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The map label for this gene is gcvA [H]

Identifier: 13476153

GI number: 13476153

Start: 6114885

End: 6115793

Strand: Reverse

Name: gcvA [H]

Synonym: mll7397

Alternate gene names: 13476153

Gene position: 6115793-6114885 (Counterclockwise)

Preceding gene: 13476156

Following gene: 13476152

Centisome position: 86.92

GC content: 66.78

Gene sequence:

>909_bases
ATGCTGAACCGCGTCCACCTCAACGGCCTTCGCGCCGTCGAAACCGTTGCCCGTCTCGGCTCGCTGGCGGCGGCCGCCGG
CGAGCTCAACGTTTCCGTCAGTGCCGTCAGCCAGCAGATAAGCCGCACCGAAAAGCAGCTCGGCCAAGCGCTGTTCGAGC
GCACGGCGAGCGGCCTGGTGCTGACCGAGTTCGGCGCGGCCTTCGCCACCCGCCTCGGCGCTGGATTTCGCGAGCTTGCC
CAGGCCGTGGCGCTGGCCGACGAGGCGAGCCAATGCACATTGGTCGTCTCGGTGGCGCCAGCCTTCGCTTCGAAATGGCT
GCTGCCCAGACTGTCGCGTCACTTCGACCGCCATCCCAATGTGCTGCTGCGCATCGATGCGTCGGTGCGGCTGGCCAATC
TCGATCATTCCGACGTCGACATCGCCATCCGGCTTGGCGACGGCGAATGGCCGGGCGTGCGCGCGGAACTGCTGCTGGCG
CAGGAGGTCTTCCCGGTCTGCGCGCCGTCGATCGCGGCCAAGCTCAGGTCGATCGAGGATCTGGCGCAGACCTGCGCGAT
CACCGACGAGCGGTCGATGATCAGCTGGGACAGCTGGTTCAAAGCGGCGGGCGTCGAGCCGGTCACCTTCCTCAAGGGCG
CGCGCTTCACCGACCCGATGCTGTGCCTGGAATCGGCGATCGCCGGCCATGGCGTGATGCTCGCCTGGCAATTGCTGACA
GCCGACGCTCTCACCGACGGCCGGCTGGTGGCGCCTTTCGGCGTGCGCGCCGAGAGCGGGCTGGGCTACTGGATGGTAAC
CTCGGCGACCAAGGCCGAAAGCCGCAAGGTGCACGATTTCAAAGCCTGGATCCGCGAAGAAATCGAGACGACAATGGCGC
AGTTCCGAACGCTCGACAGCGCCGCTTAA

Upstream 100 bases:

>100_bases
GCGGCTCCTCGGATCTGGTTCCCATCGATAGAGTCTGCCGTTCCCTCGCGTCTTTCAATTGAGATTTCAGCCGGATCAAT
TTAGAAAATCTCAAATGGCC

Downstream 100 bases:

>100_bases
GCCTGGTCCACCGCAAACCTGGTGGAAAGGATCGTGGCAGCGGTCTATGTATGGGGCAGACTTCCACATCACGGCAGGCA
GGATCATGACCACACATTCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MLNRVHLNGLRAVETVARLGSLAAAAGELNVSVSAVSQQISRTEKQLGQALFERTASGLVLTEFGAAFATRLGAGFRELA
QAVALADEASQCTLVVSVAPAFASKWLLPRLSRHFDRHPNVLLRIDASVRLANLDHSDVDIAIRLGDGEWPGVRAELLLA
QEVFPVCAPSIAAKLRSIEDLAQTCAITDERSMISWDSWFKAAGVEPVTFLKGARFTDPMLCLESAIAGHGVMLAWQLLT
ADALTDGRLVAPFGVRAESGLGYWMVTSATKAESRKVHDFKAWIREEIETTMAQFRTLDSAA

Sequences:

>Translated_302_residues
MLNRVHLNGLRAVETVARLGSLAAAAGELNVSVSAVSQQISRTEKQLGQALFERTASGLVLTEFGAAFATRLGAGFRELA
QAVALADEASQCTLVVSVAPAFASKWLLPRLSRHFDRHPNVLLRIDASVRLANLDHSDVDIAIRLGDGEWPGVRAELLLA
QEVFPVCAPSIAAKLRSIEDLAQTCAITDERSMISWDSWFKAAGVEPVTFLKGARFTDPMLCLESAIAGHGVMLAWQLLT
ADALTDGRLVAPFGVRAESGLGYWMVTSATKAESRKVHDFKAWIREEIETTMAQFRTLDSAA
>Mature_302_residues
MLNRVHLNGLRAVETVARLGSLAAAAGELNVSVSAVSQQISRTEKQLGQALFERTASGLVLTEFGAAFATRLGAGFRELA
QAVALADEASQCTLVVSVAPAFASKWLLPRLSRHFDRHPNVLLRIDASVRLANLDHSDVDIAIRLGDGEWPGVRAELLLA
QEVFPVCAPSIAAKLRSIEDLAQTCAITDERSMISWDSWFKAAGVEPVTFLKGARFTDPMLCLESAIAGHGVMLAWQLLT
ADALTDGRLVAPFGVRAESGLGYWMVTSATKAESRKVHDFKAWIREEIETTMAQFRTLDSAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=34.6938775510204, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=31.9587628865979, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788706, Length=301, Percent_Identity=27.906976744186, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=25.2491694352159, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=25.8620689655172, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=216, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=133, Percent_Identity=34.5864661654135, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI87081978, Length=199, Percent_Identity=29.1457286432161, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32658; Mature: 32658

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRVHLNGLRAVETVARLGSLAAAAGELNVSVSAVSQQISRTEKQLGQALFERTASGLV
CCCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTEFGAAFATRLGAGFRELAQAVALADEASQCTLVVSVAPAFASKWLLPRLSRHFDRHPN
EHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCC
VLLRIDASVRLANLDHSDVDIAIRLGDGEWPGVRAELLLAQEVFPVCAPSIAAKLRSIED
EEEEEECCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LAQTCAITDERSMISWDSWFKAAGVEPVTFLKGARFTDPMLCLESAIAGHGVMLAWQLLT
HHHHHCCCCCCCEECHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ADALTDGRLVAPFGVRAESGLGYWMVTSATKAESRKVHDFKAWIREEIETTMAQFRTLDS
HHHCCCCEEECCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AA
CC
>Mature Secondary Structure
MLNRVHLNGLRAVETVARLGSLAAAAGELNVSVSAVSQQISRTEKQLGQALFERTASGLV
CCCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTEFGAAFATRLGAGFRELAQAVALADEASQCTLVVSVAPAFASKWLLPRLSRHFDRHPN
EHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCC
VLLRIDASVRLANLDHSDVDIAIRLGDGEWPGVRAELLLAQEVFPVCAPSIAAKLRSIED
EEEEEECCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LAQTCAITDERSMISWDSWFKAAGVEPVTFLKGARFTDPMLCLESAIAGHGVMLAWQLLT
HHHHHCCCCCCCEECHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ADALTDGRLVAPFGVRAESGLGYWMVTSATKAESRKVHDFKAWIREEIETTMAQFRTLDS
HHHCCCCEEECCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]