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The map label for this gene is ispE

Identifier: 13476171

GI number: 13476171

Start: 6133521

End: 6134408

Strand: Reverse

Name: ispE

Synonym: mll7422

Alternate gene names: 13476171

Gene position: 6134408-6133521 (Counterclockwise)

Preceding gene: 13476172

Following gene: 13476170

Centisome position: 87.19

GC content: 67.23

Gene sequence:

>888_bases
GTGGACACCGACATCGGTTCGCGCACCTGGCACGCGCATGCCAAGATCAATCTGGCGCTGCACGTCACCGGCAGGCGTGC
TGACGGCTACCATCTGATCGACAGCCTGGCGGTGTTCACGCGCTTCGGCGACAGGGTCGAGATCGCCCTCGCCGACAGCG
ACGATTTCACCGTATCCGGCCGTTATGCCCCGGCGGTTCCGCTCGACGCCGGCAATCTTGTGCTCAAGGCGCGCGATGCC
TTGCGGCGGGAGGCCGGCTCCGGGCGCACCCCGCCGGTCTCGATAAAGCTGGAAAAGAACCTGCCGATCGCCTCCGGCGT
TGGCGGCGGGTCGAGCGATGCAGCGGCCGTGTTGCGCGGACTGGCACAGACATGGGCCCTGGACATCGACGACGCCGGGC
TGGCGCGGATCGGTCTCTCGCTTGGCGCCGACGTGCCGATGTGCCTTGCGGCAAAGCCGCTGGTGGCGCGCGGCATTGGT
GACGAGCTGTCGATGGTACCGGACTTTTCAGCGCTGGGGCTGGTCCTGGTCAATCCAGGCACGCCGGTCTCGACGGCGGA
TGTCTTCGCGGCCCTGTCCCGCCGCGACAACGAGCCCCTGCCGCCGCTGCCGCGCAGCATCGATTTCCACAGCCTGCGCA
ACTGGCTGGAGGTCACCCGCAATGATCTGGAGCCTGCAGCCCTTGCGCTGCAGCCCGCCATAGGCAGGGCGCTATCATGG
CTCAATAAGGCCGGATCGGGATTTTCACGCATGTCGGGATCGGGCGCGACTTGTTTCGGCCTGTTCGAGACCGGCAATGT
CGCCAAGCGCGCGGCGGCCGAAATCCGCAGCCGCCAGCCCGACTGGTTCGTCGCGGCGACGCGCAGCATGTCATCAGAGG
CTGAGTGA

Upstream 100 bases:

>100_bases
AGGCGGCCAACGGCGCGATGGGAACACTGGTCAAGGACCCGAAGACCGGCGAATACCGGCTGGCCAAGGACTGAGCATCA
TCCCCTATACGACTGACGCC

Downstream 100 bases:

>100_bases
AATGGCTGGGATTAACGAGGGCCGTTCGTTCGTTCCGGTCCGTATCGCGGTGCTGACGGTTTCGGACACGCGCAGCCTTG
CCGACGACAAATCCGGCCAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDTDIGSRTWHAHAKINLALHVTGRRADGYHLIDSLAVFTRFGDRVEIALADSDDFTVSGRYAPAVPLDAGNLVLKARDA
LRREAGSGRTPPVSIKLEKNLPIASGVGGGSSDAAAVLRGLAQTWALDIDDAGLARIGLSLGADVPMCLAAKPLVARGIG
DELSMVPDFSALGLVLVNPGTPVSTADVFAALSRRDNEPLPPLPRSIDFHSLRNWLEVTRNDLEPAALALQPAIGRALSW
LNKAGSGFSRMSGSGATCFGLFETGNVAKRAAAEIRSRQPDWFVAATRSMSSEAE

Sequences:

>Translated_295_residues
MDTDIGSRTWHAHAKINLALHVTGRRADGYHLIDSLAVFTRFGDRVEIALADSDDFTVSGRYAPAVPLDAGNLVLKARDA
LRREAGSGRTPPVSIKLEKNLPIASGVGGGSSDAAAVLRGLAQTWALDIDDAGLARIGLSLGADVPMCLAAKPLVARGIG
DELSMVPDFSALGLVLVNPGTPVSTADVFAALSRRDNEPLPPLPRSIDFHSLRNWLEVTRNDLEPAALALQPAIGRALSW
LNKAGSGFSRMSGSGATCFGLFETGNVAKRAAAEIRSRQPDWFVAATRSMSSEAE
>Mature_295_residues
MDTDIGSRTWHAHAKINLALHVTGRRADGYHLIDSLAVFTRFGDRVEIALADSDDFTVSGRYAPAVPLDAGNLVLKARDA
LRREAGSGRTPPVSIKLEKNLPIASGVGGGSSDAAAVLRGLAQTWALDIDDAGLARIGLSLGADVPMCLAAKPLVARGIG
DELSMVPDFSALGLVLVNPGTPVSTADVFAALSRRDNEPLPPLPRSIDFHSLRNWLEVTRNDLEPAALALQPAIGRALSW
LNKAGSGFSRMSGSGATCFGLFETGNVAKRAAAEIRSRQPDWFVAATRSMSSEAE

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=291, Percent_Identity=33.6769759450172, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_RHILO (Q986C6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107741.1
- ProteinModelPortal:   Q986C6
- SMR:   Q986C6
- GeneID:   1230396
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll7422
- NMPDR:   fig|266835.1.peg.5842
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK14608
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31133; Mature: 31133

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: ACT_SITE 15-15 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTDIGSRTWHAHAKINLALHVTGRRADGYHLIDSLAVFTRFGDRVEIALADSDDFTVSG
CCCCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEC
RYAPAVPLDAGNLVLKARDALRREAGSGRTPPVSIKLEKNLPIASGVGGGSSDAAAVLRG
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCCCHHHHHHHHH
LAQTWALDIDDAGLARIGLSLGADVPMCLAAKPLVARGIGDELSMVPDFSALGLVLVNPG
HHHHHEECCCCCCHHHHHHHHCCCCCHHHHCCCHHHHCCCCCHHCCCCCCCCCEEEECCC
TPVSTADVFAALSRRDNEPLPPLPRSIDFHSLRNWLEVTRNDLEPAALALQPAIGRALSW
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LNKAGSGFSRMSGSGATCFGLFETGNVAKRAAAEIRSRQPDWFVAATRSMSSEAE
HHHCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MDTDIGSRTWHAHAKINLALHVTGRRADGYHLIDSLAVFTRFGDRVEIALADSDDFTVSG
CCCCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEC
RYAPAVPLDAGNLVLKARDALRREAGSGRTPPVSIKLEKNLPIASGVGGGSSDAAAVLRG
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCCCHHHHHHHHH
LAQTWALDIDDAGLARIGLSLGADVPMCLAAKPLVARGIGDELSMVPDFSALGLVLVNPG
HHHHHEECCCCCCHHHHHHHHCCCCCHHHHCCCHHHHCCCCCHHCCCCCCCCCEEEECCC
TPVSTADVFAALSRRDNEPLPPLPRSIDFHSLRNWLEVTRNDLEPAALALQPAIGRALSW
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LNKAGSGFSRMSGSGATCFGLFETGNVAKRAAAEIRSRQPDWFVAATRSMSSEAE
HHHCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968