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The map label for this gene is gcvA [H]

Identifier: 13475800

GI number: 13475800

Start: 5731314

End: 5732225

Strand: Reverse

Name: gcvA [H]

Synonym: mll6971

Alternate gene names: 13475800

Gene position: 5732225-5731314 (Counterclockwise)

Preceding gene: 13475806

Following gene: 13475799

Centisome position: 81.47

GC content: 59.65

Gene sequence:

>912_bases
ATGAACCGTTACCTCCCGCCTCTGACATGGCTACGCGCATTCGAAGCTTCGGCCCGTACGCTCAGTTTCACCCATGCCGC
CTCCGAATTGCACATCACGCAAGCCGCGGTGTCCAAACATGTGAAATCGTTGGAGCACCATCTGCATCATGTGCTGTTCA
TCCGCCGCCCGCGCGGTCTGGAGCTCACCAAATCTGGCGCCGCCTATCTGCCCAAGGTGCAGGATGCGATGGAACGGCTG
GCTATCGGCACGCGTGAGGTGTTTGGCCAGCGCCGAACCAGTTCGCTGACTGTGCGATGCGCCATTTCCTTCGCCGTCAA
TTGGCTGGCTCAGCGCTTGCCCGAATATCTCGACCGTTATCCGGGCAAGAATATCCGCTTGCTTTCAAGCGTCTGGAATG
ACGCCTTTGACGCGCAGGACTTCGATCTCGACATCCAATACGGCACCGGACACTGGCCTGGGTTCAACAGCCATCGCCTG
ACGTGGGAAACCATCACGCCCCTTTGCGCACCCGAATTGCCTGGCCGCAATCCTCTGAACAGTCCGCAAGACCTGCGTCA
TCATCGTTTGCTGCACGTGCTGGGCTATCATGAAGGCTGGGGCATCTGGCTAAAGGCGGCTGGAGCCCAGCAGGTCGATG
CCGGTTCAGGGTTGCATCTCGACTCCTCCTTGACGGCGTTTGAGTTGGCGGCGGACTGTGCAGGCGTAGCACTGGGCCGC
AGTTCCCTTGCCGGACATGCCCTGGCCTCCGGCCGATTGATAGCGCCGTTTCCTCTCGCGGTGCCTATCGATGAAGCGTT
TCATCTGATCCAACCAGCAGGCAGCAGCCGCCATCCAGATGCCCCGTTGTTTGTCGAATGGCTGCTGTCCGCCGTACAGA
AGTCGGAAAACGCCAACAAGCACGCAAGCTGA

Upstream 100 bases:

>100_bases
TGGTCGACACCCAATGATCAGGCTGGCGCGGTTTCGTCAAAGGACGTATTAGGGGGGCATACCTATACAAAAAGTTATGG
CTGTGTTTTGGAGCGCGCTT

Downstream 100 bases:

>100_bases
ACGTCCATGAGGCAGCGCTCGCGGCGATGACCATCAGCTTCTGCAGCCGCATCAGGTGGTGGCCCCCCGCCAACGGCGAG
GGCCATCCGACGCGATGGAC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNRYLPPLTWLRAFEASARTLSFTHAASELHITQAAVSKHVKSLEHHLHHVLFIRRPRGLELTKSGAAYLPKVQDAMERL
AIGTREVFGQRRTSSLTVRCAISFAVNWLAQRLPEYLDRYPGKNIRLLSSVWNDAFDAQDFDLDIQYGTGHWPGFNSHRL
TWETITPLCAPELPGRNPLNSPQDLRHHRLLHVLGYHEGWGIWLKAAGAQQVDAGSGLHLDSSLTAFELAADCAGVALGR
SSLAGHALASGRLIAPFPLAVPIDEAFHLIQPAGSSRHPDAPLFVEWLLSAVQKSENANKHAS

Sequences:

>Translated_303_residues
MNRYLPPLTWLRAFEASARTLSFTHAASELHITQAAVSKHVKSLEHHLHHVLFIRRPRGLELTKSGAAYLPKVQDAMERL
AIGTREVFGQRRTSSLTVRCAISFAVNWLAQRLPEYLDRYPGKNIRLLSSVWNDAFDAQDFDLDIQYGTGHWPGFNSHRL
TWETITPLCAPELPGRNPLNSPQDLRHHRLLHVLGYHEGWGIWLKAAGAQQVDAGSGLHLDSSLTAFELAADCAGVALGR
SSLAGHALASGRLIAPFPLAVPIDEAFHLIQPAGSSRHPDAPLFVEWLLSAVQKSENANKHAS
>Mature_303_residues
MNRYLPPLTWLRAFEASARTLSFTHAASELHITQAAVSKHVKSLEHHLHHVLFIRRPRGLELTKSGAAYLPKVQDAMERL
AIGTREVFGQRRTSSLTVRCAISFAVNWLAQRLPEYLDRYPGKNIRLLSSVWNDAFDAQDFDLDIQYGTGHWPGFNSHRL
TWETITPLCAPELPGRNPLNSPQDLRHHRLLHVLGYHEGWGIWLKAAGAQQVDAGSGLHLDSSLTAFELAADCAGVALGR
SSLAGHALASGRLIAPFPLAVPIDEAFHLIQPAGSSRHPDAPLFVEWLLSAVQKSENANKHAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=35.7388316151203, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=34.1379310344828, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=30.1694915254237, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33534; Mature: 33534

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYLPPLTWLRAFEASARTLSFTHAASELHITQAAVSKHVKSLEHHLHHVLFIRRPRGL
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
ELTKSGAAYLPKVQDAMERLAIGTREVFGQRRTSSLTVRCAISFAVNWLAQRLPEYLDRY
EEECCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
PGKNIRLLSSVWNDAFDAQDFDLDIQYGTGHWPGFNSHRLTWETITPLCAPELPGRNPLN
CCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCC
SPQDLRHHRLLHVLGYHEGWGIWLKAAGAQQVDAGSGLHLDSSLTAFELAADCAGVALGR
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCH
SSLAGHALASGRLIAPFPLAVPIDEAFHLIQPAGSSRHPDAPLFVEWLLSAVQKSENANK
HHHCCHHHHCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
HAS
CCC
>Mature Secondary Structure
MNRYLPPLTWLRAFEASARTLSFTHAASELHITQAAVSKHVKSLEHHLHHVLFIRRPRGL
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
ELTKSGAAYLPKVQDAMERLAIGTREVFGQRRTSSLTVRCAISFAVNWLAQRLPEYLDRY
EEECCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
PGKNIRLLSSVWNDAFDAQDFDLDIQYGTGHWPGFNSHRLTWETITPLCAPELPGRNPLN
CCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCC
SPQDLRHHRLLHVLGYHEGWGIWLKAAGAQQVDAGSGLHLDSSLTAFELAADCAGVALGR
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCH
SSLAGHALASGRLIAPFPLAVPIDEAFHLIQPAGSSRHPDAPLFVEWLLSAVQKSENANK
HHHCCHHHHCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
HAS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]