Click here to switch to the map view.

The map label for this gene is cocE [H]

Identifier: 13475829

GI number: 13475829

Start: 5760367

End: 5762358

Strand: Direct

Name: cocE [H]

Synonym: mlr6999

Alternate gene names: 13475829

Gene position: 5760367-5762358 (Clockwise)

Preceding gene: 13475828

Following gene: 13475830

Centisome position: 81.87

GC content: 62.9

Gene sequence:

>1992_bases
ATGACGAACAGGAATGTCCGTGTCATTGAAAATGAGTGGATCGTCCTGAAAGACGGCACCCGGCTCGCCGCGCGCATCTG
GATGCCCGAAACGGGTCCTGGCGGGGTGCCGGCTGTGCTCGAATTCCTGCCTTATCGCAAACGCAACGGCACCGCCGCGC
GTGACGAGTCCACCTATCCGGTCTTCGCCGCGGCCGGCATCGCGGGCGTGCGTGTCGACATTCGCGGTTGCGGAGAATCC
GACGGCGTGATCGATGGCGAATATACCGCGCGCGAGCTAGCCGACGCCGTCGAGGTTATCGAATGGATCGCTGCGCAGGA
CTGGTCGAACGGCAATGTCGGCATGATGGGAATTTCCTGGGGCGGGTTCAACGCGCTTCAGGTCGCGGCTCTCAAACCAC
CTGCTCTCAAGGCCGTGATTTCACTGTCGTCCACCGTCGACCGCTACAATGACGACATCCACTACAAGAATGGCGCGCAC
CTGTCGGCGCAACTGTCATGGGCGGCGACGATGACTGCGTTGCAGTCGCGGTCCCCCGATCCCGATCTCGTCGGAGAACG
CTGGCGCGACATGTGGCTCGAGCGCCTGGAAGGCGAGCCGTTCTTCATGGAGGAGTGGCTGTCCCACCAACGCCGCGACG
ACTTCTGGCGTCACGGTTCGATCTGCGAGGACTTTGACGGTTTTTCCGTTCCCGCACTGGTGATTGCCGGATGGGCCGAC
GGCTACCGTAACACGCCCTTGAAGGCGGTCGCCGGGATGCCGGAAATGGCCTGGGGCCTGATCGGTCCATGGGTTCACAA
ATATCCGCATTTTGCTGATCCGAAGCCCCGCGTCGATTTTCATGCCGAGGCAATTTCATGGTGGCGGCATTGGCTGTGCG
CAGAAGACAACAAGGTGGAGCATGCGCCGAGGCTGCGTGCCTATATCCTGGACGGGCCGCGACCCGGCCGCCGGCGCGAG
ACCGACCCGGGCTATTGGGTTGCCATGGACAGATGGGACGTCCCCGACACACTCGCCTTTACCTTGGACGCTTCGGGCAG
GCTCGCCCGGAGCACTTCATCGCATGCTGAAGGGAAAGCTCTCCTGCACTCTCCTCAGGATACGGGCACCGCTGCGGGAG
AGTTCTTCCCGCGCAGGCCGGATTCCGAAACGGCCGGCGACCAGCGTGTCGACGATGCTGGCTCGCTCACCTTCGACAGC
GCCGTCCTTGCCGAGGAATGCGTCGTGTTTGGCCAGCCGCTCGCAAAACTGTCGCTTTCCAGTGATGCCAACTTCGCCAA
CCTCGCCGTCAGGCTCGTCGACGTCCATCCCGACGGTGCGGCGACGCGCGTCAGCTACGGTGTGCTCAACCTGGCGCATC
GAAACGGCAATGCCGAACCACAGCCTCTGAAGCCGGGCGAGACGGTCGAAATCGAGTTGGTGCTCGATGCGTGCGGCTAC
CGTTTTGCGCCCGGCCACCGTCTGCGTCTGTCCTTGTCGAGCGCCTATTGGCCGACGATCCTGCCCCAGCCCTGCGACGC
GACGTTGACGATCGATCTTGCCACGCTCCGATTGGAACTGCCGCTGCTTGGCGAGCATCGCAGGGTCGAAATCCCGCAGC
CATCCAATCCCGATCCTCTGCCCCGTTACGAAGTCCTGACAGACGGATCGTCGGACCGCTCTGTTGAACGCGATCTGCAA
AATGGCGTGACGCGCTATCGGGTGCATGAGGACACAGGTCTTAGCCGCCATCCGGGCAACGGCTTGTGCACGCGCGACAT
CCGGCAGGAGGTATGGTCGATAGCGCCCGATAACCCGCTATCGCTGACGGCAGAGATCCGCTGGACGTGCTGTGCCGAGC
GGGAAGGCTGGCGAACGCAGAGCCATTGCACCATGCATCTCTCCTGCACGGCGACCGAATGGGTGGTTACGGAGCGGGTT
GAAGCACTGGAGGCCGGCCGCAAGGTGTTTGAGCGCGAGCGCAATTCCCGCATACCGCGTGACCTCATGTAA

Upstream 100 bases:

>100_bases
CAGCCGGGTCTGGCTGGAGGCTTGAACCGGGAAATGATCGTGCCTTTCCGGCGGCGGGATAGTGTGCTTGCCACCGCGTG
GAGGTTTTGTGGTTGATCGC

Downstream 100 bases:

>100_bases
TAGATCGCCTATCGCTGCGGCTGCGGCAATGCCGGGCATTTCAGCCGGGTGTTTCGCCGGCACACCGGCATCACCCCAAC
CGAGTAAGGCATCCGAATCG

Product: glutaryl 7-ACA acylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MTNRNVRVIENEWIVLKDGTRLAARIWMPETGPGGVPAVLEFLPYRKRNGTAARDESTYPVFAAAGIAGVRVDIRGCGES
DGVIDGEYTARELADAVEVIEWIAAQDWSNGNVGMMGISWGGFNALQVAALKPPALKAVISLSSTVDRYNDDIHYKNGAH
LSAQLSWAATMTALQSRSPDPDLVGERWRDMWLERLEGEPFFMEEWLSHQRRDDFWRHGSICEDFDGFSVPALVIAGWAD
GYRNTPLKAVAGMPEMAWGLIGPWVHKYPHFADPKPRVDFHAEAISWWRHWLCAEDNKVEHAPRLRAYILDGPRPGRRRE
TDPGYWVAMDRWDVPDTLAFTLDASGRLARSTSSHAEGKALLHSPQDTGTAAGEFFPRRPDSETAGDQRVDDAGSLTFDS
AVLAEECVVFGQPLAKLSLSSDANFANLAVRLVDVHPDGAATRVSYGVLNLAHRNGNAEPQPLKPGETVEIELVLDACGY
RFAPGHRLRLSLSSAYWPTILPQPCDATLTIDLATLRLELPLLGEHRRVEIPQPSNPDPLPRYEVLTDGSSDRSVERDLQ
NGVTRYRVHEDTGLSRHPGNGLCTRDIRQEVWSIAPDNPLSLTAEIRWTCCAEREGWRTQSHCTMHLSCTATEWVVTERV
EALEAGRKVFERERNSRIPRDLM

Sequences:

>Translated_663_residues
MTNRNVRVIENEWIVLKDGTRLAARIWMPETGPGGVPAVLEFLPYRKRNGTAARDESTYPVFAAAGIAGVRVDIRGCGES
DGVIDGEYTARELADAVEVIEWIAAQDWSNGNVGMMGISWGGFNALQVAALKPPALKAVISLSSTVDRYNDDIHYKNGAH
LSAQLSWAATMTALQSRSPDPDLVGERWRDMWLERLEGEPFFMEEWLSHQRRDDFWRHGSICEDFDGFSVPALVIAGWAD
GYRNTPLKAVAGMPEMAWGLIGPWVHKYPHFADPKPRVDFHAEAISWWRHWLCAEDNKVEHAPRLRAYILDGPRPGRRRE
TDPGYWVAMDRWDVPDTLAFTLDASGRLARSTSSHAEGKALLHSPQDTGTAAGEFFPRRPDSETAGDQRVDDAGSLTFDS
AVLAEECVVFGQPLAKLSLSSDANFANLAVRLVDVHPDGAATRVSYGVLNLAHRNGNAEPQPLKPGETVEIELVLDACGY
RFAPGHRLRLSLSSAYWPTILPQPCDATLTIDLATLRLELPLLGEHRRVEIPQPSNPDPLPRYEVLTDGSSDRSVERDLQ
NGVTRYRVHEDTGLSRHPGNGLCTRDIRQEVWSIAPDNPLSLTAEIRWTCCAEREGWRTQSHCTMHLSCTATEWVVTERV
EALEAGRKVFERERNSRIPRDLM
>Mature_662_residues
TNRNVRVIENEWIVLKDGTRLAARIWMPETGPGGVPAVLEFLPYRKRNGTAARDESTYPVFAAAGIAGVRVDIRGCGESD
GVIDGEYTARELADAVEVIEWIAAQDWSNGNVGMMGISWGGFNALQVAALKPPALKAVISLSSTVDRYNDDIHYKNGAHL
SAQLSWAATMTALQSRSPDPDLVGERWRDMWLERLEGEPFFMEEWLSHQRRDDFWRHGSICEDFDGFSVPALVIAGWADG
YRNTPLKAVAGMPEMAWGLIGPWVHKYPHFADPKPRVDFHAEAISWWRHWLCAEDNKVEHAPRLRAYILDGPRPGRRRET
DPGYWVAMDRWDVPDTLAFTLDASGRLARSTSSHAEGKALLHSPQDTGTAAGEFFPRRPDSETAGDQRVDDAGSLTFDSA
VLAEECVVFGQPLAKLSLSSDANFANLAVRLVDVHPDGAATRVSYGVLNLAHRNGNAEPQPLKPGETVEIELVLDACGYR
FAPGHRLRLSLSSAYWPTILPQPCDATLTIDLATLRLELPLLGEHRRVEIPQPSNPDPLPRYEVLTDGSSDRSVERDLQN
GVTRYRVHEDTGLSRHPGNGLCTRDIRQEVWSIAPDNPLSLTAEIRWTCCAEREGWRTQSHCTMHLSCTATEWVVTERVE
ALEAGRKVFERERNSRIPRDLM

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 73975; Mature: 73844

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRNVRVIENEWIVLKDGTRLAARIWMPETGPGGVPAVLEFLPYRKRNGTAARDESTYP
CCCCCEEEEECCEEEEECCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
VFAAAGIAGVRVDIRGCGESDGVIDGEYTARELADAVEVIEWIAAQDWSNGNVGMMGISW
EEEECCCEEEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GGFNALQVAALKPPALKAVISLSSTVDRYNDDIHYKNGAHLSAQLSWAATMTALQSRSPD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCC
PDLVGERWRDMWLERLEGEPFFMEEWLSHQRRDDFWRHGSICEDFDGFSVPALVIAGWAD
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
GYRNTPLKAVAGMPEMAWGLIGPWVHKYPHFADPKPRVDFHAEAISWWRHWLCAEDNKVE
CCCCCCHHHHCCCCHHHHHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
HAPRLRAYILDGPRPGRRRETDPGYWVAMDRWDVPDTLAFTLDASGRLARSTSSHAEGKA
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCEECCCCCCCCCCE
LLHSPQDTGTAAGEFFPRRPDSETAGDQRVDDAGSLTFDSAVLAEECVVFGQPLAKLSLS
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHEEECC
SDANFANLAVRLVDVHPDGAATRVSYGVLNLAHRNGNAEPQPLKPGETVEIELVLDACGY
CCCCEEEEEEEEEEECCCCCCCEEHHHHEEEEECCCCCCCCCCCCCCEEEEEEEEECCCC
RFAPGHRLRLSLSSAYWPTILPQPCDATLTIDLATLRLELPLLGEHRRVEIPQPSNPDPL
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCC
PRYEVLTDGSSDRSVERDLQNGVTRYRVHEDTGLSRHPGNGLCTRDIRQEVWSIAPDNPL
CCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
SLTAEIRWTCCAEREGWRTQSHCTMHLSCTATEWVVTERVEALEAGRKVFERERNSRIPR
EEEEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLM
CCC
>Mature Secondary Structure 
TNRNVRVIENEWIVLKDGTRLAARIWMPETGPGGVPAVLEFLPYRKRNGTAARDESTYP
CCCCEEEEECCEEEEECCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
VFAAAGIAGVRVDIRGCGESDGVIDGEYTARELADAVEVIEWIAAQDWSNGNVGMMGISW
EEEECCCEEEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GGFNALQVAALKPPALKAVISLSSTVDRYNDDIHYKNGAHLSAQLSWAATMTALQSRSPD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCC
PDLVGERWRDMWLERLEGEPFFMEEWLSHQRRDDFWRHGSICEDFDGFSVPALVIAGWAD
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
GYRNTPLKAVAGMPEMAWGLIGPWVHKYPHFADPKPRVDFHAEAISWWRHWLCAEDNKVE
CCCCCCHHHHCCCCHHHHHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
HAPRLRAYILDGPRPGRRRETDPGYWVAMDRWDVPDTLAFTLDASGRLARSTSSHAEGKA
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCEECCCCCCCCCCE
LLHSPQDTGTAAGEFFPRRPDSETAGDQRVDDAGSLTFDSAVLAEECVVFGQPLAKLSLS
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHEEECC
SDANFANLAVRLVDVHPDGAATRVSYGVLNLAHRNGNAEPQPLKPGETVEIELVLDACGY
CCCCEEEEEEEEEEECCCCCCCEEHHHHEEEEECCCCCCCCCCCCCCEEEEEEEEECCCC
RFAPGHRLRLSLSSAYWPTILPQPCDATLTIDLATLRLELPLLGEHRRVEIPQPSNPDPL
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCC
PRYEVLTDGSSDRSVERDLQNGVTRYRVHEDTGLSRHPGNGLCTRDIRQEVWSIAPDNPL
CCEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
SLTAEIRWTCCAEREGWRTQSHCTMHLSCTATEWVVTERVEALEAGRKVFERERNSRIPR
EEEEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLM
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]