMAGKRGLILGIANNRSIAYGIAKACVDHGAEIALTYQGEAFKKRVEPLAAELGAFVAGHCDVTDSASLDEVFA
NVAKHWGKLDFLVHAIAFSDKDELTGRYVETTRDNFLRTMDISVFSFTTIAKRAEALMTEGGSLLTLTYYGAEKVMPHYN
VMGVAKAALEASVRYLAVDLGGKKIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLKQTVTQEEVGDSGVYFLSDLS
RGVTGEVHHVDSGYHVVGMKAVDAPDISTVKE
>Mature_271_residues
AGGQGLMAGKRGLILGIANNRSIAYGIAKACVDHGAEIALTYQGEAFKKRVEPLAAELGAFVAGHCDVTDSASLDEVFAN
VAKHWGKLDFLVHAIAFSDKDELTGRYVETTRDNFLRTMDISVFSFTTIAKRAEALMTEGGSLLTLTYYGAEKVMPHYNV
MGVAKAALEASVRYLAVDLGGKKIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLKQTVTQEEVGDSGVYFLSDLSR
GVTGEVHHVDSGYHVVGMKAVDAPDISTVKE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=50.1930501930502, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=29.8804780876494, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789378, Length=263, Percent_Identity=27.7566539923954, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=24.9027237354086, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29214; Mature: 29082

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGQGLMAGKRGLILGIANNRSIAYGIAKACVDHGAEIALTYQGEAFKKRVEPLAAELG
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
AFVAGHCDVTDSASLDEVFANVAKHWGKLDFLVHAIAFSDKDELTGRYVETTRDNFLRTM
HHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHEEEE
DISVFSFTTIAKRAEALMTEGGSLLTLTYYGAEKVMPHYNVMGVAKAALEASVRYLAVDL
ECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHCEEEEEEEC
GGKKIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLKQTVTQEEVGDSGVYFLSDLS
CCCEEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEHHHC
RGVTGEVHHVDSGYHVVGMKAVDAPDISTVKE
CCCCCEEEEECCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AGGQGLMAGKRGLILGIANNRSIAYGIAKACVDHGAEIALTYQGEAFKKRVEPLAAELG
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
AFVAGHCDVTDSASLDEVFANVAKHWGKLDFLVHAIAFSDKDELTGRYVETTRDNFLRTM
HHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHEEEE
DISVFSFTTIAKRAEALMTEGGSLLTLTYYGAEKVMPHYNVMGVAKAALEASVRYLAVDL
ECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHCEEEEEEEC
GGKKIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLKQTVTQEEVGDSGVYFLSDLS
CCCEEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEHHHC
RGVTGEVHHVDSGYHVVGMKAVDAPDISTVKE
CCCCCEEEEECCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]