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The map label for this gene is mcbR [H]

Identifier: 13476044

GI number: 13476044

Start: 5994165

End: 5994821

Strand: Reverse

Name: mcbR [H]

Synonym: mll7255

Alternate gene names: 13476044

Gene position: 5994821-5994165 (Counterclockwise)

Preceding gene: 13476059

Following gene: 13476043

Centisome position: 85.2

GC content: 62.56

Gene sequence:

>657_bases
GTGCCCCTGCATGAGAAGGTCTATCTGGAGCTTGTGCGGGCGCTGATGTCGGGCCAGTTCACGCCGGGCCAGAAGCTGAC
GTCGCGCAAGCTCGCCAAGGAACTCGGCACCAGCGACATGCCGGTGCGCAGCGCATTCATGCGGCTGCAGGCATTGCGGG
CGCTGAGCCCGATGCCGAACGGCAGTGTCGAGGTGCCTGTCATTTCAGCCGAACGGTTTTCGCAATTGACCGCCGTGCGC
ACGATCCTGGAAGGCTCGGCGACCGAGCTCGCCACAAAACTCGTCAACGGCAACAATCTGCGGACGATCCGACGCCACAG
CAGCGAATTGACCCTGGCGGCGCGCGAGGGCGATATCGACGACTATCTCAGGAAGAACTACGCCTTCAAATTCTCGATCT
ATCGCCATTGCGGCAACGAGCAGATGATCTTCCTGATCGAGACCGTGTGGATGCAGGTCGGCCCCTTCCTCAGGAACCTC
GCCATGGGGTTCGAGGACGATCTCGCCTCCATCCTCGACATCGACTATCACGAGGAAGTCGTCGCCGCGATCGAGGCGCA
GGACGCGGCGCGTGCTCGTGCAGCCATCGTTCGCGACATAGACGAAGGCGCGGCGCACATCCTGCGGCAGGCCAGATTTC
CGGGAGCCAGAGGCTAG

Upstream 100 bases:

>100_bases
GATCATCTGCGGCTGCCGTCGTCAGGCCAGATTTCAGAACGAAGTAGCGCCCCCGCCAATGGCCGACAGGACACAATTCG
GGTTGAGCGCCATCGACAGC

Downstream 100 bases:

>100_bases
GGTTGGAGGGTGATAGCCGCTCGACCCAAGGCCGATCGTGCATGCCGAATGCCCCTTGGCTTTCCGCCTTCTTGCCTCTA
TGTTGCGATCGCAGATCGCG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPLHEKVYLELVRALMSGQFTPGQKLTSRKLAKELGTSDMPVRSAFMRLQALRALSPMPNGSVEVPVISAERFSQLTAVR
TILEGSATELATKLVNGNNLRTIRRHSSELTLAAREGDIDDYLRKNYAFKFSIYRHCGNEQMIFLIETVWMQVGPFLRNL
AMGFEDDLASILDIDYHEEVVAAIEAQDAARARAAIVRDIDEGAAHILRQARFPGARG

Sequences:

>Translated_218_residues
MPLHEKVYLELVRALMSGQFTPGQKLTSRKLAKELGTSDMPVRSAFMRLQALRALSPMPNGSVEVPVISAERFSQLTAVR
TILEGSATELATKLVNGNNLRTIRRHSSELTLAAREGDIDDYLRKNYAFKFSIYRHCGNEQMIFLIETVWMQVGPFLRNL
AMGFEDDLASILDIDYHEEVVAAIEAQDAARARAAIVRDIDEGAAHILRQARFPGARG
>Mature_217_residues
PLHEKVYLELVRALMSGQFTPGQKLTSRKLAKELGTSDMPVRSAFMRLQALRALSPMPNGSVEVPVISAERFSQLTAVRT
ILEGSATELATKLVNGNNLRTIRRHSSELTLAAREGDIDDYLRKNYAFKFSIYRHCGNEQMIFLIETVWMQVGPFLRNLA
MGFEDDLASILDIDYHEEVVAAIEAQDAARARAAIVRDIDEGAAHILRQARFPGARG

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=202, Percent_Identity=27.2277227722772, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24345; Mature: 24214

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHEKVYLELVRALMSGQFTPGQKLTSRKLAKELGTSDMPVRSAFMRLQALRALSPMPN
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
GSVEVPVISAERFSQLTAVRTILEGSATELATKLVNGNNLRTIRRHSSELTLAAREGDID
CCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCHH
DYLRKNYAFKFSIYRHCGNEQMIFLIETVWMQVGPFLRNLAMGFEDDLASILDIDYHEEV
HHHHHCHHHEEHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
VAAIEAQDAARARAAIVRDIDEGAAHILRQARFPGARG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLHEKVYLELVRALMSGQFTPGQKLTSRKLAKELGTSDMPVRSAFMRLQALRALSPMPN
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
GSVEVPVISAERFSQLTAVRTILEGSATELATKLVNGNNLRTIRRHSSELTLAAREGDID
CCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCHH
DYLRKNYAFKFSIYRHCGNEQMIFLIETVWMQVGPFLRNLAMGFEDDLASILDIDYHEEV
HHHHHCHHHEEHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
VAAIEAQDAARARAAIVRDIDEGAAHILRQARFPGARG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]