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The map label for this gene is paaF [C]

Identifier: 13475805

GI number: 13475805

Start: 5736888

End: 5737694

Strand: Direct

Name: paaF [C]

Synonym: mlr6976

Alternate gene names: 13475805

Gene position: 5736888-5737694 (Clockwise)

Preceding gene: 13475804

Following gene: 13475811

Centisome position: 81.54

GC content: 60.47

Gene sequence:

>807_bases
ATGGCCATCCAAATCGACATCACCCGATACGAGAACCTTGCCGTCGAGGCTCATGACGAGGGGGTTTGGGTCGTAACCCT
GAACCGTCCCACCAAACGCAATGCGCTGGATGCAAATACGATCGAAGAACTGGTCGACTTTTTTTCCACCGCTCCTCGCG
CGGGTGTCCGCGCGATTGTGTTGGCCGGGTCGGGTGACCATTTTTGCGCCGGACTAGACTTGATTGAGCATCATCAAGCT
GACCGCAGCCCGGCGGATTTCATGCATATTTGCCTGCGCTGGCACGAGGCCTTCAACAAGATGGAATATGGCGGCGTGCC
GATCATCGCAGCCCTGCAGGGTGCGGTTGTGGGCGGTGGGCTGGAGCTGGCCAGCGCCGCCCATATCCGGGTAATGGACA
AGACGACCTATTTTGCGCTGCCTGAAGGCCAACGTGGACTTTTCACGGGCGGCGGCGCCACGATCCGCGTCACCGATCTG
GTGGGCAAGTCGCGCACGATCGACATGATGCTGACGGGCCGGGTGTATCAGGGACAAGAAGCGGTCGATCTGGGGCTCGC
GCAGTACATCGTCGAAGGATCAAGTTTCGAGAAGGCCCTGGAACTCGCCCACAAAACAGCGCAGAACCTGCCCCTGTCCA
ACTTTGCGATCTGTTCCGCTGTGAGCCACATGCAGAATATGTCCGCGCTGGATGCCGCTTATGCCGAGGCTGTCGTCGCC
GGTGTGGTGAACACTCAGCCCGAGGCCCGGGCTCGACTCGCCGCTTTCGCCGACAAGAGCGCGGCACGGGTGCGGGCCAA
CTATTGA

Upstream 100 bases:

>100_bases
CTTCAGCGAGCCCGGGTCATGGACGGCGCCGCAAGCCTGTTGGACATCGAATTCTGAATAGAACAATCAACCCTCTTCAA
CTCGGGAACAGGACCGTACC

Downstream 100 bases:

>100_bases
GACTGATTCCAGAATTTGTCCCTGGCGGAATTGTGATTTGTAGAGTCGGCAGCGACAGCGCCGCCCAACTCCAGCCATTT
GCGAAAACCGTCGATGCGGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MAIQIDITRYENLAVEAHDEGVWVVTLNRPTKRNALDANTIEELVDFFSTAPRAGVRAIVLAGSGDHFCAGLDLIEHHQA
DRSPADFMHICLRWHEAFNKMEYGGVPIIAALQGAVVGGGLELASAAHIRVMDKTTYFALPEGQRGLFTGGGATIRVTDL
VGKSRTIDMMLTGRVYQGQEAVDLGLAQYIVEGSSFEKALELAHKTAQNLPLSNFAICSAVSHMQNMSALDAAYAEAVVA
GVVNTQPEARARLAAFADKSAARVRANY

Sequences:

>Translated_268_residues
MAIQIDITRYENLAVEAHDEGVWVVTLNRPTKRNALDANTIEELVDFFSTAPRAGVRAIVLAGSGDHFCAGLDLIEHHQA
DRSPADFMHICLRWHEAFNKMEYGGVPIIAALQGAVVGGGLELASAAHIRVMDKTTYFALPEGQRGLFTGGGATIRVTDL
VGKSRTIDMMLTGRVYQGQEAVDLGLAQYIVEGSSFEKALELAHKTAQNLPLSNFAICSAVSHMQNMSALDAAYAEAVVA
GVVNTQPEARARLAAFADKSAARVRANY
>Mature_267_residues
AIQIDITRYENLAVEAHDEGVWVVTLNRPTKRNALDANTIEELVDFFSTAPRAGVRAIVLAGSGDHFCAGLDLIEHHQAD
RSPADFMHICLRWHEAFNKMEYGGVPIIAALQGAVVGGGLELASAAHIRVMDKTTYFALPEGQRGLFTGGGATIRVTDLV
GKSRTIDMMLTGRVYQGQEAVDLGLAQYIVEGSSFEKALELAHKTAQNLPLSNFAICSAVSHMQNMSALDAAYAEAVVAG
VVNTQPEARARLAAFADKSAARVRANY

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=192, Percent_Identity=31.7708333333333, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI194097323, Length=192, Percent_Identity=34.375, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI68989263, Length=184, Percent_Identity=28.804347826087, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=30.6818181818182, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4757968, Length=190, Percent_Identity=23.1578947368421, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI49355787, Length=190, Percent_Identity=23.1578947368421, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=258, Percent_Identity=22.093023255814, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI213417737, Length=255, Percent_Identity=22.3529411764706, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=29.1187739463602, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4757966, Length=190, Percent_Identity=22.6315789473684, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI51538581, Length=190, Percent_Identity=22.6315789473684, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI25453481, Length=190, Percent_Identity=22.6315789473684, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI51538573, Length=190, Percent_Identity=22.6315789473684, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=226, Percent_Identity=32.7433628318584, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI221142681, Length=197, Percent_Identity=32.48730964467, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=113, Percent_Identity=43.3628318584071, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788597, Length=209, Percent_Identity=23.9234449760766, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790281, Length=235, Percent_Identity=21.7021276595745, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=200, Percent_Identity=32.5, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=189, Percent_Identity=32.8042328042328, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=155, Percent_Identity=32.258064516129, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=220, Percent_Identity=28.6363636363636, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=26.5486725663717, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=197, Percent_Identity=30.9644670050761, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=30.241935483871, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357171, Length=201, Percent_Identity=32.8358208955224, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129971, Length=185, Percent_Identity=36.7567567567568, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653477, Length=185, Percent_Identity=36.7567567567568, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24650670, Length=204, Percent_Identity=32.843137254902, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=29.0909090909091, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921000, Length=199, Percent_Identity=32.6633165829146, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583077, Length=199, Percent_Identity=32.6633165829146, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583079, Length=199, Percent_Identity=32.6633165829146, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571729, Length=203, Percent_Identity=28.0788177339901, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571730, Length=203, Percent_Identity=28.0788177339901, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28842; Mature: 28711

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQIDITRYENLAVEAHDEGVWVVTLNRPTKRNALDANTIEELVDFFSTAPRAGVRAIV
CEEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEE
LAGSGDHFCAGLDLIEHHQADRSPADFMHICLRWHEAFNKMEYGGVPIIAALQGAVVGGG
EECCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEECCC
LELASAAHIRVMDKTTYFALPEGQRGLFTGGGATIRVTDLVGKSRTIDMMLTGRVYQGQE
HHHCCCEEEEEEECCEEEECCCCCCCEEECCCCEEEEEEECCCCCEEEEEEECCEECCCH
AVDLGLAQYIVEGSSFEKALELAHKTAQNLPLSNFAICSAVSHMQNMSALDAAYAEAVVA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVVNTQPEARARLAAFADKSAARVRANY
HHHCCCHHHHHHHHHHHCCCCCEEECCC
>Mature Secondary Structure 
AIQIDITRYENLAVEAHDEGVWVVTLNRPTKRNALDANTIEELVDFFSTAPRAGVRAIV
EEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEE
LAGSGDHFCAGLDLIEHHQADRSPADFMHICLRWHEAFNKMEYGGVPIIAALQGAVVGGG
EECCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEECCC
LELASAAHIRVMDKTTYFALPEGQRGLFTGGGATIRVTDLVGKSRTIDMMLTGRVYQGQE
HHHCCCEEEEEEECCEEEECCCCCCCEEECCCCEEEEEEECCCCCEEEEEEECCEECCCH
AVDLGLAQYIVEGSSFEKALELAHKTAQNLPLSNFAICSAVSHMQNMSALDAAYAEAVVA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVVNTQPEARARLAAFADKSAARVRANY
HHHCCCHHHHHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA